Abstract:
:The gap junctional intercellular communication mediated by Cx43 plays indispensable roles in both germ line development and postnatal folliculogenesis. In this study, we focused on the effect of follicle-stimulating hormone (FSH) on the Cx43 protein in rat primary granulosa cells and found that FSH stimulation elevated the phosphorylation in addition to the protein level of Cx43. Serine residues in the carboxyl-terminal region were exclusively phosphorylated in this system and we identified Ser365, Ser368, Ser369 and Ser373 as major phosphorylation sites by FSH stimulation. A Cx43 variant containing mutations at all these serine residues was found to severely reduce dye transfer activity when assayed in HeLa cells. The present study revealed a novel regulatory mechanism of Cx43-mediated gap junctional intercellular communication through phosphorylation in the carboxyl-terminus.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Yogo K,Ogawa T,Akiyama M,Ishida N,Takeya Tdoi
10.1016/s0014-5793(02)03441-5keywords:
subject
Has Abstractpub_date
2002-11-06 00:00:00pages
132-6issue
2eissn
0014-5793issn
1873-3468pii
S0014579302034415journal_volume
531pub_type
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