Identification of the phosphorylation sites of H2B histone by a catalytic fragment of p72syk from porcine spleen.

abstract：:Flavonoid 3'-hydroxylase (F3'H) and flavonoid 3',5'-hydroxylase (F3'5'H) are cytochrome P450 enzymes and determine the B-ring hydroxylation pattern of flavonoids by introducing hydroxyl groups at the 3'- or the 3'- and 5'-position, respectively. Sequence identity between F3'H and F3'5'H is generally low since their di...

journal_title：FEBS letters

authors： Seitz C,Ameres S,Forkmann G

更新日期：2007-07-24 00:00:00

abstract：:Recently, the existence and extended diversity of antimicrobial peptides has been revealed in two mussel species. These molecules are classified into four groups according to common features of their primary structure: defensins, mytilins, myticins and mytimycin. In Mytilus galloprovincialis, gene structure reveals sy...

journal_title：FEBS letters

authors： Mitta G,Vandenbulcke F,Roch P

更新日期：2000-12-15 00:00:00

abstract：:Autophagy in the yeast is similar to that in mammalian cells. A mutant designated as apg1 (autophagy) defective in accumulation of autophagic bodies in the vacuoles was isolated by selection using a light microscope from a mutagenized proteinase-deficient strain. In the apg1 strain, which has normal vacuolar proteinas...

journal_title：FEBS letters

authors： Tsukada M,Ohsumi Y

更新日期：1993-10-25 00:00:00

abstract：:Culturing human fibroblasts in a three-dimensional collagen matrix leads to a reduction of collagen I by more than 90%, both on the level of mRNA steady-state as well as protein. In order to differentiate changes in de novo transcription and posttranscriptional control, nuclear run on assays and pulse/chase experiment...

journal_title：FEBS letters

authors： Eckes B,Mauch C,Hüppe G,Krieg T

更新日期：1993-03-01 00:00:00

abstract：:The cytochrome bc(1) complex catalyzes electron transfer from ubiquinol to cytochrome c by a protonmotive Q cycle mechanism in which electron transfer is linked to proton translocation across the inner mitochondrial membrane. In the Q cycle mechanism proton translocation is the net result of topographically segregated...

journal_title：FEBS letters

authors： Hunte C,Palsdottir H,Trumpower BL

更新日期：2003-06-12 00:00:00

abstract：:When the eicosapentaenoic acid (EPA)-deficient mutant strain IK-1Delta8 of the marine EPA-producing Shewanella marinintestina IK-1 was treated with various concentrations of hydrogen peroxide (H(2)O(2)), its colony-forming ability decreased more than that of the wild type. Protein carbonylation, induced by treating ce...

journal_title：FEBS letters

authors： Nishida T,Morita N,Yano Y,Orikasa Y,Okuyama H

更新日期：2007-09-04 00:00:00

abstract：:Laser flash-induced photovoltage responses of the D85S and D85T mutants as well as of the wild-type acid blue form are similar and reflect intraprotein charge redistribution caused by retinal isomerization. The Cl- -induced transition of all of these blue forms into purple ones is accompanied by the appearance of elec...

journal_title：FEBS letters

authors： Kalaidzidis IV,Kaulen AD

更新日期：1997-12-01 00:00:00

abstract：:A 3.3 kb cDNA encoding the complete amino acid sequence of a calcium/calmodulin regulated protein phosphatase has been isolated from a Drosophila eye disc cDNA library. The predicted protein of 560 amino acids (molecular mass 62 kDa) is 73-78% identical to human PP2B isoforms. The cDNA hybridised to the X-chromosome a...

journal_title：FEBS letters

authors： Brown L,Chen MX,Cohen PT

更新日期：1994-02-14 00:00:00

abstract：:Mutations in the human mtDNA gene encoding subunit III of cytochrome c oxidase (CO) have been reported to cause MELAS and LHON. Poracoccus denitrificans cells expressing substitutions homologous to these MELAS- and LHON-causing mutations had lower growth yield than wild type cells and lower efficiency of proton pumpin...

journal_title：FEBS letters

authors： Mather MW,Rottenberg H

更新日期：1998-08-14 00:00:00

abstract：:The formation of cytoplasmic inclusion bodies by Escherichia coli overproducing Clostridium thermocellum endoglucanase D (EGD) was investigated. EGD was found in inclusion bodies as a 68 kDa form, whereas the size of the cytoplasmic form was 65 kDa. Upon solubilization with urea followed by dialysis, the 68 kDa form w...

journal_title：FEBS letters

authors： Tokatlidis K,Dhurjati P,Millet J,Béguin P,Aubert JP

更新日期：1991-04-22 00:00:00

abstract：:Angiotensin II (Ang II), a potent hypertrophic factor for vascular smooth muscle cells (VSMC), induces activation of the ras proto-oncogene product (Ras) and mitogen-activated protein (MAP) kinases, and tyrosine phosphorylation of a focal adhesion-associated protein, paxillin. Forskolin, a direct activator of adenylat...

journal_title：FEBS letters

authors： Takahashi T,Kawahara Y,Okuda M,Yokoyama M

更新日期：1996-11-11 00:00:00

abstract：:The Fanconi anemia (FA) pathway, of which the FANCD2 protein is a key component, plays crucial roles in the maintenance of hematopoietic stem cells and suppression of carcinogenesis. However, the function of FANCD2 remains unclear. Here, we report that FANCD2 is a novel and specific substrate of caspase 3. Cleavage of...

journal_title：FEBS letters

authors： Sakai W,Sugasawa K

更新日期：2014-10-16 00:00:00

abstract：:γ-Glutamyl kinase (GK; the PRO1 gene product) is a key enzyme in the Saccharomyces cerevisiae proline biosynthesis pathway. Δpro1 cells are more sensitive to various stresses than wild-type cells, suggesting that GK has an alternative function independent of proline biosynthesis. We show that PRO1 genetically interact...

journal_title：FEBS letters

authors： Tatehashi Y,Watanabe D,Takagi H

更新日期：2016-09-01 00:00:00

abstract：:Mycobacterium avium subsp. hominissuis (MAH) is an environmental bacterium causing opportunistic infections. The objective of this study was to identify flexible genome regions in MAH isolated from different sources. By comparing five complete and draft MAH genomes we identified a genomic island conferring additional ...

journal_title：FEBS letters

authors： Lahiri A,Sanchini A,Semmler T,Schäfer H,Lewin A

更新日期：2014-11-03 00:00:00

abstract：:The dependence of protein splicing on conserved residues of the Cne PRP8 intein was assessed by alanine scanning mutagenesis in a foreign protein context. Corroboration was obtained for the involvement of residues at the splice junctions and of the conserved threonine and histidine of motif B. Five additional residues...

journal_title：FEBS letters

authors： Pearl EJ,Tyndall JD,Poulter RT,Wilbanks SM

更新日期：2007-06-26 00:00:00

abstract：:We performed microarray and computational gene network analyses to identify the detailed mechanisms by which sodium butyrate (SB) induces cell growth arrest and the differentiation of mouse colonic epithelial MCE301 cells. Two thousand six hundred four differentially expressed probe sets were identified in the cells t...

journal_title：FEBS letters

authors： Tabuchi Y,Takasaki I,Doi T,Ishii Y,Sakai H,Kondo T

更新日期：2006-05-29 00:00:00

abstract：:Mesothelial cells, which cover the surface of visceral organs and serous cavities in mammals, play a crucial role in preventing adhesion. We previously reported that primary mesothelial progenitor cells (MPCs) can not only prevent postoperative adhesion but also promote liver regeneration after hepatectomy. Induced pl...

journal_title：FEBS letters

authors： Inagaki NF,Inagaki FF,Kokudo N,Miyajima A

更新日期：2019-02-01 00:00:00

abstract：:The Pb, Eu and Mg-induced cleavages in E. coli and lupine tRNAPhe have been characterized and compared with those found in yeast tRNAPhe. The pattern of lupine tRNAPhe hydrolysis closely resembles that of yeast tRNAPhe, while several major differences occur in the specificity and efficiency of the E. coli tRNAPhe hydr...

journal_title：FEBS letters

authors： Marciniec T,Ciesiołka J,Wrzesinski J,Krzyzosiak WJ

更新日期：1989-01-30 00:00:00

abstract：:VinJ is an amidohydrolase belonging to the serine peptidase family that catalyzes the hydrolysis of the terminal aminoacyl moiety of a polyketide intermediate during the biosynthesis of vicenistatin. Herein, we report the crystal structure of VinJ. VinJ possesses a unique hydrophobic tunnel for the recognition of the ...

journal_title：FEBS letters

authors： Shinohara Y,Miyanaga A,Kudo F,Eguchi T

更新日期：2014-03-18 00:00:00

abstract：:Rho family small GTPases regulate lymphocyte migration induced by chemokines. However, how lymphocyte migration is regulated by Rho GTPases remains to be elucidated. Here, we identified FilGAP, a Rac-specific GAP, as a negative regulator of lymphocyte polarization and migration. Depletion of FilGAP in mouse pro-B BAF ...

journal_title：FEBS letters

authors： Iida T,Saito K,Katagiri K,Kinashi T,Ohta Y

更新日期：2016-05-01 00:00:00

abstract：:Extracellular regulated kinases (ERKs) mediate the inhibitory effect of tumor necrosis factor alpha (TNF-alpha) on uncoupling protein-1 (UCP-1), but not on lipid accumulation. TNF-alpha-induced ERK-dependent peroxisome proliferator activator receptor gamma (PPAR gamma) phosphorylation could be responsible for UCP-1 do...

journal_title：FEBS letters

authors： Porras A,Valladares A,Alvarez AM,Roncero C,Benito M

更新日期：2002-06-05 00:00:00

abstract：:Incubation of human thrombocytes in the presence of [3H]palmitic acid leads to incorporation of this fatty acid into the alpha subunit of Gs as described [Linder et al., Proc. Natl. Acad. Sci. USA 90 (1993) 3675-3679; Degtyarev et al., Biochemistry 32 (1993) 8057-8061] but also into the catalyst of adenylyl cyclase wh...

journal_title：FEBS letters

authors： Mollner S,Beck K,Pfeuffer T

更新日期：1995-09-11 00:00:00

abstract：:Prostaglandin E2 receptors (EPR), which belong to the family of heterotrimeric G protein-coupled ectoreceptors with seven transmembrane domains, can be classified into four subtypes according to their ligand binding and G protein coupling specificity. Of these, EP3betaR is coupled to Gi, whereas EP4R is coupled to Gs....

journal_title：FEBS letters

authors： Neuschäfer-Rube F,Hänecke K,Püschel GP

更新日期：1997-09-29 00:00:00

abstract：:Gangliosides and neutral glycolipids of muscles from normal and dystrophic chickens were studied. Total glycolipid content of the degenerating muscles was higher than the normal muscles. In addition, the myopathic muscles contained a ganglioside which was absent in the unaffected muscles from normal and dystrophic chi...

journal_title：FEBS letters

authors： Kundu SK,Misra LK,Luthra MG

更新日期：1982-12-27 00:00:00

abstract：:Replacing the essential Cys-149 by a selenocysteine into the active site of phosphorylating glyceraldehyde 3-phosphate dehydrogenase (GAPDH) from Bacillus stearothermophilus leads to a selenoGAPDH that mimics a selenoperoxidase activity. Saturation kinetics were observed with cumenyl and tert-butyl hydroperoxides, wit...

journal_title：FEBS letters

authors： Boschi-Muller S,Muller S,Van Dorsselaer A,Böck A,Branlant G

更新日期：1998-11-20 00:00:00

abstract：:Statistically significant similarity was revealed between amino acid sequences of NTP-binding pattern-containing domains which are among the most conserved protein segments in dissimilar groups of ss and dsDNA viruses (papova-, parvo-, geminiviruses and P4 bacteriophage), and RNA viruses (picorna-, como- and nepovirus...

journal_title：FEBS letters

authors： Gorbalenya AE,Koonin EV,Wolf YI

更新日期：1990-03-12 00:00:00

abstract：:The involvement of reactive oxygen species in Ca(2+)-induced mitochondrial membrane permeabilization and cell viability was studied using yeast cells in which the thioredoxin peroxidase (TPx) gene was disrupted and/or catalase was inhibited by 3-amino-1,2, 4-triazole (ATZ) treatment. Wild-type Saccharomyces cerevisiae...

journal_title：FEBS letters

authors： Kowaltowski AJ,Vercesi AE,Rhee SG,Netto LE

更新日期：2000-05-12 00:00:00

abstract：:In the filamentous fungus Neurospora, the production of dsRNA can elicit a dsRNA-induced transcriptional response similar to the interferon response in vertebrates. However, how fungi sense the expression of dsRNA and activate gene expression is unknown. In this study, we established a dsRNA response reporter system i...

journal_title：FEBS letters

authors： Li X,Shen Y,Sun J,Wang B,He Q

更新日期：2011-03-23 00:00:00

abstract：:Early in the course of pregnancy, at the preimplantation stage, the pig embryo is likely to exert a paracrine effect on the tissue intended to receive it, via the secretion of interferons. Our observations show that trophoblastic interferons induce an increase of some mRNAs in the epithelial cells of the gilt endometr...

journal_title：FEBS letters

authors： Chwetzoff S,d'Andrea S

更新日期：1997-03-24 00:00:00

abstract：:Inositol 1,4,5-trisphosphate (1,4,5-InsP3) was perfused into rat dorsal root ganglion (DRG) neurons by whole-cell patch-clamp electrodes, while measuring the membrane potential. This operation evoked a transient (2-3 min) membrane hyperpolarization of about -15 mV (from -42 mV) followed by a depolarization. The membra...

journal_title：FEBS letters

authors： Wang Z,Ypey DL,Van Duijn B

更新日期：1992-06-15 00:00:00