Abstract:
:The dependence of protein splicing on conserved residues of the Cne PRP8 intein was assessed by alanine scanning mutagenesis in a foreign protein context. Corroboration was obtained for the involvement of residues at the splice junctions and of the conserved threonine and histidine of motif B. Five additional residues were identified as absolutely required for splicing. Variant W151A displayed premature C-terminal cleavage, not seen with other Cne PRP8 mutants. We propose a model whereby W151 acts to prevent premature C-terminal cleavage, favoring complete splicing as opposed to two disjointed cleavage events.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Pearl EJ,Tyndall JD,Poulter RT,Wilbanks SMdoi
10.1016/j.febslet.2007.05.060subject
Has Abstractpub_date
2007-06-26 00:00:00pages
3000-4issue
16eissn
0014-5793issn
1873-3468pii
S0014-5793(07)00596-0journal_volume
581pub_type
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