Transcriptional activator, AoXlnR, mediates cellulose-inductive expression of the xylanolytic and cellulolytic genes in Aspergillus oryzae.

abstract：:Astrocytes (AC) induce blood-brain barrier (BBB) properties in brain endothelial cells (EC). As antioxidative activity (AOA) is assumed to be a BBB characteristic, we tested whether AC improve AOA of EC. Monocultivated AC showed higher AOA [manganese superoxide dismutase (SOD), catalase (Cat), glutathione peroxidase (...

journal_title：FEBS letters

authors： Schroeter ML,Mertsch K,Giese H,Müller S,Sporbert A,Hickel B,Blasig IE

更新日期：1999-04-23 00:00:00

abstract：:Cytosolic Ca2+ concentration and membrane potential were monitored in individual cultured endothelial cells mechanically stimulated with a micropipette attached to the stage of a microscope. Both dimpling and poking of endothelial cells resulted in Ca2+i transients (from 63 +/- 12 to 397 +/- 52 nM, characterized by a ...

journal_title：FEBS letters

authors： Goligorsky MS

更新日期：1988-11-21 00:00:00

abstract：:Stable CHO cell clones which selectively express all three rat tachykinin receptors were established by transfection. The binding of radiolabled substance P and neurokinin A (substance K) to CHO clones expressing the NK1 and NK2 receptors, respectively, were saturatable and of high affinity (Kd = 0.17 nM (NK1); 3.4 nM...

journal_title：FEBS letters

authors： Gether U,Marray T,Schwartz TW,Johansen TE

更新日期：1992-01-27 00:00:00

abstract：:It has long been known from fluorescence recovery after photobleaching experiments that the mobility of most cell surface receptors is much smaller than expected for free diffusion of proteins in a fluid lipid bilayer. Single-particle tracking experiments are currently revealing the complexity of the constraints to fr...

journal_title：FEBS letters

authors： Cherry RJ,Smith PR,Morrison IE,Fernandez N

更新日期：1998-06-23 00:00:00

abstract：:Nuclear proteins are actively and posttranslationally transported across the nuclear envelope. This transport is a highly selective process that can be divided into two steps, receptor-binding followed by translocation through the nuclear envelope. Receptor-binding is mediated by nuclear localization signals that have...

journal_title：FEBS letters

authors： Wagner P,Kunz J,Koller A,Hall MN

更新日期：1990-11-26 00:00:00

abstract：:In fat-cells, insulin increases synergistically the inhibitory effects of adenosine and prostaglandin E2 on adenylyl cyclase activity. When the endogenous production of these feedback inhibitors is suppressed, insulin is no more active in conditions where glycosyl inositol-phosphate which is a putative mediator of its...

journal_title：FEBS letters

authors： Martiny L,Dib K,Haye B,Corrèze C,Jacquemin C,Lambert B

更新日期：1991-07-29 00:00:00

abstract：:When acquiring internal membranes and vesicular transport, eukaryotic cells started to synthesize sphingolipids and sterols. The physical differences between these and the glycerophospholipids must have enabled the cells to segregate lipids in the membrane plane. Localizing this event to the Golgi then allowed them to...

journal_title：FEBS letters

authors： van Meer G,Hoetzl S

更新日期：2010-05-03 00:00:00

abstract：:The half-lives of mRNAs in yeast vary from about 1 to over 100 min. While mRNA stabilities must strongly influence overall gene expression in this organism, very little is known about how they are determined. Labellings of yeast cells were conducted to investigate whether the 5'-cap structures of yeast mRNAs might inf...

journal_title：FEBS letters

authors： Piper PW,Curran B,Davies W,Hirst K,Seward K

更新日期：1987-08-10 00:00:00

abstract：:Biotops with extreme temperatures such as deserts force animals to avoid or escape high temperatures by biochemical, behavioural or morphological adaptation. In this context we tested the resistance to heat of the oxygen carrier hemocyanin from the ancient tarantula Eurypelma californicum, which is found in arid zones...

journal_title：FEBS letters

authors： Sterner R,Vogl T,Hinz HJ,Penz F,Hoff R,Föll R,Decker H

更新日期：1995-05-01 00:00:00

abstract：:Cellular ageing is characterized by the accumulation of oxidatively modified proteins which may be due to increased protein damage and/or decreased elimination of oxidized protein. Since the proteasome is in charge of protein turnover and removal of oxidized protein, its fate during ageing and upon oxidative stress ha...

journal_title：FEBS letters

authors： Friguet B

更新日期：2006-05-22 00:00:00

abstract：:Two alpha-type CK2-activated PKAs (CK2-aPKAIalpha and CK2-aPKAIIalpha) were biochemically characterized in vitro using GST-HBV core fusion protein (GST-Hcore) and GST-Hcore157B as phosphate acceptors. It was found that (i), in the absence of cAMP, these two CK2-aPKAs phosphorylated both Ser-170 and Ser-178 on GST-Hcor...

journal_title：FEBS letters

authors： Enomoto M,Sawano Y,Kosuge S,Yamano Y,Kuroki K,Ohtsuki K

更新日期：2006-02-06 00:00:00

abstract：:We describe here a 70 kDa transcription factor A1F-B, which preferentially binds to an element encompassing a CCAAT motif on the rat aldolase B promoter. Comparison of binding specificities, relative molecular masses, and subunit compositions with those of other known CCAAT-binding factors indicated that A1F-B is a no...

journal_title：FEBS letters

authors： Tsutsumi K,Ito K,Yabuki T,Ishikawa K

更新日期：1993-04-19 00:00:00

abstract：:vp165 (or gp160) is an aminopeptidase that has been identified as one of the major proteins of the GLUT4-containing vesicles. In the present study we have determined the degree of co-localization between vp165 and GLUT4 in rat adipose cells and used perturbation by wortmannin to assess the exocytic and endocytic steps...

journal_title：FEBS letters

authors： Malide D,St-Denis JF,Keller SR,Cushman SW

更新日期：1997-06-16 00:00:00

abstract：:The crawling locomotion and shape of eukaryotic cells have been associated with the stochastic molecular dynamics of actin and its protein regulators, chiefly Arp2/3 and Rho family GTPases, in making a cytoskeleton meshwork within cell extensions. However, the cell's actin-dependent oscillatory shape and extension dyn...

journal_title：FEBS letters

authors： Vicker MG

更新日期：2002-01-02 00:00:00

abstract：:The diffusion of fluorescein isothiocyanate-labelled dextran molecules in suspensions of centrifugally, tightly packed, erythrocyte ghosts was measured by fluorescence recovery after photobleaching. In comparison with diffusion in aqueous solution, the diffusion coefficients for probe molecules of varying size were ab...

journal_title：FEBS letters

authors： Järnefelt J,Laurent T,Rigler R

更新日期：1988-12-19 00:00:00

abstract：:Disulfide bridges were introduced into CrylAa, a Bacillus thuringiensis lepidopteran toxin, to stabilize different protein domains including domain I alpha-helical regions thought to be involved in membrane integration and permeation. Bridged mutants could not form functional ion channels in lipid bilayers in the oxid...

journal_title：FEBS letters

authors： Schwartz JL,Juteau M,Grochulski P,Cygler M,Préfontaine G,Brousseau R,Masson L

更新日期：1997-06-30 00:00:00

abstract：:Hell's Gate globin I (HGbI), a heme-containing protein structurally homologous to mammalian neuroglobins, has been identified from an acidophilic and thermophilic obligate methanotroph, Methylacidiphilum infernorum. HGbI has very high affinity for O(2) and shows barely detectable autoxidation in the pH range of 5.2-8....

journal_title：FEBS letters

authors： Teh AH,Saito JA,Baharuddin A,Tuckerman JR,Newhouse JS,Kanbe M,Newhouse EI,Rahim RA,Favier F,Didierjean C,Sousa EH,Stott MB,Dunfield PF,Gonzalez G,Gilles-Gonzalez MA,Najimudin N,Alam M

更新日期：2011-10-20 00:00:00

abstract：:The Fanconi anemia (FA) pathway, of which the FANCD2 protein is a key component, plays crucial roles in the maintenance of hematopoietic stem cells and suppression of carcinogenesis. However, the function of FANCD2 remains unclear. Here, we report that FANCD2 is a novel and specific substrate of caspase 3. Cleavage of...

journal_title：FEBS letters

authors： Sakai W,Sugasawa K

更新日期：2014-10-16 00:00:00

abstract：:The quadruple mutation of the whole basic cluster, K74KKK77 conserved in the catalytic subunits of protein kinase CK2 and implicated in substrate recognition, not only abolishes inhibition by heparin but even induces with some peptide substrates an up to 5-fold stimulation by heparin in the 0.5-5 micrograms/ml concent...

journal_title：FEBS letters

authors： Vaglio P,Sarno S,Marin O,Meggio F,Issinger OG,Pinna LA

更新日期：1996-02-12 00:00:00

abstract：:Sequence comparisons among methionyl-tRNA synthetases from different organisms reveal only one block of homology beyond the last beta strand of the mononucleotide fold. We have introduced a series of semi-conservative amino acid replacements in the conserved motif of yeast methionyl-tRNA synthetase. The results indica...

journal_title：FEBS letters

authors： Despons L,Walter P,Senger B,Ebel JP,Fasiolo F

更新日期：1991-09-09 00:00:00

abstract：:Thioredoxins are a class of small proteins that play a key role in regulating many cellular redox processes. We report here the characterization of the first member of the thioredoxin family in metazoans that is mainly associated with neurons. The Caenorhabditis elegans gene B0228.5 encodes a thioredoxin (TRX-1) that ...

journal_title：FEBS letters

authors： Miranda-Vizuete A,Fierro González JC,Gahmon G,Burghoorn J,Navas P,Swoboda P

更新日期：2006-01-23 00:00:00

abstract：:Amino acid residues 3-15 of mycobacterial HSP60 define a dominant T-cell epitope for HLA-DR3+ve humans and Mamu-DR3+ve rhesus monkeys. Our results show that Mamu-DR3 molecules on PBMC can be efficiently loaded in vivo with the above-mentioned peptides when they are intravenously injected encapsulated in liposomes, but...

journal_title：FEBS letters

authors： t Hart BA,Elferink DG,Drijfhout JW,Storm G,van Blooijs L,Bontrop RE,de Vries RR

更新日期：1997-06-02 00:00:00

abstract：:Blebs of the sarcoplasmic reticulum (SR) membrane of heart muscle cells were generated after saponin perforation of the plasma membrane followed by complete hypercontraction of the cell. Although characteristic proteins of the plasma membrane, namely the beta1-adrenoreceptor and Galphai, were stained by monoclonal ant...

journal_title：FEBS letters

authors： Böhle T,Brandt MC,Henn N,Schmidt A,Bloch W,Beuckelmann DJ

更新日期：2001-09-21 00:00:00

abstract：:The folding of HIV gp41 into a 6-helix bundle drives virus-cell membrane fusion. To examine the structural relationship between the 6-helix bundle core domain and other regions of gp41, we expressed in Escherichia coli, the entire ectodomain of HIV-2(ST) gp41 as a soluble, trimeric maltose-binding protein (MBP)/gp41 c...

journal_title：FEBS letters

authors： Lay CS,Wilson KA,Kobe B,Kemp BE,Drummer HE,Poumbourios P

更新日期：2004-06-04 00:00:00

abstract：:We have studied the modulation and pharmacological properties of two anion channels in T84 cells by recording single channel and transepithelial currents. One channel had an outwardly rectifying current-voltage I/V curve, was rarely active in cell-attached patches, and was unaffected by cAMP. The other channel had low...

journal_title：FEBS letters

authors： Tabcharani JA,Low W,Elie D,Hanrahan JW

更新日期：1990-09-17 00:00:00

abstract：:Proteins and peptides are readily and specifically modified at their sulfhydryl groups by the vapors of a mixture of 4-vinylpyridine and tributylphosphine. The phenylthiohydantoin derivative of S-beta-(4-pyridylethyl)cysteine formed during sequence analysis is easily detectable in current identification systems. ...

journal_title：FEBS letters

authors： Amons R

更新日期：1987-02-09 00:00:00

abstract：:Hic-5/ARA55 is a LIM-only member of the paxillin superfamily. Conflicting reports have suggested that Hic-5/ARA55 can both repress and enhance a number of biological processes, including myogenesis and tumorigenesis. With two Hic-5 isoforms documented, we hypothesized that multiple Hic-5 isoforms may exist that have b...

journal_title：FEBS letters

authors： Gao Z,Schwartz LM

更新日期：2005-10-24 00:00:00

abstract：:Lactase phlorizin hydrolase (LPH; EC 3.2.1.62) is a membrane-bound, family 1 beta-glycosidase found on the brush border of the mammalian small intestine. LPH, purified from sheep small intestine, was capable of hydrolysing a range of flavonol and isoflavone glycosides. The catalytic efficiency (k(cat)/K(m)) for the hy...

journal_title：FEBS letters

authors： Day AJ,Cañada FJ,Díaz JC,Kroon PA,Mclauchlan R,Faulds CB,Plumb GW,Morgan MR,Williamson G

更新日期：2000-02-25 00:00:00

abstract：:Organelles have been studied traditionally as single units, but a novel concept is now emerging: each organelle has distinct functional zones that regulate specific functions. The Golgi apparatus seems to have various zones, including zones for: glycosylphosphatidylinositol-anchored proteins; proteoglycan, mucin and l...

journal_title：FEBS letters

authors： Sasaki K,Yoshida H

更新日期：2019-09-01 00:00:00

abstract：:Many proteins located in the intermembrane space (IMS) of mitochondria are characterized by a low molecular mass, contain highly conserved cysteine residues and coordinate metal ions. Studies on one of these proteins, Tim13, revealed that net translocation across the outer membrane is driven by metal-dependent folding...

journal_title：FEBS letters

authors： Terziyska N,Lutz T,Kozany C,Mokranjac D,Mesecke N,Neupert W,Herrmann JM,Hell K

更新日期：2005-01-03 00:00:00