Mapping the residues of protein kinase CK2 alpha subunit responsible for responsiveness to polyanionic inhibitors.

abstract：:Transglutaminase 2 (TG2) is a GTP-binding protein with transglutaminase activity. Despite advances in the characterization of TG2 functions and their impact on cellular processes, the role of TG2 in Human chronic myelogenous leukemia K562 cell line is still poorly understood. To understand the biological significance ...

journal_title：FEBS letters

authors： Kang SK,Lee JY,Chung TW,Kim CH

更新日期：2004-11-19 00:00:00

abstract：:To demonstrate the interaction of calpastatin (CS) domain L (CSL) with Cav1.2 channel, we investigated the binding of CSL with various C-terminus-derived peptides at≈free, 100 nM, 10 μM, and 1mM Ca(2+) by using the GST pull-down assay method. Besides binding with the IQ motif, CSL was also found to bind with the PreIQ...

journal_title：FEBS letters

authors： Sun W,Feng R,Hu H,Guo F,Gao Q,Shao D,Yin D,Wang H,Sun X,Zhao M,Minobe E,Sun Y,Jiao G,Kameyama M,Hao L

更新日期：2014-03-03 00:00:00

abstract：:The present study provides the first evidence that the abundance of catalytic alpha1-subunit of Na,K-ATPase increases in the course of T cell blast transformation. Immunodepressant cyclosporin A at anti-proliferative doses diminished the induction of alpha1 protein in activated lymphocytes. Furthermore, in competent T...

journal_title：FEBS letters

authors： Marakhova I,Karitskaya I,Aksenov N,Zenin V,Vinogradova T

更新日期：2005-05-23 00:00:00

abstract：:Antipsychotic drugs of known antidiarrhoeal and anticalmodulin activity inhibited the cholera-toxin-catalysed ADP-ribosylation of proteins of Mr 37,000, 40,000 and 45,000 (thought to be regulatory components of the adenylate cyclase complex) that was previously shown to occur in plasma membranes from rabbit intestinal...

journal_title：FEBS letters

authors： Longbottom D,van Heyningen S

更新日期：1990-10-15 00:00:00

abstract：:Using ultrastructural analysis and labeling with polyclonal antibodies that recognize peptide sequences specific for phospholipid binding, we mapped the functional domain structure of intact platelet talin and its proteolytic fragments. The talin dimer, which is crucial for actin and lipid binding, is built of a backb...

journal_title：FEBS letters

authors： Isenberg G,Goldmann WH

更新日期：1998-04-17 00:00:00

abstract：:Two synthetic photoactive azidoarylphosphatidylcholines were used to investigate the level of interaction between D-beta-hydroxybutyrate apodehydrogenase (apoBDH), an amphipathic membrane protein, with the hydrophobic domain of phospholipids. The two synthetic lecithins, PL I (1-myristoyl-2-12-N-(4-azido-2-nitrophenyl...

journal_title：FEBS letters

authors： el Kebbaj MS,Berrez JM,Lakhlifi T,Morpain C,Latruffe N

更新日期：1985-03-11 00:00:00

abstract：:In this study, we demonstrated that laminin-1 (LM-1) induces neurite outgrowth and enhances the expression of neurofilament-L and MAP2 in human bone marrow mesenchymal stem cells (MSCs). The c-Jun transcription factor was strongly activated by LM-1 during neurite induction. Suppression of c-Jun inhibited the expressio...

journal_title：FEBS letters

authors： Mruthyunjaya S,Rumma M,Ravibhushan G,Anjali S,Padma S

更新日期：2011-06-23 00:00:00

abstract：:Using acridine orange as a pH gradient probe, the effects of valinomycin and FCCP on the pH gradient across lysosomal membranes in an ATP-free medium as well as on the rate of the inward ATP-driven proton translocation were investigated. Both lysosome-enriched and highly purified lysosomal preparations from rat liver ...

journal_title：FEBS letters

authors： Dell'Antone P

更新日期：1984-03-12 00:00:00

abstract：:Rats bearing the Yoshida AH-130 ascites hepatoma showed an increased expression of both uncoupling protein-2 (UCP2) (194%) and UCP3 (189%) mRNA levels in skeletal muscle 7 days after tumour inoculation. Interestingly, an even greater increase was observed in mRNA for both UCP2 (278%) and UCP3 (797%) in the pair-fed an...

journal_title：FEBS letters

authors： Sanchís D,Busquets S,Alvarez B,Ricquier D,López-Soriano FJ,Argilés JM

更新日期：1998-10-09 00:00:00

abstract：:We have isolated a 1785-bp complementary DNA (cDNA) encoding the murine P2X7 receptor subunit from NTW8 mouse microglial cells. The encoded protein has 80%, and 85% homology to the human and rat P2X7 subunits, respectively. Functional properties of the heterologously expressed murine P2X7 homomeric receptor broadly re...

journal_title：FEBS letters

authors： Chessell IP,Simon J,Hibell AD,Michel AD,Barnard EA,Humphrey PP

更新日期：1998-11-13 00:00:00

abstract：:Anti-cytokine autoantibodies in healthy individuals have been widely reported but the occurrence is variable and inconstant. We hypothesized that cytokine-binding in vivo may explain their variable and infrequent detection. Therefore, we focused on the detection of the cytokine-autoantibody complexes and found that an...

journal_title：FEBS letters

authors： Watanabe M,Uchida K,Nakagaki K,Kanazawa H,Trapnell BC,Hoshino Y,Kagamu H,Yoshizawa H,Keicho N,Goto H,Nakata K

更新日期：2007-05-15 00:00:00

abstract：:Calcium channels derived from sarcoplasmic reticulum of frog skeletal muscle were fused with planar lipid bilayers. Fractional open times displayed two types of calcium dependence: (i) blockable channels showed a bell-shaped calcium dependence with an activation constant of 4.5 microM, a Hill coefficient for activatio...

journal_title：FEBS letters

authors： Bull R,Marengo JJ

更新日期：1993-10-04 00:00:00

abstract：:Conserved residues of the proteolytic domain of Escherichia coli protease Lon, putative members of the classic catalytic triad (H665, H667, D676, and D743) were identified by comparison of amino acid sequences of Lon proteases. Mutant enzymes containing substitutions D676N, D743N, H665Y, and H667Y were obtained by sit...

journal_title：FEBS letters

authors： Starkova NN,Koroleva EP,Rumsh LD,Ginodman LM,Rotanova TV

更新日期：1998-01-30 00:00:00

abstract：:Factor VIIa is a vitamin K-dependent enzyme whose gamma-carboxyglutamic acid (Gla)-containing domain is important for calcium ion-dependent binding to the cofactor tissue factor and membrane surfaces. This domain contains 10 Gla residues, the individual roles and importance of which are not known. Comparisons with the...

journal_title：FEBS letters

authors： Persson E,Nielsen LS

更新日期：1996-05-06 00:00:00

abstract：:Proteasomes were purified from human hepatoma tissues, and their sensitivities to Na+ and K+ were examined. At concentrations of 10 mM or more, these cations were found to inhibit completely polylysine-activated casein degradation by the purified proteasomes. They also strongly inhibited the hydrolyses of peptides, al...

journal_title：FEBS letters

authors： Seol JH,Park SC,Ha DB,Chung CH,Tanaka K,Ichihara A

更新日期：1989-04-24 00:00:00

abstract：:Adenosine and ATP modulate cellular and tissue functions via specific P1 and P2 receptors, respectively. Although, in general, adenosine inhibits excitability and ATP functions as an excitatory transmitter in the central nervous system, little is known about the direct interaction between P1 and P2 receptors. We recen...

journal_title：FEBS letters

authors： Yoshioka K,Hosoda R,Kuroda Y,Nakata H

更新日期：2002-11-06 00:00:00

abstract：:Fibroblast growth factors (FGFs) and their receptors are expressed in a variety of mammalian tissues, playing a role in development and cell proliferation. While analyzing human sperm motility, we found that sperm treated with endo-β-galactosidase (EBG), which specifically hydrolyzes poly-N-acetyllactosamine type glyc...

journal_title：FEBS letters

authors： Sugihara K,Shibata TK,Takata K,Kimura T,Kanayama N,Williams R,Hatakeyama S,Akama TO,Kuo CW,Khoo KH,Fukuda MN

更新日期：2013-10-01 00:00:00

abstract：:Coronin proteins are widely expressed among eukaryotic organisms. Most coronins consist of a WD-repeat domain followed by a C-terminal coiled coil. Dictyostelium discoideum expresses a single short coronin coronin A, which has been implicated in both actin modulation and multicellular differentiation. Whether coronin ...

journal_title：FEBS letters

authors： Fiedler T,Fabrice TN,Studer V,Vinet A,Faltova L,Kammerer RA,Steinmetz MO,Sharpe T,Pieters J

更新日期：2020-04-16 00:00:00

abstract：:We have identified an Arabidopsis thaliana cDNA, designated as AtTBP1, encoding a protein with a predicted size of 70.6 kDa that specifically binds to the plant telomeric repeat sequence TTTAGGG. AtTBP1 is present as a single-copy gene in Arabidopsis genome and is expressed ubiquitously in various organs. AtTBP1 has a...

journal_title：FEBS letters

authors： Hwang MG,Chung IK,Kang BG,Cho MH

更新日期：2001-08-10 00:00:00

abstract：:The fluorescent calcium indicator 'quin2' was used to demonstrate changes in cytoplasmic calcium concentrations in bovine anterior pituitary cells. The basal calcium concentration was 0.21 +/- 0.02 microM (mean of 4 cell preparations). Thyroliberin (TRH) (10(-10) - 10(-6) M) rapidly and at the higher concentrations tr...

journal_title：FEBS letters

authors： Schofield JG

更新日期：1983-08-08 00:00:00

abstract：:Protein gene product 9.5 (PGP 9.5), which in the normal nervous system is restricted to certain neurons, has been detected in two glioma cell lines, rat C6 and human GL15, by immunoblotting and immunocytochemistry. Its expression in these cells depends on the cellular growth state, being maximal between the first and ...

journal_title：FEBS letters

authors： Giambanco I,Bianchi R,Ceccarelli P,Pula G,Sorci G,Antonioli S,Bocchini V,Donato R

更新日期：1991-09-23 00:00:00

abstract：:Syndecans are transmembrane heparan sulfate proteoglycans, with roles in development, tumorigenesis and inflammation, and growing evidence for involvement in tissue regeneration. This is a fast developing field with the prospect of utilizing tissue engineering and biomaterials in novel therapies. Syndecan receptors ar...

journal_title：FEBS letters

authors： Chung H,Multhaupt HA,Oh ES,Couchman JR

更新日期：2016-08-01 00:00:00

abstract：:Germ-line mutations in BRCA1 are associated with an increased lifetime risk of developing breast and/or ovarian tumors. The BRCA1 gene product is a 220-kDa protein that contains a tandem of two BRCA1 C-terminal (BRCT) domains required for transcription. In an attempt to understand how BRCA1 exerts its function through...

journal_title：FEBS letters

authors： Yan J,Zhu J,Zhong H,Lu Q,Huang C,Ye Q

更新日期：2003-10-09 00:00:00

abstract：:BSP-30K is a major acidic glycoprotein of bovine seminal plasma. It displays heparin-, gelatin-, and phospholipid-binding activities. BSP-30K binds to spermatozoa upon ejaculation and is thought to play a role in sperm capacitation. We have determined its amino acid sequence, disulfide bonds, and 0-glycosylation sites...

journal_title：FEBS letters

authors： Calvete JJ,Mann K,Sanz L,Raida M,Töpfer-Petersen E

更新日期：1996-12-09 00:00:00

abstract：:F-box proteins play pivotal roles in multiple cellular processes; however, little is known about their functions in glioma progression. In this study, we found that expression of the F-box and leucine-rich repeat protein 18 (FBXL18) is significantly upregulated in glioma tissues. Depletion of FBXL18 in glioma cells su...

journal_title：FEBS letters

authors： Zhang J,Yang Z,Ou J,Xia X,Zhi F,Cui J

更新日期：2017-01-01 00:00:00

abstract：:To complete the amino acid sequence of GTP:AMP phosphotransferase (MgGTP + AMP in equilibrium with MgGDP + ADP) from beef heart mitochondria it was necessary to sequence an intermediate region of about 33 residues after position 102 [(1984) Eur. J. Biochem. 143, 331-339] and find a suitable overlap with the rest of th...

journal_title：FEBS letters

authors： Tomasselli AG,Frank R,Schiltz E

更新日期：1986-07-07 00:00:00

abstract：:The receptor structure of Clostridium botulinum neurotoxin type A was analysed by TLC immunostaining. GQ1b was found to be the most potent receptor, and the neurotoxin also bound to GT1b and GD1a, but not to GM3, GM2, GM1, GD3, GD1b and GT1a. Optimum binding of neurotoxin to the ganglioside appeared in 0.01 M phosphat...

journal_title：FEBS letters

authors： Takamizawa K,Iwamori M,Kozaki S,Sakaguchi G,Tanaka R,Takayama H,Nagai Y

更新日期：1986-06-09 00:00:00

abstract：:Leishmania internalize hemoglobin (Hb) via a specific receptor (HbR) for their survival. To identify the Hb-binding domain of HbR, we cloned and expressed several truncated proteins of HbR and determined their ability to bind Hb. Our findings reveal that 90% of Hb-binding activity is retained in HbR41-80 in comparison...

journal_title：FEBS letters

authors： Rastogi R,Verma JK,Singh V,Krishnamurthy G,Sood C,Kapoor A,Kumar K,Ansari I,Mukhopadhyay A

更新日期：2020-12-14 00:00:00

abstract：:The ubiquitin-editing enzyme A20 suppresses nuclear factor-κB (NF-κB) activation and tumor necrosis factor-α (TNF-α)-induced apoptosis in a deubiquitinating and ubiquitin ligase activity-dependent manner. Although recent studies revealed that A20 regulates NF-κB independently of its enzymatic activity through its seve...

journal_title：FEBS letters

authors： Yamaguchi N,Yamaguchi N

更新日期：2015-05-22 00:00:00

abstract：:APE-independent base excision repair (BER) pathway plays an important role in the regulation of DNA repair mechanisms. In this study it has been found that recently discovered tyrosyl-DNA phosphodiesterase 1 (Tdp1) catalyzes the AP site cleavage reaction to generate breaks with the 3'- and 5'-phosphate termini. The re...

journal_title：FEBS letters

authors： Lebedeva NA,Rechkunova NI,Lavrik OI

更新日期：2011-02-18 00:00:00