Mapping the residues of protein kinase CK2 alpha subunit responsible for responsiveness to polyanionic inhibitors.


:The quadruple mutation of the whole basic cluster, K74KKK77 conserved in the catalytic subunits of protein kinase CK2 and implicated in substrate recognition, not only abolishes inhibition by heparin but even induces with some peptide substrates an up to 5-fold stimulation by heparin in the 0.5-5 micrograms/ml concentration range. Two other mutants defective in substrate recognition, R191, 195K198A and K79R80K83A, display either a 100-fold reduction or no alteration at all in heparin inhibition, respectively. In contrast sensitivity to heparin inhibition is increased 30-fold by a single mutation affecting Arg-228 while it is not altered by a triple mutation in the small insert of subdomain XI (mutant R278K279R280A). The effect of the same mutations on inhibition by pseudosubstrate EEEEEYEEEEEEE is different, the mutant displaying the most reduced sensitivity being R191,195K198A, followed by K74-77A and K79R80K83A; the other mutants are almost indistinguishable from CK2 wild type. Substantial reduction of inhibition by poly(Glu,Tyr)4:1 is only observable with mutant R191,195K198A, whereas R228A is significantly more sensitive to inhibition. These data show that the mode of inhibition of CK2 by polyanionic compounds occurs through substantially different mechanisms involving residues that are variably concerned with substrate recognition.




FEBS letters


Vaglio P,Sarno S,Marin O,Meggio F,Issinger OG,Pinna LA




Has Abstract


1996-02-12 00:00:00














  • Cytokinin affinity purification and identification of a tobacco BY-2 adenosine kinase.

    abstract::Adenosine kinase is one of the enzymes potentially responsible for the formation of cytokinin nucleotides in plants. Using a zeatin affinity column a 40 kDa protein was isolated from tobacco Bright Yellow 2 (TBY-2) and identified by mass spectrometry as adenosine kinase. The ligand interaction reported here can be dis...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Laukens K,Lenobel R,Strnad M,Van Onckelen H,Witters E

    更新日期:2003-01-02 00:00:00

  • A stability transition at mildly acidic pH in the alpha-hemolysin (alpha-toxin) from Staphylococcus aureus.

    abstract::The effects of mildly acidic conditions on the free energy of unfolding (DeltaG(u)(buff)) of the pore-forming alpha-hemolysin (alphaHL) from Staphylococcus aureus were assessed between pH 5.0 and 7.5 by measuring intrinsic tryptophan fluorescence, circular dichroism and elution time in size exclusion chromatography du...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bortoleto RK,Ward RJ

    更新日期:1999-10-15 00:00:00

  • Measurement of GTP gamma S binding to specific G proteins in membranes using G-protein antibodies.

    abstract::We developed a novel method to quantitatively measure GTP gamma S binding to specific G proteins in crude membranes using G-protein antibodies. The basic strategy was that the materials were initially incubated with [35S]GTP gamma S at 37 degrees C. After 4 degrees C incubation in the wells of an ELISA plate precoated...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Okamoto T,Ikezu T,Murayama Y,Ogata E,Nishimoto I

    更新日期:1992-06-29 00:00:00

  • AID recruits the RNA exosome to degrade HIV-1 nascent transcripts through interaction with the Tat-P-TEFb-TAR RNP complex.

    abstract::Activation-induced cytidine deaminase (AID), a member of the APOBEC family that induces antibody diversification, has been shown to inhibit the replication of hepatitis B virus, Kaposi's sarcoma-associated herpesvirus, and retro-transposons. However, whether AID can inhibit human immunodeficiency virus 1 (HIV-1) repli...

    journal_title:FEBS letters

    pub_type: 信件


    authors: Wang R,Zhang X,Ding H,Qiao Y,Han X,Geng W,Guan G,Cui H,Zhao B,Wu Y,Liang G,Shang H

    更新日期:2018-01-01 00:00:00

  • Selective inhibition of divergent seryl-tRNA synthetases by serine analogues.

    abstract::Seryl-tRNA synthetases (SerRSs) fall into two distinct evolutionary groups of enzymes, bacterial and methanogenic. These two types of SerRSs display only minimal sequence similarity, primarily within the class II conserved motifs, and possess distinct modes of tRNA(Ser) recognition. In order to determine whether the t...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ahel D,Slade D,Mocibob M,Söll D,Weygand-Durasevic I

    更新日期:2005-08-15 00:00:00

  • Feedback regulation of Ras2 guanine nucleotide exchange factor (Ras2-GEF) activity of Cdc25p by Cdc25p phosphorylation in the yeast Saccharomyces cerevisiae.

    abstract::Ras-GEF Cdc25p has been found to be hyperphosphorylated upon glucose addition. This work provides evidence indicating that PKA activity positively regulates the degree of Cdc25p phosphorylation, and that the intracellular association of Cdc25p and Ras2p is independent of PKA activity. In vitro experiments revealed tha...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Jian D,Aili Z,Xiaojia B,Huansheng Z,Yun H

    更新日期:2010-12-01 00:00:00

  • Ascorbic acid can either increase or decrease low density lipoprotein modification.

    abstract::In freshly prepared low density lipoprotein (LDL), ascorbate inhibited LDL oxidation by macrophages at the higher concentrations tested (60-100 microM). In contrast, with LDL that had been allowed to autoxidise in the refrigerator (3 degrees C) for at least 10 weeks after isolation (mildly oxidised or minimally-modifi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Stait SE,Leake DS

    更新日期:1994-03-21 00:00:00

  • The refined 2.3 A crystal structure of human leukocyte elastase in a complex with a valine chloromethyl ketone inhibitor.

    abstract::The stoichiometric complex formed between human leukocyte elastase and a synthetic MeO-Suc-Ala-Ala-Pro-Val chloromethyl ketone inhibitor was co-crystallized and its X-ray structure determined, using Patterson search methods. Its structure has been crystallographically refined to a final R value of 0.145 (8.0 and 2.3 A...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wei AZ,Mayr I,Bode W

    更新日期:1988-07-18 00:00:00

  • Complex demethylation patterns at Sp1 binding sites in F9 embryonal carcinoma cells.

    abstract::The ubiquitous transcription factor Sp1 has been implicated in the mechanism which maintains CpG islands methylation-free. Plasmids containing GC boxes (Sp1 sites) were in vitro methylated at every CpG dinucleotide. After stable introduction into F9 embryonal carcinoma cells, we analysed the methylation of the sequenc...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Silke J,Rother KI,Georgiev O,Schaffner W,Matsuo K

    更新日期:1995-08-21 00:00:00

  • The expression of the H19 gene and its function in human bladder carcinoma cell lines.

    abstract::The human H19 gene is a paternally imprinted oncofetal gene, highly expressed in several fetal tissues, down-regulated in nearly all adult tissues but re-expressed in carcinomas of tissues which express the gene in fetal life. It has no known protein product and till today, no function could be designated to H19 RNA. ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ohana P,Kopf E,Bibi O,Ayesh S,Schneider T,Laster M,Tykocinski M,de Groot N,Hochberg A

    更新日期:1999-07-02 00:00:00

  • Role of insulin receptor substrate-2 in interleukin-9-dependent proliferation.

    abstract::Interleukin-9 (IL-9) stimulation results in JAK, STAT and IRS1/2 phosphorylation. The role of IRS adaptor proteins in IL-9 signaling is not clear. We show that IL-9 induces IRS2 phosphorylation and association with phosphatidylinositol-3 kinase (PI 3-K) p85 subunit in TS1 cells and BaF/9R cells, which proliferate upon...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Demoulin JB,Grasso L,Atkins JM,Stevens M,Louahed J,Levitt RC,Nicolaides NC,Renauld JC

    更新日期:2000-10-06 00:00:00

  • The His-probe method: effects of histidine residues introduced into the complementarity-determining regions of antibodies on antigen-antibody interactions at different pH values.

    abstract::We examined the effects of histidine residues that were artificially introduced into complementarity-determining regions of antibodies on antigen-antibody interactions at different pH values. Using a monoclonal antibody specific for hen egg-white lysozyme and three mutant antibodies that contained a histidine residue,...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ito W,Sakato N,Fujio H,Yutani K,Arata Y,Kurosawa Y

    更新日期:1992-08-31 00:00:00

  • Galanin reduces PDBu-induced protein phosphorylation in rat ventral hippocampus.

    abstract::The effect of galanin (GAL) on basal and phorbol-12,13-dibutyrate (PDBu) induced protein phosphorylation in rat ventral hippocampal miniprisms was investigated. GAL (0.5, 1 and 2 microM) inhibited PDBu stimulation in a concentration-dependent manner without altering basal protein phosphorylation. This inhibitory effec...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: La Porta C,Bianchi R,Sozzani S,Bartfai T,Consolo S

    更新日期:1992-03-23 00:00:00

  • Capsular polysaccharide production and serum survival of Vibrio vulnificus are dependent on antitermination control by RfaH.

    abstract::The human pathogen Vibrio vulnificus undergoes phase variation among colonial morphotypes, including a virulent opaque form which produces capsular polysaccharide (CPS) and a translucent phenotype that produces little or no CPS and is attenuated. Here, we found that a V. vulnificus mutant defective for RfaH antitermin...

    journal_title:FEBS letters

    pub_type: 信件


    authors: Garrett SB,Garrison-Schilling KL,Cooke JT,Pettis GS

    更新日期:2016-12-01 00:00:00

  • The interaction of amino-deuteromethylated melittin with phospholipid membranes studied by deuterium NMR.

    abstract::Melittin, deuteromethylated on each of the four amino groups (Gly-1 N alpha and Lys-7, 21, and 23 N epsilon), was prepared by reductive methylation using deuteroformaldehyde and NaBD3CN. Deuterium NMR spectra were obtained for the modified peptide (D-melittin) bound to phospholipid bilayers and erythrocyte ghosts. D-M...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Dempsey CE,Cryer GD,Watts A

    更新日期:1987-06-22 00:00:00

  • In vivo transfer of hepatocyte growth factor gene accelerates proliferation of hepatic oval cells in a 2-acetylaminofluorene/partial hepatectomy model in rats.

    abstract::To clarify the effect of hepatocyte growth factor (HGF) on proliferation of hepatic oval cells, we transferred HGF gene into liver of the Solt-Farber rat model. Male Fisher 344 rats were infected with a recombinant adenovirus carrying the cDNA for HGF (pAxCAHGF) from tail vein. HGF mRNA showed its peak at 4 days, and ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Shiota G,Kunisada T,Oyama K,Udagawa A,Nomi T,Tanaka K,Tsutsumi A,Isono M,Nakamura T,Hamada H,Sakatani T,Sell S,Sato K,Ito H,Kawasaki H

    更新日期:2000-03-31 00:00:00

  • Metabolic and signaling properties of an Itpk gene family in Glycine max.

    abstract::We have cloned and characterized four Itpk genes from soybean. All four recombinant Itpk proteins showed canonical Ins(1,3,4)P3 5/6-kinase activity, but a kinetic analysis raised questions about its biological significance. Instead, we provide evidence that one alternative biological role for soybean Itpks is to inter...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Stiles AR,Qian X,Shears SB,Grabau EA

    更新日期:2008-06-11 00:00:00

  • Autophosphorylation of the two C-terminal tyrosine residues Tyr1316 and Tyr1322 modulates the activity of the insulin receptor kinase in vitro.

    abstract::Previously, several studies have demonstrated that autophosphorylation of the C-terminal tyrosine residues (Tyr1316 and Tyr1322) affects the signaling properties of the insulin receptor in vivo. To assess the biochemical consequences of the C-terminal phosphorylation in vitro, we have constructed, purified and charact...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tennagels N,Bergschneider E,Al-Hasani H,Klein HW

    更新日期:2000-08-11 00:00:00

  • Perspectives on the automatic design of regulatory systems for synthetic biology.

    abstract::Automatic design is based on computational modeling and optimization methods to provide prototype designs to targeted problems in an unsupervised manner. For biological circuits, we need to produce quantitative predictions of cell behavior for a given genotype as consequence of the different molecular interactions. Au...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Rodrigo G,Carrera J,Landrain TE,Jaramillo A

    更新日期:2012-07-16 00:00:00

  • Light-induced stimulation of carbonic anhydrase activity in pea thylakoids.

    abstract::Stimulation of the bicarbonate dehydration reaction in thylakoid suspension under conditions of saturating light at pH 7.6-8.0 was discovered. This effect was inhibited by nigericin or the lipophilic carbonic anhydrase (CA) inhibitor ethoxyzolamide (EZ), but not by the hydrophilic CA inhibitor, acetazolamide. It was s...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Moskvin OV,Ivanov BN,Ignatova LK,Kollmeier MA

    更新日期:2000-03-31 00:00:00

  • Replicable and recombinogenic RNAs.

    abstract::This paper summarizes results of the 40-year studies on replication and recombination of RNA molecules in the cell-free amplification system of bacteriophage Q. Special attention is paid to the molecular colony technique that has provided for the discovery of the nature of "spontaneous" RNA synthesis by Q replicase an...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Chetverin AB

    更新日期:2004-06-01 00:00:00

  • 2-Arachidonoylglycerol, an endogenous cannabinoid receptor agonist: identification as one of the major species of monoacylglycerols in various rat tissues, and evidence for its generation through CA2+-dependent and -independent mechanisms.

    abstract::The molecular species compositions of monoacylglycerols obtained from various rat tissues were examined by reverse-phase high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS) analyses. We confirmed that 2-arachidonoylglycerol, an endogenous cannabinoid receptor agonist, is one ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kondo S,Kondo H,Nakane S,Kodaka T,Tokumura A,Waku K,Sugiura T

    更新日期:1998-06-12 00:00:00

  • Prediction of antigenic determinants and secondary structures of the major AIDS virus proteins.

    abstract::Criteria for the design of peptide vaccines to prevent AIDS are presented. The best vaccine candidates contain both B and T lymphocyte-defined epitopes in regions conserved in sequence between viral isolates. We propose that attention should focus on proteins specified by the gag and, possibly, pol genes in addition t...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sternberg MJ,Barton GJ,Zvelebil MJ,Cookson J,Coates AR

    更新日期:1987-06-29 00:00:00

  • Melatonin prevents changes in microsomal membrane fluidity during induced lipid peroxidation.

    abstract::We tested the effect of melatonin on membrane fluidity in microsomes of a rat liver model in which lipid peroxidation was induced by the addition of FeCl3, ADP and NADPH. Membrane fluidity was monitored using fluorescence spectroscopy and lipid peroxidation was estimated by quantifying malonaldehyde (MDA)+4-hydroxyalk...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: García JJ,Reiter RJ,Guerrero JM,Escames G,Yu BP,Oh CS,Muñoz-Hoyos A

    更新日期:1997-05-26 00:00:00

  • Investigation of H2AX methylation by the SUV39H2 protein lysine methyltransferase.

    abstract::The H3K9 protein lysine methyltransferase SUV39H2 was reported to methylate K134 of H2AX and stimulate H2AX phosphorylation during DNA damage response [Sone K et al. (2014) Nat Commun 5, 5691]. However, the sequence context of H2AX-K134 differs from the specificity of SUV39H2. We performed in vitro methylation reactio...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Schuhmacher MK,Kudithipudi S,Jeltsch A

    更新日期:2016-06-01 00:00:00

  • A novel fluorescent marker for assembled mitochondria ATP synthase of yeast. OSCP subunit fused to green fluorescent protein is assembled into the complex in vivo.

    abstract::We have shown that OSCP, a subunit of yeast mitochondrial ATP synthase, can be incorporated into the intact enzyme as a fusion protein representing OSCP fused at its C-terminus to the green fluorescent protein (GFP) of Aequorea victoria. The relevant fusion OSCP-GFP-h6 additionally contains a hexahistidine tag at the ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Prescott M,Lourbakos A,Bateson M,Boyle G,Nagley P,Devenish RJ

    更新日期:1997-07-07 00:00:00

  • Six myosin heavy chain isoforms are expressed during chick breast muscle development.

    abstract::Two major embryonic myosin heavy chains are expressed in embryonic chick breast muscle until the first week after hatching. Of these, one is already detected in the 8-day-old embryo. The other appears on day 12. Two putative slow embryonic isoforms represent minor components transiently expressed between days 8 and 12...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hofmann S,Düsterhöft S,Pette D

    更新日期:1988-10-10 00:00:00

  • Beta-glucosidase activity towards a bile acid glucoside in human liver.

    abstract::Human liver contains hydrolytic activity toward 3 beta-glucosido-chenodeoxycholic acid. This beta-glucosidase activity, localized predominantly in the microsomal fraction, was optimally active in the presence of divalent metal ions close to pH 5.0 and was inhibited by EDTA. Kinetic parameters and other catalytic prope...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Matern H,Gartzen R,Matern S

    更新日期:1992-12-14 00:00:00

  • Effect of starvation and insulin in vivo on the activity of the pyruvate dehydrogenase complex in rat skeletal muscles.

    abstract::The in vivo responses of pyruvate dehydrogenase (PDH) complex to starvation and insulin was assessed in heart, diaphragm and red quadriceps muscle. PDH complex activity was decreased by starvation (3.4-10.2-fold), the magnitude of change depending on muscle type. Insulin increased PDH activity in all muscle types. In ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Denyer GS,Lam D,Cooney GJ,Caterson ID

    更新日期:1989-07-03 00:00:00

  • Reduced maximum capacity of glycolysis in brown adipose tissue of genetically obese, diabetic (db/db) mice and its restoration following treatment with a thermogenic beta-adrenoceptor agonist.

    abstract::The maximal activities of the key glycolytic enzymes hexokinase and 6-phosphofructokinase, were reduced in brown adipose tissue in db/db mice compared to their lean littermates. Treatment of db/db mice with the thermogenic beta-adrenoceptor agonist, BRL 26830, restored normoglycaemia. The only significant increase in ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Young P,Cawthorne MA,Levy AL,Wilson K

    更新日期:1984-10-15 00:00:00