Mapping the residues of protein kinase CK2 alpha subunit responsible for responsiveness to polyanionic inhibitors.


:The quadruple mutation of the whole basic cluster, K74KKK77 conserved in the catalytic subunits of protein kinase CK2 and implicated in substrate recognition, not only abolishes inhibition by heparin but even induces with some peptide substrates an up to 5-fold stimulation by heparin in the 0.5-5 micrograms/ml concentration range. Two other mutants defective in substrate recognition, R191, 195K198A and K79R80K83A, display either a 100-fold reduction or no alteration at all in heparin inhibition, respectively. In contrast sensitivity to heparin inhibition is increased 30-fold by a single mutation affecting Arg-228 while it is not altered by a triple mutation in the small insert of subdomain XI (mutant R278K279R280A). The effect of the same mutations on inhibition by pseudosubstrate EEEEEYEEEEEEE is different, the mutant displaying the most reduced sensitivity being R191,195K198A, followed by K74-77A and K79R80K83A; the other mutants are almost indistinguishable from CK2 wild type. Substantial reduction of inhibition by poly(Glu,Tyr)4:1 is only observable with mutant R191,195K198A, whereas R228A is significantly more sensitive to inhibition. These data show that the mode of inhibition of CK2 by polyanionic compounds occurs through substantially different mechanisms involving residues that are variably concerned with substrate recognition.




FEBS letters


Vaglio P,Sarno S,Marin O,Meggio F,Issinger OG,Pinna LA




Has Abstract


1996-02-12 00:00:00














  • Overexpression of transglutaminase 2 accelerates the erythroid differentiation of human chronic myelogenous leukemia K562 cell line through PI3K/Akt signaling pathway.

    abstract::Transglutaminase 2 (TG2) is a GTP-binding protein with transglutaminase activity. Despite advances in the characterization of TG2 functions and their impact on cellular processes, the role of TG2 in Human chronic myelogenous leukemia K562 cell line is still poorly understood. To understand the biological significance ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kang SK,Lee JY,Chung TW,Kim CH

    更新日期:2004-11-19 00:00:00

  • The Ca(2+)-dependent interaction of calpastatin domain L with the C-terminal tail of the Cav1.2 channel.

    abstract::To demonstrate the interaction of calpastatin (CS) domain L (CSL) with Cav1.2 channel, we investigated the binding of CSL with various C-terminus-derived peptides at≈free, 100 nM, 10 μM, and 1mM Ca(2+) by using the GST pull-down assay method. Besides binding with the IQ motif, CSL was also found to bind with the PreIQ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sun W,Feng R,Hu H,Guo F,Gao Q,Shao D,Yin D,Wang H,Sun X,Zhao M,Minobe E,Sun Y,Jiao G,Kameyama M,Hao L

    更新日期:2014-03-03 00:00:00

  • Interleukin-2-dependent regulation of Na/K pump in human lymphocytes.

    abstract::The present study provides the first evidence that the abundance of catalytic alpha1-subunit of Na,K-ATPase increases in the course of T cell blast transformation. Immunodepressant cyclosporin A at anti-proliferative doses diminished the induction of alpha1 protein in activated lymphocytes. Furthermore, in competent T...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Marakhova I,Karitskaya I,Aksenov N,Zenin V,Vinogradova T

    更新日期:2005-05-23 00:00:00

  • Effect of antipsychotic drugs on the molecular action of cholera toxin in rabbit intestinal epithelial cells.

    abstract::Antipsychotic drugs of known antidiarrhoeal and anticalmodulin activity inhibited the cholera-toxin-catalysed ADP-ribosylation of proteins of Mr 37,000, 40,000 and 45,000 (thought to be regulatory components of the adenylate cyclase complex) that was previously shown to occur in plasma membranes from rabbit intestinal...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Longbottom D,van Heyningen S

    更新日期:1990-10-15 00:00:00

  • Peptide-specific antibodies localize the major lipid binding sites of talin dimers to oppositely arranged N-terminal 47 kDa subdomains.

    abstract::Using ultrastructural analysis and labeling with polyclonal antibodies that recognize peptide sequences specific for phospholipid binding, we mapped the functional domain structure of intact platelet talin and its proteolytic fragments. The talin dimer, which is crucial for actin and lipid binding, is built of a backb...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Isenberg G,Goldmann WH

    更新日期:1998-04-17 00:00:00

  • Photolabelling of D-beta-hydroxybutyrate apodehydrogenase with azidoaryl phospholipids.

    abstract::Two synthetic photoactive azidoarylphosphatidylcholines were used to investigate the level of interaction between D-beta-hydroxybutyrate apodehydrogenase (apoBDH), an amphipathic membrane protein, with the hydrophobic domain of phospholipids. The two synthetic lecithins, PL I (1-myristoyl-2-12-N-(4-azido-2-nitrophenyl...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: el Kebbaj MS,Berrez JM,Lakhlifi T,Morpain C,Latruffe N

    更新日期:1985-03-11 00:00:00

  • c-Jun/AP-1 transcription factor regulates laminin-1-induced neurite outgrowth in human bone marrow mesenchymal stem cells: role of multiple signaling pathways.

    abstract::In this study, we demonstrated that laminin-1 (LM-1) induces neurite outgrowth and enhances the expression of neurofilament-L and MAP2 in human bone marrow mesenchymal stem cells (MSCs). The c-Jun transcription factor was strongly activated by LM-1 during neurite induction. Suppression of c-Jun inhibited the expressio...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Mruthyunjaya S,Rumma M,Ravibhushan G,Anjali S,Padma S

    更新日期:2011-06-23 00:00:00

  • Electrogenicity of the lysosomal proton pump.

    abstract::Using acridine orange as a pH gradient probe, the effects of valinomycin and FCCP on the pH gradient across lysosomal membranes in an ATP-free medium as well as on the rate of the inward ATP-driven proton translocation were investigated. Both lysosome-enriched and highly purified lysosomal preparations from rat liver ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Dell'Antone P

    更新日期:1984-03-12 00:00:00

  • Skeletal muscle UCP2 and UCP3 gene expression in a rat cancer cachexia model.

    abstract::Rats bearing the Yoshida AH-130 ascites hepatoma showed an increased expression of both uncoupling protein-2 (UCP2) (194%) and UCP3 (189%) mRNA levels in skeletal muscle 7 days after tumour inoculation. Interestingly, an even greater increase was observed in mRNA for both UCP2 (278%) and UCP3 (797%) in the pair-fed an...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sanchís D,Busquets S,Alvarez B,Ricquier D,López-Soriano FJ,Argilés JM

    更新日期:1998-10-09 00:00:00

  • Cloning and functional characterisation of the mouse P2X7 receptor.

    abstract::We have isolated a 1785-bp complementary DNA (cDNA) encoding the murine P2X7 receptor subunit from NTW8 mouse microglial cells. The encoded protein has 80%, and 85% homology to the human and rat P2X7 subunits, respectively. Functional properties of the heterologously expressed murine P2X7 homomeric receptor broadly re...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Chessell IP,Simon J,Hibell AD,Michel AD,Barnard EA,Humphrey PP

    更新日期:1998-11-13 00:00:00

  • Anti-cytokine autoantibodies are ubiquitous in healthy individuals.

    abstract::Anti-cytokine autoantibodies in healthy individuals have been widely reported but the occurrence is variable and inconstant. We hypothesized that cytokine-binding in vivo may explain their variable and infrequent detection. Therefore, we focused on the detection of the cytokine-autoantibody complexes and found that an...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Watanabe M,Uchida K,Nakagaki K,Kanazawa H,Trapnell BC,Hoshino Y,Kagamu H,Yoshizawa H,Keicho N,Goto H,Nakata K

    更新日期:2007-05-15 00:00:00

  • Sarcoplasmic reticulum release channels from frog skeletal muscle display two types of calcium dependence.

    abstract::Calcium channels derived from sarcoplasmic reticulum of frog skeletal muscle were fused with planar lipid bilayers. Fractional open times displayed two types of calcium dependence: (i) blockable channels showed a bell-shaped calcium dependence with an activation constant of 4.5 microM, a Hill coefficient for activatio...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bull R,Marengo JJ

    更新日期:1993-10-04 00:00:00

  • Mutations in the proteolytic domain of Escherichia coli protease Lon impair the ATPase activity of the enzyme.

    abstract::Conserved residues of the proteolytic domain of Escherichia coli protease Lon, putative members of the classic catalytic triad (H665, H667, D676, and D743) were identified by comparison of amino acid sequences of Lon proteases. Mutant enzymes containing substitutions D676N, D743N, H665Y, and H667Y were obtained by sit...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Starkova NN,Koroleva EP,Rumsh LD,Ginodman LM,Rotanova TV

    更新日期:1998-01-30 00:00:00

  • Site-directed mutagenesis but not gamma-carboxylation of Glu-35 in factor VIIa affects the association with tissue factor.

    abstract::Factor VIIa is a vitamin K-dependent enzyme whose gamma-carboxyglutamic acid (Gla)-containing domain is important for calcium ion-dependent binding to the cofactor tissue factor and membrane surfaces. This domain contains 10 Gla residues, the individual roles and importance of which are not known. Comparisons with the...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Persson E,Nielsen LS

    更新日期:1996-05-06 00:00:00

  • Na+, K+-specific inhibition of protein and peptide hydrolyses by proteasomes from human hepatoma tissues.

    abstract::Proteasomes were purified from human hepatoma tissues, and their sensitivities to Na+ and K+ were examined. At concentrations of 10 mM or more, these cations were found to inhibit completely polylysine-activated casein degradation by the purified proteasomes. They also strongly inhibited the hydrolyses of peptides, al...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Seol JH,Park SC,Ha DB,Chung CH,Tanaka K,Ichihara A

    更新日期:1989-04-24 00:00:00

  • Hetero-oligomerization of adenosine A1 receptors with P2Y1 receptors in rat brains.

    abstract::Adenosine and ATP modulate cellular and tissue functions via specific P1 and P2 receptors, respectively. Although, in general, adenosine inhibits excitability and ATP functions as an excitatory transmitter in the central nervous system, little is known about the direct interaction between P1 and P2 receptors. We recen...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Yoshioka K,Hosoda R,Kuroda Y,Nakata H

    更新日期:2002-11-06 00:00:00

  • Attenuation of fibroblast growth factor signaling by poly-N-acetyllactosamine type glycans.

    abstract::Fibroblast growth factors (FGFs) and their receptors are expressed in a variety of mammalian tissues, playing a role in development and cell proliferation. While analyzing human sperm motility, we found that sperm treated with endo-β-galactosidase (EBG), which specifically hydrolyzes poly-N-acetyllactosamine type glyc...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sugihara K,Shibata TK,Takata K,Kimura T,Kanayama N,Williams R,Hatakeyama S,Akama TO,Kuo CW,Khoo KH,Fukuda MN

    更新日期:2013-10-01 00:00:00

  • Homodimerization of coronin A through the C-terminal coiled-coil domain is essential for multicellular differentiation of Dictyostelium discoideum.

    abstract::Coronin proteins are widely expressed among eukaryotic organisms. Most coronins consist of a WD-repeat domain followed by a C-terminal coiled coil. Dictyostelium discoideum expresses a single short coronin coronin A, which has been implicated in both actin modulation and multicellular differentiation. Whether coronin ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Fiedler T,Fabrice TN,Studer V,Vinet A,Faltova L,Kammerer RA,Steinmetz MO,Sharpe T,Pieters J

    更新日期:2020-04-16 00:00:00

  • Sequence-specific binding property of Arabidopsis thaliana telomeric DNA binding protein 1 (AtTBP1).

    abstract::We have identified an Arabidopsis thaliana cDNA, designated as AtTBP1, encoding a protein with a predicted size of 70.6 kDa that specifically binds to the plant telomeric repeat sequence TTTAGGG. AtTBP1 is present as a single-copy gene in Arabidopsis genome and is expressed ubiquitously in various organs. AtTBP1 has a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hwang MG,Chung IK,Kang BG,Cho MH

    更新日期:2001-08-10 00:00:00

  • Use of a trapped fluorescent indicator to demonstrate effects of thyroliberin and dopamine on cytoplasmic calcium concentrations in bovine anterior pituitary cells.

    abstract::The fluorescent calcium indicator 'quin2' was used to demonstrate changes in cytoplasmic calcium concentrations in bovine anterior pituitary cells. The basal calcium concentration was 0.21 +/- 0.02 microM (mean of 4 cell preparations). Thyroliberin (TRH) (10(-10) - 10(-6) M) rapidly and at the higher concentrations tr...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Schofield JG

    更新日期:1983-08-08 00:00:00

  • 'Neuron-specific' protein gene product 9.5 (PGP 9.5) is also expressed in glioma cell lines and its expression depends on cellular growth state.

    abstract::Protein gene product 9.5 (PGP 9.5), which in the normal nervous system is restricted to certain neurons, has been detected in two glioma cell lines, rat C6 and human GL15, by immunoblotting and immunocytochemistry. Its expression in these cells depends on the cellular growth state, being maximal between the first and ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Giambanco I,Bianchi R,Ceccarelli P,Pula G,Sorci G,Antonioli S,Bocchini V,Donato R

    更新日期:1991-09-23 00:00:00

  • Minireview: Syndecans and their crucial roles during tissue regeneration.

    abstract::Syndecans are transmembrane heparan sulfate proteoglycans, with roles in development, tumorigenesis and inflammation, and growing evidence for involvement in tissue regeneration. This is a fast developing field with the prospect of utilizing tissue engineering and biomaterials in novel therapies. Syndecan receptors ar...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Chung H,Multhaupt HA,Oh ES,Couchman JR

    更新日期:2016-08-01 00:00:00

  • BRCA1 interacts with FHL2 and enhances FHL2 transactivation function.

    abstract::Germ-line mutations in BRCA1 are associated with an increased lifetime risk of developing breast and/or ovarian tumors. The BRCA1 gene product is a 220-kDa protein that contains a tandem of two BRCA1 C-terminal (BRCT) domains required for transcription. In an attempt to understand how BRCA1 exerts its function through...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Yan J,Zhu J,Zhong H,Lu Q,Huang C,Ye Q

    更新日期:2003-10-09 00:00:00

  • The primary structure of BSP-30K, a major lipid-, gelatin-, and heparin-binding glycoprotein of bovine seminal plasma.

    abstract::BSP-30K is a major acidic glycoprotein of bovine seminal plasma. It displays heparin-, gelatin-, and phospholipid-binding activities. BSP-30K binds to spermatozoa upon ejaculation and is thought to play a role in sperm capacitation. We have determined its amino acid sequence, disulfide bonds, and 0-glycosylation sites...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Calvete JJ,Mann K,Sanz L,Raida M,Töpfer-Petersen E

    更新日期:1996-12-09 00:00:00

  • The F-box protein FBXL18 promotes glioma progression by promoting K63-linked ubiquitination of Akt.

    abstract::F-box proteins play pivotal roles in multiple cellular processes; however, little is known about their functions in glioma progression. In this study, we found that expression of the F-box and leucine-rich repeat protein 18 (FBXL18) is significantly upregulated in glioma tissues. Depletion of FBXL18 in glioma cells su...

    journal_title:FEBS letters

    pub_type: 信件


    authors: Zhang J,Yang Z,Ou J,Xia X,Zhi F,Cui J

    更新日期:2017-01-01 00:00:00

  • The complete primary structure of GTP:AMP phosphotransferase from beef heart mitochondria.

    abstract::To complete the amino acid sequence of GTP:AMP phosphotransferase (MgGTP + AMP in equilibrium with MgGDP + ADP) from beef heart mitochondria it was necessary to sequence an intermediate region of about 33 residues after position 102 [(1984) Eur. J. Biochem. 143, 331-339] and find a suitable overlap with the rest of th...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tomasselli AG,Frank R,Schiltz E

    更新日期:1986-07-07 00:00:00

  • TLC immunostaining characterization of Clostridium botulinum type A neurotoxin binding to gangliosides and free fatty acids.

    abstract::The receptor structure of Clostridium botulinum neurotoxin type A was analysed by TLC immunostaining. GQ1b was found to be the most potent receptor, and the neurotoxin also bound to GT1b and GD1a, but not to GM3, GM2, GM1, GD3, GD1b and GT1a. Optimum binding of neurotoxin to the ganglioside appeared in 0.01 M phosphat...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Takamizawa K,Iwamori M,Kozaki S,Sakaguchi G,Tanaka R,Takayama H,Nagai Y

    更新日期:1986-06-09 00:00:00

  • Identification and characterization of the hemoglobin-binding domain of hemoglobin receptor in Leishmania.

    abstract::Leishmania internalize hemoglobin (Hb) via a specific receptor (HbR) for their survival. To identify the Hb-binding domain of HbR, we cloned and expressed several truncated proteins of HbR and determined their ability to bind Hb. Our findings reveal that 90% of Hb-binding activity is retained in HbR41-80 in comparison...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Rastogi R,Verma JK,Singh V,Krishnamurthy G,Sood C,Kapoor A,Kumar K,Ansari I,Mukhopadhyay A

    更新日期:2020-12-14 00:00:00

  • The seventh zinc finger motif of A20 is required for the suppression of TNF-α-induced apoptosis.

    abstract::The ubiquitin-editing enzyme A20 suppresses nuclear factor-κB (NF-κB) activation and tumor necrosis factor-α (TNF-α)-induced apoptosis in a deubiquitinating and ubiquitin ligase activity-dependent manner. Although recent studies revealed that A20 regulates NF-κB independently of its enzymatic activity through its seve...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Yamaguchi N,Yamaguchi N

    更新日期:2015-05-22 00:00:00

  • AP-site cleavage activity of tyrosyl-DNA phosphodiesterase 1.

    abstract::APE-independent base excision repair (BER) pathway plays an important role in the regulation of DNA repair mechanisms. In this study it has been found that recently discovered tyrosyl-DNA phosphodiesterase 1 (Tdp1) catalyzes the AP site cleavage reaction to generate breaks with the 3'- and 5'-phosphate termini. The re...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lebedeva NA,Rechkunova NI,Lavrik OI

    更新日期:2011-02-18 00:00:00