Restriction of intramolecular movements within the Cry1Aa toxin molecule of Bacillus thuringiensis through disulfide bond engineering.

abstract：:The dinuclear paramagnetic center of the soluble CuA domain of the cytochrome c oxidase from Bacillus subtilis has been studied using 1H NMR. The spectrum possesses remarkably sharp shifted resonances. Comparison with the spectrum of the CuA amicyanin variant provides the spin density distribution in the CuA site of c...

journal_title：FEBS letters

authors： Dennison C,Berg A,de Vries S,Canters GW

更新日期：1996-10-07 00:00:00

abstract：:The physical properties of amino acids were investigated in order to evaluate their possible relationship to the assignment of codons for amino acids in the genetic code. A comparison of the interconversion probability between amino acids and the distances between the amino acids for individual physical properties rev...

journal_title：FEBS letters

authors： Sitaramam V

更新日期：1989-04-10 00:00:00

abstract：:CCN2 plays a critical role in the development of mesenchymal tissues such as cartilage and bone, and the binding of CCN2 to various cytokines and receptors regulates their signaling.By screening a protein array, we found that CCN2 could bind to fibroblast growth factor receptors (FGFRs) 2 and 3, with a higher affinity...

journal_title：FEBS letters

authors： Aoyama E,Kubota S,Takigawa M

更新日期：2012-12-14 00:00:00

abstract：:During lactation glucose metabolism in paraovarian adipocytes is characterized by a 40 and 80% decrease of glucose incorporation into CO2 and fatty acids in the presence of insulin. In contrast with the stimulation by insulin of glucose incorporation into lactate, glycerol remains unchanged. As a result, insulin sensi...

journal_title：FEBS letters

authors： Burnol AF,Guerre-Millo M,Lavau M,Girard J

更新日期：1986-01-06 00:00:00

abstract：:Phenylalanine ammonia-lyase immobilized within semipermeable microcapsules has an assayed enzyme activity which is 20% +/- 4% of the enzyme in free solution. The Km for the immobilized enzyme remained the same as that of the free enzyme. The pH optimum also remained unchanged at pH 8.5 +/- 1.0. At the lower pH range, ...

journal_title：FEBS letters

authors： Bourget L,Chang TM

更新日期：1985-01-21 00:00:00

abstract：:On the basis of its predicted homology to human telomerase reverse transcriptase (hTERT), a cDNA for Arabidopsis thaliana TERT (AtTERT) has now been isolated from cultured cells. The cDNA contains an open reading frame of 3372 bp, encoding a protein with a predicted size of 131 kDa and isoelectric point of 9.9. The At...

journal_title：FEBS letters

authors： Oguchi K,Liu H,Tamura K,Takahashi H

更新日期：1999-09-03 00:00:00

abstract：:Interleukin-8 (neutrophil-activating factor; NAP-1) has been crystallized by the vapour diffusion technique to give single crystals suitable for three-dimensional structural study at a resolution higher than 2.4 A. The crystals belong to the space group P3(1)21 or P3(2)21 and have unit cell dimensions a = b = 40.9 A, ...

journal_title：FEBS letters

authors： Auer M,Kallen J,Schleischitz S,Walkinshaw MD,Wasserbauer E,Ehn G,Lindley IJ

更新日期：1990-06-04 00:00:00

abstract：:Tropine (tropan-3 alpha-ol) is an intermediate in the formation of hyoscyamine. An acyltransferase activity that can acetylate tropine using acetylcoenzyme A as cosubstrate has been found in transformed root cultures of Datura stramonium. A further acyltransferase activity that acetylates pseudotropine (tropan-3 beta-...

journal_title：FEBS letters

authors： Robins RJ,Bachmann P,Robinson T,Rhodes MJ,Yamada Y

更新日期：1991-11-04 00:00:00

abstract：:Polyinosinic-polycytidylic acid, a potent inducer of inducer of interferon (IFN) production and activator of some IFN-induced enzymes, inhibits [3H]uridine incorporation into the RNA of vesicular stomatitis virus even in the absence of IFN synthesis, transiently triggers the breakdown of inositol phospholipids and act...

journal_title：FEBS letters

authors： Premecz G,Markovits A,Bagi G,Farkas T,Földes I

更新日期：1987-12-21 00:00:00

abstract：:It was determined that flavan-3-ols and procyanidins have an inhibitory effect on angiotensin I converting enzyme (ACE) activity, and the effect was dependent on the number of epicatechin units forming the procyanidin. The inhibition by flavan-3-ols and procyanidins was competitive with the two substrates assayed: N-h...

journal_title：FEBS letters

authors： Actis-Goretta L,Ottaviani JI,Keen CL,Fraga CG

更新日期：2003-12-18 00:00:00

abstract：:The effect of Rhodococcus fascians, the causal agent of leafy gall disease, on the mitotic behavior of synchronized tobacco Bright Yellow-2 (BY-2) cells was investigated. Incubation of aphidicolin-synchronized BY-2 cells with R. fascians cells specifically resulted in a broader mitotic index peak, an effect that was l...

journal_title：FEBS letters

authors： Temmerman W,Ritsema T,Simón-Mateo C,Van Montagu M,Mironov V,Inzé D,Goethals K,Holsters M

更新日期：2001-03-09 00:00:00

abstract：:NELL1 is an extracellular protein inducing osteogenic differentiation and bone formation of osteoblastic cells. To elucidate the intracellular signaling cascade evoked by NELL1, we have shown that NELL1 protein transiently activates the MAPK signaling cascade, induces the phosphorylation of Runx2, and promotes the rap...

journal_title：FEBS letters

authors： Bokui N,Otani T,Igarashi K,Kaku J,Oda M,Nagaoka T,Seno M,Tatematsu K,Okajima T,Matsuzaki T,Ting K,Tanizawa K,Kuroda S

更新日期：2008-01-23 00:00:00

abstract：:Compared with algal and cyanobacterial cytochrome c(6), cytochrome c(6A) from higher plants contains an additional loop of 12 amino acid residues. We have determined the first crystal structure of cytochrome c(6A) from Arabidopsis thaliana at 1.5 Angstrom resolution in order to help elucidate its function. The overall...

journal_title：FEBS letters

authors： Chida H,Yokoyama T,Kawai F,Nakazawa A,Akazaki H,Takayama Y,Hirano T,Suruga K,Satoh T,Yamada S,Kawachi R,Unzai S,Nishio T,Park SY,Oku T

更新日期：2006-06-26 00:00:00

abstract：:The gene trap methodology is a powerful tool to characterize novel genes and analyze their importance in biological phenomena. It is based on the use of mouse embryonic stem cells and reporter vectors designed to randomly integrate into the genome, tagging an insertion site and generating a mutation. Theoretically, al...

journal_title：FEBS letters

authors： Cecconi F,Meyer BI

更新日期：2000-08-25 00:00:00

abstract：:The known members of inhibitor of growth (ING) gene family are considered as candidate tumor suppressor genes. ING4, a novel member of ING family, is recently reported to interact with tumor suppressor p53, p300 (a major component of histone acetyl transferase complexes), and p65(RelA) subunit of NF-kappaB. In this st...

journal_title：FEBS letters

authors： Zhang X,Xu LS,Wang ZQ,Wang KS,Li N,Cheng ZH,Huang SZ,Wei DZ,Han ZG

更新日期：2004-07-16 00:00:00

abstract：:Several protein kinase C (PKC) isozymes were analyzed by immunoblot and immunocytochemistry in cultures of hippocampal neurones at several stages of differentiation. Our findings reveal the existence of two distinct patterns of expression. Firstly, conventional PKC isozymes alpha, beta and gamma, that are expressed at...

journal_title：FEBS letters

authors： Tejero-Díez P,Rodríguez-Sánchez P,Díez-Guerra FJ

更新日期：1995-04-24 00:00:00

abstract：:A mouse somatostatin (SS) receptor cDNA was cloned from neuroblastoma x glioma (NG108-15) cells. The sequence is almost identical to that of the mouse SSTR2 receptor [(1992) Proc. Natl. Acad. Sci. USA 89, 251)] but lacks about 300 nucleotides between transmembrane domain VII and the C-terminus. This spliced variant of...

journal_title：FEBS letters

authors： Vanetti M,Kouba M,Wang X,Vogt G,Höllt V

更新日期：1992-10-26 00:00:00

abstract：:Myotonic dystrophy protein kinase (DMPK) and Rho-kinase are related. An important function of Rho-kinase is to phosphorylate the myosin-binding subunit of myosin phosphatase (MYPT1) and inhibit phosphatase activity. Experiments were carried out to determine if DMPK could function similarly. MYPT1 was phosphorylated by...

journal_title：FEBS letters

authors： Murányi A,Zhang R,Liu F,Hirano K,Ito M,Epstein HF,Hartshorne DJ

更新日期：2001-03-30 00:00:00

abstract：:Anandamide (AEA) exhibits anti-inflammatory effects. However, its role in the periodontal field remains unknown. Here, we found that gingival crevicular fluid contained a detectable level of AEA. The cannabinoid receptors CB1 and CB2 were expressed by human gingival fibroblasts (HGFs), and markedly upregulated under p...

journal_title：FEBS letters

authors： Nakajima Y,Furuichi Y,Biswas KK,Hashiguchi T,Kawahara K,Yamaji K,Uchimura T,Izumi Y,Maruyama I

更新日期：2006-01-23 00:00:00

abstract：:The phytocannabinoid Delta(9)-Tetrahydrocannabinol (Delta(9)-THC), the main psychoactive cannabinoid in cannabis, activates a number of signalling cascades including p53. This study examines the role of Delta(9)-THC in regulating the p53 post-translational modifier proteins, Murine double minute (Mdm2) and Small Ubqui...

journal_title：FEBS letters

authors： Gowran A,Murphy CE,Campbell VA

更新日期：2009-11-03 00:00:00

abstract：:Insulin causes a translocation of glucose transporters from intracellular microsomes to the plasma membrane in adipocytes. To determine whether insulin has a similar effect in rat hindlimb muscles, we used glucose-inhibitable cytochalasin B binding to estimate the number of glucose transporters in membrane fractions f...

journal_title：FEBS letters

authors： Klip A,Ramlal T,Young DA,Holloszy JO

更新日期：1987-11-16 00:00:00

abstract：:Phospholamban (PLB), an integral membrane protein of cardiac sarcoplasmic reticulum (SR), is described as the regulator of the Ca(2+)-ATPase pump, via its phosphorylation-dephosphorylation of Ser-16. Recently it has been shown that a direct interaction between the N-terminal hydrophilic domain of PLB and Ca(2+)-ATPase...

journal_title：FEBS letters

authors： Terzi E,Poteur L,Trifilieff E

更新日期：1992-09-14 00:00:00

abstract：:Zinc (Zn) deficiency is often associated with low plasma vitamin A (retinol) concentrations. It has been suggested that the reduction in plasma retinol is secondary to reduced liver retinol binding protein (RBP) synthesis. In the present study, RBP expression was determined in HepG2 cells cultured in either Zn adequat...

journal_title：FEBS letters

authors： Satre MA,Jessen KA,Clegg MS,Keen CL

更新日期：2001-03-02 00:00:00

abstract：:vp165 (or gp160) is an aminopeptidase that has been identified as one of the major proteins of the GLUT4-containing vesicles. In the present study we have determined the degree of co-localization between vp165 and GLUT4 in rat adipose cells and used perturbation by wortmannin to assess the exocytic and endocytic steps...

journal_title：FEBS letters

authors： Malide D,St-Denis JF,Keller SR,Cushman SW

更新日期：1997-06-16 00:00:00

abstract：:Cholesterol is one of the major lipids of plasma membranes. Recently, we have shown that cholesterol depletion by methyl-beta-cyclodextrin (M beta CD) induces the activation of the Wnt/beta-catenin pathway and enhances myogenic differentiation. Here, we show that M beta CD-conditioned media accelerates myogenesis in a...

journal_title：FEBS letters

authors： Portilho DM,Martins ER,Costa ML,Mermelstein CS

更新日期：2007-12-22 00:00:00

abstract：:The action of substrate analogs containing the tetrazolyl group instead of the C-terminal carboxy group on the peptidase activity of carboxypeptidase A is studied. The analogs compete with the substrate for the secondary binding site thus showing activation phenomena. ...

journal_title：FEBS letters

authors： Kojro E,Willhardt I,Römbach A,Grzonka Z,Hermann P

更新日期：1987-02-09 00:00:00

abstract：:It has been suggested that alpha-dystroglycan links the dystrophin-associated protein complex and extracellular matrix and that the absence of dystrophin and alpha-dystroglycan in Duchenne muscular dystrophy (DMD) may lead to the breakdown of this linkage. In the present study, myotubes from DMD patients and murine X-...

journal_title：FEBS letters

authors： Angoli D,Corona P,Baresi R,Mora M,Wanke E

更新日期：1997-05-26 00:00:00

abstract：:It has recently been discovered that glutathione-dependent formaldehyde dehydrogenase (FALDH) exhibits a strong S-nitrosoglutathione reductase activity. Plants use NO and S-nitrosothiols as signaling molecules to activate defense mechanisms. Therefore, it is interesting to investigate the regulation of FALDH by mechan...

journal_title：FEBS letters

authors： Díaz M,Achkor H,Titarenko E,Martínez MC

更新日期：2003-05-22 00:00:00

abstract：:Bispecific miniantibodies were constructed by genetically fusing the C(H)1 domain of an IgG1 to the C-terminus of a single-chain Fv fragment (scFv-425), specific for the EGF receptor, and fusing the C(L) domain of a kappa light chain to the C-terminus of a scFv specific for CD2 (scFv-M1). An efficient dicistronic gene...

journal_title：FEBS letters

authors： Müller KM,Arndt KM,Strittmatter W,Plückthun A

更新日期：1998-01-30 00:00:00

abstract：:Tissue plasminogen activator was purified in high yield from pig heart by immunoaffinity chromatography and characterized by analysis of the glycosylation pattern and the N-terminal amino acid sequence. Comparisons with the human enzyme reveals residue exchanges in the A-chain at positions 3 (porcine Arg/human Gln) an...

journal_title：FEBS letters

authors： Pohl G,Jörnvall H,Kok P,Wallén P

更新日期：1986-09-01 00:00:00