Abstract:
:Phenylalanine ammonia-lyase immobilized within semipermeable microcapsules has an assayed enzyme activity which is 20% +/- 4% of the enzyme in free solution. The Km for the immobilized enzyme remained the same as that of the free enzyme. The pH optimum also remained unchanged at pH 8.5 +/- 1.0. At the lower pH range, enzyme activity is higher for the immobilized enzyme. Daily oral administration of microencapsulated phenylalanine ammonia-lyase to phenylketonuric rats decreased the systemic phenylalanine level by 35 +/- 8% in 2 days (P less than 0.05) and by 75 +/- 8% in 7 days (P less than 0.001).
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Bourget L,Chang TMdoi
10.1016/0014-5793(85)80219-2subject
Has Abstractpub_date
1985-01-21 00:00:00pages
5-8issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(85)80219-2journal_volume
180pub_type
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