Abstract:
:Cholesterol is one of the major lipids of plasma membranes. Recently, we have shown that cholesterol depletion by methyl-beta-cyclodextrin (M beta CD) induces the activation of the Wnt/beta-catenin pathway and enhances myogenic differentiation. Here, we show that M beta CD-conditioned media accelerates myogenesis in a similar way as M beta CD does, suggesting that the effects induced by M beta CD could be caused by soluble factors present in the culture medium. Soluble Wnt-3 protein is significantly enhanced in M beta CD-conditioned medium. Wnt-3a-enriched media induces myogenesis as much as M beta CD does, whereas Wnt-5a-enriched media inhibits. We suggest that Wnt-3a is involved in the myogenic induction observed after cholesterol depletion.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Portilho DM,Martins ER,Costa ML,Mermelstein CSdoi
10.1016/j.febslet.2007.11.047subject
Has Abstractpub_date
2007-12-22 00:00:00pages
5787-95issue
30eissn
0014-5793issn
1873-3468pii
S0014-5793(07)01191-Xjournal_volume
581pub_type
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