Abstract:
:Disruption of yeast spheroplasts by DEAE-dextran in isoosmotic conditions allows isolation of relatively undamaged subcellular fractions from yeast. The preservation of mitochondria and vacuoles permits the simultaneous isolation of the cytosol with negligible contamination by vacuolar proteinases and therefore, virtually eliminates proteolytic artefacts.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Schwencke J,Canut H,Flores Adoi
10.1016/0014-5793(83)80512-2subject
Has Abstractpub_date
1983-06-13 00:00:00pages
274-80issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(83)80512-2journal_volume
156pub_type
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