Abstract:
:The reduction of methyl-coenzyme M (CH3SCoM) to methane in methanogenic bacteria is dependent on component B (N-7-mercaptoheptanoyl-O-phospho-L-threonine, HSHTP). We report here that S-methyl-component B (N-7-(methylthio)heptanoyl-O-phospho-L-threonine, CH3SHTP) can substitute for neither CH3SCoM nor HSHTP in the methyl-CoM reductase reaction. Rather, CH3SHTP proved to be an inhibitor competitive with HSHTP (apparent Ki = 6 microM) and noncompetitive with CH3SCoM. These results make it very unlikely that HSHTP functions as a methyl group carrier. A role for HSHTP as direct electron donor for CH3SCoM reduction to CH4 is proposed.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Ellermann J,Kobelt A,Pfaltz A,Thauer RKdoi
10.1016/0014-5793(87)80846-3subject
Has Abstractpub_date
1987-08-17 00:00:00pages
358-62issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(87)80846-3journal_volume
220pub_type
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