Abstract:
:Di(1,N6-ethenoadenosine)5',5'''-P1,P4-tetraphosphate, epsilon-(Ap4A), a fluorescent analog of Ap4A has been synthesized by reaction of 2-chloroacetaldehyde with Ap4A. At neutral pH this Ap4A analog presents characteristics maxima at 265 and 274 nm, shoulders at ca 260 and 310 nm and moderate fluorescence (lambda exc 307 nm, lambda em 410 nm). Enzymatic hydrolysis of the phosphate backbone produced a slight hyperchromic effect but a notorious increase of the fluorescence emission. Cytosolic extracts from adrenochromaffin tissue as well as cultured chromaffin cells were able to split epsilon(Ap4A) and catabolize the resulting epsilon-nucleotide moieties up to epsilon-Ado.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Rotilán P,Ramos A,Pintor J,Torres M,Miras-Portugal MTdoi
10.1016/0014-5793(91)80334-ysubject
Has Abstractpub_date
1991-03-25 00:00:00pages
371-4issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(91)80334-Yjournal_volume
280pub_type
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