Abstract:
:Bacterial DNA supercoiling is controlled by balancing the supercoiling activity of DNA gyrase and the relaxing activity of DNA topoisomerase I. We have characterized the gyrB gene from a top A deletion mutant of Escherichia coli (DM800) that has a compensatory mutation in gyrB, lowering the activity of gyrase 10-fold, and thereby redressing the intracellular level of supercoiling. The mutant gene differs from the wild type in carrying three rather than two direct tandem repeats of a 6 bp sequence encoding Ala-Arg. We suggest this novel mutation affects domain spacing and was generated by an unequal crossing over event, possibly involving gyrase.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
McEachern F,Fisher LMdoi
10.1016/0014-5793(89)80931-7subject
Has Abstractpub_date
1989-08-14 00:00:00pages
67-70issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(89)80931-7journal_volume
253pub_type
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