Regulation of DNA supercoiling in Escherichia coli: genetic basis of a compensatory mutation in DNA gyrase.

Abstract:

:Bacterial DNA supercoiling is controlled by balancing the supercoiling activity of DNA gyrase and the relaxing activity of DNA topoisomerase I. We have characterized the gyrB gene from a top A deletion mutant of Escherichia coli (DM800) that has a compensatory mutation in gyrB, lowering the activity of gyrase 10-fold, and thereby redressing the intracellular level of supercoiling. The mutant gene differs from the wild type in carrying three rather than two direct tandem repeats of a 6 bp sequence encoding Ala-Arg. We suggest this novel mutation affects domain spacing and was generated by an unequal crossing over event, possibly involving gyrase.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

McEachern F,Fisher LM

doi

10.1016/0014-5793(89)80931-7

subject

Has Abstract

pub_date

1989-08-14 00:00:00

pages

67-70

issue

1-2

eissn

0014-5793

issn

1873-3468

pii

0014-5793(89)80931-7

journal_volume

253

pub_type

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