Abstract:
:Engrailed-2 (En-2) belongs to an evolutionarily conserved family of DNA binding homeodomain-containing proteins that are expressed in mammalian brain during development. Here, we demonstrate that serine 267 in the homeodomain of En-2 is phosphorylated by protein kinase A (PKA) in forskolin-treated COS-7 cells. Furthermore, we analyze the physiological function of En-2 phosphorylation by PKA. The nuclear localization of En-2 is not influenced by the phosphorylation of serine 267. However, substitution of serine 267 with alanine resulted in increased binding of En-2 to DNA, while replacing serine 267 with glutamic acid resulted in decreased En-2 DNA binding. These results suggest that the transcriptional activity of En-2 is regulated by PKA.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Hjerrild M,Stensballe A,Jensen ON,Gammeltoft S,Rasmussen TEdoi
10.1016/j.febslet.2004.05.009keywords:
subject
Has Abstractpub_date
2004-06-18 00:00:00pages
55-9issue
1-3eissn
0014-5793issn
1873-3468pii
S0014579304006076journal_volume
568pub_type
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