Insulin processing and action in adipocytes: evidence for generation of insulin-containing vesicles by leupeptin and monensin.

Abstract:

:Incubation of adipocytes with 125I-insulin plus leupeptin or monensin, but not chloroquine, resulted in the appearance of a novel peak of 125I-insulin (modal density about 1.20 g/ml) on density gradient centrifugation; the appearance of the peak depended on the presence of specific insulin receptors on the cell surface. The fractions comprising this peak contained vesicles, probably originating from the Golgi apparatus, and dit not appear to be contaminated with lysosomes, mitochondria or plasma membrane. Entrapment of insulin in these vesicles per se did not prevent the activation of glucose transport, acetyl-CoA carboxylase or pyruvate dehydrogenase by insulin.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Jeffrey IW,Flint DJ,Vernon RG,Mayer RJ

doi

10.1016/0014-5793(85)80352-5

subject

Has Abstract

pub_date

1985-03-25 00:00:00

pages

451-4

issue

2

eissn

0014-5793

issn

1873-3468

pii

0014-5793(85)80352-5

journal_volume

182

pub_type

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