Abstract:
:Incubation of adipocytes with 125I-insulin plus leupeptin or monensin, but not chloroquine, resulted in the appearance of a novel peak of 125I-insulin (modal density about 1.20 g/ml) on density gradient centrifugation; the appearance of the peak depended on the presence of specific insulin receptors on the cell surface. The fractions comprising this peak contained vesicles, probably originating from the Golgi apparatus, and dit not appear to be contaminated with lysosomes, mitochondria or plasma membrane. Entrapment of insulin in these vesicles per se did not prevent the activation of glucose transport, acetyl-CoA carboxylase or pyruvate dehydrogenase by insulin.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Jeffrey IW,Flint DJ,Vernon RG,Mayer RJdoi
10.1016/0014-5793(85)80352-5subject
Has Abstractpub_date
1985-03-25 00:00:00pages
451-4issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(85)80352-5journal_volume
182pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::The effects of guanine nucleotides on arachidonic acid (AA) release were studied in intact and saponin-permeabilized human platelets. While GTP[S] itself caused a stimulation of AA release in permeabilized cells, GTP[S], GDP[S], GTP, ATP and other nucleotides inhibited AA release in response to thrombin and other agon...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80107-8
更新日期:1988-07-18 00:00:00
abstract::We have identified an Arabidopsis thaliana cDNA, designated as AtTBP1, encoding a protein with a predicted size of 70.6 kDa that specifically binds to the plant telomeric repeat sequence TTTAGGG. AtTBP1 is present as a single-copy gene in Arabidopsis genome and is expressed ubiquitously in various organs. AtTBP1 has a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02685-0
更新日期:2001-08-10 00:00:00
abstract::We analyzed the expression of aquaporins (AQPs) in the small intestine to elucidate their functions, and found that AQP9, which had not previously been detected there, is present in duodenum, jejunum, and ileum. AQP9 is expressed in colon as well, but not in stomach. Also, its expression in these intestinal sections i...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00256-4
更新日期:2003-04-10 00:00:00
abstract::The phosphatidylinositol 3-kinase/Akt pathway is responsible for key aspects of tumor progression, and is frequently hyperactivated in cancer. We have recently identified palladin, an actin-bundling protein that functions to control the actin cytoskeleton, as an Akt1-specific substrate that inhibits breast cancer cell...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.10.056
更新日期:2010-12-01 00:00:00
abstract::cDNA clones encoding the 45 kDa protein were isolated from a rat olfactory epithelium cDNA library and their inserts were sequenced. The reconstructed protein sequence comprises 400 amino acids with a calculated molecular mass of 46,026 Da. A homology was revealed between the amino acid sequence of the 45 kDa protein ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00470-6
更新日期:1999-04-30 00:00:00
abstract::Nerve growth factor (NGF), basic fibroblast growth factor (bFGF), dibutyryl cAMP and forskolin, known differentiating agents for pheochromocytoma PC12 cells, induced sustained activation of sphingosine kinase, the enzyme responsible for the formation of the sphingolipid second messenger, sphingosine-1-phosphate, which...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01277-5
更新日期:1997-11-10 00:00:00
abstract::Antithrombin Glasgow is a hereditary abnormal antithrombin that has lost thrombin inhibitory activity. It was isolated from the plasma of a 41-year-old male with a history of thrombotic events. Antithrombin Glasgow was purified from plasma using heparin-Sepharose chromatography at pH 7.4 eluting with increasing concen...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80841-x
更新日期:1988-04-25 00:00:00
abstract::A large number of protein-protein interactions involved turn or loop regions. The excised linear peptides from these regions reveal complex conformational averaging. To circumvent this motional averaging and to stabilize the beta-turn conformation, extensive effort has been devoted to the design of constrained peptido...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00007-v
更新日期:1995-02-13 00:00:00
abstract::Peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1alpha), a highly inducible transcriptional coactivator regulating energy homeostasis, is down-regulated in hepatoma tissues. To dissect its role in hepato-tumorigenesis, Ingenuity Pathway Analysis was applied to construct pathways affected by P...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.01.033
更新日期:2008-03-05 00:00:00
abstract::All eukaryotic cells enclose their genome within a dedicated, membrane-bound organelle termed the nucleus, which functions to partition gene transcription from sites of protein translation in the cytoplasm. Despite a great deal of research effort, basic questions about chromosome structure and gene expression mechanis...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2008.04.029
更新日期:2008-06-18 00:00:00
abstract::Tripeptidyl peptidase I (TPP-I) is a lysosomal enzyme that cleaves tripeptides from the N-terminus of polypeptides. A comparison of TPP-I amino acid sequences with sequences derived from an EST database suggested that TPP-I is identical to a pepstatin-insensitive carboxyl proteinase of unknown specificity which is mut...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01683-4
更新日期:1999-01-25 00:00:00
abstract::Superoxide reacts with nitric oxide to form peroxynitrite, a potent oxidising agent which may contribute to tissue damage in pathological situations such as inflammation and ischaemia/reperfusion. One mechanism by which oxidative stress damages tissues is the induction of a specific Cyclosporin A-sensitive mitochondri...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00461-7
更新日期:1994-05-30 00:00:00
abstract::Treatment of yeast phosphoglycerate kinase (PGK) with trypsin results in a fourfold increase in the Vmax of this enzyme, without affecting the Km. This activation is shown to be due to the removal of the C-terminal lysine residue. The C-terminal sequence folds back over the N-terminal domain and contacts the extreme N...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00348-1
更新日期:1996-04-29 00:00:00
abstract::We quantitatively measured protective antigen (PA) binding to human cells targeted by anthrax lethal toxin (LT). Affinities were less than 50 nM for all cells, but differentiated cells (macrophages and neutrophils) had significantly increased PA binding and endothelial cells demonstrated the most binding. Combined wit...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.06.070
更新日期:2006-07-24 00:00:00
abstract::After initial purification of ovine placental lactogen (oPL) using the procedures described previously [(1976) Endocrinology 98, 65-75], the oPL preparation was further purified by high-performance liquid chromatography (HPLC) using an anionic exchange column (Bio-Sil TSK DEAE-2-SW). Two forms of oPL with different re...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80491-4
更新日期:1986-04-21 00:00:00
abstract::Using antibodies recognizing the phosphorylation state of specific sites, phosphorylation states of tau were monitored in fetal rat primary cultured neurons. When cultured neurons were treated with okadaic acid (OA) or calyculin A (CalA) at concentrations sufficient to inhibit protein phosphatase 2A (PP2A), phosphoryl...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01292-0
更新日期:1995-12-04 00:00:00
abstract::Human legumain was characterized following overexpression in a murine cell line as the C-terminal Ig-fusion protein. Upon acid treatment, the prolegumain autoproteolyzed distal to two aspartic acid residues to yield a highly active form. The ability of mature legumain to cleave after aspartic acid residues was confirm...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01281-2
更新日期:1998-10-30 00:00:00
abstract::The c-Jun N-terminal kinase (JNK) signalling pathway has an established role in cellular stress signalling, cell survival and tumorigenesis. Here, we demonstrate that inhibition of JNK signalling results in partial delocalization of the RNA helicase DDX21 from the nucleolus to the nucleoplasm, increased nucleolar mobi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.08.004
更新日期:2008-09-03 00:00:00
abstract::Inducible nitric oxide (NO) synthase (iNOS) appears to be a marker of tumor progression in colon carcinogenesis. Here we investigated effects of NO on selected chemokines that differentially regulate angiogenesis, namely pro-angiogenic interleukin (IL)-8 as well as tumor-suppressive interferon-inducible protein-10 (IP...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/S0014-5793(04)00275-3
更新日期:2004-04-09 00:00:00
abstract::We examined whether a proposed spatial proximity between Asp114(2.50) and Asn332(7.49) affected the functional properties of the mu opioid receptor. The D114(2.50)N mutant had reduced binding affinities for morphine, DAMGO and CTAP, but not for naloxone and [3H]diprenorphine; this mutation also abolished agonist-induc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00316-6
更新日期:1999-03-26 00:00:00
abstract::Regulation of supra-macromolecular composition and catalytic activity of a heterodimeric enzyme, gamma-glutamyltransferase, in the system of Aerosol OT (sodium bis(2-ethylhexyl) sulfosuccinate) reversed micelles in octane were studied. Variation of the surfactant hydration degree (parameter, determining dimensions of ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)81388-o
更新日期:1991-12-16 00:00:00
abstract::We examined the effects of histidine residues that were artificially introduced into complementarity-determining regions of antibodies on antigen-antibody interactions at different pH values. Using a monoclonal antibody specific for hen egg-white lysozyme and three mutant antibodies that contained a histidine residue,...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80745-3
更新日期:1992-08-31 00:00:00
abstract::We have determined the first de novo position of the secondary quinone QB in the Rhodobacter sphaeroides reaction center (RC) using phases derived by the single wavelength anomalous dispersion method from crystals with selenomethionine substitution. We found that in frozen RC crystals, QB occupies primarily the proxim...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.06.042
更新日期:2004-07-16 00:00:00
abstract::cDNAs encoding aquaporins PIP1;1, PIP2;1, and TIP1;1 were isolated from Mimosa pudica (Mp) cDNA library. MpPIP1;1 exhibited no water channel activity; however, it facilitated the water channel activity of MpPIP2;1 in a phosphorylation-dependent manner. Mutagenesis analysis revealed that Ser-131 of MpPIP1;1 was phospho...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.06.082
更新日期:2005-08-15 00:00:00
abstract::Protein gene product 9.5 (PGP 9.5), which in the normal nervous system is restricted to certain neurons, has been detected in two glioma cell lines, rat C6 and human GL15, by immunoblotting and immunocytochemistry. Its expression in these cells depends on the cellular growth state, being maximal between the first and ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)81242-z
更新日期:1991-09-23 00:00:00
abstract::The interaction of Fas (Apo-1) and TNF receptor-1 with their respective ligands can lead to cell death. The so-called death domain, a sequence motif present in the cytoplasmic portion of the two receptors, has been identified as a critical structural element involved in signal transduction that leads to apoptosis. Her...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00931-x
更新日期:1995-09-11 00:00:00
abstract::In order to investigate oxysterol-mediated effects on the biliary system, we studied the effects of cholestan-3beta,5alpha,6beta-triol (TriolC) and 7-ketocholesterol (7KC) on gallbladder epithelial cells. We compared their cell proliferation effects in cultured dog gallbladder epithelial cells (DGBE) to their effects ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01831-7
更新日期:2000-07-28 00:00:00
abstract::The relationship between different amino acids at the beta 6 position of hemoglobin and tetramer stability was addressed by a site-directed mutagenesis approach. Precipitation rates during mechanical agitation of oxyhemoglobins with Gln, Ala, Val, Leu and Trp at the beta 6 position increased 2, 5, 13, 21 and 53 times,...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81130-r
更新日期:1993-01-02 00:00:00
abstract::The phytocannabinoid Delta(9)-Tetrahydrocannabinol (Delta(9)-THC), the main psychoactive cannabinoid in cannabis, activates a number of signalling cascades including p53. This study examines the role of Delta(9)-THC in regulating the p53 post-translational modifier proteins, Murine double minute (Mdm2) and Small Ubqui...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.09.056
更新日期:2009-11-03 00:00:00
abstract::Using the yeast two hybrid system and overlay assays we identified a putative rholrac effector, citron, which interacts with the GTP-bound forms of rho and rac1, but not with cdc42. Extensive homologies to known proteins were not observed. This 183 kDa protein contains a C6H2 zinc finger, a PH domain, and a long coile...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01351-2
更新日期:1995-12-18 00:00:00