Abstract:
:The mitochondrial myopathy, encephalopathy, lactic acidosis and stroke-like episodes syndrome (MELAS) is a rare congenital disorder of mitochondrial DNA. Five single nucleotide substitutions within the human mitochondrial tRNALeu(UUR) gene have been reported to be associated with MELAS. Here, we provide in vitro evidence that the aminoacylation capacities of these five hmtRNALeu(UUR) transcripts are reduced to different extents relative to the wild-type hmtRNALeu(UUR) transcript. A thermal denaturation experiment showed that the A3243G and T3291C mutants, which were the least charged by LeuRS, have fragile structures. In addition, the T3291C mutant can inhibit aminoacylation of the wild-type hmtRNALeu(UUR), indicating that it may act as an inhibitor in the mitochondrial heteroplasmic environment.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Hao R,Yao YN,Zheng YG,Xu MG,Wang EDdoi
10.1016/j.febslet.2004.11.004keywords:
subject
Has Abstractpub_date
2004-12-03 00:00:00pages
135-9issue
1-2eissn
0014-5793issn
1873-3468pii
S0014-5793(04)01354-7journal_volume
578pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Benzimidazoles are widely used as anthelmintic agents and systemic fungicides. In susceptible organisms, benzimidazoles bind to beta-tubulin and block microtubule polymerization. To further characterize this interaction, site-directed mutagenesis followed by gene replacement was used to change Saccharomyces cerevisiae...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00334-1
更新日期:1996-04-29 00:00:00
abstract::We investigated in vitro effects of recombinant human thrombopoietin (TPO), or c-Mpl ligand, on human platelets. TPO induced rapid dose-dependent tyrosine phosphorylation of several proteins. We identified Janus tyrosine kinases, Tyk2 and JAK2, and a member of STAT (signal transducers and activators of transcription) ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01072-m
更新日期:1995-10-23 00:00:00
abstract::The interaction of Fas (Apo-1) and TNF receptor-1 with their respective ligands can lead to cell death. The so-called death domain, a sequence motif present in the cytoplasmic portion of the two receptors, has been identified as a critical structural element involved in signal transduction that leads to apoptosis. Her...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00931-x
更新日期:1995-09-11 00:00:00
abstract::Interleukin-8 (neutrophil-activating factor; NAP-1) has been crystallized by the vapour diffusion technique to give single crystals suitable for three-dimensional structural study at a resolution higher than 2.4 A. The crystals belong to the space group P3(1)21 or P3(2)21 and have unit cell dimensions a = b = 40.9 A, ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80876-k
更新日期:1990-06-04 00:00:00
abstract::Incorporation of dioleoyl N-(monomethoxy polyethyleneglycol succinyl)phosphatidylethanolamine (PEG-PE) into large unilamellar liposomes composed of egg phosphatidylcholine:cholesterol (1:1) does not significantly increase the content leakage when the liposomes are exposed to 90% human serum at 37 degrees C, yet the li...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81016-h
更新日期:1990-07-30 00:00:00
abstract::The primary structure of nuclear factor-I (CTF/NF-I), a eukaryotic regulatory DNA-binding protein involved in both DNA replication and gene transcription, and the secondary structure predictable from it, are compared here with those of a number of prokaryotic acetylases. Hydropathy and Chou-Fasman analyses reveal that...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81039-q
更新日期:1990-10-29 00:00:00
abstract::The release of a GPI-linked oncofetal protein (PLAP) from HEp-2 cells has been studied as an indicator of endogenous PI-specific PLC activation. A non-hydrolysable GTP analogue, EGF and insulin all produced a dose-dependent release of PLAP from these cells. This indicates that an increase in PLC activity following eit...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80927-b
更新日期:1990-07-16 00:00:00
abstract::Recombinant porcine growth hormone (rPGH) was solubilised from inclusion bodies (IB's) using either 6 M guanidinium hydrochloride (GnHCl), 7.5 M urea or by a novel method using a cationic surfactant, cetyltrimethylammonium chloride (CTAC). Circular dichroism (CD) analysis of the secondary (2 degrees) structure of the ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80661-y
更新日期:1992-07-06 00:00:00
abstract::A new peptide, Tc1, containing only 23 amino acids closely packed by three disulfide bridges was isolated from the Amazonian scorpion Tityus cambridgei. It blocks reversibly the Shaker B K(+)-channels with a K(d) of 65 nM and displaces binding of noxiustoxin to mouse brain synaptosome membranes. It is the shortest kno...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)02253-5
更新日期:2000-12-08 00:00:00
abstract::The gene spa P (formerly designated as spa P1) encoding the Mr 185,000 surface antigen (I/II) of Streptococcus mutans, serotype c (NG5), has been sequenced. The gene (4683 bp) encodes a protein of 1561 amino acid residues including putative signal peptide (residues 1-38) and transmembrane (residues 1537-1556) sequence...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81632-1
更新日期:1989-11-20 00:00:00
abstract::Beta-thymosins sequester G-actin and preserve a pool of monomers of actin which constitute an important prerequisite for cellular function of the microfilament system. To study the influence of paraquat binding to G-actin on the interaction of G-actin with thymosin beta4 we determined the apparent dissociation constan...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00295-6
更新日期:1998-04-03 00:00:00
abstract::Granulocytes from human eosinophilic donors were incubated with arachidonic acid or 15-hydroxyeicosatetraenoic acid (15-HETE) and stimulated with the ionophore A23187. The eicosanoids were extracted with reversed-phase cartridges and subjected to RP-HPLC analysis. When extracts from eosinophil-enriched populations wer...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81078-6
更新日期:1989-09-11 00:00:00
abstract::The minimal functional enzyme unit of Na,K-ATPase consists of an alpha-beta complex. The alpha-subunit bears all functional domains of the enzyme and so far a regulatory role for the beta-subunit in the catalytic cycle has not been established. On the other hand, increasing experimental evidence suggests that the beta...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/0014-5793(91)80801-9
更新日期:1991-07-22 00:00:00
abstract::The crystal structure of the free form of IF1 from Mycobacterium tuberculosis has been determined at 1.47 A resolution. The structure adopts the expected OB fold and matches the high structural conservation among IF1 orthologues. In order to further explore the function of Mtb-IF1, we built a model of its interaction ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.01.051
更新日期:2010-03-05 00:00:00
abstract::Recently, it has been shown that really interesting new gene (RING)-in between ring finger (IBR)-RING domain-containing proteins, such as Parkin and Parc, are E3 ubiquitin ligases and are involved in regulation of apoptosis. In this report, we show that p53-inducible RING-finger protein (p53RFP), a p53-inducible E3 ub...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.09.105
更新日期:2006-02-06 00:00:00
abstract::A 569 bp probe against the beta-chain of hepatotropin was used to examine expression of RNA for this growth factor in human adult and foetal liver, foetal kidney and pancreas, and rat liver after partial hepatectomy. Low level expression of a 6 kb RNA occurred in human adult and normal rat liver. 70% hepatectomy incre...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81239-k
更新日期:1990-09-17 00:00:00
abstract::The Protein Data Bank (PDB) was established in 1971 as a repository for the three dimensional structures of biological macromolecules. Since then, more than 85000 biological macromolecule structures have been determined and made available in the PDB archive. Through analysis of the corpus of data, it is possible to id...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2012.12.029
更新日期:2013-04-17 00:00:00
abstract::Interfering RNA was used to suppress the expression of the genes At1g06680 and At2g30790 in Arabidopsis thaliana, which encode the PsbP-1 and PsbP-2 proteins, respectively, of Photosystem II. A phenotypic series of transgenic plants was recovered that expressed intermediate and low amounts of PsbP. Earlier we had docu...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.05.048
更新日期:2009-06-18 00:00:00
abstract::A wide variety of phospholipase A2 inhibitors blocks intra-Golgi protein transport reconstituted in a cell-free system. Phospholipase A2 activity detectable under the protein transport assay conditions is actually inhibited by the inhibitors. There is a good correlation between the inhibition of protein transport and ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81393-e
更新日期:1993-06-14 00:00:00
abstract::We have found that wheat germ agglutinin (WGA), a lectin that specifically binds to N-acetylglucosamine residues inhibits the in vitro transport of plasmid DNA, pJDB219, into yeast nuclei. Histochemical staining of the isolated nuclei with biotinylated WGA and streptavidin-biotinylated peroxidase complex revealed the ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80739-2
更新日期:1989-07-03 00:00:00
abstract::Human respiratory syncytial virus (HRSV) is the leading cause of lower respiratory tract disease in infants. The HRSV small hydrophobic (SH) protein plays an important role in HRSV pathogenesis, although its mode of action is unclear. Analysis of the ability of SH protein to induce membrane permeability and form homo-...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.05.006
更新日期:2010-07-02 00:00:00
abstract::The fate of newly synthesized human immunodeficiency virus type 1 env gp160 was examined in COS-1 cells. The results of morphological chase experiments involving cycloheximide demonstrated that gp160 was retained in the Golgi apparatus for longer than the half-life of the molecule. The degradation of gp160 was insensi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00614-x
更新日期:1996-07-15 00:00:00
abstract::The HBx protein of hepatitis B virus is involved in deregulation of cell cycle and development of hepatocellular carcinoma. Since c-Myc also plays an important role in cell proliferation and tumor development, we studied its regulation by HBx in a human hepatoma cell line. Co-expression of HBx and c-Myc resulted in in...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.12.034
更新日期:2006-01-23 00:00:00
abstract::Seminal plasmin (SPLN) a 47-residue peptide, isolated from bovine seminal plasma, exhibits antibacterial activity against Gram-positive and Gram-negative bacteria. Although SPLN strongly inhibits the transcription of various natural and synthetic templates by E. coli RNA polymerase in vitro, it also associates with mo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80535-o
更新日期:1992-06-01 00:00:00
abstract::When acquiring internal membranes and vesicular transport, eukaryotic cells started to synthesize sphingolipids and sterols. The physical differences between these and the glycerophospholipids must have enabled the cells to segregate lipids in the membrane plane. Localizing this event to the Golgi then allowed them to...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2009.10.020
更新日期:2010-05-03 00:00:00
abstract::Ubiquitin was extracted from testis of 4 mammals and purified to homogeneity by gel filtration chromatography. Amino acid compositions and NH2-terminal sequences were found to be identical in the 4 species and with calf thymus ubiquitin. Ubiquitin conformation was shown to be very sensitive to oxidation. Improved meth...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80318-x
更新日期:1984-04-24 00:00:00
abstract::Lysosomal/phagosomal membranes of mammalian cells are coated by highly conserved glycoproteins (lamps) that are thought to protect the membranes from degradation. Interestingly, we identified an amino acid sequence in human lamp-1 characteristic of a cleavage site for the Neisseria gonorrhoeae IgA1 protease. Furthermo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00163-4
更新日期:1997-03-17 00:00:00
abstract::Hterologous complementation of yeast mutants has enabled the isolation of genes encoding several families of amino acid transporters. Among them, NTR1 codes for a membrane protein with weak histidine transport activity. However, at the sequence level, NTR1 is related to rather non-specific oligopeptide transporters fr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00853-2
更新日期:1995-08-21 00:00:00
abstract::We have reported that histone deacetylase (HDAC) inhibitors activate a member of the INK4 family, the p19INK4d gene, causing G1 phase arrest. We report here that HDAC inhibitor, Trichostatin A, activates another member of the INK4 family, the p18INK4c gene, through its promoter in Jurkat cells. Interestingly, the acti...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.08.025
更新日期:2004-09-10 00:00:00
abstract::We have investigated the functional importance of nucleotide sequence differences between proximal (P-) and distal (D-) p53-binding elements in the MCK promoter. P- and D-elements normally co-operate to permit synergistic promoter activation by p53. Interestingly, we find that P-elements cannot co-operate with each ot...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00283-4
更新日期:1997-04-14 00:00:00