Abstract:
:We have investigated the functional importance of nucleotide sequence differences between proximal (P-) and distal (D-) p53-binding elements in the MCK promoter. P- and D-elements normally co-operate to permit synergistic promoter activation by p53. Interestingly, we find that P-elements cannot co-operate with each other. In contrast, co-operation between D-binding sites results in levels of p53-induced transcription far higher than those obtained by co-operation between P- and D-elements. These studies imply that distinct D- and P-p53-binding sites in the MCK promoter may dictate the promoter response to p53.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Jackson P,Yardley Gdoi
10.1016/s0014-5793(97)00283-4subject
Has Abstractpub_date
1997-04-14 00:00:00pages
271-4issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(97)00283-4journal_volume
406pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::We investigated in vitro effects of recombinant human thrombopoietin (TPO), or c-Mpl ligand, on human platelets. TPO induced rapid dose-dependent tyrosine phosphorylation of several proteins. We identified Janus tyrosine kinases, Tyk2 and JAK2, and a member of STAT (signal transducers and activators of transcription) ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01072-m
更新日期:1995-10-23 00:00:00
abstract::Using an all-atom, molecular dynamics-based, flexible docking method, the tertiary and quaternary structures of protofilaments of the "K3" fragment from beta(2)-microglobulin (residues Ser20-Lys41) were predicted at low pH in a continuous mixture of water and 2,2,2-trifluoroethanol (TFE). Tetramers with energies very ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.10.022
更新日期:2006-11-13 00:00:00
abstract::In human platelet membranes enhanced tyrosine phosphorylation of certain proteins was observed when Zn2+ instead of Mg2+ or Mn2+ was used as a divalent cation for the kinase reaction. An enhanced level of phosphate incorporation into tyrosine residues occurred into a 68 kDa polypeptide besides the 45 kDa and 105 kDa p...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)81232-8
更新日期:1988-08-01 00:00:00
abstract::We compared oxidative DNA damage in strictly anaerobic Prevotella melaninogenica, aerotolerant anaerobic Bacteroides fragilis, and facultative anaerobic Salmonella typhimurium after exposure to O2 or H2O2. Using HPLC with electrochemical detection, we measured 8-hydroxydeoxyguanosine (8OHdG) as a damage marker. O2 ind...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00497-4
更新日期:1999-05-07 00:00:00
abstract::A novel potassium channel beta-subunit (Kv beta 3) was cloned from rat brain being the third member of a Kv beta subunit gene family. It is a protein of 403 amino acid residues with a 68% amino acid sequence homology to Kv beta 1.1. Kv beta 3 is primarily expressed in rat brain having a distribution distinct to those ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01377-6
更新日期:1995-12-27 00:00:00
abstract::MicroRNA (miRNA) play a pivotal role in the development of liver fibrosis. However, the functions of miRNA in hepatitis C virus (HCV)-related liver fibrosis remain unclear. In this study, we systematically analyzed the microarray data of the serum miRNA in patients with HCV-induced hepatic fibrosis. Among 41 dysregula...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12309
更新日期:2016-08-01 00:00:00
abstract::Reconstitution of the 16 kDa N-terminal domain of the peridinin-chlorophyll-protein, N-PCP, with mixtures of chlorophyll a (Chl a) and Chl b, resulted in 32 kDa complexes containing two pigment clusters, each bound to one N-PCP. Besides homo-chlorophyllous complexes, hetero-chlorophyllous ones were obtained that conta...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.08.049
更新日期:2006-10-02 00:00:00
abstract::Two phosphoenolpyruvate carboxylase (PEPC) kinase genes (PPCk1 and PPCk2) are present in the Arabidopsis genome; only PPCk1 is expressed in rosette leaves. Homozygous lines of two independent PPCk1 T-DNA-insertional mutants showed very little (dln1), or no (csi8) light-induced PEPC phosphorylation and a clear retard i...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.04.030
更新日期:2009-05-19 00:00:00
abstract::Acaryochloris marina is a prochlorophyte-like cyanobacterium containing both phycobilins and chlorophyll d as light harvesting pigments. We show that the chlorophyll d light harvesting system, composed of Pcb proteins, functionally associates with the photosystem II (PSII) reaction center (RC) core to form a giant sup...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.01.023
更新日期:2005-02-14 00:00:00
abstract::A technique has been developed to probe directly RecA-DNA interactions by the use of the fluorescent chromophore, (+)anti-benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide (BPDE), covalently attached to DNA. The 24-mer oligonucleotide 5'-d(CTACTAAACATGTACAAATCATCC) was specifically modified on the exocyclic nitrogen of the ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00600-e
更新日期:1995-07-10 00:00:00
abstract::Protein kinase CK2 is composed of two regulatory beta-subunits and two catalytic alpha- or alpha'-subunits. To analyse these subunits individually we generated antibodies against unique peptides derived from the alpha-, alpha'- and beta-subunit. Immunofluorescence studies with these antibodies revealed the presence of...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01492-1
更新日期:1999-11-26 00:00:00
abstract::In contrast to the preponderance of affected males in families with X-linked mental retardation, Rett syndrome (RTT) is a neurological disorder occurring almost exclusively in females. The near complete absence of affected males in RTT families has been explained by the lethal effect of an X-linked gene mutation in he...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01994-3
更新日期:2000-09-22 00:00:00
abstract::Deuterated arachidonic acid (AA, [2H8]20:4 n-6) 1-25 microM, is converted to other fatty acids, as evaluated by gas chromatography-mass spectrometry, in THP-1 cells. The major products, in the 1 to 10 microM range, are 22:4 (elongated) and 20:3 (reduced in 5). At 25 microM, 24:4, 24:5 and 22:5 accumulate, with [2H8]/[...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)80554-7
更新日期:1994-05-02 00:00:00
abstract::Escherichia coli outer membrane protease OmpT has been characterised as a serine protease based on its inhibitor profile, but serine protease consensus sequences are absent. By site-directed mutagenesis we substituted all conserved serines and histidines. Substitution of His(101) and His(212) by Ala, Asn or Gln result...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01231-x
更新日期:2000-02-25 00:00:00
abstract::The plasma membrane (PM) sphingolipid composition is the result of a series of well-known metabolic pathways comprising neobiosynthesis in the endoplasmic reticulum and in the Golgi apparatus followed by vesicular delivery to the plasma membrane, membrane turnover with final catabolism in lysosomes, and shedding of me...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2009.11.020
更新日期:2010-05-03 00:00:00
abstract::Winter flounder contains two distinct anti-freeze protein isoforms, which are the liver-type extracellular anti-freeze proteins and the skin-type intracellular anti-freeze protein. The skin-type anti-freeze proteins exhibit lower anti-freeze activities than the liver-type isoforms and this might be due to their lackin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00752-8
更新日期:1999-06-25 00:00:00
abstract::Monoamine oxidase B (MAO B) is an outer mitochondrial membrane protein that oxidizes arylalkylamine neurotransmitters and has been a valuable drug target for many neurological disorders. The 1.7 angstrom resolution structure of human MAO B shows the enzyme is dimeric with a C-terminal transmembrane helix protruding fr...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/S0014-5793(04)00209-1
更新日期:2004-04-30 00:00:00
abstract::Mitochondrial thioredoxin (mtTrx) can be oxidized in response to inducers of oxidative stress; yet the functional consequences of the oxidation have not been determined. This study evaluated the redox status of mtTrx and its association to oxidant-induced apoptosis. Results showed that mtTrx was oxidized after exposur...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.11.007
更新日期:2006-12-11 00:00:00
abstract::Gap junction channels may be comprised of either connexin or pannexin proteins (innexins and pannexins). Membrane topologies of both families are similar, but sequence similarity is lacking. Recently, connexin-like sequences have been identified in mammalian and zebrafish genomes that have only four conserved cysteine...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.11.079
更新日期:2008-01-23 00:00:00
abstract::Vpu is an 81 amino acid protein encoded by HIV-1. Its role is to amplify viral release by two mechanisms: (i) docking to CD4 with the consequence of targeting CD4 for ubiquitine-mediated degradation, and (ii) formation of ion channels to enhance particle release. The intensive research on its in vivo function, combine...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/s0014-5793(03)00782-8
更新日期:2003-09-18 00:00:00
abstract::The dgd1 mutant of Arabidopsis thaliana provides us with a powerful tool for revealing the specific role of digalactosyldiacylglycerol (DGDG) in photosynthesis. Blue-native polyacrylamide gel electrophoresis analysis revealed that photosystem I (PSI) subunits are assembled into a PSI complex, and that a PSI subcomplex...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.05.049
更新日期:2005-07-04 00:00:00
abstract::Immunoprecipitated p100Gag-Mil protein kinase from MH2-transformed quail embryo fibroblasts is associated with an 89 kDa protein. The molar ratio between p89 and Gag-Mil in the immunocomplex is 0.72, indicating that the majority of Gag-Mil is complexed with p89. During mitosis part of Gag-Mil is shifted to a form with...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)80598-9
更新日期:1994-04-18 00:00:00
abstract::We have used a diethylpyrocarbonate (DEPC) modification [(1976) Prog. Nucl. Acids Res. 16, 189-262] to probe the accessibility of adenines essential for coat protein binding in the MS2 translational operator [(1983) Biochemistry 22, 2601-2610, 2610-2615, 4723-4730; (1987) Biochemistry 26, 1563-1568]. The essential ade...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80576-o
更新日期:1991-05-20 00:00:00
abstract::Schizosaccharomyces pombe cells take up D-gluconate, as an alternative carbon source for growth, during glucose starvation or when cultured on glycerol-containing medium. Gluconate uptake is not detectable while cells are growing logarithmically on glucose. The addition of D-glucose as well as its non-metabolizable an...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)01052-6
更新日期:1996-10-21 00:00:00
abstract::(-)-Epigallocatechin-3-O-gallate (EGCG) exhibits anti-tumor activity mediated via the 67-kDa laminin receptor (67LR). In this study, we found that 67LR protein levels are reduced by exposure to low O(2) levels (5%), without affecting the expression of HIF-1α. We also found that EGCG-induced anti-cancer activity is abr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.07.065
更新日期:2012-09-21 00:00:00
abstract::The degradation of type IX collagen, a minor collagen in cartilage, was examined by treatment with three different types of matrix metalloproteinases (MMPs) purified from the culture medium of rheumatoid synovial cells. Neither MMP-1 (collagenase) nor MMP-2 (so-called 'gelatinase') could digest type IX collagen, but M...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80586-1
更新日期:1989-02-27 00:00:00
abstract::Protein gene product 9.5 (PGP 9.5), which in the normal nervous system is restricted to certain neurons, has been detected in two glioma cell lines, rat C6 and human GL15, by immunoblotting and immunocytochemistry. Its expression in these cells depends on the cellular growth state, being maximal between the first and ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)81242-z
更新日期:1991-09-23 00:00:00
abstract::The freshwater ciliate Tetrahymena pyriformis is an ubiquitous organism that is present in all aquatic ecosystems. This protozoan showed a clear resistance against some polycyclic aromatic hydrocarbons which can be attributed to an efflux pump probably of the multidrug resistance (MDR) type. Immunocytochemical detecti...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00978-3
更新日期:1999-08-13 00:00:00
abstract::A synthetic form of human pancreatic growth hormone releasing factor (GRF-44-NH2) was shown to be a potent stimulator of growth hormone (GH) secretion and cellular cyclic AMP levels in cultured sheep pituitary cells. A small dose-dependent stimulation of prolactin secretion was also observed. Somatostatin (0.5 microM)...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80070-8
更新日期:1984-01-23 00:00:00
abstract::Sodium azide inhibited multi-site (steady-state) ATPase activity of E. coli F1 more than 90%, but did not affect uni-site (single-site) ATPase activity. Thus azide inhibited multi-site ATPase activity by lowering catalytic cooperativity. Consistent with this observation, azide changed the ligand-induced fluorescence r...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)81526-0
更新日期:1987-03-23 00:00:00