Investigating the mechanism of ADP-forming acetyl-CoA synthetase from the protozoan parasite Entamoeba histolytica.

abstract：:cDNA for a novel proton/organic cation transporter, OCTN1, was cloned from human fetal liver and its transport activity was investigated. OCTN1 encodes a 551-amino acid protein with 11 transmembrane domains and one nucleotide binding site motif. It is strongly expressed in kidney, trachea, bone marrow and fetal liver ...

journal_title：FEBS letters

authors： Tamai I,Yabuuchi H,Nezu J,Sai Y,Oku A,Shimane M,Tsuji A

更新日期：1997-12-08 00:00:00

abstract：:High-molecular-weight [14C]hyaluronate was incubated with cultured fibroblasts from human uterine cervix and skin, and then the depolymerization of the hyaluronate was investigated. [14C]Hyaluronate in the medium of skin fibroblasts was depolymerized into a constant molecular weight (M(r) about 40,000), whereas that o...

journal_title：FEBS letters

authors： Tanaka K,Nakamura T,Ikeya H,Higuchi T,Tanaka A,Morikawa A,Saito Y,Takagaki K,Endo M

更新日期：1994-06-20 00:00:00

abstract：:Okadaic acid is an inhibitor of the protein Ser/Thr phosphatases PP1 and PP2A, which blocks the activation of extracellular signal-regulated protein kinase 5 (ERK5), a member of the MAP kinase family activated by growth factors and several types of stressors. The blocking of ERK5 activation by okadaic acid was observe...

journal_title：FEBS letters

authors： Garcia L,Garcia F,Llorens F,Unzeta M,Itarte E,Gómez N

更新日期：2002-07-17 00:00:00

abstract：:In prokaryotic and eukaryotic organisms, proteins are efficiently sorted to reach their final destinations in a whole range of subcellular compartments. Targeting is mediated by hydrophobic signal sequences or hydrophilic targeting sequences depending upon the compartment, these sequences being often processed. Protei...

journal_title：FEBS letters

authors： Lazdunski CJ,Benedetti H

更新日期：1990-08-01 00:00:00

abstract：:Glycogen phosphorylase b at concentrations close to those found in skeletal muscle interacts with sarcoplasmic reticulum membranes, but not with liposomes made of lipids extracted from these membranes, and is inhibited upon binding to the membrane. The interaction of glycogen phosphorylase with the sarcoplasmic reticu...

journal_title：FEBS letters

authors： Cuenda A,Centeno F,Gutierrez-Merino C

更新日期：1991-06-03 00:00:00

abstract：:The Ca(2+)-activated photoprotein, aequorin, contains six tryptophan residues and has a bioluminescence emission maximum at 465 nm. On converting the six tryptophan residues to phenylalanine, the mutant aequorins exhibited varied luminescence activities and spectra, but one mutant, with tryptophan-86 replaced by pheny...

journal_title：FEBS letters

authors： Ohmiya Y,Ohashi M,Tsuji FI

更新日期：1992-04-20 00:00:00

abstract：:Signal recognition particle (SRP) interacts with the signal sequence in nascent secretory and membrane proteins and directs them to the membrane of the endoplasmic reticulum. Membrane targeting is mediated by the 68 and the 72 kDa proteins of SRP. We have cloned and sequenced cDNA encoding the 68 kDa protein of canine...

journal_title：FEBS letters

authors： Herz J,Flint N,Stanley K,Frank R,Dobberstein B

更新日期：1990-12-10 00:00:00

abstract：:Previous data have suggested an involvement of MDR/PGP-like ABC transporters in transport of the plant hormone auxin and, recently, AtPGP1 has been demonstrated to catalyze the primary active export of auxin. Here we show that related isoform AtPGP4 is expressed predominantly during early root development. AtPGP4 loss...

journal_title：FEBS letters

authors： Santelia D,Vincenzetti V,Azzarello E,Bovet L,Fukao Y,Düchtig P,Mancuso S,Martinoia E,Geisler M

更新日期：2005-10-10 00:00:00

abstract：:Prostaglandin E2 receptors (EPR), which belong to the family of heterotrimeric G protein-coupled ectoreceptors with seven transmembrane domains, can be classified into four subtypes according to their ligand binding and G protein coupling specificity. Of these, EP3betaR is coupled to Gi, whereas EP4R is coupled to Gs....

journal_title：FEBS letters

authors： Neuschäfer-Rube F,Hänecke K,Püschel GP

更新日期：1997-09-29 00:00:00

abstract：:The reduction of methyl-coenzyme M (CH3SCoM) to methane in methanogenic bacteria is dependent on component B (N-7-mercaptoheptanoyl-O-phospho-L-threonine, HSHTP). We report here that S-methyl-component B (N-7-(methylthio)heptanoyl-O-phospho-L-threonine, CH3SHTP) can substitute for neither CH3SCoM nor HSHTP in the meth...

journal_title：FEBS letters

authors： Ellermann J,Kobelt A,Pfaltz A,Thauer RK

更新日期：1987-08-17 00:00:00

abstract：:The molecular chaperone protein Hsp78, a member of the Clp/Hsp100 family localized in the mitochondria of Saccharomyces cerevisiae, is required for maintenance of mitochondrial functions under heat stress. To characterize the biochemical mechanisms of Hsp78 function, Hsp78 was purified to homogeneity and its role in t...

journal_title：FEBS letters

authors： Krzewska J,Langer T,Liberek K

更新日期：2001-01-26 00:00:00

abstract：:The oxidoreductase ERp57 is a component of the major histocompatibility complex (MHC) class I peptide-loading complex. ERp57 can interact directly with MHC class I molecules, however, little is known about which of the cysteine residues within the MHC class I molecule are relevant to this interaction. MHC class I mole...

journal_title：FEBS letters

authors： Antoniou AN,Santos SG,Campbell EC,Lynch S,Arosa FA,Powis SJ

更新日期：2007-05-15 00:00:00

abstract：:In the phylogeny of plant polyamine oxidases (PAOs), clade III members from angiosperms, such as Arabidopsis thaliana PAO5 and Oryza sativa PAO1, prefer spermine and thermospermine as substrates and back-convert both of these substrates to spermidine in vitro. A clade III representative of lycophytes, SelPAO5 from Sel...

journal_title：FEBS letters

authors： Sagor GH,Inoue M,Kim DW,Kojima S,Niitsu M,Berberich T,Kusano T

更新日期：2015-10-07 00:00:00

abstract：:The role of retinoic acids (RA) on liver fatty acid-binding protein (L-FABP) expression was investigated in the well differentiated FAO rat hepatoma cell line. 9-cis-Retinoic acid (9-cis-RA) specifically enhanced L-FABP mRNA levels in a time- and dose-dependent manner. The higher induction was found 6 h after addition...

journal_title：FEBS letters

authors： Poirier H,Braissant O,Niot I,Wahli W,Besnard P

更新日期：1997-08-04 00:00:00

abstract：:The complete amino acid sequence of porcine muscarinic acetylcholine receptor III has been deduced by cloning and sequencing the genomic DNA. The antagonist binding properties of muscarinic acetylcholine receptor III expressed from the cloned DNA in Xenopus oocytes correspond most closely to those of the pharmacologic...

journal_title：FEBS letters

authors： Akiba I,Kubo T,Maeda A,Bujo H,Nakai J,Mishina M,Numa S

更新日期：1988-08-01 00:00:00

abstract：:All acetylcholine receptor subunit genes contain E boxes and are blocked by membrane depolarization. We have used transfected C2C12 myogenic cells to investigate the response, to electrical stimulation and KCl, of wildtype and mutant regulatory regions of the chick acetylcholine receptor alpha, gamma and delta subunit...

journal_title：FEBS letters

authors： Su CT,Huang CF,Schmidt J

更新日期：1995-06-12 00:00:00

abstract：:Hepatitis C virus (HCV) replication and pathogenesis involve both virus-encoded proteins and cellular factors. In our study, we showed that NS5B, the HCV RNA-dependent RNA polymerase, interacted with M2 type pyruvate kinase (M2PK) but not L type pyruvate kinase. We confirmed the interaction by GST pull down, coimmunop...

journal_title：FEBS letters

authors： Wu X,Zhou Y,Zhang K,Liu Q,Guo D

更新日期：2008-06-25 00:00:00

abstract：:DNA methylation has a profound impact on the regulation of gene expression in normal cell development, and aberrant methylation has been recognized as a key factor in the pathogenesis of human diseases such as cancer. The discovery of modified nucleobases arising from 5-methylcytosine (5mC) through consecutive oxidati...

journal_title：FEBS letters

authors： Dietzsch J,Feineis D,Höbartner C

更新日期：2018-06-01 00:00:00

abstract：:The CO-binding reaction of cytochrome P-450cam bound with (1R)-camphor and (1S)-camphor are compared in the temperature region of 210-260 K using time-resolved Fourier-transform infrared spectroscopy with the CO stretch vibration as spectroscopic probe. For (1S)-camphor as substrate the association of CO is slowed dow...

journal_title：FEBS letters

authors： Contzen J,Ristau O,Jung C

更新日期：1996-03-25 00:00:00

abstract：:Haloalkane dehalogenase hydrolyses various 1-halo-n-alkanes to the corresponding alcohols by covalent catalysis with formation of an alkyl-enzyme intermediate. The carboxylate function of the nucleophilic aspartate (Asp-124) that displaces the halogen during formation of the intermediate was changed to an amide by sit...

journal_title：FEBS letters

authors： Pries F,Kingma J,Janssen DB

更新日期：1995-01-23 00:00:00

abstract：:An open reading frame encoding a putative polypeptide very similar to several lysyl-tRNA synthetases was found 10 nucleotides downstream of Rhizobium meliloti gltX encoding glutamyl-tRNA synthetase. Expression of this gene complemented a mutation in lysS of Escherichia coli and led to the overexpression of a polypepti...

journal_title：FEBS letters

authors： Pelchat M,Gagnon Y,Laberge S,Lapointe J

更新日期：1999-04-16 00:00:00

abstract：:Revised assignments for the bilayer helices of the beta-, gamma- and delta-subunits of the acetylcholine receptor are presented. A new feature of the model is extensive charge matching between the polar groups of the ion channel elements of different subunits. ...

journal_title：FEBS letters

authors： Kosower EM

更新日期：1984-06-25 00:00:00

abstract：:The MDM2 oncoprotein is overexpressed in many human tumors and cancers. MDM2 functions as an E3 ligase for p53 and for itself. MDM2 also interacts with the retinoblastoma protein (RB) and the transcription factor E2F1 to promote cell cycle S-phase entry. Here, we report that MDM2 protein expression is cell cycle-regul...

journal_title：FEBS letters

authors： Gu L,Ying H,Zheng H,Murray SA,Xiao ZX

更新日期：2003-06-05 00:00:00

abstract：:Large conductance voltage-dependent and Ca(2+)-modulated K(+) channels play a crucial role in myometrium contractility. Western blots and immunocytochemistry of rat uterine sections or isolated cells show that MaxiK channel protein signals drastically decrease towards the end of pregnancy. Consistent with a transcript...

journal_title：FEBS letters

authors： Song M,Zhu N,Olcese R,Barila B,Toro L,Stefani E

更新日期：1999-11-05 00:00:00

abstract：:We show that inverted membrane vesicles from Corynebacterium glutamicum, a Gram-positive bacterium, are able to generate and maintain an electrochemical gradient of protons in response to the addition of NADH. This result indicates that the respiratory chain is intact and that the vesicles are reasonably impermeable t...

journal_title：FEBS letters

authors： Schrempp S,Bayan N,Shechter E

更新日期：1994-12-12 00:00:00

abstract：:Regulators of G protein signaling RGS4 and RGS7 accelerate the kinetics of K(+) channels (GIRKs) in the Xenopus oocyte system. Here, via quantitative analysis of RGS expression, we reveal biphasic effects of RGSs on GIRK regulation. At low concentrations, RGS4 inhibited basal GIRK activity, but stimulated it at high c...

journal_title：FEBS letters

authors： Keren-Raifman T,Bera AK,Zveig D,Peleg S,Witherow DS,Slepak VZ,Dascal N

更新日期：2001-03-09 00:00:00

abstract：:Expression of androgen receptor (AR) in prostate epithelial cells is thought to regulate cell proliferation, differentiation, and survival. However, the molecular mechanisms remain unclear. We report that re-expression of AR in PC-3 human prostate cancer cell line resulted in upregulation of IFI16 protein, a negative ...

journal_title：FEBS letters

authors： Alimirah F,Chen J,Xin H,Choubey D

更新日期：2006-03-06 00:00:00

abstract：:Temperature-induced order/disorder transition profiles were obtained from the membranes of intact Gram-negative bacterial cells by FT-IR analysis of the frequency shifts of the acyl chain methylene symmetric stretching band as a monitor. Cells grown at different temperatures yielded distinct transition profiles. At th...

journal_title：FEBS letters

authors： Schultz C,Naumann D

更新日期：1991-12-02 00:00:00

abstract：:Binding of the thiazolyl peptide antibiotic thiostrepton to the GTPase domain of 23S rRNA involves a few crucial nucleotides, notably A1067 (E. coli). Small RNA transcripts were prepared corresponding to the GTPase domain of the plastid 23S rRNA and the two forms of cytosolic 28S rRNAs found in the human malaria paras...

journal_title：FEBS letters

authors： Clough B,Strath M,Preiser P,Denny P,Wilson IR

更新日期：1997-04-07 00:00:00

abstract：:The Rad51 gene of Saccharomyces cerevisiae is required for genetic recombination and recombinational repair of DNA strand breaks. In higher eukaryotes Rad51 is essential for embryonic development, and is involved in cell proliferation and DNA repair. Here we show that human Rad51 (HsRad51) is proteolytically cleaved d...

journal_title：FEBS letters

authors： Flygare J,Armstrong RC,Wennborg A,Orsan S,Hellgren D

更新日期：1998-05-08 00:00:00