Abstract:
:The effect on cytosolic Ca2+ concentration ([Ca2+]i) of cAMP analogues and the adenylate cyclase-stimulating agents forskolin, isoproterenol and glucagon has been examined in an insulin-secreting beta-cell line (HIT T-15) using fura 2. All these manipulations of the cAMP messenger system promoted a rise in [Ca2+]i which was blocked by the Ca2+ channel antagonists verapamil and nifedipine or by removal of extracellular Ca2+. The action of the adenylate cyclase activator forskolin was glucose-dependent. The results suggest that cAMP elevates [Ca2+]i in HIT cells by promoting Ca2+ entry through voltage-sensitive Ca2+ channels, not through mobilization of stored Ca2+. Activation of Ca2+ influx may be an important component of the mechanisms by which cAMP potentiates fuel-induced insulin release.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Prentki M,Glennon MC,Geschwind JF,Matschinsky FM,Corkey BEdoi
10.1016/0014-5793(87)80884-0subject
Has Abstractpub_date
1987-08-10 00:00:00pages
103-7issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(87)80884-0journal_volume
220pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Bacterial cells containing the ner gene of phage Mu inserted into pBR322 express a binding activity with specificity for the left-end EcoRI.C fragment of Mu DNA. Crude extracts containing either Mu repressor or ner protein have been used to localize binding sites on TaqI subfragments of the EcoRI.C fragment. There are...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)81128-4
更新日期:1983-06-27 00:00:00
abstract::Piriprost and nordihydroguiaretic acid (NDGA), specific inhibitors of arachidonate lipoxygenase, inhibited phytohaemagglutinin (PHA)-stimulated breakdown of inositol lipids in human T lymphocytes. The dual inhibitors eicosatetraynoic acid (ETYA) and BW 755C, which inhibit both lipoxygenase and cyclooxygenase, also had...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81621-7
更新日期:1989-11-20 00:00:00
abstract::Polypeptides rich in glutamic acid are strong inhibitors purified from isolated nuclei of Xenopus laevis oocytes of casein kinase II. The presence of tyrosine in these peptides greatly enhances their inhibitory capacity. Using casein as a substrate, copolyglu:tyr (4:1) has an I50 value of 20 nM, 250 fold lower than th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80897-r
更新日期:1990-06-04 00:00:00
abstract::Glycogen storage disease type Ia (GSD-Ia) patients manifest a pro-atherogenic lipid profile but are not at elevated risk for developing atherosclerosis. Serum phospholipid, which correlates positively with the scavenger receptor class B type I (SR-BI)-mediated cholesterol efflux, and apolipoprotein A-IV and E, accepto...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.07.042
更新日期:2005-08-29 00:00:00
abstract::Fatty acid acylation of membrane proteins was studied on human erythrocytes by measuring incorporation of [3H]palmitate at different specific radioactivities. A 55 kDa polypeptide within the band 4.5 region was the main acceptor protein for acylation by fatty acids (palmitate, stearate, oleate), while other polypeptid...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80033-f
更新日期:1990-01-01 00:00:00
abstract::Proteasomes were purified from human hepatoma tissues, and their sensitivities to Na+ and K+ were examined. At concentrations of 10 mM or more, these cations were found to inhibit completely polylysine-activated casein degradation by the purified proteasomes. They also strongly inhibited the hydrolyses of peptides, al...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81333-x
更新日期:1989-04-24 00:00:00
abstract::Mammalian heart has been reported to express AC isozymes (types V and VI) that are inhibited by < microM [Ca2+]; avian heart has been reported to express adenylyl cyclase activity that is inhibited by < microM [Ca2]. We have used reverse transcription polymerase chain reaction (RT-PCR) to determine that type V and VI ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01084-r
更新日期:1995-10-23 00:00:00
abstract::The involvement of reactive oxygen species in Ca(2+)-induced mitochondrial membrane permeabilization and cell viability was studied using yeast cells in which the thioredoxin peroxidase (TPx) gene was disrupted and/or catalase was inhibited by 3-amino-1,2, 4-triazole (ATZ) treatment. Wild-type Saccharomyces cerevisiae...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01526-x
更新日期:2000-05-12 00:00:00
abstract::The SEC10 gene product is a member of the Exocyst complex essential for exocytosis in the budding yeast Saccharomyces cerevisiae. We report here the cloning and characterization of human Sec10p (hSec10p; GenBank accession number U85946). hSec10p is a 77-kDa protein with 23% amino acid identity to yeast Sec10p and 37% ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00109-9
更新日期:1997-03-10 00:00:00
abstract::Oligonucleotide probes based on the known amino acid sequence of salmon calcitonin were used to screen a cDNA library obtained from ultimobranchial glands of salmon for clones encoding salmon calcitonin. From the cDNA sequence of strongly hybridizing clones the complete primary structure of the calcitonin precursor co...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80558-6
更新日期:1987-12-21 00:00:00
abstract::Recent advances in our understanding of the structure of highly amplified DNA sequences in Streptomyces fradiae and lividans have enabled us to formulate a possible mechanism by which amplification may occur. An essential feature of the model is the generation of an amplification precursor, which comprises a circulari...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)81547-8
更新日期:1987-02-09 00:00:00
abstract::Drosophila PTEN (dPTEN) plays indispensable roles in the development of Drosophila melanogaster by controlling cell size and number. Although three potential spliced forms of dPTEN have been isolated, functional distinction among these forms remains elusive. In this study, we demonstrate that all spliced forms of dPTE...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.03.074
更新日期:2004-05-07 00:00:00
abstract::14-3-3 proteins were the first signaling molecules to be identified as discrete phosphoserine/threonine binding modules. This family of proteins, which includes seven isotypes in human cells and up to 15 in plants, plays critical roles in cell signaling events that control progress through the cell cycle, transcriptio...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/s0014-5793(01)03288-4
更新日期:2002-02-20 00:00:00
abstract::Using the patch-clamp technique, we demonstrate here the opening of K+ channels evoked by the actively transported amino acid L-alanine in isolated Necturus enterocytes. These channels had a conductance of about 30 pS and their activation was dependent on transmembrane electrical potential and cytosolic Ca2+. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80134-0
更新日期:1988-07-18 00:00:00
abstract::Fibroblast growth factor 11 (FGF11) is an intracellular FGF. Although induction of FGF11 by hypoxia has been observed in several cell types, the molecular function of FGF11 is not clearly understood yet. Here, we investigated the role of FGF11 under hypoxia. We identified hypoxia-inducible factor-1α (HIF-1α) as an int...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12547
更新日期:2017-01-01 00:00:00
abstract::Phosphorylation of the 20 kDa myosin light chain from smooth muscle by five different kinases was investigated. Three of the kinases (myosin light chain kinase, phosphorylase kinase, and cAMP-dependent protein kinase) phosphorylate serine residues, the fourth (casein-kinase-2) mainly threonine, and the fifth (glycogen...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80449-9
更新日期:1983-08-08 00:00:00
abstract::Retroviruses have been etiologically implicated with leukemia in humans and animals. Understanding the virus life cycle, the proteins and enzymes involved in its replication, is essential for developing potent anti-viral drugs. Phosphorylation of retroviral proteins may alter their shape in such a way as to increase o...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00820-4
更新日期:1996-09-02 00:00:00
abstract::In order to identify physiological activators of proteinase-activated receptor-2 (PAR-2), a peptide chloromethane inhibitor (biotinyl-Ser-Lys-Gly-Arg-CH2Cl) based on the cleavage site for activation of PAR-2 was synthesised and tested with 12 trypsin-like serine proteinases. The second-order rate constant (ki/Ki) for ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01298-2
更新日期:1997-11-17 00:00:00
abstract::NADP-malic enzyme (NADP-ME) is a widely distributed enzyme that catalyzes the oxidative decarboxylation of L-malate. Photosynthetic NADP-MEs are found in C4 bundle sheath chloroplasts and in the cytosol of CAM plants, while non-photosynthetic NADP-MEs are either plastidic or cytosolic in various plants. We propose a c...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/s0014-5793(00)02331-0
更新日期:2001-02-09 00:00:00
abstract::The Methanocaldococcus jannaschii (formerly Methanococcus jannaschii) protein Mj0968 has been reported to represent a soluble P-type ATPase [Ogawa et al., FEBS Lett. 471 (2000) 99-102]. In this study, we report that the heterologously expressed Mj0968-His(10) protein exhibits high rates of phosphatase activity, wherea...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00372-7
更新日期:2003-05-22 00:00:00
abstract::The antigenic cross-reactivity between purified chick, eel and mouse electrolectins (endogenous beta-D-galactoside specific lectins) have been studied using a solid phase radioimmunoassay. The immune serum raised against the eel electrolectin crossreacts both with the chick and the mouse electrolectins, while the anti...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(82)81261-1
更新日期:1982-11-01 00:00:00
abstract::The vasopressin precursor is composed of 3 domains in line, namely vasopressin, MSEL-neurophysin and a glycopeptide referred to as copeptin, which are separated during the processing. In guinea pig neurohypophysis, the precursor is partially processed so that a two-domain fragment, MSEL-neurophysin--copeptin, can be f...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80320-9
更新日期:1986-03-03 00:00:00
abstract::Mild sonication of eukaryotic cells produces native plasma membrane sheets that retain their docked organelles, cytoskeleton structures and cytoplasmic complexes. While the delicate organization of membranous protein complexes remains undisturbed, their inner plasmalemmel leaflet can be rapidly exposed to bathing solu...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.08.040
更新日期:2008-10-15 00:00:00
abstract::An approach is presented for the determination of the branch location of 1 or 2 extra N-acetyllactosamine units in sialo N-linked carbohydrate chains from glycoproteins. Tetraantennary oligosaccharides containing extra N-acetyllactosamine units were digested with endo-beta-galactosidase, followed by treatment with N-a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80931-r
更新日期:1991-07-29 00:00:00
abstract::RNA-binding protein kinase from amphibian oocytes modifies serine and threonine residues in the molecules of substrates and utilizes both ATP and GTP. Low concentrations of heparin inhibit protein kinase. The foregoing suggests that this enzyme is casein kinase II. It is shown that RNA-binding proteins lack active for...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)81363-0
更新日期:1984-05-07 00:00:00
abstract::The carboxy-terminal half of the c-src protein fused to the protein A moiety was expressed in bacteria. The protein A/truncated c-src fusion protein, which does not have SH2 and SH3 domains, is found in the periplasmic space allowing for a simple one-step purification and demonstrated high efficiency in autophosphoryl...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80174-s
更新日期:1993-07-26 00:00:00
abstract::ADP-forming acetyl-CoA synthetase (ACD) catalyzes the interconversion of acetyl-CoA and acetate. The related succinyl-CoA synthetase follows a three-step mechanism involving a single phosphoenzyme, but a novel four-step mechanism with two phosphoenzyme intermediates was proposed for Pyrococcus ACD. Characterization of...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.12573
更新日期:2017-02-01 00:00:00
abstract::Heparin Binding Hemagglutinin A (HBHA) is hitherto the sole virulence factor associated with tuberculosis dissemination from the lungs, the site of primary infection, to epithelial cells. We have previously reported the solution structure of HBHA, a dimeric and elongated molecule. Since oligomerisation of HBHA is asso...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.02.044
更新日期:2010-03-19 00:00:00
abstract::DNA is very stable in its double-stranded form. For many processes of DNA metabolism, such as replication, repair, recombination and transcription, the DNA has to be brought transiently into a single-stranded form. DNA helicases are enzymes capable of melting the hydrogen bonds of base pairs by using the energy of nuc...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/0014-5793(90)81279-w
更新日期:1990-08-01 00:00:00
abstract::We previously reported that IκBL prevents experimental autoimmune arthritis. The molecular mechanism, however, still remains unclear. In contrast to four splicing-isoforms of IκBL in human, two isoforms were identified in mouse. The major isoform IκBL-α(S) suppressed LPS-induced NF-κB activation and transcription of T...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.10.024
更新日期:2011-11-16 00:00:00