Identification of amino acid residues of Ras protein that are essential for signal-transducing activity but not for enhancement of GTPase activity by GAP.

abstract：:We present the first X-ray study of a member of the arrestin family, the bovine retinal arrestin. Arrestin is essential for the fine regulation and termination of the light-induced enzyme cascade in vertebrate rod outer segments. It plays an important role in quenching phototransduction by its ability to preferentiall...

journal_title：FEBS letters

authors： Wilden U,Choe HW,Krafft B,Granzin J

更新日期：1997-10-06 00:00:00

abstract：:The incorporation of carbon into the carbon monoxide and cyanide ligands of [NiFe]-hydrogenases has been investigated by using (13)C labelling in infrared studies of the Allochromatium vinosum enzyme and by (14)C labelling experiments with overproduced Hyp proteins from Escherichia coli. The results suggest that the b...

journal_title：FEBS letters

authors： Roseboom W,Blokesch M,Böck A,Albracht SP

更新日期：2005-01-17 00:00:00

abstract：:We identified a gene encoding a novel secreted protein in mice, humans, and zebrafish. As the protein of 222 amino acids is similar to Brorin, a secreted BMP antagonist, which is a member of the Chordin family, we named it Brorin-like. Recombinant Brorin-like protein weakly but significantly inhibited the activity of ...

journal_title：FEBS letters

authors： Miwa H,Miyake A,Kouta Y,Shimada A,Yamashita Y,Nakayama Y,Yamauchi H,Konishi M,Itoh N

更新日期：2009-11-19 00:00:00

abstract：:The Pb, Eu and Mg-induced cleavages in E. coli and lupine tRNAPhe have been characterized and compared with those found in yeast tRNAPhe. The pattern of lupine tRNAPhe hydrolysis closely resembles that of yeast tRNAPhe, while several major differences occur in the specificity and efficiency of the E. coli tRNAPhe hydr...

journal_title：FEBS letters

authors： Marciniec T,Ciesiołka J,Wrzesinski J,Krzyzosiak WJ

更新日期：1989-01-30 00:00:00

abstract：:The aim of the present study is to determine the role of intracellular Ca(2+) in VEGF signaling. We demonstrate that reduction in Ca(2+) by chelating compound BAPTA-AM or by IP(3)-endoplasmic reticulum blocker 2-APB selectively inhibited VEGF-induced activation of c-Src-PI3K-Akt but not ERK1/2 in human coronary artery...

journal_title：FEBS letters

authors： Lee M,Spokes KC,Aird WC,Abid MR

更新日期：2010-07-16 00:00:00

abstract：:Exposure of Ehrlich ascites tumor cells to 5-azacytidine for 5 h resulted in a partial loss of ability of DNA to stimulate ADP-ribosyltransferase activity, as assessed in a reconstituted in vitro enzyme system consisting of purified calf thymus enzyme, calf thymus whole histone and DNA isolated from the cells. The deg...

journal_title：FEBS letters

authors： Hoshino J,Nothacker HP,Schalge S,Kröger H

更新日期：1990-03-12 00:00:00

abstract：:Tropomyosins can be classified as belonging to an alpha-like or a beta-like family depending on the absence or presence, respectively in their protease-V8 digestion pattern of two peptides with an apparent molecular mass of 21 kDa. Chicken cardiac tropomyosin and the 43 kDa component from gizzard tropomyosin are accor...

journal_title：FEBS letters

authors： Kardami E,Fiszman MY

更新日期：1983-11-14 00:00:00

abstract：:Systemic AA-amyloidosis is a complication of chronic inflammatory diseases and the fibril protein AA derives from the acute phase reactant serum AA. AA-amyloidosis can be induced in mice by an inflammatory challenge. The lag phase before amyloid develops can be dramatically shortened by administration of a small amoun...

journal_title：FEBS letters

authors： Westermark GT,Westermark P

更新日期：2009-08-20 00:00:00

abstract：:The induction of apoptosis by cell cycle regulator molecules under conditions optimal for exponential growth was examined in rat pheochromocytoma PC12 cells by overexpression of cyclins and cyclin-dependent kinases (cdks). By flow cytometry and by immunofluorescence, only cells overexpressing cdk4 or cyclin D1 underwe...

journal_title：FEBS letters

authors： Katayama K,Dobashi Y,Kitagawa M,Kamekura S,Kawai M,Kadoya Y,Kameya T

更新日期：2001-12-14 00:00:00

abstract：:Growth of E. coli in the presence of the protonophorous uncoupler pentachlorophenol is shown to strongly enhance levels of cytochrome d, a putative Na(+)-motive oxidase. This effect was found to be arrested by chloramphenicol and stimulated by high Na+ concentration in the growth medium. The induction of cytochrome d ...

journal_title：FEBS letters

authors： Bogachev AV,Murtazina RA,Skulachev VP

更新日期：1993-12-20 00:00:00

abstract：:The (beta alpha)(8)-barrel is the most frequent and most versatile fold among enzymes [Höcker et al., Curr. Opin. Biotechnol. 12 (2001) 376-381; Wierenga, FEBS Lett. 492 (2001) 193-198]. Structural and functional evidence suggests that (beta alpha)(8)-barrels evolved from an ancestral half-barrel, which consisted of f...

journal_title：FEBS letters

authors： Höcker B,Schmidt S,Sterner R

更新日期：2002-01-16 00:00:00

abstract：:In isolated rat liver perfused at constant flow with erythrocyte-free Krebs-Henseleit bicarbonate buffer containing 5 mM glucose and 2 mM lactate, perivascular stimulation of the hepatic nerves caused a rapid decrease of oxygen uptake, a decrease of the periportal and, after a transient rise, of the perivenous tissue ...

journal_title：FEBS letters

authors： Ji S,Beckh K,Jungermann K

更新日期：1984-02-13 00:00:00

abstract：:Yeasts with disruptions in the genes PYC1 and PYC2 encoding the isoenzymes of pyruvate carboxylase cannot grow in a glucose-ammonium medium (Stucka et al. (1991) Mol. Gen. Genet. 229, 307-315). We have isolated a dominant mutation, BPC1-1, that allows growth in this medium of yeasts with interrupted PYC1 and PYC2 gene...

journal_title：FEBS letters

authors： Blázquez MA,Gamo FJ,Gancedo C

更新日期：1995-12-18 00:00:00

abstract：:Saccharomyces cerevisiae Vig4/Vrg4 protein is a Golgi membrane protein which has multiple transmembrane domains and is essential for transport of GDP-mannose across the Golgi membrane. By immunoprecipitation of detergent-solubilized tagged protein, we found that this protein exists as oligomer. Two mutants vig4-1 and ...

journal_title：FEBS letters

authors： Abe M,Hashimoto H,Yoda K

更新日期：1999-09-24 00:00:00

abstract：:Contribution of phosphatidic acid (PA) generated by activated phospholipase (PL) D to PLA2 activation was studied in rat peritoneal mast cells. Exogenous didecanoyl PA induced arachidonate liberation in the permeabilized cells which was inhibited by p-bromophenacyl bromide. Upon exposure of the cells to ethanol in a h...

journal_title：FEBS letters

authors： Sato T,Ishimoto T,Akiba S,Fujii T

更新日期：1993-05-24 00:00:00

abstract：:The rapid conversion of the carotenoid violaxanthin to zeaxanthin via antheraxanthin (xanthophyll cycle) in potato leaves exposed at 23 degrees C to a strong white light of 2000 microE.m-2.s-1 was associated with a slight inhibition of photosynthetic electron transport (as estimated from chlorophyll fluorescence measu...

journal_title：FEBS letters

authors： Sarry JE,Montillet JL,Sauvaire Y,Havaux M

更新日期：1994-10-17 00:00:00

abstract：:Dimethylsulphoxide (DMSO) reductase from R. capsulatus contains a molybdenum-pterin cofactor at its active site. As prepared the molybdenum is in the 6+ oxidation state, devoid of EPR signals. Stepwise reduction generates an EPR signal characteristic of Mo(V) having hyperfine coupling to a single proton and integratin...

journal_title：FEBS letters

authors： Benson N,Farrar JA,McEwan AG,Thomson AJ

更新日期：1992-07-28 00:00:00

abstract：:Regulatory divergence is likely a major driving force in evolution. Comparative genomics is being increasingly used to infer the evolution of gene regulation. Ascomycota fungi are uniquely suited among eukaryotes for regulatory evolution studies, due to broad phylogenetic scope, many sequenced genomes, and tractabilit...

journal_title：FEBS letters

authors： Thompson DA,Regev A

更新日期：2009-12-17 00:00:00

abstract：:A new enzyme catalyzing the deamidation of seed storage proteins was found in germinating wheat grains and was partially purified. It also acts on egg lysozyme, horse hemoglobin and reduced RNAse, glutamine and Gly-L-Gln-L-Tyr. No activity was observed when using ovalbumin, serum albumin, RNAse, insulin, asparagine an...

journal_title：FEBS letters

authors： Vaintraub IA,Kotova LV,Shaha R

更新日期：1992-05-11 00:00:00

abstract：:Using PCR-based techniques, we have isolated and sequenced the full-length cDNAs that encode the human neuronal nAChR subunits alpha3,4,5,7 and beta2,3,4 in the neuroblastoma cell lines SH-SY5Y and/or IMR-32. The predicted nAChR beta3- and beta4-subunit proteins contain 458 and 498 amino acids, respectively. Except fo...

journal_title：FEBS letters

authors： Groot Kormelink PJ,Luyten WH

更新日期：1997-01-06 00:00:00

abstract：:We have measured SGLT1 mRNA content and SGLT1-mediated glucose transport at different positions along the small intestine of control and streptozotocin diabetic rats and shown both parameters to be similar but higher in jejunal compared with ileal tissue. No such correlation was seen when comparing measurements of SGL...

journal_title：FEBS letters

authors： Dong R,Srai SK,Debnam E,Smith M

更新日期：1997-04-07 00:00:00

abstract：:We have identified in rat vascular smooth muscle cells (SMCs) the simultaneous expression of two TGF-beta type I receptor (ALK-5) cDNAs, occurring as a consequence of alternate usage of AG splice acceptor motifs separated by 12 nucleotides located at an intron-exon junction. When translated the resultant full length p...

journal_title：FEBS letters

authors： Agrotis A,Condron M,Bobik A

更新日期：2000-02-04 00:00:00

abstract：:A cDNA of a novel form of type V adenylyl cyclase has been cloned from rabbit myocardium using oligonucleotide probes derived from peptides that were produced by enzymatic cleavage of purified heart cyclase. A corresponding mRNA (6 kb) has been detected in rabbit myocardial tissue by Northern blot analysis. The cDNA e...

journal_title：FEBS letters

authors： Wallach J,Droste M,Kluxen FW,Pfeuffer T,Frank R

更新日期：1994-02-07 00:00:00

abstract：:Stopped-flow kinetics showed that the inhibition of the lysosomal cysteine proteinase, cathepsin B, by its endogenous inhibitor, cystatin C, occurs by a two-step mechanism, in which an initial, weak interaction is followed by a conformational change. The initial interaction most likely involves binding of the N-termin...

journal_title：FEBS letters

authors： Nycander M,Estrada S,Mort JS,Abrahamson M,Björk I

更新日期：1998-01-23 00:00:00

abstract：:Zinc metallopeptidases that contain the His-Glu-Xaa-Xaa-His (HEXXH) motif generally have a third ligand of the metal ion that may be either a Glu residue (in clan MA) or a His residue (in clan MB) (Rawlings and Barrett (1995) Methods Enzymol. 248, 183-228). Thimet oligopeptidase has not yet been assigned to either cla...

journal_title：FEBS letters

authors： Chen JM,Stevens RA,Wray PW,Rawlings ND,Barrett AJ

更新日期：1998-09-11 00:00:00

abstract：:The effect on cytosolic Ca2+ concentration ([Ca2+]i) of cAMP analogues and the adenylate cyclase-stimulating agents forskolin, isoproterenol and glucagon has been examined in an insulin-secreting beta-cell line (HIT T-15) using fura 2. All these manipulations of the cAMP messenger system promoted a rise in [Ca2+]i whi...

journal_title：FEBS letters

authors： Prentki M,Glennon MC,Geschwind JF,Matschinsky FM,Corkey BE

更新日期：1987-08-10 00:00:00

abstract：:The glycine-alanine (GA) repeat of the Epstein-Barr virus nuclear antigen-1 inhibits in cis ubiquitin-dependent proteolysis in mammalian cells through a yet unknown mechanism. In the present study we demonstrate that the GA repeat targets an evolutionarily conserved step in proteolysis since it can prevent the degrada...

journal_title：FEBS letters

authors： Heessen S,Dantuma NP,Tessarz P,Jellne M,Masucci MG

更新日期：2003-12-04 00:00:00

abstract：:Apoptotic proteases cleave and inactivate survival signaling molecules such as Akt/PKB, phospholipase C (PLC)-gamma1, and Bcl-2. We have found that treatment of A431 cells with tumor necrosis factor-alpha in the presence of cycloheximide resulted in the cleavage of epidermal growth factor receptor (EGFR) as well as th...

journal_title：FEBS letters

authors： Bae SS,Choi JH,Oh YS,Perry DK,Ryu SH,Suh PG

更新日期：2001-02-23 00:00:00

abstract：:Undifferentiated rat pheochromocytoma PC12 cells were voltage clamped using the whole cell technique. After blockade of outward currents, calcium currents were elicited from -40 and -100 mV. A subpopulation of cells displayed only one current component activated at -10 mV and slowly decaying. In other cells this curre...

journal_title：FEBS letters

authors： Janigro D,Maccaferri G,Meldolesi J

更新日期：1989-09-25 00:00:00

abstract：:Transport of the precursor for carbamoyl-phosphate synthetase I into mitochondria in Reuber hepatoma H-35 cells was inhibited by adding monensin or nigericin to the culture medium at a concentration of 0.5 microM, and the enzyme precursor accumulated, mainly in the cytosolic fraction. Accumulated precursor was degrade...

journal_title：FEBS letters

authors： Kitagawa Y,Murakami A,Sugimoto E

更新日期：1984-01-02 00:00:00