Abstract:
:Exposure of Ehrlich ascites tumor cells to 5-azacytidine for 5 h resulted in a partial loss of ability of DNA to stimulate ADP-ribosyltransferase activity, as assessed in a reconstituted in vitro enzyme system consisting of purified calf thymus enzyme, calf thymus whole histone and DNA isolated from the cells. The degree of suppression in vitro varied depending on the amount of histone and DNA added and it reached a maximum with a value of 83% and 62% of control for DNAs from cells exposed to 10 microM and 30 microM 5-azacytidine, respectively, at a histone/DNA mass ratio of 0.4. In the absence of histone (conditions of auto-ADP-ribosylation of the enzyme), no suppression was detectable.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Hoshino J,Nothacker HP,Schalge S,Kröger Hdoi
10.1016/0014-5793(90)80154-bsubject
Has Abstractpub_date
1990-03-12 00:00:00pages
61-3issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(90)80154-Bjournal_volume
262pub_type
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