Abstract:
:DNA chip technology was used in an attempt to identify target genes responsible for apoptosis induced by etoposide, a p53 activating topoisomerase II inhibitor used clinically as an antitumor agent. 62 Individual mRNAs whose mass changed significantly were identified after screening oligonucleotide arrays capable of detecting 6591 unique human mRNA species. 12 (Nine induced and three repressed) of the etoposide-responsive genes were further studied by Northern analysis and an agreement rate of 92%, was reached. Among the 12 genes studied, two (WAF1/p21 and PCNA) are known p53 regulatory genes, two (glutathione peroxidase and S100A2 calcium-binding protein) appear to be the novel p53 target genes and the others appear to be p53-independent. Based upon these findings, the signalling pathways that possibly mediate etoposide-induced apoptosis are proposed.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Wang Y,Rea T,Bian J,Gray S,Sun Ydoi
10.1016/s0014-5793(99)00136-2keywords:
subject
Has Abstractpub_date
1999-02-26 00:00:00pages
269-73issue
2-3eissn
0014-5793issn
1873-3468pii
S0014-5793(99)00136-2journal_volume
445pub_type
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