Mitochondrial function, content and ROS production in rat skeletal muscle: effect of high-fat feeding.

abstract：:Moloney murine leukemia virus reverse transcriptase (RT) selectively uses deoxyribonucleotides over ribonucleotides (rNTPs) as substrates. Substitution of F155 with valine (F155V) was previously found to increase the enzyme's affinity for rNTPs, though without affecting the V(max) for catalysis, and thereby conferred ...

journal_title：FEBS letters

authors： Liu S,Goff SP,Gao G

更新日期：2006-02-20 00:00:00

abstract：:The mitochondrial dynamin-like GTPase Mgm1 exists as a long (l-Mgm1) and a short isoform (s-Mgm1). They both are essential for mitochondrial fusion. Here we show that the isoforms interact in a homotypic and heterotypic manner. Their submitochondrial distribution between inner boundary membrane and cristae was markedl...

journal_title：FEBS letters

authors： Zick M,Duvezin-Caubet S,Schäfer A,Vogel F,Neupert W,Reichert AS

更新日期：2009-07-07 00:00:00

abstract：:Because phosphorylation of protein kinase C (PKC) may provide a mechanism for regulation of this enzyme, we have examined the ability of two other kinases to phosphorylate PKC. Our results show that casein kinase 1 (CK-1), but not casein kinase 2 (CK-2), can phosphorylate PKC in the absence of Ca2+ and phospholipids. ...

journal_title：FEBS letters

authors： Vila J,Walker JM,Itarte E,Weber MJ,Sando JJ

更新日期：1989-09-11 00:00:00

abstract：:α-Glucosidase hydrolyzes α-glucosides and transfers α-glucosyl residues to an acceptor through transglucosylation. In this study, GH13_31 α-glucosidase BspAG13_31A with high transglucosylation activity is reported in Bacillus sp. AHU2216 and biochemically and structurally characterized. This enzyme is specific to α-(1...

journal_title：FEBS letters

authors： Auiewiriyanukul W,Saburi W,Kato K,Yao M,Mori H

更新日期：2018-07-01 00:00:00

abstract：:We have recorded (13)C nuclear magnetic resonance (NMR) spectra of [3-(13)C]Ala, [1-(13)C]Val-labeled pharaonis phoborhodopsin (ppR or sensory rhodopsin II) incorporated into egg PC (phosphatidylcholine) bilayer, by means of site-directed high-resolution solid-state NMR techniques. Seven (13)C NMR signals from transme...

journal_title：FEBS letters

authors： Arakawa T,Shimono K,Yamaguchi S,Tuzi S,Sudo Y,Kamo N,Saitô H

更新日期：2003-02-11 00:00:00

abstract：:Two major embryonic myosin heavy chains are expressed in embryonic chick breast muscle until the first week after hatching. Of these, one is already detected in the 8-day-old embryo. The other appears on day 12. Two putative slow embryonic isoforms represent minor components transiently expressed between days 8 and 12...

journal_title：FEBS letters

authors： Hofmann S,Düsterhöft S,Pette D

更新日期：1988-10-10 00:00:00

abstract：:The manganese containing center of the oxygen evolving complex accumulates four oxidizing equivalents in the four stepped water oxidation cycle. Based on experiments on electrochromic absorption transients and the reduction rate of the primary electron donor, P680, it has been speculated that the oscillations of these...

journal_title：FEBS letters

authors： Haumann M,Bögershausen O,Junge W

更新日期：1994-11-21 00:00:00

abstract：:In higher eukaryotes the hypermodified guanine analogue, queuine (7-(5-[(1S,4S,5R)-4,5-dihydroxy-2-cyclopentene-1-yl) amino)-methyl)-7-deazaguanine), occurs free or as modified nucleoside (Q) in the anticodon of specific tRNAs. Fast proliferating tissues and tumors contain considerable amounts of free queuine and Q-de...

journal_title：FEBS letters

authors： Langgut W,Kersten H

更新日期：1990-06-04 00:00:00

abstract：:We have examined the activity of protein disulfide isomerase (PDI) and glutaredoxin (Grx) 1, 2 and 3 from Escherichia coli to catalyze the cleavage of glutathionylated ribonuclease A (RNase-SG) by 1 mM GSH to yield reduced RNase. Apparent Km values for RNase-SG were similar, 2-10 microM, for Grx 1, 3 and PDI but Grx I...

journal_title：FEBS letters

authors： Lundström-Ljung J,Vlamis-Gardikas A,Aslund F,Holmgren A

更新日期：1999-01-25 00:00:00

abstract：:L- and D-specific nicotinamide adenine dinucleotide (NAD)-dependent dehydrogenases map to the same structural protein superfamily as defined by the Structural Classification of Proteins (SCOP) and are based on the Rossmann fold type domains. A detailed classification of these domains is proposed using a novel diagnost...

journal_title：FEBS letters

authors： Kutzenko AS,Lamzin VS,Popov VO

更新日期：1998-02-13 00:00:00

abstract：:The yeast PCK1 gene coding for phosphoenolpyruvate carboxykinase (PEPCK) was isolated by functional complementation of pck1 strains from S. cerevisiae. Only one copy of the gene was found per haploid yeast genome. An RNA of about 2 kb which hybridized with a DNA probe internal to the PCK1 gene was found only in cells ...

journal_title：FEBS letters

authors： Valdés-Hevia MD,de la Guerra R,Gancedo C

更新日期：1989-12-04 00:00:00

abstract：:Treatment of human amniotic cells (UAC) with human interferon-alpha (Hu-IFN alpha) or phorbol myristate acetate (PMA) resulted in translocation of protein kinase C (PK-C) activity from the cytosol fraction to that of the membranes. Analysis of 32P incorporation into phospholipid fractions and studies of alterations in...

journal_title：FEBS letters

authors： Premecz G,Markovits A,Bagi G,Farkas T,Földes I

更新日期：1989-06-05 00:00:00

abstract：:The G-protein regulatory (GPR) motif is a approximately 25 amino acid sequence that stabilizes the GDP-bound conformation of Gialpha. To identify additional GPR motifs, we expanded a motif-based search strategy and identified an additional 4 mammalian proteins (WAVE1-3, rat GHRH) and 10 plant proteins with candidate G...

journal_title：FEBS letters

authors： Song KS,Peterson YK,Freidman A,Blumer JB,Sato M,Lanier SM

更新日期：2006-04-03 00:00:00

abstract：:The kinetics of the tRNA cycle is in itself capable of keeping the translational error level almost unaffected by amino acid starvation. There is no need to assume any yet unknown mechanism or property. Kinetic analysis shows that the concentration of aminoacyl-tRNA can stay high even for large reductions in aminoacyl...

journal_title：FEBS letters

authors： Liljenström H

更新日期：1987-10-19 00:00:00

abstract：:In presynaptic nerve terminals, synaptic vesicles are recycled locally via an evolutionarily conserved process that ensures maintenance of neurotransmission as well as structural integrity of synapses. Temperature is a key environmental factor that impacts critical steps involved in fusion, endocytosis and transport i...

journal_title：FEBS letters

authors： Chanaday NL,Kavalali ET

更新日期：2018-11-01 00:00:00

abstract：:The accumulation of pre-PhoS under conditions of PhoS overproduction has been previously described. It is now demonstrated that during the induction of PhoS, a delay in the completion of polypeptide chain elongation can be detected. This delay is related to the extent of jamming of export sites by pre-PhoS or by other...

journal_title：FEBS letters

authors： Anba J,Lazdunski C,Pages JM

更新日期：1986-02-03 00:00:00

abstract：:The effect of Rhodococcus fascians, the causal agent of leafy gall disease, on the mitotic behavior of synchronized tobacco Bright Yellow-2 (BY-2) cells was investigated. Incubation of aphidicolin-synchronized BY-2 cells with R. fascians cells specifically resulted in a broader mitotic index peak, an effect that was l...

journal_title：FEBS letters

authors： Temmerman W,Ritsema T,Simón-Mateo C,Van Montagu M,Mironov V,Inzé D,Goethals K,Holsters M

更新日期：2001-03-09 00:00:00

abstract：:Anomalously high values of photoinduced absorption changes were revealed in the antenna of photosynthetic purple bacteria. They were found to be 4-16 times greater at the bleaching peak of the antenna than at the bleaching peak of the BChl dimer of the reaction center. This is direct proof of excitation delocalization...

journal_title：FEBS letters

authors： Novoderezhkin VI,Razjivin AP

更新日期：1995-07-17 00:00:00

abstract：:The cloning and analysis of a cDNA clone encoding the soybean metalloproteinase obtained by polymerase chain reaction (PCR) and the rapid amplification of cDNA ends (RACE) reaction are described. The cDNA was constructed from poly(A)+ RNA isolated from 15-17 day old leaves. The deduced amino acid sequence of the cDNA ...

journal_title：FEBS letters

authors： Pak JH,Liu CY,Huangpu J,Graham JS

更新日期：1997-03-10 00:00:00

abstract：:We show that anthocyanins can function as potent inhibitors of the formation of nitrated tyrosine in vitro, and clarified how pelargonidin (Pel), which has a mono-hydroxyl group on the B-ring, can scavenge peroxynitrite (ONOO(-)) by detection of the reaction products. Pel was reacted with ONOO(-), then the reaction mi...

journal_title：FEBS letters

authors： Tsuda T,Kato Y,Osawa T

更新日期：2000-11-10 00:00:00

abstract：:In photosynthetic reaction centers, the excitation with light leads to the formation of a charge separated state across the photosynthetic membrane. For the reaction center of purple non-sulphur bacteria, it was previously generally assumed that this primary charge separation could only start with the excitation of th...

journal_title：FEBS letters

authors： van Brederode ME,van Grondelle R

更新日期：1999-07-16 00:00:00

abstract：:Cyanomorpholinoadriamycin (1 microM) was reacted in a transcription buffer with DNA of an initiated transcription complex. Subsequent elongation of the initiated complex revealed permanent transcriptional blockages at 16 sites after only 5 min of drug-DNA reaction time. The most dominant sites were immediately prior t...

journal_title：FEBS letters

authors： Cullinane C,Phillips DR

更新日期：1991-11-18 00:00:00

abstract：:Suppressors of cytokine signaling (SOCS) proteins possess common structures, a SOCS box at the C-terminus and a SH2 domain at their center. These suppressors are inducible in response to cytokines and act as negative regulators of cytokine signaling. The ASB proteins also contain the SOCS box and the ankyrin repeat se...

journal_title：FEBS letters

authors： Kohroki J,Fujita S,Itoh N,Yamada Y,Imai H,Yumoto N,Nakanishi T,Tanaka K

更新日期：2001-09-14 00:00:00

abstract：:Connexins are transmembrane proteins that form gap junction- and hemi-channels. Once inserted into the membrane, hemi-channels (connexons) allow for diffusion of ions and small molecules (<1 kDa) between the extracellular space and the cytosol. Gap junction channels allow diffusion of similar molecules between the cyt...

journal_title：FEBS letters

authors： Meens MJ,Sabine A,Petrova TV,Kwak BR

更新日期：2014-04-17 00:00:00

abstract：:In this study we identify the molecules involved in the MAPK signal transduction pathway (Ras, Raf-1, Mek, Mek-P and MAPK) in highly purified endosomal fractions isolated from rat liver. Biochemical analysis shows that only the early-sorting endocytic compartment contains activated Raf-1 and Mek. Finally, the exogenou...

journal_title：FEBS letters

authors： Pol A,Calvo M,Enrich C

更新日期：1998-12-11 00:00:00

abstract：:There are two common forms of NRH-quinone oxidoreductase 2 (NQO2) in the human population resulting from SNP rs1143684. One has phenylalanine at position 47 (NQO2-F47) and the other leucine (NQO2-L47). Using recombinant proteins, we show that these variants have similar steady state kinetic parameters, although NQO2-L...

journal_title：FEBS letters

authors： Megarity CF,Gill JR,Caraher MC,Stratford IJ,Nolan KA,Timson DJ

更新日期：2014-05-02 00:00:00

abstract：:Botulinum neurotoxins (BoNTs), the causative agent of human botulism, are the most potent naturally occurring toxins known. BoNT/A1, the most studied BoNT, is also used as an important biopharmaceutical. In this study, the biological activity of BoNT/A1 is compared to that of BoNT/A2 using neuronal cell models. The da...

journal_title：FEBS letters

authors： Pier CL,Chen C,Tepp WH,Lin G,Janda KD,Barbieri JT,Pellett S,Johnson EA

更新日期：2011-01-03 00:00:00

abstract：:We have isolated a potent platelet aggregation inducer from the crude venom of Calloselasma rhodostoma (Malayan pit viper), termed rhodoaggretin, with a novel oligomeric structure consisting of a dimer of C-type lectin-like heterodimers. On the basis of its native molecular mass of 66 kDa, and a M(r) of 30 kDa for its...

journal_title：FEBS letters

authors： Wang R,Kong C,Kolatkar P,Chung MC

更新日期：2001-11-23 00:00:00

abstract：:A trisialoganglioside not found before in human tissue was isolated from showed this to be II3(NeuAc)3-LacCer or GT3. The ganglioside constituted 40% of the trisialogangliosides of human lung or 0.2 nmol ganglioside per g tissue. ...

journal_title：FEBS letters

authors： Månsson JE,Mo HQ,Egge H,Svennerholm L

更新日期：1986-02-17 00:00:00

abstract：:Insecticidal proteins or delta-endotoxins of Bacillus thuringiensis are highly toxic to a wide range of agronomically important pests. The toxins are formed of three structural domains. The N-terminal domain is a bundle of eight alpha-helices and is implicated in pore formation in insect midgut epithelial membranes. A...

journal_title：FEBS letters

authors： Chandra A,Ghosh P,Mandaokar AD,Bera AK,Sharma RP,Das S,Kumar PA

更新日期：1999-09-17 00:00:00