Abstract:
:Treatment of human amniotic cells (UAC) with human interferon-alpha (Hu-IFN alpha) or phorbol myristate acetate (PMA) resulted in translocation of protein kinase C (PK-C) activity from the cytosol fraction to that of the membranes. Analysis of 32P incorporation into phospholipid fractions and studies of alterations in fatty acid content for the major phospholipids of IFN-treated cells suggest that phospholipases C and A2 are activated by Hu-IFN alpha. Addition of neomycin (an inhibitor of phospholipase C), as well as mepacrine (an inhibitor of phospholipase A2) to IFN-treated cells inhibited the antiviral activity of Hu-IFN alpha in the vesicular stomatitis virus (VSV)-UAC system used. These observations indicate that (i) activation of PK-C and (ii) diacylglycerol formation, arachidonic acid and/or lysophosphatidylcholine release are important steps in the mechanism of action of IFN.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Premecz G,Markovits A,Bagi G,Farkas T,Földes Idoi
10.1016/0014-5793(89)80635-0subject
Has Abstractpub_date
1989-06-05 00:00:00pages
257-60issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(89)80635-0journal_volume
249pub_type
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