Cloning and sequencing of spinach cDNA clones encoding the 20 kDa PS I polypeptide.

abstract：:The transmembrane protein gp130 is the common signalling receptor subunit for the interleukin-6 (IL-6)-type cytokines. It has recently been shown that the cytoplasmic domain of gp130 contains a dileucine internalization motif and that endocytosis of gp130 occurs signal-independent. Here, we have studied whether gp130 ...

journal_title：FEBS letters

authors： Thiel S,Dahmen H,Martens A,Müller-Newen G,Schaper F,Heinrich PC,Graeve L

更新日期：1998-12-18 00:00:00

abstract：:A Bacillus subtilis strain deficient in seven extracellular proteases was used to produce human mast cell chymase and is a viable expression system for serine proteases and other classes of proteins. Chymase is produced at 0.3-0.5 mg/l and is purified by three chromatography steps. Two crystal forms of PMSF-treated ch...

journal_title：FEBS letters

authors： McGrath ME,Osawa AE,Barnes MG,Clark JM,Mortara KD,Schmidt BF

更新日期：1997-08-25 00:00:00

abstract：:We have constructed cDNA clones covering the entire coding region of mouse, human and rabbit preprocathepsin K mRNA for studies on bone turnover. The clone pMCatK-1 for mouse cathepsin K shares 87% nucleotide homology with the corresponding human and rabbit sequences. Analysis of a panel of mouse tissues for tissue di...

journal_title：FEBS letters

authors： Rantakokko J,Aro HT,Savontaus M,Vuorio E

更新日期：1996-09-16 00:00:00

abstract：:Human Bfl-1 is an anti-apoptotic Bcl-2 family member. Here, we found that Bfl-1 was converted into a potent death-promoting protein by green fluorescent protein (GFP) fusion with its N-terminus. The transient expression of GFP-Bfl-1 induced cytochrome c release and triggered apoptosis in 293T cells, which depended on ...

journal_title：FEBS letters

authors： Ko JK,Choi KH,Kim HJ,Choi HY,Yeo DJ,Park SO,Yang WS,Kim YN,Kim CW

更新日期：2003-09-11 00:00:00

abstract：:The four-step caffeine biosynthetic pathway includes three methylation steps that utilise S-adenosyl-L-methionine (SAM) as the methyl donor. In the process SAM is converted to S-adenosyl-L-homocysteine (SAH) which in turn is hydrolysed to L-homocysteine and adenosine. Significant amounts of radioactivity from [methyl-...

journal_title：FEBS letters

authors： Koshiishi C,Kato A,Yama S,Crozier A,Ashihara H

更新日期：2001-06-15 00:00:00

abstract：:We have set up a model system for familial amyotrophic lateral sclerosis (FALS) by transfecting human neuroblastoma cell line SH-SY5Y with plasmids directing constitutive expression of either wild-type human Cu,Zn superoxide dismutase (Cu,ZnSOD) or a mutant of this enzyme (G93A) associated with FALS. We have tested mi...

journal_title：FEBS letters

authors： Carrì MT,Ferri A,Battistoni A,Famhy L,Gabbianelli R,Poccia F,Rotilio G

更新日期：1997-09-08 00:00:00

abstract：:Ycf1p function is regulated by casein kinase 2α, Cka1p, via phosphorylation of Ser251. Cka1p-mediated phosphorylation of Ycf1p is attenuated in response to high salt stress. Previous results from our lab suggest a role for Ycf1p in cellular resistance to salt stress. Here, we show that Ycf1p plays an important role in...

journal_title：FEBS letters

authors： Paumi CM,Pickin KA,Jarrar R,Herren CK,Cowley ST

更新日期：2012-03-23 00:00:00

abstract：:As yet, the physiological significance of hydroxylation of anandamide and linoleoyl amides is unknown. Therefore, we investigated whether hydroxylation of ODNHEtOH and ODNH2 influences their binding abilities to the CB-1 receptor and whether it alters their reactivity towards a fatty acid amide hydrolase (FAAH) from r...

journal_title：FEBS letters

authors： van der Stelt M,Paoletti AM,Maccarrone M,Nieuwenhuizen WF,Bagetta G,Veldink GA,Finazzi Agrò A,Vliegenthart JF

更新日期：1997-10-06 00:00:00

abstract：:Bovine alpha 2-antiplasmin (alpha 2AP) has been purified and partially characterized. The amino acid composition is very similar to that of human alpha 2AP, and the N-terminal (23 residues determined) and reactive site loop sequences (42 residues determined) are highly homologous to those of the human protein. Compare...

journal_title：FEBS letters

authors： Christensen S,Sottrup-Jensen L

更新日期：1992-11-02 00:00:00

abstract：:To elucidate the subunit composition of axonemal inner-arm dynein, we examined a 38 kDa protein (p38) co-purified with a Chlamydomonas inner arm subspecies, dynein d. We found it is a novel protein conserved among a variety of organisms with motile cilia and flagella. Immunoprecipitation using specific antibody verifi...

journal_title：FEBS letters

authors： Yamamoto R,Yanagisawa HA,Yagi T,Kamiya R

更新日期：2006-11-27 00:00:00

abstract：:Pyrroloquinoline quinone (PQQ) has been claimed to be a component of glutamate decarboxylase from Escherichia coli on the basis of a frequently used procedure in which the protein is extracted with hexanol. We demonstrate that if pyridoxal phosphate (PLP) is not added during the preparation, the apoenzyme prepared fro...

journal_title：FEBS letters

authors： De Biase D,Maras B,John RA

更新日期：1991-01-14 00:00:00

abstract：:Tropomodulin, the P-end (slow-growing end)-capping protein of the actin-tropomyosin filament, and its fragment (C20) of the C-terminal half were studied by synchrotron small-angle X-ray scattering, restoring low-resolution shapes using an ab initio shape-determining procedure. Tropomodulin is elongated (115 A long) an...

journal_title：FEBS letters

authors： Fujisawa T,Kostyukova A,Maéda Y

更新日期：2001-06-01 00:00:00

abstract：:The present study discusses the efficacy of Aloe emodin-8-O-glycoside (AEG), a plant derived anthroquinone, on alleviating insulin resistance and augmenting glycogen synthesis in L6 myotubes and 3T3L1 adipocytes. Dose-dependent increase in glucose uptake activity (GUA) was observed in both cell lines. Immunoblot analy...

journal_title：FEBS letters

authors： Anand S,Muthusamy VS,Sujatha S,Sangeetha KN,Bharathi Raja R,Sudhagar S,Poornima Devi N,Lakshmi BS

更新日期：2010-07-16 00:00:00

abstract：:Trace amounts of gangliosides were found in rabbit skeletal muscle sarcoplasmic reticulum and their main part was shown, by high performance thin layer chromatography. to be GM3. Addition of GM3 to the soybean phospholipids used for reconstitution of proteoliposomes markedly increased ATP hydrolysis as well as Ca2+ up...

journal_title：FEBS letters

authors： Wang LH,Tu YP,Yang XY,Tsui ZC,Yang FY

更新日期：1996-06-17 00:00:00

abstract：:The Ca(2+)-calmodulin dependent protein phosphatase, calcineurin, is thought to mediate the action of the two immunosuppressants, cyclosporin A (CsA) and FK506. Calcineurin from all species consists of a catalytic A subunit and a regulatory peptide B, which plays an essential role in catalysis. The enzymatic function ...

journal_title：FEBS letters

authors： Chaudhuri B,Hämmerle M,Fürst P

更新日期：1995-01-03 00:00:00

abstract：:Azide-binding to the heme-copper binuclear center of bo-type ubiquinol oxidase from Escherichia coli was investigated with Fourier-transform infrared spectroscopy. Deconvolution analyses of infrared spectra of the azide (14N3)-inhibited air-oxidized form showed a major infrared azide antisymmetric stretching band at 2...

journal_title：FEBS letters

authors： Tsubaki M,Mogi T,Hori H

更新日期：1999-04-23 00:00:00

abstract：:ATM (ataxia telangiectasia mutated), the gene mutated in ataxia telangiectasia, is related to a family of large phosphatidylinositol 3-kinase domain-containing proteins involved in cell cycle control and DNA repair. We found that ATM(-/-) DT40 cells were more susceptible than wild-type cells to apoptosis induced not o...

journal_title：FEBS letters

authors： Takao N,Li Y,Yamamoto K

更新日期：2000-04-21 00:00:00

abstract：:Rat liver fructose 1,6-bisphosphatase can be protected against partial inactivation by N-ethylmaleimide by low concentrations of fructose 2,6-bisphosphate or high concentrations of fructose 1,6-bisphosphate. The partially inactivated enzyme has a much reduced sensitivity to high substrate inhibition and has lost the s...

journal_title：FEBS letters

authors： Meek DW,Nimmo HG

更新日期：1983-08-22 00:00:00

abstract：:Natural freeze tolerance depends on cellular adaptations that address the multiple stresses imposed on cells during freezing. These adaptations preserve viability by suppressing energy-expensive cell processes in the frozen state. In this study, we explore the freeze-responsive expression of microRNA in hatchling pain...

journal_title：FEBS letters

authors： Biggar KK,Storey KB

更新日期：2015-11-30 00:00:00

abstract：:Prostaglandin E2 receptors (EPR), which belong to the family of heterotrimeric G protein-coupled ectoreceptors with seven transmembrane domains, can be classified into four subtypes according to their ligand binding and G protein coupling specificity. Of these, EP3betaR is coupled to Gi, whereas EP4R is coupled to Gs....

journal_title：FEBS letters

authors： Neuschäfer-Rube F,Hänecke K,Püschel GP

更新日期：1997-09-29 00:00:00

abstract：:Plant G proteins of the ROP/RAC family regulate cellular processes including cytoskeletal rearrangement in polar growth. Activation of the ROP molecular switch is triggered by guanine nucleotide exchange factors. Plant-specific RopGEFs are exclusively active on ROPs despite their high homology to animal Rho proteins. ...

journal_title：FEBS letters

authors： Fricke I,Berken A

更新日期：2009-01-05 00:00:00

abstract：:We have recently demonstrated that myocardial adaptation to ischemia triggers a tyrosine kinase regulated signaling pathway leading to the translocation and activation of p38 MAP kinase and MAPKAP kinase 2. Since oxidative stress is developed during ischemic adaptation and since free radicals have recently been shown ...

journal_title：FEBS letters

authors： Maulik N,Sato M,Price BD,Das DK

更新日期：1998-06-16 00:00:00

abstract：:The insulin-like growth factor binding proteins (IGFBPs) are responsible for regulation of the effects and the bioavailability of the insulin-like growth factors (IGFs). We screened for circulating fragments of human IGFBP-5 in human hemofiltrate. Identification of IGFBP-5 peptides in the fractions of our peptide bank...

journal_title：FEBS letters

authors： Ständker L,Wobst P,Mark S,Forssmann WG

更新日期：1998-12-18 00:00:00

abstract：:Frame shift mutations of the polyglutamine binding protein-1 (PQBP1) gene lead to total or partial truncation of the C-terminal domain (CTD) and cause mental retardation in human patients. Interestingly, normal Drosophila homologue of PQBP-1 lacks CTD. As a model to analyze the molecular network of PQBP-1 affecting in...

journal_title：FEBS letters

authors： Yoshimura N,Horiuchi D,Shibata M,Saitoe M,Qi ML,Okazawa H

更新日期：2006-04-17 00:00:00

abstract：:The products of the isiAB operon are a chlorophyll antenna protein (IsiA) and flavodoxin (IsiB), which accumulate in cyanobacteria grown under iron starvation conditions. Here we show that strong light triggers de-repression of isiAB transcription and leads to IsiA and flavodoxin accumulation under iron replete condit...

journal_title：FEBS letters

authors： Havaux M,Guedeney G,Hagemann M,Yeremenko N,Matthijs HC,Jeanjean R

更新日期：2005-04-25 00:00:00

abstract：:The N-terminus of MDM2 proto-oncoprotein interacts with p53 and down modulates p53 activity by inhibiting transcriptional activity and promoting p53 degradation. MDMX is structurally related to MDM2 and also binds to p53. However, the function of MDMX has not been clarified yet. We found that MDM2 hetero-oligomerized ...

journal_title：FEBS letters

authors： Tanimura S,Ohtsuka S,Mitsui K,Shirouzu K,Yoshimura A,Ohtsubo M

更新日期：1999-03-19 00:00:00

abstract：:A variant form of the heptacosapeptide amide secretin, with C-terminal -Val-Gly-Lys-Arg instead of valine amide, has been isolated from porcine upper intestinal tissue. Unexpectedly, this triacontapeptide exhibited a substantially higher bioactivity than the heptacosapeptide amide. ...

journal_title：FEBS letters

authors： Gafvelin G,Carlquist M,Mutt V

更新日期：1985-05-20 00:00:00

abstract：:The MAP kinase module (Raf/MAPKKK-MAPKK-MAPK) has been shown to be sequentially activated after mitogenic stimulation. Here we demonstrate, by site directed mutagenesis, that MAPK is able to retrophosphorylate its own activator, MAPKK, on two threonine residues Thr-292 and Thr-386 in vitro, and that these sites are al...

journal_title：FEBS letters

authors： Brunet A,Pagès G,Pouysségur J

更新日期：1994-06-13 00:00:00

abstract：:Biological functions of transcribed spacer regions in eucaryotic pre-rRNAs remain elusive so far. Utilization of the comparative approach to analyse their secondary structure has been impeded by the extensive sequence divergence observed among most of the specimens available to date. However, we have taken advantage o...

journal_title：FEBS letters

authors： Michot B,Despres L,Bonhomme F,Bachellerie JP

更新日期：1993-02-01 00:00:00

abstract：:We have previously demonstrated that the taurine uptake and transporter (TAUT) mRNA expression were upregulated by tumor necrosis factor alpha (TNF-alpha) in Caco-2 cells. In this present study, the signaling molecules related to this upregulation were investigated. Pyrrolidine dithiocarbamate, a nuclear factor kappaB...

journal_title：FEBS letters

authors： Mochizuki T,Satsu H,Shimizu M

更新日期：2005-06-06 00:00:00