Low-temperature microRNA expression in the painted turtle, Chrysemys picta during freezing stress.

abstract：:Temperature-jump measurements were carried out on myosin subfragment 1 (S1) labeled at Cys-707 with 5-(iodoacetamido)fluorescein (S1-AF). The relaxation was monitored by following the increase in the fluorescence intensity of the attached probe after a jump of 5.8 degrees C. A single relaxation process was observed ov...

journal_title：FEBS letters

authors： Lin SH,Cheung HC

更新日期：1992-06-15 00:00:00

abstract：:The black-eyed pea trypsin and chymotrypsin inhibitor (BTCI) is a member of the Bowman-Birk protease inhibitor (BBI) family. The three-dimensional model of the BTCI-chymotrypsin complex was built based on the homology to Bowman-Birk inhibitors with known structures. An extensive theoretical and experimental study of t...

journal_title：FEBS letters

authors： de Freitas SM,de Mello LV,da Silva MC,Vriend G,Neshich G,Ventura MM

更新日期：1997-06-09 00:00:00

abstract：:The PDE4 catalytic machinery comprises, in part, two divalent cations in a binuclear motif. Here we report that PDE4A4 expressed in Sf9 cells exhibits a biphasic Mg(2+) dose-response (EC(50) of 0.15 and >10 mM) in catalyzing cAMP hydrolysis. In vitro phosphorylation of PDE4A4 by the PKA-catalytic subunit increases the...

journal_title：FEBS letters

authors： Laliberté F,Liu S,Gorseth E,Bobechko B,Bartlett A,Lario P,Gresser MJ,Huang Z

更新日期：2002-02-13 00:00:00

abstract：:Decades of neuroscience research have shed light on the hippocampus as a key structure for the formation of episodic memory. The hippocampus is divided into distinct subfields - CA1, CA2 and CA3. While accumulating evidence points to cellular and synaptic heterogeneity within each subfield, this heterogeneity has not ...

journal_title：FEBS letters

authors： Igarashi KM,Ito HT,Moser EI,Moser MB

更新日期：2014-08-01 00:00:00

abstract：:Anthrax is a severe bacterial infection that occurs when Bacillus anthracis spores gain access into the body and germinate in macrophages, causing septicemia and toxemia. Anthrax toxin is a binary A-B toxin composed of protective antigen (PA), lethal factor (LF), and edema factor (EF). PA mediates the entry of either ...

journal_title：FEBS letters

authors： Ascenzi P,Visca P,Ippolito G,Spallarossa A,Bolognesi M,Montecucco C

更新日期：2002-11-20 00:00:00

abstract：:The sequence of seminalplasmin, a basic antimicrobial and transcription-inhibitory protein from bovine seminal plasma, has been determined using an automated sequenator. This sequence is slightly different from that reported earlier by Theil and Scheit [(1983) EMBO J. 2, 1159-1163] and identical with that of caltrin, ...

journal_title：FEBS letters

authors： Sitaram N,Kumari VK,Bhargava PM

更新日期：1986-06-09 00:00:00

abstract：:A mathematical model was developed to estimate flux control coefficients (Co) from titration studies with specific non-competitive inhibitors. In contrast to the normally used graphical determination the model pays regard to the dissociation equilibrium (KD) that exists between inhibitor and its binding sites (Eo) as ...

journal_title：FEBS letters

authors： Gellerich FN,Kunz WS,Bohnensack R

更新日期：1990-11-12 00:00:00

abstract：:All eukaryotic cells enclose their genome within a dedicated, membrane-bound organelle termed the nucleus, which functions to partition gene transcription from sites of protein translation in the cytoplasm. Despite a great deal of research effort, basic questions about chromosome structure and gene expression mechanis...

journal_title：FEBS letters

authors： Trinkle-Mulcahy L,Lamond AI

更新日期：2008-06-18 00:00:00

abstract：:The amyloid precursor protein (APP) is proteolytically processed by β- and γ-secretases to release amyloid-β peptide (Aβ), the main component found in senile plaques of Alzheimer's disease (AD) patient brains. Alternatively, APP can be cleaved within the Aβ sequence by α-secretase, thus precluding the generation of Aβ...

journal_title：FEBS letters

authors： León-Otegui M,Gómez-Villafuertes R,Díaz-Hernández JI,Díaz-Hernández M,Miras-Portugal MT,Gualix J

更新日期：2011-07-21 00:00:00

abstract：:In mammalian cells, the Bcl-2 and Bcl-x(L) proteins suppress programmed cell death whereas the topographically similar Bax protein accelerates the apoptotic process. Recently published data suggest that expression of the human Bax-alpha gene is lethal for the yeast Saccharomyces cerevisiae and that this toxicity can b...

journal_title：FEBS letters

authors： Greenhalf W,Stephan C,Chaudhuri B

更新日期：1996-02-12 00:00:00

abstract：:We analysed the biochemical properties of insulin receptors of a Type A insulin resistant patient with a single heterozygous point mutation substituting Gln for Arg1174. Insulin binding capacity and affinity to Epstein-Barr virus transformed lymphocytes was normal. Quantitative analysis of autophosphorylation and subs...

journal_title：FEBS letters

authors： Moritz W,Froesch ER,Böni-Schnetzler M

更新日期：1994-09-05 00:00:00

abstract：:Amyloid precursor protein (APP) gene expression was investigated in primary cultures of neurons, astrocytes, microglial cells and oligodendrocytes. Neurons from various rat brain regions, as well as oligodendrocytes, contained RNA encoding APP695, while astrocytes and microglial cells expressed high levels of RNAs for...

journal_title：FEBS letters

authors： Gaul G,Dutly F,Frei K,Foguet M,Lübbert H

更新日期：1992-08-03 00:00:00

abstract：:A lowered efficiency of oxidative phosphorylation was recently found in a Leber hereditary optic neuropathy (LHON) proband carrying a mutation in the mtDNA gene for subunit 6 of the membrane-bound F0 segment of the F1F0-ATP synthase [9]. This phenotype was transferred to cytoplasmic hybrid cells together with the muta...

journal_title：FEBS letters

authors： Majander A,Lamminen T,Juvonen V,Aula P,Nikoskelainen E,Savontaus ML,Wikström M

更新日期：1997-07-28 00:00:00

abstract：:The phosphopeptide Ser (P)-Ser(P)-Ser-(P)-Glu-Glu-Ser22-Ile-Thr, reproducing the 17-24 segment of beta-casein A2 including the seryl residue (Ser-22) which is targeted by casein kinase-1 was synthesized and used as model substrate for this enzyme. Its phosphorylation efficiency is actually higher than that of intact b...

journal_title：FEBS letters

authors： Meggio F,Perich JW,Reynolds EC,Pinna LA

更新日期：1991-06-03 00:00:00

abstract：:The effective synthesis of the envelope antigen F1 of Y. pestis in E. coli HB101 is mediated by the expression of the caf1M gene. This gene was sequenced, and the protein encoded was found to have a significant homology with the chaperone protein PapD of uropathogenic E. coli. The data presented allow one to suppose C...

journal_title：FEBS letters

authors： Galyov EE,Karlishev AV,Chernovskaya TV,Dolgikh DA,Smirnov OYu,Volkovoy KI,Abramov VM,Zav'yalov VP

更新日期：1991-07-29 00:00:00

abstract：:The gene for titin, a 4MDa myofibrillar protein, was analysed in golden hamster DNAs from different sources, using human cDNA probes and PCR. In the DNA from the BHK-21-Bi subline of baby hamster kidney cells, extended sequences coding for Z-line associated domains were missing, indicating a deletion that renders titi...

journal_title：FEBS letters

authors： Jäckel M,Witt C,Antonova O,Curdt I,Labeit S,Jockusch H

更新日期：1997-05-12 00:00:00

abstract：:The neurogenic gene Drosophila big brain (bib) has a high sequence homology to aquaporin-4. However, its cellular functions in Drosophila neurogenesis have remained elusive. Here we investigated cell adhesion, and the ion and water permeability of Bib. The adhesive function was examined by a cell aggregation assay usi...

journal_title：FEBS letters

authors： Tatsumi K,Tsuji S,Miwa H,Morisaku T,Nuriya M,Orihara M,Kaneko K,Okano H,Yasui M

更新日期：2009-06-18 00:00:00

abstract：:The receptor structure of Clostridium botulinum neurotoxin type A was analysed by TLC immunostaining. GQ1b was found to be the most potent receptor, and the neurotoxin also bound to GT1b and GD1a, but not to GM3, GM2, GM1, GD3, GD1b and GT1a. Optimum binding of neurotoxin to the ganglioside appeared in 0.01 M phosphat...

journal_title：FEBS letters

authors： Takamizawa K,Iwamori M,Kozaki S,Sakaguchi G,Tanaka R,Takayama H,Nagai Y

更新日期：1986-06-09 00:00:00

abstract：:alpha-Dystroglycan was quantitatively enriched from mammalian brain based on its uniform reactivity with Vicia villosa agglutinin and resolved into sub-populations possessing or lacking the sulfated glucuronic acid epitope recognized by monoclonal antibody HNK-1. We generated a new monoclonal antibody specific for a g...

journal_title：FEBS letters

authors： McDearmon EL,Combs AC,Sekiguchi K,Fujiwara H,Ervasti JM

更新日期：2006-06-12 00:00:00

abstract：:Many genes are regulated by the intracellular calcium, protein kinase C (PKC) and protein kinase A (PKA) pathways and it has been shown that these pathways synergize in some cell types, whereas they antagonize in others. Here we show that the calcium and PKC pathways suppress the effects mediated by the PKA pathway in...

journal_title：FEBS letters

authors： Döbbeling U,Berchtold MW

更新日期：1996-08-05 00:00:00

abstract：:Although neurokinin A (NKA), a tachykinin peptide with sequence homology to substance P (SP), is a weak competitor of radiolabeled SP binding to the NK-1 receptor (NK-1R), more recent direct binding studies using radiolabeled NKA have demonstrated an unexpected high-affinity interaction with this receptor. To document...

journal_title：FEBS letters

authors： Bremer AA,Leeman SE,Boyd ND

更新日期：2000-12-01 00:00:00

abstract：:In the first committed reaction of chlorophyll biosynthesis, magnesium chelatase couples ATP hydrolysis to the thermodynamically unfavorable Mg(2+) insertion into protoporphyrin IX (ΔG°' of circa 25-33 kJ·mol(-1) ). We explored the thermodynamic constraints on magnesium chelatase and demonstrate the effect of nucleoti...

journal_title：FEBS letters

authors： Adams NB,Brindley AA,Hunter CN,Reid JD

更新日期：2016-06-01 00:00:00

abstract：:USP4, 11 and 15 are three closely related paralogues of the ubiquitin specific protease (USP) family of deubiquitinating enzymes. The DUSP domain and the UBL domain in these proteins are juxtaposed which may provide a functional unit conferring specificity. We determined the structures of the USP15 DUSP-UBL double dom...

journal_title：FEBS letters

authors： Elliott PR,Liu H,Pastok MW,Grossmann GJ,Rigden DJ,Clague MJ,Urbé S,Barsukov IL

更新日期：2011-11-04 00:00:00

abstract：:The influence of the esterification of the 3'(2') hydroxyl group of tRNA on the hydrolysis by venom phosphodiesterase (Crotalus adamanteus) was studied. It was shown that this esterification does not change the rate of hydrolysis. At pH 7-8, no significant differences were observed either when the alpha amino group of...

journal_title：FEBS letters

authors： Yot P,Gueguen P,Chapeville F

更新日期：1968-08-01 00:00:00

abstract：:The tuf gene of eubacteria, encoding the EF-tu elongation factor, was duplicated early in the evolution of the taxon. Phylogenetic and genomic location analysis of 20 complete eubacterial genomes suggests that this ancient duplication has been differentially lost and maintained in eubacteria. ...

journal_title：FEBS letters

authors： Lathe WC 3rd,Bork P

更新日期：2001-08-03 00:00:00

abstract：:Sequence comparisons of 5 S and other ribosomal RNAs by segments can be useful in understanding anomalous primary and secondary structures and in assessing phylogenetic relationships. In a segmented analysis, the 5'-half of the Chlamydomonas reinhardii chloroplast 5 S ribosomal RNA is found to have a very close sequen...

journal_title：FEBS letters

authors： Delihas N

更新日期：1987-09-14 00:00:00

abstract：:CHO SSF3 cells grow as a suspension culture in unmodified commercial medium with only low-molecular weight ingredients. Continuous serum-free culture unexpectedly induced expression of a low dihydrofolate reductase activity in the originally dhfr- CHO cells. Nevertheless, it was possible with methotrexate to induce am...

journal_title：FEBS letters

authors： Gandor C,Leist C,Fiechter A,Asselbergs FA

更新日期：1995-12-27 00:00:00

abstract：:For good function, membrane lipids have to be arranged appropriately and be in the correct physical state. In poikilotherms, exposure to cold stress or heat shock can alter membrane properties such that, unless they are corrected quickly, damage and, possibly, death can result. Low temperature stress is countered by m...

journal_title：FEBS letters

authors： Guschina IA,Harwood JL

更新日期：2006-10-09 00:00:00

abstract：:Autophagy is a bulk degradation process conserved among eukaryotes. In macro-autophagy, autophagosomes sequester cytoplasmic components and deliver their contents to lysosomes/vacuoles. Autophagosome formation requires the conjugation of Atg8, a ubiquitin-like protein, to phosphatidylethanolamine (PE). Here we report ...

journal_title：FEBS letters

authors： Hanada T,Satomi Y,Takao T,Ohsumi Y

更新日期：2009-04-02 00:00:00

abstract：:X-linked inhibitor of apoptosis protein (XIAP) is a potent antagonist of caspases, and functions as a ubiquitin-E3 ligase by itself and for caspases. Recently, NEDD8, a ubiquitin-like modifier, has been suggested to be used for modification of caspase-7 mediated by XIAP. However, it is not clear whether caspase-7 is a...

journal_title：FEBS letters

authors： Nagano T,Hashimoto T,Nakashima A,Kikkawa U,Kamada S

更新日期：2012-06-04 00:00:00