Abstract:
:The N-terminus of MDM2 proto-oncoprotein interacts with p53 and down modulates p53 activity by inhibiting transcriptional activity and promoting p53 degradation. MDMX is structurally related to MDM2 and also binds to p53. However, the function of MDMX has not been clarified yet. We found that MDM2 hetero-oligomerized with MDMX through their C-terminal RING finger domains. Yeast two-hybrid analysis revealed that the hetero-oligomerization between MDMX and MDM2 was more stable than the homo-oligomerization of each protein. MDM2 has been shown to be degraded by the ubiquitin-proteasome pathway, while MDMX was a stable protein. Interaction of MDMX with MDM2 through the C-terminal RING finger domains resulted in inhibiting degradation of MDM2. These data indicate that MDMX functions as a regulator of MDM2.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Tanimura S,Ohtsuka S,Mitsui K,Shirouzu K,Yoshimura A,Ohtsubo Mdoi
10.1016/s0014-5793(99)00254-9keywords:
subject
Has Abstractpub_date
1999-03-19 00:00:00pages
5-9issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(99)00254-9journal_volume
447pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Structure-based drug design requires the development of efficient computer programs for exploring the structural compatibility of various flexible ligands with a given receptor. While various algorithms are available for finding docked conformations, selecting a target function that can reliably score the conformation...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00276-1
更新日期:1996-04-08 00:00:00
abstract::Prepro-vasoactive intestinal peptide (prepro VIP) was expressed in NIH 3T3 cells, and the prepro VIP-derived peptides produced by the cells were analyzed by chromatography combined with sequence-specific radio-immunoanalysis. In accordance with what has previously been reported on processing in non-endocrine cell line...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)80237-8
更新日期:1994-03-14 00:00:00
abstract::Amino acid sequence alignment of the amino-terminal of thrombomodulin and pancreatic stone protein with the hepatic asialoglycoprotein receptor shows that these proteins are homologous. From the known disulfide bridge pattern of other proteins belonging to the same family two disulfide bonds can be predicted. The homo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80700-2
更新日期:1988-04-11 00:00:00
abstract::The interaction of Bordetella pertussis adenylate cyclase toxin (CyaA) with complement receptor 3 (CR3, CD11b/CD18) involves N-linked oligosaccharide chains. To investigate the relative importance of the individual N-glycans of CR3 for toxin activity, the asparagine residues of the consensus N-glycosylation sites of C...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.12.023
更新日期:2015-01-30 00:00:00
abstract::The pre-steady state process in the thermolysin-catalyzed hydrolysis of Cbz-Gly-Phe-Ala was observed at pH 4.5 by fluorescence stopped-flow method using Dns-Phe as a displacement probe. After the confirmation of the pre-equilibrium hypothesis for the binary interaction, the nonlinear substrate concentration dependence...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00242-2
更新日期:1995-04-03 00:00:00
abstract::A colostral proline-rich polypeptide complex (PRP) consisting of over 30 peptides shows beneficial effects in Alzheimer's disease (AD) patients when administered in the form of sublinqual tablets called Colostrinin. The aim of the present studies was to investigate whether nanopeptide fragment of PRP (NP) - one of the...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.11.053
更新日期:2009-01-05 00:00:00
abstract::Mitochondrial dihydroorotate dehydrogenase (DHOdehase; EC 1.3.99.11) is a target of anti-proliferative, immunosuppressive and anti-parasitic agents. Here, redoxal, (2,2'-[3,3'-dimethoxy[1, 1'-biphenyl]-4,4'-diyl)diimino]bis-benzoic acid, was studied with isolated mitochondria and the purified recombinant human and rat...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01117-0
更新日期:2000-02-04 00:00:00
abstract::Multiple protein kinase C (PKC) theta species, identified in an erythroleukaemia cell line, have been characterised in terms of their molecular properties and intracellular distribution. PKCthetas localised in the detergent-soluble cell fraction have an Mr of 76 kDa (theta-76) and contain Thr538 or pThr538 in the kina...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)01073-1
更新日期:2003-11-06 00:00:00
abstract::A Bacillus subtilis strain deficient in seven extracellular proteases was used to produce human mast cell chymase and is a viable expression system for serine proteases and other classes of proteins. Chymase is produced at 0.3-0.5 mg/l and is purified by three chromatography steps. Two crystal forms of PMSF-treated ch...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00962-9
更新日期:1997-08-25 00:00:00
abstract::Glycosylphosphatidylinositol (GPI) is synthesized in the endoplasmic reticulum (ER) and added onto proteins to form GPI-anchored proteins. Among the many proteins involved in this process, ACAT-related enzyme-2 required for viability 1 (Arv1) is a candidate, functioning as a flippase that translocates GPI intermediate...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13843
更新日期:2020-08-01 00:00:00
abstract::RNA-binding protein kinase from amphibian oocytes modifies serine and threonine residues in the molecules of substrates and utilizes both ATP and GTP. Low concentrations of heparin inhibit protein kinase. The foregoing suggests that this enzyme is casein kinase II. It is shown that RNA-binding proteins lack active for...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)81363-0
更新日期:1984-05-07 00:00:00
abstract::Using a radioimmunoassay for the peptide sequence Arg-Phe-NH2 (RFamide), a novel peptide has been purified from acetic acid extracts of the sea anemone Anthopleura elegantissima. This peptide has the structure less than Glu-Gly-Leu-Arg-Trp-NH2, and was named Antho-RWamide II. Antho-RWamide II is a neuropeptide. Its st...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80560-x
更新日期:1988-10-24 00:00:00
abstract::The actin-depolymerising factor (ADF)/cofilin group of proteins are stimulus-responsive actin-severing proteins, members of which are regulated by reversible phosphorylation. The phosphorylation site on the maize ADF, ZmADF3, is Ser-6 but the kinase responsible is unknown [Smertenko et al., Plant J. 14 (1998) 187-193]...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02528-5
更新日期:2001-06-15 00:00:00
abstract::In the methylerythritol phosphate pathway for isoprenoid biosynthesis, the GcpE/IspG enzyme catalyzes the conversion of 2-C-methyl-d-erythritol 2,4-cyclodiphosphate into (E)-4-hydroxy-3-methylbut-2-enyl diphosphate. This reaction requires a double one-electron transfer involving a [4Fe-4S] cluster. A thylakoid prepara...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.01.082
更新日期:2006-03-06 00:00:00
abstract::We investigated the role of p53 in nuclear factor (NF)-kappaB dependent, platelet-activating factor (PAF)-induced vascular endothelial growth factor (VEGF) expression. Transfected NF-kappaB subunits in ECV304 cells increased the tumor necrosis factor-alpha promoter activity, which was completely inhibited by p53. Tran...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.04.042
更新日期:2006-05-29 00:00:00
abstract::Large conductance voltage-dependent and Ca(2+)-modulated K(+) channels play a crucial role in myometrium contractility. Western blots and immunocytochemistry of rat uterine sections or isolated cells show that MaxiK channel protein signals drastically decrease towards the end of pregnancy. Consistent with a transcript...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01394-0
更新日期:1999-11-05 00:00:00
abstract::BSP-30K is a major acidic glycoprotein of bovine seminal plasma. It displays heparin-, gelatin-, and phospholipid-binding activities. BSP-30K binds to spermatozoa upon ejaculation and is thought to play a role in sperm capacitation. We have determined its amino acid sequence, disulfide bonds, and 0-glycosylation sites...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(96)01310-5
更新日期:1996-12-09 00:00:00
abstract::The farnesoid X receptor (FXR) is a nuclear receptor activated by bile acids (BAs). In response to ligand-binding, FXR regulates many genes involved in BA, lipid, and lipoprotein metabolism. To identify new FXR target genes, microarray technology was used to profile total RNA extracted from HepG2 cells treated with th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.04.026
更新日期:2004-05-21 00:00:00
abstract::C2cd4c, encoded by a gene belonging to the C2cd4 family, contains a C2 domain conserved across species and is localized to the cytoplasm. To examine the role of C2cd4c in the pancreas, we studied its localization and generated C2cd4c knockout (KO) mice. C2cd4c was expressed in pancreatic endocrine progenitors at early...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12271
更新日期:2016-08-01 00:00:00
abstract::The plant hormone cytokinin plays a major role in regulating plant growth and development. Here we generated cytokinin-reduction Arabidopsis plants by overexpressing a heterologous cytokinin oxidase gene DSCKX1 from Dendrobium orchid. These transgenic plants exhibited reduced biomass, rapid root growth, decreased abil...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)01259-6
更新日期:2003-12-04 00:00:00
abstract::Stimulation of human platelets increases cytoplasmic pH (pHi) via activation of Na+/H+ exchange. We have determined the effect of inhibiting Na+/H+ exchange on (i) thrombin-induced Ca2+ mobilization and (ii) turnover of 32P-labelled phospholipids. Blocking Na+/H+ exchange by removal of extracellular Na+ or by ethyliso...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)81569-7
更新日期:1987-02-09 00:00:00
abstract::We employed cDNA representational difference analysis to identify new genes that are upregulated by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in a human hepatoblastoma cell line HepG2. We isolated several TCDD-responsive cDNAs. Sequence analysis revealed that one of them encodes E16/CD98LC/hLAT1, an integral membrane...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01574-4
更新日期:1999-12-03 00:00:00
abstract::alpha2-Macroglobulin was derivatized with a sulfhydryl cross-linker and sandwiched between a mica substrate and a silicon nitride tip, both coated with gold, of an atomic force microscope and force curve measurement was carried out. An extensive downward deflection of the cantilever was observed in the retracting real...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00319-5
更新日期:1996-04-29 00:00:00
abstract::To characterize the proteins involved in cell clump/cell adhesion of insect cellular defense reactions, we induced the cell clump/cell adhesion reaction in vitro with the hemolymph of larvae of the coleopteran insect, Tenebrio molitor. The 72 kDa protein was specifically enriched in the residues of cell clump/cell adh...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00067-8
更新日期:1999-02-12 00:00:00
abstract::This article presents an overview of the simulation studies of the behaviour of multihaem cytochromes using theoretical/computational methodologies, with an emphasis on cytochrome c(3). It starts with the first studies using rigid molecules and continuum electrostatic models, where protonation and redox events were tr...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2011.10.019
更新日期:2012-03-09 00:00:00
abstract::Studies were undertaken to further elucidate the mechanism(s) by which bradykinin-dependent phosphoinositide metabolism takes place in neuroblastoma X glioma hybrid NG108-15 cells [(1984) J. Biol. Chem. 259, 10201-10207] using [3H]inositol-labelled cells. Bradykinin produced net increases in the level of [3H]inositol ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80301-x
更新日期:1985-02-25 00:00:00
abstract::Using antibody against the Rho binding domain of ROKalpha, two neuronal phosphoproteins of 62 and 80 kDa were co-immunoprecipitated from brain extracts. Peptide analysis revealed their identity as collapsin response mediator proteins (CRMPs); p62 was CRMP-2 whereas p80 was a novel splice form of CRMP-1 with an extende...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03736-5
更新日期:2002-12-18 00:00:00
abstract::Reticulocyte-type 15-lipoxygenase is known to dioxygenate phospholipids without preceding action of phospholipases A2 (PLA2). Therefore we studied the reaction of the secretory PLA2s (sPLA2) from pancreas and snake venom, and of the human cytosolic PLA2 (cPLA2) with 1-palmitoyl-2-arachidonoyl phosphatidylcholine (PAPC...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01024-2
更新日期:1998-09-04 00:00:00
abstract::The transmembrane protein MIG-13 is a key regulator required for anterior migration of neural cells in Caenorhabditis elegans, but the signaling mechanisms involved remain unknown. Here, we isolated a suppressor mutation in the unc-71/adm-1 gene, which rescued the AVM neuron migration defect in mig-13 mutants. Genetic...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.01.031
更新日期:2012-03-23 00:00:00
abstract::Exposure of protein kinase C to low concentrations of either N-chlorosuccinimide or H2O2 resulted in rapid and parallel loss of phosphotransferase activity and phorbol ester binding. This oxidative inactivation of protein kinase C also occurred in intact cells exposed to a low concentration of H2O2. With H2O2 treatmen...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)81164-x
更新日期:1987-12-10 00:00:00