当前位置： SCI文献检索 > FEBS LETTERS期刊下所有文献 > Isolation and characterization of circulating 13-kDa C-terminal fragments of human insulin-like growth factor binding protein-5.

Isolation and characterization of circulating 13-kDa C-terminal fragments of human insulin-like growth factor binding protein-5.

Abstract：

:The insulin-like growth factor binding proteins (IGFBPs) are responsible for regulation of the effects and the bioavailability of the insulin-like growth factors (IGFs). We screened for circulating fragments of human IGFBP-5 in human hemofiltrate. Identification of IGFBP-5 peptides in the fractions of our peptide bank generated from hemofiltrate was performed by their immunoreactivity and their capacity to bind IGF-I. Different fragments of IGFBP-5 with molecular sizes from 12 to 25 kDa were identified. C-terminal peptides of IGFBP-5 with molecular masses of 13.3 and 13.5 kDa were purified by consecutive chromatographic steps and sequenced. Sequence analysis of the peptides revealed the (double) sequences (K)FVGGAENXAHPRII and MVPRAVYLPNXDRKG. In addition, a smaller fragment with Mr 2722 of the central IGFBP-5 region was purified and showed the sequence HTRISELKAEAVKKDRRKKLTQS (residues 121-143) indicating plasma proteolysis of IGFBP-5 C-terminal to amino acids Lys-120, Ser-143, Lys-144, and Arg-188. According to mass spectrometric and sequence analysis, Thr-152 was shown to be O-glycosylated. Fractions containing C-terminal IGFBP-5 fragments revealed significant IGF-I binding properties. Our results indicate that plasma proteolysis of IGFBP-5 preferentially occurs C-terminally to basic residues and generates different C-terminal fragments, possibly acting in an IGF-dependent manner and bearing intrinsic biological functions.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Ständker L,Wobst P,Mark S,Forssmann WG

doi

10.1016/s0014-5793(98)01497-5

subject

Has Abstract

pub_date

1998-12-18 00:00:00

pages

281-6

issue

2

eissn

0014-5793

issn

1873-3468

pii

S0014-5793(98)01497-5

journal_volume

441

pub_type

杂志文章

相关文献

FEBS LETTERS文献大全
  • Identification of nuclear localization signals within the zinc fingers of the WT1 tumor suppressor gene product.

    abstract：:WT1 encodes a zinc finger protein with a key role in urogenital development that is inactivated in a subset of Wilms' tumors. This tumor suppressor gene product contains an amino-terminal dimerization domain required for trans-inhibition of wild-type WT1 activity by mutants defective for DNA binding. In the course of ...

    journal_title：FEBS letters

    pub_type： 杂志文章

    doi：10.1016/0014-5793(96)00853-8

    authors： Bruening W,Moffett P,Chia S,Heinrich G,Pelletier J

    更新日期：1996-09-09 00:00:00

  • Nitric oxide mediates intracytoplasmic and intranuclear zinc release.

    abstract：:We previously described that NO. leads to destruction of ZnS clusters and release of Zn2+ from various proteins including zinc finger transcription factors. To assess the relevance in living cells, we investigated, whether exogenous NO. leads to an increase of cytoplasmic and nuclear free Zn2+. L929 cells, mouse splen...

    journal_title：FEBS letters

    pub_type： 杂志文章

    doi：10.1016/s0014-5793(97)00150-6

    authors： Berendji D,Kolb-Bachofen V,Meyer KL,Grapenthin O,Weber H,Wahn V,Kröncke KD

    更新日期：1997-03-17 00:00:00

  • Structures of the tumor necrosis factor alpha inducing protein Tipalpha: a novel virulence factor from Helicobacter pylori.

    abstract：:Helicobacter pylori secretes a unique virulence factor, Tipalpha, that enters gastric cells and both stimulates the production of the TNF-alpha and activates the NF-kappaB pathway. The structures of a truncated version of Tipalpha (TipalphaN34) in two crystal forms are presented here. Tipalpha adopts a novel beta(1)al...

    journal_title：FEBS letters

    pub_type： 杂志文章

    doi：10.1016/j.febslet.2009.04.033

    authors： Tosi T,Cioci G,Jouravleva K,Dian C,Terradot L

    更新日期：2009-05-19 00:00:00

  • Evolution of dnaQ, the gene encoding the editing 3' to 5' exonuclease subunit of DNA polymerase III holoenzyme in Gram-negative bacteria.

    abstract：:The nucleotide sequences of the dnaQ genes from Salmonella typhimurium and Buchnera aphidicola, encoding the epsilon-subunit of the DNA polymerase III holoenzyme, have been determined. The Salmonella typhimurium dnaQ protein consists of 243 amino acid residues with a calculated molecular weight of 27224. The Buchnera ...

    journal_title：FEBS letters

    pub_type： 杂志文章

    doi：10.1016/s0014-5793(96)01361-0

    authors： Huang Y,Braithwaite DK,Ito J

    更新日期：1997-01-02 00:00:00

  • Regulation of type V adenylyl cyclase by PMA-sensitive and -insensitive protein kinase C isoenzymes in intact cells.

    abstract：:Abstract Type V adenylyl cyclase (AC) was stably over-expressed in HEK293 cells (293AC-V). Forskolin-stimulated cAMP accumulation in 293AC-V was 5 times as great as that in control cells. PMA, a protein kinase C (PKC) activator, enhanced cAMP accumulation in 293AC-V cells dose-and time-dependently and this enhancement...

    journal_title：FEBS letters

    pub_type： 杂志文章

    doi：10.1016/0014-5793(96)00331-6

    authors： Kawabe Ji,Ebina T,Toya Y,Oka N,Schwencke C,Duzic E,Ishikawa Y

    更新日期：1996-04-22 00:00:00

  • The amino acid sequence of the zinc-requiring beta-lactamase II from the bacterium Bacillus cereus 569.

    abstract：:The amino acid sequence of the zinc-requiring beta-lactamase II from Bacillus cereus strain 569 has been determined. It consists of a single polypeptide chain of 227 residues. It is the only example so far fully characterized of a class B beta-lactamase, and is structurally and mechanistically distinct from both the w...

    journal_title：FEBS letters

    pub_type： 杂志文章

    doi：10.1016/0014-5793(85)81024-3

    authors： Ambler RP,Daniel M,Fleming J,Hermoso JM,Pang C,Waley SG

    更新日期：1985-09-23 00:00:00

  • The C-A mismatch base pair and the single-strand terminus in the E. coli initiator tRNA(fMet) acceptor stem adopt unusual conformations.

    abstract：:Acceptor stem variants of tRNA(fMet) (Escherichia coli) have been characterized by nuclear magnetic resonance. The wild type contains a C1-A72 mismatch pair which is crucial for its biological function. For comparison, the mismatch was replaced by regular pairs U1-A72 and C1-G72. Further variants contain an altered di...

    journal_title：FEBS letters

    pub_type： 杂志文章

    doi：10.1016/s0014-5793(00)01459-9

    authors： Zuleeg T,Vogtherr M,Schübel H,Limmer S

    更新日期：2000-04-28 00:00:00

  • Inhibition of ADP-ribosylation suppresses aberrant accumulation of lipidated apolipoprotein B in the endoplasmic reticulum.

    abstract：:ApoB-crescent, an endoplasmic reticulum (ER)-lipid droplet amalgamation structure, is a useful marker to indicate aberrant lipidated apolipoprotein B accumulation in the hepatocyte ER. Blockade of the ER-to-Golgi transport by either vesicle transport inhibitors or dominant-negative Arf1 caused a significant increase i...

    journal_title：FEBS letters

    pub_type： 杂志文章

    doi：10.1016/j.febslet.2013.09.036

    authors： Ohsaki Y,Cheng J,Yamairi K,Pan X,Hussain MM,Fujimoto T

    更新日期：2013-11-15 00:00:00

  • Characterisation of the expression and post-translational processing of the preprotachykinin-I gene and the regulated release of tachykinins by the RINm5F cell-line.

    abstract：:Normal transcription and postranslational processing of the preprotachykinin (PPT)-I gene and regulated release of substance P and neurokinin A by the rat pancreatic endocrine cell-line, RINm5F, has been demonstrated, using radioimmunoassays (RIAs), reversed-phase (rp)HPLC and Northern blot analysis. This is the first...

    journal_title：FEBS letters

    pub_type： 杂志文章

    doi：10.1016/0014-5793(92)80932-7

    authors： McGregor GP,Hartel R,Fehmann HC,Lankat-Buttgereit B,Göke B,Göke R,Voigt K

    更新日期：1992-11-09 00:00:00

  • Bacterial proteins carrying twin-R signal peptides are specifically targeted by the delta pH-dependent transport machinery of the thylakoid membrane system.

    abstract：:Glucose-fructose oxidoreductase (GFOR), a periplasmic protein of Zymomonas mobilis, is synthesized as a precursor polypeptide with a twin-R signal peptide for Sec-independent protein export in bacteria. In higher plant chloroplasts, twin-R signal peptides are specific targeting signals for the Sec-independent delta pH...

    journal_title：FEBS letters

    pub_type： 杂志文章

    doi：10.1016/s0014-5793(99)00269-0

    authors： Halbig D,Hou B,Freudl R,Sprenger GA,Klösgen RB

    更新日期：1999-03-19 00:00:00

  • Translational control of terminal oligopyrimidine mRNAs requires a specific regulator.

    abstract：:Terminal oligopyrimidine (TOP) mRNAs are a group of messengers translationally regulated according to the growth status of the cell. Two hypotheses have been proposed for the mechanism of the regulation: (i) there is a specific translational regulator which can reversibly alter TOP-mRNA structure, (ii) a component of ...

    journal_title：FEBS letters

    pub_type： 杂志文章

    doi：10.1016/s0014-5793(97)01209-x

    authors： Loreni F,Amaldi F

    更新日期：1997-10-27 00:00:00

  • Comparison of proline and N-methylnorleucine induced conformational equilibria in cyclic pentapeptides.

    abstract：:The cyclic, imido acid containing pentapeptides cyclo(Asp-Trp-(NMe)Nle-Asp-Phe) (cpp[NMeNle(3)]) and cyclo(Asp-Trp-Pro-Asp-Phe) (cpp[Pro(3)]) have been investigated by 1H-NMR spectroscopy in DMSO and by restrained molecular dynamics methods. The spectra indicate the existence of at least four cis/trans isomers for cpp...

    journal_title：FEBS letters

    pub_type： 杂志文章

    doi：10.1016/0014-5793(96)00469-3

    authors： Weisshoff H,Wieprecht T,Henklein P,Frömmel C,Antz C,Mügge C

    更新日期：1996-06-03 00:00:00

  • Structure and thermal stability of the extracellular fragment of human transferrin receptor at extracellular and endosomal pH.

    abstract：:Fourier transform infrared spectroscopy has been used to study the solution structure and thermal stability of the extracellular fragment of human transferrin receptor (tfRt) at extracellular and endosomal pH. At extracellular pH tfRt is composed of 56% alpha-helix, 19% beta-sheet and 14% turns. Upon acidification to ...

    journal_title：FEBS letters

    pub_type： 杂志文章

    doi：10.1016/0014-5793(94)00774-8

    authors： Hadden JM,Bloemendal M,Haris PI,van Stokkum IH,Chapman D,Srai SK

    更新日期：1994-08-22 00:00:00

  • Targeting of the MET receptor tyrosine kinase by small molecule inhibitors leads to MET accumulation by impairing the receptor downregulation.

    abstract：:The MET receptor tyrosine kinase is deregulated primarily via overexpression or point mutations in various human cancers and different strategies for MET inhibition are currently evaluated in clinical trials. We observed by Western blot analysis and by Flow cytometry that MET inhibition by different MET small molecule...

    journal_title：FEBS letters

    pub_type： 杂志文章

    doi：10.1016/j.febslet.2013.12.025

    authors： Leiser D,Pochon B,Blank-Liss W,Francica P,Glück AA,Aebersold DM,Zimmer Y,Medová M

    更新日期：2014-03-03 00:00:00

  • Differences in polypeptide composition and enzyme activity between cold-stable and cold-labile microtubules and study of microtubule alkaline phosphatase activity.

    abstract：:Cold-stable and cold-labile microtubules were prepared by two cycles of assembly and disassembly and two periods of exposure to cold. The cold-labile preparations were shown to contain a higher proportion of a high molecular mass microtubule-associated protein (MAP 2) than cold-stable preparations. The cold-stable pre...

    journal_title：FEBS letters

    pub_type： 杂志文章

    doi：10.1016/0014-5793(84)80341-5

    authors： Hesketh JE

    更新日期：1984-04-24 00:00:00

  • Transcriptional activator, AoXlnR, mediates cellulose-inductive expression of the xylanolytic and cellulolytic genes in Aspergillus oryzae.

    abstract：:AoXlnR was isolated as a transcriptional activator of the major xylanase gene, xynF1, in Aspergillus oryzae. To investigate the spectrum of genes under the control of AoXlnR, expression of the xylanolytic and cellulolytic genes in an A. oryzae wild type strain, an AoxlnR disruptant and an AoXlnR overexpressed strain w...

    journal_title：FEBS letters

    pub_type： 杂志文章

    doi：10.1016/s0014-5793(02)03328-8

    authors： Marui J,Kitamoto N,Kato M,Kobayashi T,Tsukagoshi N

    更新日期：2002-09-25 00:00:00

  • The effect of haloperidol on D2 dopamine receptor subtype mRNA levels in the brain.

    abstract：:Chronic neuroleptic treatment induces an increase in the density of D2 dopamine receptors in the striatum. The effect of prolonged administration of haloperidol on mRNA levels of the short (D2S) and long (D2L) isoforms of the D2 dopamine receptor and the D3 dopamine receptor in different brain regions was examined. Ha...

    journal_title：FEBS letters

    pub_type： 杂志文章

    doi：10.1016/0014-5793(94)80385-4

    authors： Fishburn CS,David C,Carmon S,Fuchs S

    更新日期：1994-02-14 00:00:00

  • Vapor-phase modification of sulfhydryl groups in proteins.

    abstract：:Proteins and peptides are readily and specifically modified at their sulfhydryl groups by the vapors of a mixture of 4-vinylpyridine and tributylphosphine. The phenylthiohydantoin derivative of S-beta-(4-pyridylethyl)cysteine formed during sequence analysis is easily detectable in current identification systems. ...

    journal_title：FEBS letters

    pub_type： 杂志文章

    doi：10.1016/0014-5793(87)81558-2

    authors： Amons R

    更新日期：1987-02-09 00:00:00

  • Evidence for a novel phosphopantetheinyl transferase domain in the polyketide synthase for enediyne biosynthesis.

    abstract：:The polyketide synthase associated with the biosynthesis of enediyne-containing calicheamicin contains a putative phosphopantetheinyl transferase (PPTase) domain. By cloning and expressing the C-terminal region of the polyketide synthase and in vitro phosphopantetheinylation assay, we found that the PPTase domain exhi...

    journal_title：FEBS letters

    pub_type： 杂志文章

    doi：10.1016/j.febslet.2008.02.061

    authors： Murugan E,Liang ZX

    更新日期：2008-04-02 00:00:00

  • Semliki Forest virus vectors: efficient vehicles for in vitro and in vivo gene delivery.

    abstract：:Rapidly generated high-titer Semliki Forest virus (SFV) vectors can infect numerous mammalian cell lines and primary cell cultures, and result in high levels of transgene expression. SFV-based expression of transmembrane receptors has been characterized by specific ligand-binding activity and functional responses. Ada...

    journal_title：FEBS letters

    pub_type： 杂志文章,评审

    doi：10.1016/s0014-5793(01)02707-7

    authors： Lundstrom K,Schweitzer C,Rotmann D,Hermann D,Schneider EM,Ehrengruber MU

    更新日期：2001-08-31 00:00:00

  • Is the acetylcholine releasing protein mediatophore present in rat brain?

    abstract：:Mediatophore is a protein purified from the nerve terminal membranes of Torpedo electric organ. It confers to artificial membranes a calcium-dependent mechanism that translocates acetylcholine. When similar reconstitution experiments are applied to rat brain synaptosomal membranes they reveal the presence of mediatoph...

    journal_title：FEBS letters

    pub_type： 杂志文章

    doi：10.1016/0014-5793(88)80475-7

    authors： Israël M,Lesbats B,Morel N,Manaranche R,Le Gal la Salle G

    更新日期：1988-06-20 00:00:00

  • Simulation of multihaem cytochromes.

    abstract：:This article presents an overview of the simulation studies of the behaviour of multihaem cytochromes using theoretical/computational methodologies, with an emphasis on cytochrome c(3). It starts with the first studies using rigid molecules and continuum electrostatic models, where protonation and redox events were tr...

    journal_title：FEBS letters

    pub_type： 杂志文章,评审

    doi：10.1016/j.febslet.2011.10.019

    authors： Soares CM,Baptista AM

    更新日期：2012-03-09 00:00:00

  • Monoclonal antibody evidence for structural similarities between the central rod regions of actinin and dystrophin.

    abstract：:A monoclonal antibody, MANDYS141, binds to both dystrophin and actinin on Western blots (SDS-denatured), but only to actinin in frozen sections of human muscle (native conformation). It differs from a polyclonal cross-reacting antiserum in that it binds to several muscle isoforms of actinin (smooth, fast and slow) fro...

    journal_title：FEBS letters

    pub_type： 杂志文章

    doi：10.1016/0014-5793(90)80460-z

    authors： Nguyen TM,Ellis JM,Ginjaar IB,van Paassen MM,van Ommen GJ,Moorman AF,Cartwright AJ,Morris GE

    更新日期：1990-10-15 00:00:00

  • Limited proteolysis of the coxsackievirus and adenovirus receptor (CAR) on HeLa cells exposed to trypsin.

    abstract：:Trypsin treatment of HeLa cells results in a limited proteolysis of the coxsackievirus and adenovirus receptor (CAR) after which the cleaved CAR remains cell-associated and tryptic peptides remain associated through disulfide bonds. Trypsin-treated HeLa cells remain susceptible to infection with coxsackievirus B and p...

    journal_title：FEBS letters

    pub_type： 杂志文章

    doi：10.1016/s0014-5793(00)02144-x

    authors： Carson SD

    更新日期：2000-11-03 00:00:00

  • Molecular cloning and sequence analysis of cDNA encoding human cholesterol 7 alpha-hydroxylase.

    abstract：:A complete cDNA clone encoding human cholesterol 7 alpha-hydroxylase has been isolated using a rat P-450ch7 alpha cDNA insert [(1989) FEBS Lett. 257, 97-100] as a probe and totally sequenced. The cDNA contained 1512-base pair open reading frame encoding 504 amino acid residues (Mr 57,630), 39-base pair 5'-untranslated...

    journal_title：FEBS letters

    pub_type： 杂志文章

    doi：10.1016/0014-5793(90)80992-r

    authors： Noshiro M,Okuda K

    更新日期：1990-07-30 00:00:00

  • Modulation of cofactor requirement for the activation of protein kinase C by heparin. Possible effect at the regulatory domain.

    abstract：:Heparin was found to stimulate the phosphorylation of histone H1 but not protamine sulfate catalyzed by Ca2+/phospholipid-dependent protein kinase (protein kinase C or PKC). The effect of heparin on histone H1 phosphorylation appeared to be due to an increase in phosphatidylserine affinity for PKC activation in the pr...

    journal_title：FEBS letters

    pub_type： 杂志文章

    doi：10.1016/0014-5793(91)80533-9

    authors： Goueli SA,Hanten JA,Ahmed K

    更新日期：1991-05-06 00:00:00

  • Histone H5 and H1 cross-reacting material is restricted to erythroid cells in chicken.

    abstract：:Monoclonal H5 antibodies and a polyclonal antiserum, raised against the globular domain of chicken H5 (GH5) but which cross-reacts with histone H1(0) from mouse liver, were used to search for H5 or H1 (0)-like proteins in chicken embryo and adult tissue sections by indirect immunofluorescence. Chicken cell lines in cu...

    journal_title：FEBS letters

    pub_type： 杂志文章

    doi：10.1016/0014-5793(85)80704-3

    authors： Shannon MF,Wigley PL,Wells JR

    更新日期：1985-07-08 00:00:00

  • Isolation of a brain peptide identical to the intestinal PHI (peptide HI).

    abstract：:The isolation of a brain peptide identical to the intestinal peptide PHI (peptide HI) is described. The peptide was isolated from porcine brain extract using a chemical assay method based on its C-terminal isoleucine amide structure. The complete amino acid sequence of the peptide was found to be: His-Ala-Asp-Gly-Val-...

    journal_title：FEBS letters

    pub_type： 杂志文章

    doi：10.1016/0014-5793(83)80617-6

    authors： Tatemoto K,Carlquist M,McDonald TJ,Mutt V

    更新日期：1983-03-21 00:00:00

  • Effect of new antioxidant cysteinyl-flavanol conjugates on skin cancer cells.

    abstract：:Novel catechin derivatives obtained from grape procyanidins and l-cysteine scavenge free radicals by hydrogen atom donation, rather than electron transfer, and reduce cell viability in A375 and M21 melanoma cells. In particular, 4beta-(S-cysteinyl)epicatechin 3-O-gallate has a free radical scavenging capacity as stron...

    journal_title：FEBS letters

    pub_type： 杂志文章

    doi：10.1016/j.febslet.2005.06.051

    authors： Lozano C,Torres JL,Julià L,Jimenez A,Centelles JJ,Cascante M

    更新日期：2005-08-15 00:00:00

  • Cloning and characterization of a novel human pH-dependent organic cation transporter, OCTN1.

    abstract：:cDNA for a novel proton/organic cation transporter, OCTN1, was cloned from human fetal liver and its transport activity was investigated. OCTN1 encodes a 551-amino acid protein with 11 transmembrane domains and one nucleotide binding site motif. It is strongly expressed in kidney, trachea, bone marrow and fetal liver ...

    journal_title：FEBS letters

    pub_type： 杂志文章

    doi：10.1016/s0014-5793(97)01441-5

    authors： Tamai I,Yabuuchi H,Nezu J,Sai Y,Oku A,Shimane M,Tsuji A

    更新日期：1997-12-08 00:00:00