Identification of nuclear localization signals within the zinc fingers of the WT1 tumor suppressor gene product.

abstract：:The interactions between clavulanate and three class C enzymes have been studied in detail. In all cases, the reactions followed branched pathways where 25-150 turnovers occurred before inactivation was completed. Reactivation rates were quite low. The poor efficiency of clavulanate as a class C inactivator appeared t...

journal_title：FEBS letters

authors： Monnaie D,Frere JM

更新日期：1993-11-22 00:00:00

abstract：:Using a panel of monoclonal antibodies, it has previously been demonstrated that the cytosol of nucleated red cells (trout and turkey) contains a protein similar to arrestin, a soluble protein found so far only in the photosensitive cells and which, by binding to photoexcited rhodopsin, inhibits the phototransduction ...

journal_title：FEBS letters

authors： Scheuring U,Franco M,Fievet B,Guizouarn H,Mirshahi M,Faure JP,Motais R

更新日期：1990-12-10 00:00:00

abstract：:The activity of breast milk BSDL was assayed with or without phospholipids as extra-intestinal effector candidates. Phosphatidic acid, lysophosphatidic acid and platelet activating factor but not phosphatidylcholine and lysophosphatidylcholine stimulated BSDL activity at least as efficiently as taurocholate. The appar...

journal_title：FEBS letters

authors： Fontbonne H,Puigserver A,Bouza B,Lombardo D,Ajandouz el H

更新日期：2013-09-17 00:00:00

abstract：:Using RT-PCR we distinguished mRNAs for all known G-protein coupled serotonin receptors expressed in various rat and porcine blood vessels. Nearly all vessels expressed 5HT1D beta, 5-HT2A, 5-HT2B, 5-HT4, and 5-HT7 receptor mRNA to different extents. New splice variants of the porcine 5-HT4 receptor were observed. Simi...

journal_title：FEBS letters

authors： Ullmer C,Schmuck K,Kalkman HO,Lübbert H

更新日期：1995-08-21 00:00:00

abstract：:The effects of protein disulfide isomerase (PDI) on the four structured des species that accumulate in the rate-determining steps of ribonuclease A folding were investigated at pH 8.0 and 15 degrees C. The results indicate that PDI catalyzes the conversion of the kinetically trapped intermediates, des-[26-84] and des-...

journal_title：FEBS letters

authors： Shin HC,Song MC,Scheraga HA

更新日期：2002-06-19 00:00:00

abstract：:The G-protein regulatory (GPR) motif is a approximately 25 amino acid sequence that stabilizes the GDP-bound conformation of Gialpha. To identify additional GPR motifs, we expanded a motif-based search strategy and identified an additional 4 mammalian proteins (WAVE1-3, rat GHRH) and 10 plant proteins with candidate G...

journal_title：FEBS letters

authors： Song KS,Peterson YK,Freidman A,Blumer JB,Sato M,Lanier SM

更新日期：2006-04-03 00:00:00

abstract：:Protein D2 forms the water-filled pore across the outer membrane of Pseudomonas aeruginosa and allows the penetration of imipenem. We cloned the protein D2 gene by the antibody screening technique. When the imipenem-resistant mutant lacking protein D2 harbored the plasmid with the cloned D2 gene, the mutant overproduc...

journal_title：FEBS letters

authors： Yoneyama H,Nakae T

更新日期：1991-06-03 00:00:00

abstract：:We have explored the mechanisms involved in the facilitation of glutamate release mediated by the activation of kainate receptors (KARs) in the cortex using isolated nerve terminals (synaptosomes). Kainate (KA) produced an increase on glutamate release at 100 μM. The effect of KA was antagonized by NBQX (with AMPA rec...

journal_title：FEBS letters

authors： Rodríguez-Moreno A,Sihra TS

更新日期：2013-03-18 00:00:00

abstract：:Disulfide bridges were introduced into CrylAa, a Bacillus thuringiensis lepidopteran toxin, to stabilize different protein domains including domain I alpha-helical regions thought to be involved in membrane integration and permeation. Bridged mutants could not form functional ion channels in lipid bilayers in the oxid...

journal_title：FEBS letters

authors： Schwartz JL,Juteau M,Grochulski P,Cygler M,Préfontaine G,Brousseau R,Masson L

更新日期：1997-06-30 00:00:00

abstract：:While the majority of studies have focused on the biological roles of heparin-binding proteins, relatively little is known about their key residues and structural elements responsible for heparin interaction. In this study, we employed the IgG-binding domain B1 of Streptococcal protein G as a miniature scaffold to inv...

journal_title：FEBS letters

authors： Cheng YY,Cheng CS,Lee TR,Chang WS,Lyu PC

更新日期：2016-09-01 00:00:00

abstract：:Using a variety of peptide analogues of oxytocin (OT) and Arg8-vasopressin (AVP), OT-mediated induction of urokinase-type plasminogen activator (uPA) was examined in LLC-PK1 renal epithelial cells, which possess distinct high-affinity receptors of both the OT- and vasopressin renal (V2-) types. OT or OT-receptor speci...

journal_title：FEBS letters

authors： Jans DA,Pavo I,Fahrenholz F

更新日期：1993-01-04 00:00:00

abstract：:The characteristics of renal transport of L-alanine by luminal-membrane vesicles from proximal straight tubules (pars recta) of rabbit kidney were investigated. The following picture emerges from transport studies. Two electrogenic and Na+ requiring systems confined to this region of the nephron exist for the transpor...

journal_title：FEBS letters

authors： Vorum H,Jessen H,Jørgensen KE,Sheikh MI

更新日期：1988-01-18 00:00:00

abstract：:Pyoverdine I (PVDI) is the major siderophore produced by Pseudomonas aeruginosa to import iron. Biosynthesis of this chelator involves non-ribosomal peptide synthetases and other enzymes. PvdQ is a periplasmic enzyme from the NTN hydrolase family and is involved in the final steps of PVDI biosynthesis. A pvdQ mutant p...

journal_title：FEBS letters

authors： Hannauer M,Schäfer M,Hoegy F,Gizzi P,Wehrung P,Mislin GL,Budzikiewicz H,Schalk IJ

更新日期：2012-01-02 00:00:00

abstract：:The roles of 2'-OH groups in the binding of mRNA to human ribosomes were studied using site-directed cross-linking. We found that both mRNA and mDNA analogues bearing a cross-linker can modify ribosomal proteins (rps) S3e and S2e at the mRNA entry site independently on tRNA presence, but only mRNA analogues were capab...

journal_title：FEBS letters

authors： Graifer D,Zhigailov A,Ven'yaminova A,Malygin A,Iskakov B,Karpova G

更新日期：2012-10-19 00:00:00

abstract：:Ca(2+) entering cells through store-operated channels (SOCs) affects most cell functions, and excess SOC is associated with pathologies. The molecular makeup of SOCs and their mechanisms of gating were clarified with the discovery of the Orais and STIM1. Another form of SOCs are the TRPCs. STIM1 gates both Orai and TR...

journal_title：FEBS letters

authors： Lee KP,Yuan JP,Hong JH,So I,Worley PF,Muallem S

更新日期：2010-05-17 00:00:00

abstract：:Using conserved fingerprints in the glycosyltransferase (GTase) domain of high-molecular-weight penicillin-binding proteins (PBP), a gene (mgt) encoding a putative monofunctional glycosyltransferase has been identified in Haemophilus influenzae and in other bacteria] species. Here we report the cloning of the homologo...

journal_title：FEBS letters

authors： Di Berardino M,Dijkstra A,Stüber D,Keck W,Gubler M

更新日期：1996-08-26 00:00:00

abstract：:Synaptotagmins (Syts) are involved in Ca(2+)-dependent insulin release. However, which Syt isoform is functional in primary beta-cells remains unknown. We demonstrate by electron microscopy of pancreatic islets, the association of Syt 9 with insulin granules. Silencing of Syt 9 by RNA interference adenovirus in islet ...

journal_title：FEBS letters

authors： Iezzi M,Eliasson L,Fukuda M,Wollheim CB

更新日期：2005-09-26 00:00:00

abstract：:We used cDNA microarray to identify transforming growth factor beta (TGF-beta) responsive target genes during osteoblast development and found that nephronectin (Npnt) is one such gene that is significantly down-regulated. Here we report the role of TGF-beta in regulating Npnt-mediated osteoblast differentiation. We f...

journal_title：FEBS letters

authors： Fang L,Kahai S,Yang W,He C,Seth A,Peng C,Yang BB

更新日期：2010-07-02 00:00:00

abstract：:Glutamate uptake by rat hepatocytes in primary monolayer culture was found to be mediated by a Na+-independent and by two Na+-dependent transport systems of high and low affinity. Inhibition studies with cysteate and other model amino acids rules out the participation of the neutral amino acid transport systems A, ASC...

journal_title：FEBS letters

authors： Gebhardt R,Mecke D

更新日期：1983-09-19 00:00:00

abstract：:The physical properties of amino acids were investigated in order to evaluate their possible relationship to the assignment of codons for amino acids in the genetic code. A comparison of the interconversion probability between amino acids and the distances between the amino acids for individual physical properties rev...

journal_title：FEBS letters

authors： Sitaramam V

更新日期：1989-04-10 00:00:00

abstract：:The mechanisms that underlie axon formation are still poorly understood. GSK3 has been recently implicated in establishing the axon and in its elongation. We have used four different GSK3 inhibitors to determine the role of GSK3 activity in hippocampal neurons at different periods of time. Inhibition of GSK3 activity ...

journal_title：FEBS letters

authors： Garrido JJ,Simón D,Varea O,Wandosell F

更新日期：2007-04-17 00:00:00

abstract：:SufC is an ATPase component of the SUF machinery, which is involved in the biosynthesis of Fe-S clusters. To gain insight into the function of this protein, we have determined the crystal structure of Escherichia coli SufC at 2.5A resolution. Despite the similarity of the overall structure with ABC-ATPases (nucleotide...

journal_title：FEBS letters

authors： Kitaoka S,Wada K,Hasegawa Y,Minami Y,Fukuyama K,Takahashi Y

更新日期：2006-01-09 00:00:00

abstract：:Three major UDP-glucuronyltransferase isoenzymes (50-54 kDa) have been identified by immunoblot analysis. Bilirubin UDP-glucuronyltransferase (54 kDa) was specially induced by treatment of the rats with clofibrate. This isoenzyme was not detectable in liver microsomal extracts from congenitally jaundiced Gunn rats and...

journal_title：FEBS letters

authors： Scragg I,Celier C,Burchell B

更新日期：1985-04-08 00:00:00

abstract：:Exposure of HeLa and HL60 cells to sodium arsenite or cadmium chloride led to marked increases in cellular heme oxygenase activity. SDS-polyacrylamide gel electrophoresis of [35S]methionine-labeled cellular proteins indicated that these treatments also resulted in the induction of a 32-kDa protein. Immunoblot analysis...

journal_title：FEBS letters

authors： Taketani S,Kohno H,Yoshinaga T,Tokunaga R

更新日期：1989-03-13 00:00:00

abstract：:The Na+-induced release of accumulated Ca2+ from heart mitochondria is inhibited by amiloride, benzamil and several other amiloride analogues. These drugs do not affect uptake or release of Ca2+ mediated by the ruthenium red-sensitive uniporter and their effects, like those of diltiazem and other Ca2+-antagonists, app...

journal_title：FEBS letters

authors： Jurkowitz MS,Altschuld RA,Brierley GP,Cragoe EJ Jr

更新日期：1983-10-17 00:00:00

abstract：:Formylmethanofuran dehydrogenase, a key enzyme of methanogenesis, was purified 100-fold from methanol grown Methanosarcina barkeri to apparent homogeneity and a specific activity of 34 mumol.min-1.mg protein-1. Molybdenum was found to co-migrate with the enzyme activity. The molybdenum content of purified preparations...

journal_title：FEBS letters

authors： Karrasch M,Börner G,Enssle M,Thauer RK

更新日期：1989-08-14 00:00:00

abstract：:We propose a dynamic model for the competition between exon-exon ligation and 3'-end hydrolysis valid for sequentially folded pre-mRNA introns of group I. This model accounts for the delay in the formation of conserved helix P10 until the 5' exon has been cleaved, a requirement to prevent hydrolysis at the 3' end of t...

journal_title：FEBS letters

authors： Fernández A

更新日期：1992-02-03 00:00:00

abstract：:The rate at which dinucleoside phosphates are cleaved by RNases is supposed to be determined by the mole fraction of enzyme-substrate complexes in which the phosphodiester moiety of a dinucleoside phosphate has a highly reactive conformation. The mole fraction of such complexes for a particular RNase depends on the na...

journal_title：FEBS letters

authors： Yakovlev GI,Bocharov AL,Moiseyev GP,Mikhaylov SN

更新日期：1985-01-07 00:00:00

abstract：:The effect of removal of Ca2+ bound to thrombospondin (TSP) on the protein structure in solution has been investigated using ESR spin-label techniques. A maleimide spin label was selectively attached to the free thiol group presumably near the carboxyl-terminal domain in which Ca2+-binding sites are situated. The ESR ...

journal_title：FEBS letters

authors： Slane JM,Mosher DF,Lai CS

更新日期：1988-03-14 00:00:00

abstract：:Curcumin, the active ingredient of the rhizome of Curcuma longa has anti-inflammatory, antioxidant and antiproliferative activities. Although its precise mode of action remains elusive, studies have shown that chemopreventive action of curcumin might be due to its ability to induce apoptosis in cancer cells. Curcumin ...

journal_title：FEBS letters

authors： Bhaumik S,Anjum R,Rangaraj N,Pardhasaradhi BV,Khar A

更新日期：1999-08-06 00:00:00