Abstract:
:Using conserved fingerprints in the glycosyltransferase (GTase) domain of high-molecular-weight penicillin-binding proteins (PBP), a gene (mgt) encoding a putative monofunctional glycosyltransferase has been identified in Haemophilus influenzae and in other bacteria] species. Here we report the cloning of the homologous Escherichia coli gene and show that the solubilised membrane fraction of E. coli cells overexpressing the mgt gene contain a significantly increased peptidoglycan synthesis activity. In contrast to the high-molecular-weight PBPs, this activity is not inhibited by Flavomycin.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Di Berardino M,Dijkstra A,Stüber D,Keck W,Gubler Mdoi
10.1016/0014-5793(96)00809-5subject
Has Abstractpub_date
1996-08-26 00:00:00pages
184-8issue
2eissn
0014-5793issn
1873-3468pii
0014579396008095journal_volume
392pub_type
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