Abstract:
:The roles of 2'-OH groups in the binding of mRNA to human ribosomes were studied using site-directed cross-linking. We found that both mRNA and mDNA analogues bearing a cross-linker can modify ribosomal proteins (rps) S3e and S2e at the mRNA entry site independently on tRNA presence, but only mRNA analogues were capable of a tRNA(Phe)-dependent binding to human ribosomes and cross-linking to rpS26e in the mRNA binding centre. Thus, 2'-OH groups of mRNA are unimportant for binding at the entry site but they are crucial for codon-anticodon interactions at the P site, implying the existence of mRNA-ribosome contacts that do not occur in bacteria.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Graifer D,Zhigailov A,Ven'yaminova A,Malygin A,Iskakov B,Karpova Gdoi
10.1016/j.febslet.2012.09.003subject
Has Abstractpub_date
2012-10-19 00:00:00pages
3731-6issue
20eissn
0014-5793issn
1873-3468pii
S0014-5793(12)00705-3journal_volume
586pub_type
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