Abstract:
:When MCF-7 cells were treated with 17 beta-estradiol, dexamethasone, or promegestone at 37 degrees C, the KD of receptors for their cognate ligands was found to decrease as compared to that measured at 2 degrees C. Cell incubation with hormone at 37 degrees C did not affect the Bmax of glucocorticoid and progesterone receptors, but caused a 40% increase of that of estrogen receptor. This increase required the presence of ligand, was insensitive to cycloheximide, and was completed within 10 min of cell incubation at physiological temperature. We conclude that an early step of estrogen action is the recruitment of pre-existing receptor molecules through activation of their binding capacity.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Rossini GP,Malaguti Cdoi
10.1016/0014-5793(95)01264-6subject
Has Abstractpub_date
1995-12-04 00:00:00pages
151-4issue
3eissn
0014-5793issn
1873-3468pii
0014-5793(95)01264-6journal_volume
376pub_type
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