Primary structures of two ribonucleases from ginseng calluses. New members of the PR-10 family of intracellular pathogenesis-related plant proteins.

abstract：:Using crude membrane preparations of Saccharomyces cerevisiae, we have demonstrated that glucose and glucose analogues which are not efficiently phosphorylated activate the guanine nucleotide-dependent adenylate cyclase in vitro. The activation appears to be mediated by the Ras proteins. Moreover, data are presented i...

journal_title：FEBS letters

authors： Pardo LA,Sánchez LM,Lazo PS,Ramos S

更新日期：1991-09-23 00:00:00

abstract：:The biosynthesis of human tumor necrosis factor (hTNF) was studied. The amino-terminal extension of the hTNF precursor (26 kDa polypeptide) was not cleaved off in a cell-free system supplemented with dog pancreas microsomes. Correct maturation of pre-hTNF was nevertheless not restricted to the macrophage system: in th...

journal_title：FEBS letters

authors： Müller R,Marmenout A,Fiers W

更新日期：1986-03-03 00:00:00

abstract：:For almost 30 years, Wnt proteins have been known as key regulators of many developmental decisions, including the formation of the embryonic axes, patterning of the CNS, limb bud outgrowth and segment polarity. However, live cell imaging of active Wnt proteins was rarely reported. Here, we have generated a Wnt2b-EGFP...

journal_title：FEBS letters

authors： Holzer T,Liffers K,Rahm K,Trageser B,Ozbek S,Gradl D

更新日期：2012-06-04 00:00:00

abstract：:We examined the role of p38 mitogen-activated protein (MAP) kinase in the tumor necrosis factor alpha (TNF-alpha)- or interleukin-1beta (IL-1beta)-induced production of interleukin-6 (IL-6) and interleukin-8 (IL-8) in fresh rheumatoid synovial fibroblast (RSF) cultures concomitantly with the induction of p38 MAP kinas...

journal_title：FEBS letters

authors： Suzuki M,Tetsuka T,Yoshida S,Watanabe N,Kobayashi M,Matsui N,Okamoto T

更新日期：2000-01-07 00:00:00

abstract：:Connexin 43 (Cx43) hemichannels may form open channels in the plasma membrane when exposed to specific stimuli, e.g. reduced extracellular concentration of divalent cations, and allow passage of fluorescent molecules and presumably a range of smaller physiologically relevant molecules. However, the permeability profil...

journal_title：FEBS letters

authors： Hansen DB,Braunstein TH,Nielsen MS,MacAulay N

更新日期：2014-04-17 00:00:00

abstract：:The neurogenic gene Drosophila big brain (bib) has a high sequence homology to aquaporin-4. However, its cellular functions in Drosophila neurogenesis have remained elusive. Here we investigated cell adhesion, and the ion and water permeability of Bib. The adhesive function was examined by a cell aggregation assay usi...

journal_title：FEBS letters

authors： Tatsumi K,Tsuji S,Miwa H,Morisaku T,Nuriya M,Orihara M,Kaneko K,Okano H,Yasui M

更新日期：2009-06-18 00:00:00

abstract：:The mediator complex in the RNA polymerase II holoenzyme is known to be involved in transcriptional activation. The role of the essential mediator component Srb7p has been difficult to investigate, since no conditional lethal allele has been available to date. While the expression of Srb7p under the control of a repre...

journal_title：FEBS letters

authors： Gromöller A,Lehming N

更新日期：2000-10-27 00:00:00

abstract：:We have used 19F NMR and CD spectra to study interactions of TFP with CaM under various conditions where no aggregation of TFP occurs. It was found that TFP is bound to CaM in the absence of Ca2+ with Ka greater than 4 X 10(4) M in terms of 19F NMR of TFP and that KCl markedly influences the interaction of TFP with Ca...

journal_title：FEBS letters

authors： Shimizu T,Hatano M

更新日期：1983-08-22 00:00:00

abstract：:The aim of the present research was to analyze the pathways for phosphatidic acid metabolism in purified nuclei from liver. Lipid phosphate phosphatase, diacylglycerol lipase, monoacylglycerol lipase and PA-phospholipase type A activities were detected. The presence of lysophosphatidic acid significantly reduced DAG p...

journal_title：FEBS letters

authors： Gaveglio VL,Pasquaré SJ,Giusto NM

更新日期：2013-04-02 00:00:00

abstract：:We have identified and purified a DNA binding protein which specifically binds to a segment of the mouse alpha 2 (I) collagen promoter between -420 and -399 bp upstream of the start of transcription. Purification included heparin-agarose and sequence-specific DNA-affinity chromatography, followed by SDS-PAGE and renat...

journal_title：FEBS letters

authors： Hatamochi A,de Crombrugghe B,Krieg T

更新日期：1993-08-02 00:00:00

abstract：:Advanced glycation end products (AGE) are known to serve as ligands for the scavenger receptors such as SR-A, CD36 and SR-BI. In the current study, we examined whether AGE is recognized by lectin-like oxidized low density lipoprotein receptor-1 (LOX-1). Cellular binding experiments revealed that AGE-bovine serum album...

journal_title：FEBS letters

authors： Jono T,Miyazaki A,Nagai R,Sawamura T,Kitamura T,Horiuchi S

更新日期：2002-01-30 00:00:00

abstract：:Since the discovery of RNAi, its mechanism in plants and animals has been intensively studied, widely exploited as a research tool, and used for a number of potential commercial applications. In this article, we discuss the platforms for delivering RNAi in plants. We provide a brief background to these platforms and c...

journal_title：FEBS letters

authors： Watson JM,Fusaro AF,Wang M,Waterhouse PM

更新日期：2005-10-31 00:00:00

abstract：:The morphology of complexes between relaxed circular plasmid pBR322 DNA and tripeptide L-Val-L-Val-L-Val-NH-NH-Dns (TVP) at different peptide/DNA ratios was studied by electron microscopy. The results show that interaction of TVP with circular DNA leads to the formation of perfect torus-shaped particles. The torus par...

journal_title：FEBS letters

authors： Vengerov YY,Semenov TE,Streltsov SA,Makarov VL,Khorlin AA,Gursky GV

更新日期：1985-01-21 00:00:00

abstract：:The alpha-subunit of the TRAP complex (TRAP alpha) is a single-spanning membrane protein of the endoplasmic reticulum (ER) which is found in proximity of nascent polypeptide chains translocating across the membrane. Here, we demonstrate the widespread occurrence of TRAP alpha in eukaryotes as indicated by its existenc...

journal_title：FEBS letters

authors： Hartmann E,Prehn S

更新日期：1994-08-08 00:00:00

abstract：:The importance of the individual amino acid residues of AIP (KKALRRQEAVDAL), a highly specific inhibitor of calmodulin-dependent protein kinase II (CaMKII), was studied. Replacement of Arg6, Gln7, or Ala9 by other amino acid residues produced a marked increase in the IC50 value. Leu4 and Val10 were also sensitive to r...

journal_title：FEBS letters

authors： Ishida A,Shigeri Y,Tatsu Y,Uegaki K,Kameshita I,Okuno S,Kitani T,Yumoto N,Fujisawa H

更新日期：1998-05-01 00:00:00

abstract：:Creatine kinases (CK) play a prominent role in cell energy distribution through an energy shuttle between mitochondria and other organelles. Human brain CK was cloned and overexpressed in COS-7 cells. We then deleted His-65 and/or Pro-66 situated near the center of a flexible loop as shown by X-ray crystallography on ...

journal_title：FEBS letters

authors： Mourad-Terzian T,Steghens JP,Min KL,Collombel C,Bozon D

更新日期：2000-06-09 00:00:00

abstract：:Extracellular ATP itself elicited the generation of superoxide (O2-) in guinea pig peritoneal macrophages associated with an increase in cytosolic calcium ([Ca2+]i). The ATP-induced O2- generation was completely inhibited by pretreatment with pertussis toxin (PT) accompanied by the suppression of [Ca2+]i mobilization....

journal_title：FEBS letters

authors： Nakanishi M,Takihara H,Minoru Y,Yagawa K

更新日期：1991-04-22 00:00:00

abstract：:In mammalian cells, the Bcl-2 and Bcl-x(L) proteins suppress programmed cell death whereas the topographically similar Bax protein accelerates the apoptotic process. Recently published data suggest that expression of the human Bax-alpha gene is lethal for the yeast Saccharomyces cerevisiae and that this toxicity can b...

journal_title：FEBS letters

authors： Greenhalf W,Stephan C,Chaudhuri B

更新日期：1996-02-12 00:00:00

abstract：:SUPERMAN was identified as a putative regulator of transcription that acts in floral development, but its function remains to be clarified. We demonstrate here that SUPERMAN is an active repressor whose repression domain is located in the carboxy-terminal region. Ectopic expression of SUPERMAN that lacked the repressi...

journal_title：FEBS letters

authors： Hiratsu K,Ohta M,Matsui K,Ohme-Takagi M

更新日期：2002-03-13 00:00:00

abstract：:An erythroid-specific enhancer was previously identified in the 3'-flanking region of the beta adult gene in chicken and duck, by transfection into AEV transformed chicken erythroblasts. Here we show that the duck enhancer is equally active in erythroid human K562 cells, presenting an embryonic/fetal program of globin...

journal_title：FEBS letters

authors： Kretsovali A,Marcaud L,Huesca M,Scherrer K

更新日期：1988-07-18 00:00:00

abstract：:Secretoneurin is a recently characterized neuropeptide present in the primary amino acid sequence of secretogranin II. We investigated the proteolytic processing of secretogranin II by prohormone convertases in vivo in a cellular system using the vaccinia virus system. Both PC1 and PC2 can cleave the secretogranin II ...

journal_title：FEBS letters

authors： Hoflehner J,Eder U,Laslop A,Seidah NG,Fischer-Colbrie R,Winkler H

更新日期：1995-03-06 00:00:00

abstract：:Protein deglutathionylation is mainly catalyzed by glutaredoxins (GRXs). We have analyzed the biochemical properties of four of the six different GRXs of Chlamydomonas reinhardtii. Kinetic parameters were determined for disulfide and dehydroascorbate reduction but also for deglutathionylation of artificial and protein...

journal_title：FEBS letters

authors： Gao XH,Zaffagnini M,Bedhomme M,Michelet L,Cassier-Chauvat C,Decottignies P,Lemaire SD

更新日期：2010-06-03 00:00:00

abstract：:Ghrelin inhibits insulin secretion partly via induction of IA-2beta. However, the orexigenic effect of ghrelin is mediated by the AMP-activated protein kinase (AMPK)-uncoupling protein 2 (UCP2) pathway. Here, we demonstrate that ghrelin's inhibitory effect on insulin secretion also occurs through the AMPK-UCP2 pathway...

journal_title：FEBS letters

authors： Wang Y,Nishi M,Doi A,Shono T,Furukawa Y,Shimada T,Furuta H,Sasaki H,Nanjo K

更新日期：2010-04-16 00:00:00

abstract：:Chimeric antibodies composed of mouse-derived variable regions and human-derived constant regions have been developed for clinical use. However, construction of chimeric mouse/human genes in expression vectors is time-consuming work. In this study, we developed convenient vectors for construction of chimeric mouse/hum...

journal_title：FEBS letters

authors： Kameyama K,Imai K,Itoh T,Taniguchi M,Miura K,Kurosawa Y

更新日期：1989-02-27 00:00:00

abstract：:The 5'-untranslated region (5'-UTR) sequences of 33 GB virus C/hepatitis G virus (GBV-C/HGV) obtained from different geographic areas were determined through reverse-transcription polymerase chain reaction and dideoxy chain termination sequencing, the alignment of sequences, the estimation of the number of nucleotide ...

journal_title：FEBS letters

authors： Mukaide M,Mizokami M,Orito E,Ohba K,Nakano T,Ueda R,Hikiji K,Iino S,Shapiro S,Lahat N,Park YM,Kim BS,Oyunsuren T,Rezieg M,Al-Ahdal MN,Lau JY

更新日期：1997-04-21 00:00:00

abstract：:Two-photon scanning laser and confocal microscopies were used to image metabolic dynamics of single or cell populations of Saccharomyces cerevisiae strain 28033. Autofluorescence of reduced nicotinamide nucleotides, and mitochondrial membrane potential (DeltaPsim), were simultaneously monitored. Spontaneous, large-sca...

journal_title：FEBS letters

authors： Aon MA,Cortassa S,Lemar KM,Hayes AJ,Lloyd D

更新日期：2007-01-09 00:00:00

abstract：:Interaction of the water oxidising manganese complex of photosystem II with the aqueous environment has been investigated using electron paramagnetic resonance spectroscopy and electron spin echo envelope modulation spectroscopy to detect interaction of [2H]methanol with the complex in the S2 state. The experiments sh...

journal_title：FEBS letters

authors： Evans MC,Gourovskaya K,Nugent JH

更新日期：1999-05-07 00:00:00

abstract：:Gene array profiling of RNA from cells engineered to express a dominant-negative version of the ETS family member transcription factor Pea3 (polyomavirus enhancer activator 3) identified WT1 as a candidate downstream gene. Given the co-expression of WT1 and Pea3 in developing kidney and breast tissue undergoing mesenc...

journal_title：FEBS letters

authors： Discenza MT,Vaz D,Hassell JA,Pelletier J

更新日期：2004-02-27 00:00:00

abstract：:HSP73, the constitutive form of heat-shock protein 70, has been implicated in the translocation of preproteins across the mitochondrial membranes, being required for maintaining mitochondrial preproteins in an import competent conformation. Here we report that highly purified mitochondrial outer membranes contain a pr...

journal_title：FEBS letters

authors： Lithgow T,Ryan M,Anderson RL,Høj PB,Hoogenraad NJ

更新日期：1993-10-18 00:00:00

abstract：:Phosphatidylinositol transfer proteins (PITP) are abundant cytosolic proteins originally identified because of their ability to act in vitro as specific transporters of phosphatidylinositol or phosphatidylcholine between membranes. However, the cellular function of mammalian PITP has remained enigmatic till recently. ...

journal_title：FEBS letters

authors： Cockcroft S

更新日期：1997-06-23 00:00:00