Processing of secretogranin II by prohormone convertases: importance of PC1 in generation of secretoneurin.

abstract：:Incorporation of dioleoyl N-(monomethoxy polyethyleneglycol succinyl)phosphatidylethanolamine (PEG-PE) into large unilamellar liposomes composed of egg phosphatidylcholine:cholesterol (1:1) does not significantly increase the content leakage when the liposomes are exposed to 90% human serum at 37 degrees C, yet the li...

journal_title：FEBS letters

authors： Klibanov AL,Maruyama K,Torchilin VP,Huang L

更新日期：1990-07-30 00:00:00

abstract：:Escherichia coli evolves H2 via multiple [Ni-Fe]-hydrogenases (Hyd). This activity under hyper- and hypo-osmotic stress was investigated with mutants lacking different Hyd enzymes during glycerol fermentation. Inhibitory effects of hypo-stress on H2 production was stronger at pH 6.5 in wild type and mutants except fhl...

journal_title：FEBS letters

authors： Trchounian K,Trchounian A

更新日期：2013-11-01 00:00:00

abstract：:The long form of human uncoupling protein-3 (hUCP3L) is highly homologous to thermogenin (UCPI), the uncoupling protein of brown fat mitochondria, but is expressed predominantly in skeletal muscle. Its putative role is to regulate the coupling efficiency of oxidative phosphorylation and thus thermogenesis in skeletal ...

journal_title：FEBS letters

authors： Hinz W,Faller B,Grüninger S,Gazzotti P,Chiesi M

更新日期：1999-04-01 00:00:00

abstract：:A cDNA was cloned from a rabbit spleen cDNA library which encoded a G-protein alpha subunit peptide of 374 amino acids, that at the peptide level exhibited 86% and 79% identity with human Galpha16 and mouse G(alpha)15, respectively. The rabbit G(alpha)subunit cDNA was subcloned into a mammalian expression vector and t...

journal_title：FEBS letters

authors： Feild JA,Foley JJ,Testa TT,Nuthulaganti P,Ellis C,Sarau HM,Ames RS

更新日期：1999-10-22 00:00:00

abstract：:Regulators of G protein signaling RGS4 and RGS7 accelerate the kinetics of K(+) channels (GIRKs) in the Xenopus oocyte system. Here, via quantitative analysis of RGS expression, we reveal biphasic effects of RGSs on GIRK regulation. At low concentrations, RGS4 inhibited basal GIRK activity, but stimulated it at high c...

journal_title：FEBS letters

authors： Keren-Raifman T,Bera AK,Zveig D,Peleg S,Witherow DS,Slepak VZ,Dascal N

更新日期：2001-03-09 00:00:00

abstract：:Trypsin treatment of HeLa cells results in a limited proteolysis of the coxsackievirus and adenovirus receptor (CAR) after which the cleaved CAR remains cell-associated and tryptic peptides remain associated through disulfide bonds. Trypsin-treated HeLa cells remain susceptible to infection with coxsackievirus B and p...

journal_title：FEBS letters

authors： Carson SD

更新日期：2000-11-03 00:00:00

abstract：:Human prothymosin alpha mutants were generated with the aid of random mutagenesis and screened for their ability to inhibit yeast Saccharomyces cerevisiae cell growth. Conversion of Lys-87 to Glu resulted in an inactivated prothymosin alpha mutant, which lost the ability of the wild-type protein to block yeast cell gr...

journal_title：FEBS letters

authors： Rubtsov Y,Vartapetian A

更新日期：1996-11-18 00:00:00

abstract：:The process of cell reprogramming has been characterized considerably since the successful generation of induced pluripotent stem cells. However, the importance of cell-cell communications for cellular reprogramming remains largely unknown. Secreted factors, which are expressed and secreted during reprogramming, may i...

journal_title：FEBS letters

authors： Bansho Y,Lee J,Nishida E,Nakajima-Koyama M

更新日期：2017-06-01 00:00:00

abstract：:To characterize the proteins involved in cell clump/cell adhesion of insect cellular defense reactions, we induced the cell clump/cell adhesion reaction in vitro with the hemolymph of larvae of the coleopteran insect, Tenebrio molitor. The 72 kDa protein was specifically enriched in the residues of cell clump/cell adh...

journal_title：FEBS letters

authors： Lee HS,Cho MY,Lee KM,Kwon TH,Homma K,Natori S,Lee BL

更新日期：1999-02-12 00:00:00

abstract：:Fructose-1,6-bisphosphatase, a key enzyme in gluconeogenesis, undergoes catabolite inactivation when glucose is added to gluconeogenetically active cells of the yeast Saccharomyces cerevisiae. Phosphorylation of the enzyme is followed by rapid degradation. To elucidate the cellular proteolytic system involved in catab...

journal_title：FEBS letters

authors： Schork SM,Bee G,Thumm M,Wolf DH

更新日期：1994-08-01 00:00:00

abstract：:A new mutant alpha-1-antichymotrypsin (variant ACT) was found by direct sequencing and PCR-single strand conformation polymorphism (PCR-SSCP). This variant ACT was a point mutation of exon V of ACT, with the substitution of Met by Val. Four out of six individuals with this variant ACT had occlusive-cerebrovascular dis...

journal_title：FEBS letters

authors： Tsuda M,Sei Y,Yamamura M,Yamamoto M,Shinohara Y

更新日期：1992-06-08 00:00:00

abstract：:Recent studies show LDL receptor-related protein 1B, LRP1B as a transducer of extracellular signals. Here, we identify six interacting partners of the LRP1B cytoplasmic region by yeast two-hybrid screen and confirmed their in vivo binding by immunoprecipitation. One of the partners, PICK1 recognizes the C-terminus of ...

journal_title：FEBS letters

authors： Shiroshima T,Oka C,Kawaichi M

更新日期：2009-01-05 00:00:00

abstract：:Blood flukes of the genus Schistosoma currently infect millions of people in tropical and subtropical countries. An enzyme playing a major role in hemoglobin (Hb) degradation by Schistosoma mansoni has been cloned and shown to be highly similar to the human cathepsin D aspartyl proteinase, although presenting a distin...

journal_title：FEBS letters

authors： Silva FP Jr,Ribeiro F,Katz N,Giovanni-De-Simone S

更新日期：2002-03-13 00:00:00

abstract：:Tight couple 70 S ribosomes are converted to loose couple ones on enzymatic binding of phenylalanyl-tRNA. Enzymatic binding at 0 degree C as well as nonenzymatic binding does not lead to any change. Further, no change takes place when the P site is occupied by N-acetylphenylalanyl-tRNA. Loose couple 70 S ribosomes are...

journal_title：FEBS letters

authors： Srivastava S,Burma DP

更新日期：1985-07-01 00:00:00

abstract：:Michaelis-Menten kinetics of glycerol-3-phosphate dehydrogenase activity in proteoliposomes from brown adipose tissue mitochondria with exogenously added phospholipids or cholesterol was measured. It was shown that changes in membrane lipid composition affected the membrane lateral pressure and therefore modulated the...

journal_title：FEBS letters

authors： Amler E,Jasińska R,Drahota Z,Zborowski J

更新日期：1990-10-01 00:00:00

abstract：:Many receptors coupled to inhibitory Go/Gi-type G proteins often also produce stimulatory signals like Ca2+ mobilisation. When expressed in CHO cells the alpha2-adrenoceptor subtypes alpha2A, alpha2B and alpkha2C mobilised Ca2+. These responses were strongly reduced by the P2Y-purinoceptor antagonist suramin. A large ...

journal_title：FEBS letters

authors： Akerman KE,Näsman J,Lund PE,Shariatmadari R,Kukkonen JP

更新日期：1998-07-03 00:00:00

abstract：:Various molecular mechanisms are involved in the efficacy of arsenic trioxide (ATO) against malignant hematologic and some solid tumors. FLICE-like inhibitory protein (FLIP) is an inhibitor of apoptosis mediated by death receptors. In this study, we identified a new link between the down-regulation of cellular FLIP(L)...

journal_title：FEBS letters

authors： Zhang G,Liu J,Zhang Y,Qu J,Xu L,Zheng H,Liu Y,Qu X

更新日期：2012-09-21 00:00:00

abstract：:We have isolated a full-size cDNA coding for cardiac troponin T (cTnT) from a human adult heart library, using a slow skeletal TnT probe. This cDNA detected a 1.2 kb mRNA in fetal and post-natal human heart, the amount of which increased during ontogenic development. Interestingly, a similar transcript was coexpressed...

journal_title：FEBS letters

authors： Mesnard L,Samson F,Espinasse I,Durand J,Neveux JY,Mercadier JJ

更新日期：1993-08-09 00:00:00

abstract：:Open reading frame 67 of Bombyx mori nucleopolyhedrovirus (BmORF67) is a homologue of Autographa californica multiple NPV ORF81. The gene is conserved among all baculoviruses and is thus considered a baculovirus core gene. The transcript of BmORF67 was detected at 18-72 h post-infection (p.i.). Polyclonal antiserum ra...

journal_title：FEBS letters

authors： Chen HQ,Chen KP,Yao Q,Guo ZJ,Wang LL

更新日期：2007-12-22 00:00:00

abstract：:The hirudin-like factor 1 (HLF1) of Hirudo medicinalis belongs to a new class of leech-derived factors. In previous investigations, HLF1 did not exhibit anticoagulatory activities. Here, we describe the analysis of natural and synthetic variants of HLF1 and HLF-Hyb, a yet uncharacterized member of the HLF family. Modi...

journal_title：FEBS letters

authors： Müller C,Lukas P,Böhmert M,Hildebrandt JP

更新日期：2020-03-01 00:00:00

abstract：:Here, we used a reductant, N-acetyl-L-cysteine (NAC), to investigate the redox-sensitive step(s) in the signalling pathway from the tumor necrosis factor (TNF) receptor to nuclear factor kappaB (NF-kappaB). We found that NAC suppressed NF-kappaB activation triggered by TNF or by overexpression of either the TNF recept...

journal_title：FEBS letters

authors： Oka S,Kamata H,Kamata K,Yagisawa H,Hirata H

更新日期：2000-04-28 00:00:00

abstract：:The effect of platelet-activating factor (PAF) on polyphosphoinositide metabolism and 45Ca2+ efflux was examined in a vascular smooth muscle cell line (A7r5). PAF stimulated a rapid but transient production of inositol trisphosphate and inositol bisphosphate which, in the presence of lithium, resulted in an accumulati...

journal_title：FEBS letters

authors： Doyle VM,Creba JA,Rüegg UT

更新日期：1986-03-03 00:00:00

abstract：:The interaction between two codon-anticodon duplexes of the ribosomal A- and P-site-bound tRNAs is the key feature of the proposed model. This interaction prohibits non-canonical base pairing at the first and second positions of the codon and controls base pairing at the third position (wobbling rules ensuing from the...

journal_title：FEBS letters

authors： Lim VI,Venclovas C

更新日期：1992-11-23 00:00:00

abstract：:Cleavage of the envelope glycoprotein precursor gp160 of HIV-1 is a prerequisite for the infectivity of HIV-1, and occurs at least in part before gp160 reaches the cell surface. Kexin/subtilisin-related endopeptidases are proposed enzyme candidates for this intracellular processing. In this study, we reveal the possib...

journal_title：FEBS letters

authors： Okumura Y,Yano M,Murakami M,Mori S,Towatari T,Kido H

更新日期：1999-01-08 00:00:00

abstract：:Many extracellular stimuli, e.g. microbial products, cytokines etc., result in the expression of inducible nitric oxide synthase (iNOS) in macrophages. However, it is not known whether expression of the iNOS gene in response to microbial products is a primary response of macrophages, or is the result of paracrine/auto...

journal_title：FEBS letters

authors： Dahiya Y,Pandey RK,Bhatt KH,Sodhi A

更新日期：2010-10-08 00:00:00

abstract：:G2/M checkpoints ensure the proper timing of cell mitosis. We previously reported that p38 mitogen-activated protein kinase (MAPK) activation is essential for stress-induced G2 arrest in the U-2OS osteosarcoma cell line, but the molecular mechanism was obscure. Here, using the T7 phage display system, we find p38 dire...

journal_title：FEBS letters

authors： Wu W,Zhou H,He F,Xiao Z,Jiang Y,Zhao M

更新日期：2018-12-01 00:00:00

abstract：:Bromodomains present in Brd4 and other chromatin proteins interact with acetylated histones to regulate transcription and cell growth. To study Brd4-chromatin interactions in vivo, histone H4 tail peptides were fused to a synthetic protein transduction domain (PTD) derived from the human immunodeficiency virus Tat and...

journal_title：FEBS letters

authors： Nishiyama A,Mochizuki K,Mueller F,Karpova T,McNally JG,Ozato K

更新日期：2008-04-30 00:00:00

abstract：:We examined the effects of histidine residues that were artificially introduced into complementarity-determining regions of antibodies on antigen-antibody interactions at different pH values. Using a monoclonal antibody specific for hen egg-white lysozyme and three mutant antibodies that contained a histidine residue,...

journal_title：FEBS letters

authors： Ito W,Sakato N,Fujio H,Yutani K,Arata Y,Kurosawa Y

更新日期：1992-08-31 00:00:00

abstract：:Tropomodulin, the P-end (slow-growing end)-capping protein of the actin-tropomyosin filament, and its fragment (C20) of the C-terminal half were studied by synchrotron small-angle X-ray scattering, restoring low-resolution shapes using an ab initio shape-determining procedure. Tropomodulin is elongated (115 A long) an...

journal_title：FEBS letters

authors： Fujisawa T,Kostyukova A,Maéda Y

更新日期：2001-06-01 00:00:00

abstract：:The anomolous protein synthesis, sensitive to cycloheximide, which has been observed in brain mitochondrial and synaptosomal preparations, has been studied. It is concluded that this protein synthesis is due to the presence, as a contaminant in both preparations, of a ribosome-containing particle which contains solubl...

journal_title：FEBS letters

authors： Morgan IG

更新日期：1970-10-16 00:00:00