Abstract:
:Extracellular ATP itself elicited the generation of superoxide (O2-) in guinea pig peritoneal macrophages associated with an increase in cytosolic calcium ([Ca2+]i). The ATP-induced O2- generation was completely inhibited by pretreatment with pertussis toxin (PT) accompanied by the suppression of [Ca2+]i mobilization. Pre-exposure to a small amount of phorbol myristate acetate (PMA) primed the ATP-induced generation of O2- without a change of [Ca2+]i. The results suggest that ATP-induced O2- generation is mediated by [Ca2+]i mobilization and by PT-sensitive G protein.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Nakanishi M,Takihara H,Minoru Y,Yagawa Kdoi
10.1016/0014-5793(91)80451-8subject
Has Abstractpub_date
1991-04-22 00:00:00pages
91-4issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(91)80451-8journal_volume
282pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::An abundant matrix protein was purified under native conditions from adult bovine tendon and identified as cartilage oligomeric matrix protein (COMP) by immunochemical crossreaction, amino acid sequence identity of tryptic peptides derived from both N- and C-terminal regions, and structure revealed by electron microsc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01134-6
更新日期:1994-11-07 00:00:00
abstract::The complete primary structure (967 amino acids) of an intestinal human aminopeptidase N (EC 3.4.11.2) was deduced from the sequence of a cDNA clone. Aminopeptidase N is anchored to the microvillar membrane via an uncleaved signal for membrane insertion. A domain constituting amino acid 250-555 positioned within the c...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80502-7
更新日期:1988-10-10 00:00:00
abstract::Formation of tubulin/Na(+),K(+)-ATPase (NKA) complex in erythrocytes of hypertensive subjects results in a 50% reduction in NKA activity. We demonstrate here that detyrosinated tubulin, which is increased in hypertensive erythrocytes membranes, enhances the inhibitory effect of acetylated tubulin on NKA activity. More...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.12.022
更新日期:2015-01-30 00:00:00
abstract::Contrary to previously published data, we have found that in mammalian skeletal muscles, phosphoglycerate mutase (PGM) is organized in a regular, striated fashion within the sarcomere. In the absence of the enzyme effectors, PGM localizes mainly at the M-line, but under conditions typical for contracting muscle, the e...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.05.004
更新日期:2009-06-18 00:00:00
abstract::The oxidoreductase ERp57 is a component of the major histocompatibility complex (MHC) class I peptide-loading complex. ERp57 can interact directly with MHC class I molecules, however, little is known about which of the cysteine residues within the MHC class I molecule are relevant to this interaction. MHC class I mole...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.04.034
更新日期:2007-05-15 00:00:00
abstract::Rho proteins are down-regulated in vivo by specific GTPase activating proteins (RhoGAP). We have functionally studied three Saccharomyces cerevisiae putative RhoGAP. By first identifying Rho partners with a systematic two-hybrid approach and then using an in vitro assay, we have demonstrated that the Bag7 protein stim...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02906-4
更新日期:2001-10-05 00:00:00
abstract::Monoamine oxidase B (MAO B) is an outer mitochondrial membrane protein that oxidizes arylalkylamine neurotransmitters and has been a valuable drug target for many neurological disorders. The 1.7 angstrom resolution structure of human MAO B shows the enzyme is dimeric with a C-terminal transmembrane helix protruding fr...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/S0014-5793(04)00209-1
更新日期:2004-04-30 00:00:00
abstract::The trans----cis isomerization of Pro 93 was measured during refolding of bovine ribonuclease A. This isomerization is slow (tau = 500 s) under marginally stable folding conditions of 2.0 M GdmCl, pH 6, at 10 degrees C. However, it is strongly accelerated (tau = 100 s) in samples which, prior to isomerization, had bee...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80408-2
更新日期:1986-03-31 00:00:00
abstract::Nucleoside modifications in tRNA anticodons regulate ribosome dynamics during translation elongation and, thereby, fine-tune global protein synthesis rates. The highly conserved eukaryotic Elongator complex conducts specific C5-substitutions in tRNA wobble base uridines. It harbors two copies of each of its six indivi...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1002/1873-3468.12865
更新日期:2018-02-01 00:00:00
abstract::Stimulation of the bicarbonate dehydration reaction in thylakoid suspension under conditions of saturating light at pH 7.6-8.0 was discovered. This effect was inhibited by nigericin or the lipophilic carbonic anhydrase (CA) inhibitor ethoxyzolamide (EZ), but not by the hydrophilic CA inhibitor, acetazolamide. It was s...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01328-4
更新日期:2000-03-31 00:00:00
abstract::The isolation of a brain peptide identical to the intestinal peptide PHI (peptide HI) is described. The peptide was isolated from porcine brain extract using a chemical assay method based on its C-terminal isoleucine amide structure. The complete amino acid sequence of the peptide was found to be: His-Ala-Asp-Gly-Val-...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80617-6
更新日期:1983-03-21 00:00:00
abstract::Initiation of translation in hepatitis C virus (HCV) is dependent on the presence of an internal ribosome entry site (IRES) contained in its 341-nt-long 5'-untranslated region (UTR). This region is very conserved among different isolates and has been used to classify HCV isolates in six different genotypes. These geno...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00715-1
更新日期:1997-07-14 00:00:00
abstract::We have studied the effect of temperature on the contribution of Hsp104 and trehalose to barotolerance using mutants deficient in Hsp104 and trehalose synthesis. When compared with a corresponding wild type strain, mutants of Hsp104 did not show temperature dependent barotolerance when the incubation temperature durin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01141-1
更新日期:1997-10-13 00:00:00
abstract::The influence of poly (ADP-ribose) polymerase (PARP) and poly ADP-ribosylation on DNA synthesis supported by human replicative DNA polymerase (DNA pol) alpha, delta, and epsilon has been examined using the replication system containing poly(dA)4500-oligo(dT)12-18 as the template primer. PARP alone inhibited the pol ac...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01280-6
更新日期:1994-12-19 00:00:00
abstract::tRNAs play a central role in protein translation, acting as the carrier of amino acids. By cloning microRNAs, we unexpectedly obtained some tRNA fragments generated by tRNA cleavage in the anticodon loop. These tRNA fragments are present in many cell lines and different mouse tissues. In addition, various stress condi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.12.043
更新日期:2009-01-22 00:00:00
abstract::Small organic molecules, like Congo red and lacmoid, have been shown to modulate the self-assembly of the amyloid β peptide (Aβ). Here, we show that Aβ forms NMR invisible non-toxic co-aggregates together with lacmoid as well as Congo red. We find that the interaction involves two distinct kinetic processes and at eve...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.09.035
更新日期:2012-11-16 00:00:00
abstract::The fungal metabolite brefeldin A (BFA) is known to disrupt the Golgi apparatus resulting in redistribution of Golgi proteins to the endoplasmic reticulum and inhibition of protein secretion. BFA was found to inhibit protein synthesis in rat glioma C6 cells by up to 70% between 0.1 and 1 microgram/ml. Inhibition was b...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81508-j
更新日期:1992-12-21 00:00:00
abstract::Auxin increases phospholipase A(2) activity within 2min (Paul, R., Holk, A. and Scherer, G.F.E. (1998) Fatty acids and lysophospholipids as potential second messengers in auxin action. Rapid activation of phospholipase A(2) activity by auxin in suspension-cultured parsley and soybean cells. Plant J. 16, 601-611) and t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.07.059
更新日期:2007-09-04 00:00:00
abstract::This study was designed to investigate changes in gene expression associated with stage-specific programmed cell death (PCD) in intersegmental muscles (ISMs) of the moth, Manduca sexta. The technique of differential display reverse transcription PCR was applied to compare mRNA levels before and after the onset of PCD ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.07.079
更新日期:2005-09-12 00:00:00
abstract::Amino acid sequence alignment of the amino-terminal of thrombomodulin and pancreatic stone protein with the hepatic asialoglycoprotein receptor shows that these proteins are homologous. From the known disulfide bridge pattern of other proteins belonging to the same family two disulfide bonds can be predicted. The homo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80700-2
更新日期:1988-04-11 00:00:00
abstract::A 26 kDa peptide has been cleaved from the C-terminus of the S1 heavy chain with formic acid. Cleavage occurs in the '50 kDa' domain probably at the Asp-600-Pro-601 bond. This fragment has been renatured in the presence of the A2 light chain and the 26 kDa(A2) complex shown to interact with actin in an ATP-sensitive m...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80484-3
更新日期:1989-01-02 00:00:00
abstract::The ability of a yeast nuclear protein to be transported into the nucleus of a higher eukaryotic cell was investigated. Mcm1, a transcriptional activator protein from Saccharomyces cerevisiae, was microinjected into the cytoplasm of Xenopus laevis frog oocytes. Mcm1 was imported into the oocyte nucleus indicating that...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80121-a
更新日期:1993-04-26 00:00:00
abstract::Acetylcholinesterase (AChE) is likely to have roles other than the hydrolysis of acetylcholine, e.g., related to developmental processes like neurite outgrowth, differentiation and adhesion. Here, we investigated whether AChE can function as a heterophilic cell adhesion molecule and searched for proteins interacting w...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.08.078
更新日期:2004-10-08 00:00:00
abstract::A novel member of the neurotrophin family, zebrafish neurotrophin-7 (zNT-7), was isolated from the zebrafish Danio rerio. The amino acid sequence of zNT-7 is more closely related to that of fish nerve growth factor (NGF) and neurotrophin-6 (NT-6) than to that of any other neurotrophin. zNT-7 is, however, equally relat...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00192-6
更新日期:1998-03-13 00:00:00
abstract::A drastic inhibition of protein synthesis occurs in HeLa cells treated with human lymphoblastoid interferon and infected with poliovirus. At the time when this inhibition has been established no degradation of 32P-labelled ribosomal RNA can be detected. Isolation of the mRNAs from poliovirus-infected cells plus or min...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80942-9
更新日期:1983-08-22 00:00:00
abstract::The fluorescence intensity and anisotropy decays of the intrinsic tryptophan emission from six Fe/S proteins (ranging from the very simplest ones to enzyme complexes containing one, two or more Trp residues) were measured. All proteins were examined in the reduced and the oxidized state. In either redox state each pro...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00606-7
更新日期:1994-07-18 00:00:00
abstract::The C-termini of p66 and p51 forms of HIV-1 reverse transcriptase have been engineered to contain a Glu-Glu-Phe sequence recognized by a monoclonal antibody to alpha-tubulin, YL1/2. Mutated RTs were purified in a single step using peptide elution from columns of immobilized YL1/2. The known sequence requirements of th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80613-8
更新日期:1991-06-03 00:00:00
abstract::The effects of ADP, ATP, citrate and EDTA on iron-dependent microsomal and liposomal lipid peroxidation, and on 59FeCl3 binding to the lipid membranes were measured. The aim was to test if initiation of lipid peroxidation is a site-specific mechanism requiring bound iron. In the absence of chelator, iron was bound to ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80131-x
更新日期:1987-05-04 00:00:00
abstract::The [Cu(I)-Cu(II)] half-met form of the dinuclear copper site of tyrosinase has been probed by continuous wave electron paramagnetic resonance (EPR) and hyperfine sublevel correlation (HYSCORE) spectroscopy in the presence and absence of inhibitors. In all cases the EPR spectrum is indicative of a d(x(2)-y(2)) ground ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01609-4
更新日期:2000-06-02 00:00:00
abstract::In humans, many responses to hypoxia including angiogenesis and erythropoiesis are mediated by the alpha/beta-heterodimeric transcription factor hypoxia inducible factor (HIF). The stability and/or activity of human HIF-1alpha are modulated by post-translational modifications including prolyl and asparaginyl hydroxyla...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.02.012
更新日期:2006-04-03 00:00:00