In vivo translational efficiency of different hepatitis C virus 5'-UTRs.

abstract：:Protein import into isolated tobacco mitochondria was investigated using mitochondria from leaves harvested at different times of the day and night. Efficient import was only detected with mitochondria isolated from leaves harvested during the dark period of the growth cycle, only low levels of import were detected fr...

journal_title：FEBS letters

authors： Dessi P,Whelan J

更新日期：1997-09-29 00:00:00

abstract：:We report a new method for the detection of ligand-induced receptor internalization by fluorescence ratiometric imaging of pH in endosomes in combination with a recently developed posttranslational labeling system based on the formation of a heterodimeric coiled-coil structure. The N-terminus of the β2-adrenergic rece...

journal_title：FEBS letters

authors： Yano Y,Matsuzaki K

更新日期：2011-07-21 00:00:00

abstract：:We have investigated the transcriptional activity of human cytomegalovirus, herpes thymidine kinase, human chorionic gonadotropin alpha, somatostatin, immunoglobulin kappa chain, alpha crystallin, albumin and interferon-beta promoters in the fission yeast Schizosaccharomyces pombe. Among these, the human cytomegalovir...

journal_title：FEBS letters

authors： Toyama R,Okayama H

更新日期：1990-07-30 00:00:00

abstract：:There are two common forms of NRH-quinone oxidoreductase 2 (NQO2) in the human population resulting from SNP rs1143684. One has phenylalanine at position 47 (NQO2-F47) and the other leucine (NQO2-L47). Using recombinant proteins, we show that these variants have similar steady state kinetic parameters, although NQO2-L...

journal_title：FEBS letters

authors： Megarity CF,Gill JR,Caraher MC,Stratford IJ,Nolan KA,Timson DJ

更新日期：2014-05-02 00:00:00

abstract：:Glutamate uptake by rat hepatocytes in primary monolayer culture was found to be mediated by a Na+-independent and by two Na+-dependent transport systems of high and low affinity. Inhibition studies with cysteate and other model amino acids rules out the participation of the neutral amino acid transport systems A, ASC...

journal_title：FEBS letters

authors： Gebhardt R,Mecke D

更新日期：1983-09-19 00:00:00

abstract：:The relationship between the antioxidant effects of reduced coenzyme Q10 (ubiquinol, UQH2) and vitamin E (alpha-tocopherol) was investigated in beef heart submitochondrial particles in which lipid peroxidation was initiated by incubation with ascorbate + ADP-Fe3+. These effects were examined after extraction of coenzy...

journal_title：FEBS letters

authors： Forsmark P,Aberg F,Norling B,Nordenbrand K,Dallner G,Ernster L

更新日期：1991-07-08 00:00:00

abstract：:Resonance Raman (RR) spectra of the monomeric cyanomethaemoglobin CTT III from insect larvae of Chironomus thummi thummi are shown for the range of 200-550 cm-1. By iron and cyanide isotope exchange a line varying between 307 cm-1 for 57Fe-13C15N and 311 cm-1 for 54Fe-12C14N, has been assigned to the Fe-N epsilon stre...

journal_title：FEBS letters

authors： Kerr EA,Yu NT,Gersonde K

更新日期：1984-12-03 00:00:00

abstract：:We studied the effects of fibroblast growth factor (FGF-10) on alveolar epithelial cell (AEC) Na,K-ATPase regulation. Within 30 min FGF-10 increased Na,K-ATPase activity and alpha1 protein abundance by 2.5-fold at the AEC plasma membrane. Pretreatment of AEC with the mitogen-activated protein kinase (MAPK) inhibitor U...

journal_title：FEBS letters

authors： Upadhyay D,Lecuona E,Comellas A,Kamp DW,Sznajder JI

更新日期：2003-06-19 00:00:00

abstract：:Accumulation of unfolded proteins in the endoplasmic reticulum (ER) causes ER stress. The ER stress sensor inositol requiring enzyme-1beta (IRE1β), which is specifically expressed in intestinal epithelial cells, is thought to be involved in translational repression. However, its mechanism of action is not fully unders...

journal_title：FEBS letters

authors： Nakamura D,Tsuru A,Ikegami K,Imagawa Y,Fujimoto N,Kohno K

更新日期：2011-01-03 00:00:00

abstract：:Sequence comparison based on Hydrophobic Cluster Analysis procedures shows that the extracellular approximately 200 amino acids domains of cytokines receptors belonging to the Cytokine/Growth hormone/Prolactin receptor family and to the Interferon one are organized in two homologous subdomains. Further, comparison of ...

journal_title：FEBS letters

authors： Thoreau E,Petridou B,Kelly PA,Djiane J,Mornon JP

更新日期：1991-04-22 00:00:00

abstract：:Seven peptides from subunit 9 (S9) of the human 26S proteasome were sequenced and this information was used to clone a HeLa cDNA that encodes the 46 kDa subunit. Rabbit polyclonal antisera were made against a ubiquitin fusion protein containing 12 amino acids from S9 and against a full-length S9 expressed in E. coli. ...

journal_title：FEBS letters

authors： Hoffman L,Rechsteiner M

更新日期：1997-03-10 00:00:00

abstract：:Cell stimulation raised the cytosolic free Ca2+ concentration, [Ca(2+]i and induces activation of Na+/H+ exchange which raises the cytosolic pH, pHi. Recent studies have addressed the question whether Na+/H+ exchange plays a role in Ca(2+) influx and, specifically, whether a rise in pHi alone suffices to open Ca(2+) c...

journal_title：FEBS letters

authors： Siffert W,Akkerman JW

更新日期：1989-12-18 00:00:00

abstract：:We have studied the phosphorylation/dephosphorylation of several nuclear proteins in isolated nuclei from etiolated Avena seedlings as a function of red/far-red light. The effect of stimulatory (ADP-ribosylation by cholera toxin) or inhibitory (GDP beta S) conditions for GTP-binding proteins was also studied. Red or f...

journal_title：FEBS letters

authors： Romero LC,Biswal B,Song PS

更新日期：1991-05-06 00:00:00

abstract：:We show that anthocyanins can function as potent inhibitors of the formation of nitrated tyrosine in vitro, and clarified how pelargonidin (Pel), which has a mono-hydroxyl group on the B-ring, can scavenge peroxynitrite (ONOO(-)) by detection of the reaction products. Pel was reacted with ONOO(-), then the reaction mi...

journal_title：FEBS letters

authors： Tsuda T,Kato Y,Osawa T

更新日期：2000-11-10 00:00:00

abstract：:D-Aspartate increases human chorionic gonadotropin-induced testosterone production in purified rat Leydig cells. L-Aspartate, D-,L-glutamate or D-,L-asparagine could not substitute for D-aspartate and this effect was independent of glutamate receptor activation. Testosterone production was enhanced only in cells cultu...

journal_title：FEBS letters

authors： Nagata Y,Homma H,Lee JA,Imai K

更新日期：1999-02-12 00:00:00

abstract：:A new abnormal hemoglobin, Hb Mito [beta 144(HC 1) Lys----Glu], with an amino acid substitution in the 2,3-DPG pocket, was discovered in a Japanese female. This hemoglobin showed increased oxygen affinity, and decreased organic phosphate and Bohr effects, while the Hill constant n was normal. ...

journal_title：FEBS letters

authors： Harano K,Harano T,Ueda S,Ohkushi T,Imai K

更新日期：1985-11-11 00:00:00

abstract：:Vitamin D3, cholecalciferol, induces cell-cell communication via gap junctions in murine fibroblasts (C3H/10T 1/2 cells) at concentrations between 0.01 and 1.0 microM, as assayed by the dye transfer method using Lucifer yellow CH. The extent of induction is similar to that obtained with the positive controls, canthaxa...

journal_title：FEBS letters

authors： Stahl W,Nicolai S,Hanusch M,Sies H

更新日期：1994-09-19 00:00:00

abstract：:Single-pass sequences were obtained from the 5'-ends of a total of 1794 rose petal cDNA clones. Cluster analysis identified 242 groups of sequences and 635 singletons indicating that the database represents a total of 877 genes. Putative functions could be assigned to 1151 of the transcripts. Expression analysis indic...

journal_title：FEBS letters

authors： Channelière S,Rivière S,Scalliet G,Szecsi J,Jullien F,Dolle C,Vergne P,Dumas C,Bendahmane M,Hugueney P,Cock JM

更新日期：2002-03-27 00:00:00

abstract：:Six charged amino acid residues located in the ectodomain of the full-length type I transforming growth factor (TGF)-beta receptor were individually mutated to alanine. Mutation of residues D47, D98, K102 and E104 resulted in functionally impaired receptors as demonstrated by a marked decrease in ligand-dependent sign...

journal_title：FEBS letters

authors： Guimond A,Sulea T,Zwaagstra JC,Ekiel I,O'Connor-McCourt MD

更新日期：2002-02-27 00:00:00

abstract：:Bovine insulin can be split into several fractions by gel filtration on Sephadex G50. These are, in order of decreasing molecular weight, a new unidentified substance, proinsulin, and a large fraction containing several substances. Ultracentrifugal examination of the proinsulin in acetic acid solution (pH 2.3) gave a ...

journal_title：FEBS letters

authors： Zühlke H,Behlke J

更新日期：1968-12-01 00:00:00

abstract：:In Graves' disease (GD) the presence of antibodies to the thyroid stimulating hormone (TSH) receptor leads to stimulation of the thyroid gland. The thyroid stimulating activity of Graves' IgG is normally ascertained by bioassays measuring cAMP production. We have investigated the effect of Graves' IgG on the quantitat...

journal_title：FEBS letters

authors： Kung AW,Collison K,Banga JP,McGregor AM

更新日期：1988-05-09 00:00:00

abstract：:The relationship between the Fc epsilon receptor mediated stimulation of mast cells and the Ca2+ signal it induces were studied using thapsigargin (TG), a blocker of the endoplasmic reticulum Ca2+ pump. TG induced, in mucosal mast cells (RBL-2H3 line), a dose-dependent and an InsP3-independent increase in [Ca2+]i (fro...

journal_title：FEBS letters

authors： Dar O,Pecht I

更新日期：1992-09-28 00:00:00

abstract：:The presence of G-proteins in the eyespot fraction of Chlamydomonas reinhardtii is shown. This fraction is capable of binding (GTP gamma[35S], possesses the GTPase activity and interacts with antibodies raised against a highly conserved peptide of most G-proteins' alpha-subunit. Cross-reaction with a 24-kDa protein is...

journal_title：FEBS letters

authors： Korolkov SN,Garnovskaya MN,Basov AS,Chunaev AS,Dumler IL

更新日期：1990-09-17 00:00:00

abstract：:Tert-butyl hydroperoxide (BHP), hydrogen peroxide and diamide caused a rapid and simultaneous release of glutathione disulfide (GSSG) and K+ in the isolated perfused rat liver. Both BHP-induced effluxes were suppressed by prior depletion of hepatic glutathione, but not by co-infusion of desferrioxamine which prevented...

journal_title：FEBS letters

authors： Masuda Y,Ozaki M,Aoki S

更新日期：1993-11-08 00:00:00

abstract：:Coronin proteins are widely expressed among eukaryotic organisms. Most coronins consist of a WD-repeat domain followed by a C-terminal coiled coil. Dictyostelium discoideum expresses a single short coronin coronin A, which has been implicated in both actin modulation and multicellular differentiation. Whether coronin ...

journal_title：FEBS letters

authors： Fiedler T,Fabrice TN,Studer V,Vinet A,Faltova L,Kammerer RA,Steinmetz MO,Sharpe T,Pieters J

更新日期：2020-04-16 00:00:00

abstract：:We have used DNase I footprinting to examine the effect of a novel naphthylquinoline dimer, designed as a triplex-specific bis-intercalator, on the stability of intermolecular DNA triplexes. We find that this compound efficiently promotes triplex formation between the 9-mer oligonucleotide 5'-TTTTTTCTT and its oligopu...

journal_title：FEBS letters

authors： Keppler M,Zegrocka O,Strekowski L,Fox KR

更新日期：1999-03-26 00:00:00

abstract：:This study was undertaken to demonstrate the unique specificity of the chemokine receptor CXCR4 antagonist AMD3100. Calcium flux assays with selected chemokine/cell combinations, affording distinct chemokine receptor specificities, revealed no interaction of AMD3100 with any of the chemokine receptors CXCR1 through CX...

journal_title：FEBS letters

authors： Hatse S,Princen K,Bridger G,De Clercq E,Schols D

更新日期：2002-09-11 00:00:00

abstract：:Neopterin is synthesized by human monocyte-derived macrophages primarily upon stimulation with the cytokine interferon-gamma. We studied the influence of neopterin on the generation of reactive oxygen species (ROS) in human peripheral blood neutrophils. Radical formation was measured using a biochemiluminometer. Neutr...

journal_title：FEBS letters

authors： Razumovitch JA,Semenkova GN,Fuchs D,Cherenkevich SN

更新日期：2003-08-14 00:00:00

abstract：:Osteoblastic differentiation is regulated by various factors, including hormones and transcription factors. Runt-related transcription factor 2 (Runx2) is an essential player in osteoblastogenesis and transactivates its molecular target by creating a protein complex with its hetero-dimeric partner core binding factor ...

journal_title：FEBS letters

authors： Fukuda T,Ochi H,Sunamura S,Haiden A,Bando W,Inose H,Okawa A,Asou Y,Takeda S

更新日期：2015-10-24 00:00:00

abstract：:We tested the truncated 7-37 glucagon-like peptide 1 (TGLP-1), a naturally occurring porcine intestinal peptide, and other members of the glucagon family, including pancreatic glucagon (G-29), GLP-1 and GLP-2 for their ability to activate the cAMP generating system in rat gastric glands and HGT-1 human gastric cancer ...

journal_title：FEBS letters

authors： Hansen AB,Gespach CP,Rosselin GE,Holst JJ

更新日期：1988-08-15 00:00:00