Structural symmetry of the extracellular domain of the cytokine/growth hormone/prolactin receptor family and interferon receptors revealed by hydrophobic cluster analysis.

abstract：:Increasingly available genomic sequencing data are exploited to identify genes and variants contributing to diseases, particularly cancer. Traditionally, methods to find such variants have relied heavily on allele frequency and/or familial history, often neglecting to consider any mechanistic understanding of their fu...

journal_title：FEBS letters

authors： González-Sánchez JC,Raimondi F,Russell RB

更新日期：2018-02-01 00:00:00

abstract：:Using ultrastructural analysis and labeling with polyclonal antibodies that recognize peptide sequences specific for phospholipid binding, we mapped the functional domain structure of intact platelet talin and its proteolytic fragments. The talin dimer, which is crucial for actin and lipid binding, is built of a backb...

journal_title：FEBS letters

authors： Isenberg G,Goldmann WH

更新日期：1998-04-17 00:00:00

abstract：:Many genes are regulated by the intracellular calcium, protein kinase C (PKC) and protein kinase A (PKA) pathways and it has been shown that these pathways synergize in some cell types, whereas they antagonize in others. Here we show that the calcium and PKC pathways suppress the effects mediated by the PKA pathway in...

journal_title：FEBS letters

authors： Döbbeling U,Berchtold MW

更新日期：1996-08-05 00:00:00

abstract：:Two hexokinases were characterized in Schizosaccharomyces pombe: hexokinase 1, with a low phosphorylation coefficient on glucose (Km 8.5 mM) and hexokinase 2, a kinetically conventional hexokinase. Genes hxk1+ and hxk2+ encoding these enzymes were cloned and sequenced. Disruption of hxk1+ had no effect on growth but d...

journal_title：FEBS letters

authors： Petit T,Blázquez MA,Gancedo C

更新日期：1996-01-08 00:00:00

abstract：:We present an open-access web platform isomiRex, to identify isomiRs and on the fly graphical visualization of the differentially expressed miRNAs in control as well as treated library. The open-access web-platform is not restricted only to NGS sequence dataset from animals and potentially analyzes a wider dataset for...

journal_title：FEBS letters

authors： Sablok G,Milev I,Minkov G,Minkov I,Varotto C,Yahubyan G,Baev V

更新日期：2013-08-19 00:00:00

abstract：:We have expressed green fluorescent protein (GFP) from A. victoria in the secretory pathway of HeLa cells by fusing it to the C-terminus of a secretory protein, chromogranin B. Under normal culture conditions at 37 degrees C maturation of GFP to the fluorescent form was not detectable. However, fluorescent GFP was obs...

journal_title：FEBS letters

authors： Kaether C,Gerdes HH

更新日期：1995-08-07 00:00:00

abstract：:We have characterized the glucose transport system in the Kc cells of Drosophila melanogaster. The transporter is stereospecific and can be inhibited by both cytochalasin B and phloretin, with ki values of 4 microM and 30 microM for cytochalasin B and phloretin, respectively. Moreover, phlordzin does not affect uptake...

journal_title：FEBS letters

authors： Wang M,Wang C

更新日期：1993-02-15 00:00:00

abstract：:A large number of protein-protein interactions involved turn or loop regions. The excised linear peptides from these regions reveal complex conformational averaging. To circumvent this motional averaging and to stabilize the beta-turn conformation, extensive effort has been devoted to the design of constrained peptido...

journal_title：FEBS letters

authors： Lee MS,Gardner B,Kahn M,Nakanishi H

更新日期：1995-02-13 00:00:00

abstract：:Both acute myeloid leukemia 1 and c-Fos are regulatory factors of hematopoietic cell differentiation. We identified that the c-fos promoter contains an acute myeloid leukemia 1 binding site at nucleotide positions -6-+14. c-fos promoter activity was induced by transient overexpression of acute myeloid leukemia 1 in Ju...

journal_title：FEBS letters

authors： Hwang ES,Hong JH,Bae SC,Ito Y,Lee SK

更新日期：1999-03-05 00:00:00

abstract：:The PDE4 catalytic machinery comprises, in part, two divalent cations in a binuclear motif. Here we report that PDE4A4 expressed in Sf9 cells exhibits a biphasic Mg(2+) dose-response (EC(50) of 0.15 and >10 mM) in catalyzing cAMP hydrolysis. In vitro phosphorylation of PDE4A4 by the PKA-catalytic subunit increases the...

journal_title：FEBS letters

authors： Laliberté F,Liu S,Gorseth E,Bobechko B,Bartlett A,Lario P,Gresser MJ,Huang Z

更新日期：2002-02-13 00:00:00

abstract：:We have measured SGLT1 mRNA content and SGLT1-mediated glucose transport at different positions along the small intestine of control and streptozotocin diabetic rats and shown both parameters to be similar but higher in jejunal compared with ileal tissue. No such correlation was seen when comparing measurements of SGL...

journal_title：FEBS letters

authors： Dong R,Srai SK,Debnam E,Smith M

更新日期：1997-04-07 00:00:00

abstract：:The control of physiological processes requires the regulation and coordination of many different signals and is determined in part by the activation and repression of expression of specific target genes. RIP140 is a ligand dependent coregulator of many nuclear receptors that influence such diverse processes as muscle...

journal_title：FEBS letters

authors： White R,Morganstein D,Christian M,Seth A,Herzog B,Parker MG

更新日期：2008-01-09 00:00:00

abstract：:Rho proteins and phosphatidylinositide 3-kinases (PI 3-kinases) have been widely implicated in regulating cell motility both in cultured cells and in animal models. Monocytes are recruited from the bloodstream in response to inflammatory signals, and migrate across the endothelial barrier into the tissues, where they ...

journal_title：FEBS letters

authors： Ridley AJ

更新日期：2001-06-08 00:00:00

abstract：:IRF-8/ICSBP and IRF-1 are IRF family members whose expression is induced in response to IFN-gamma in macrophages. IL-12 is a cytokine produced in macrophages that plays a critical role in host defense. IFN-gamma and bacterial lipopolysaccharide (LPS) induce IL-12p40 transcription, which is necessary for the production...

journal_title：FEBS letters

authors： Masumi A,Tamaoki S,Wang IM,Ozato K,Komuro K

更新日期：2002-11-06 00:00:00

abstract：:An alpha beta heterodimer of the F1-ATPase of Rhodospirillum rubrum was isolated by extraction of chromatophores with LiCl. Each alpha beta heterodimer contains one tightly bound ADP, which is released upon removal of medium Mg2+. The dimer can be reversibly dissociated by removal of Mg(2+)-ions. The alpha beta hetero...

journal_title：FEBS letters

authors： Andralojc PJ,Harris DA

更新日期：1992-09-28 00:00:00

abstract：:'Peripheral' binding sites for benzodiazepines are under neural or homonal control in the pineal gland, olfactory bulb, and kidney. These observations prompted a search for an endogenous substance which could modulate these sites under physiological conditions. Acidified methanol extracts from several tissues (e.g. st...

journal_title：FEBS letters

authors： Mantione CR,Weissman BA,Goldman ME,Paul SM,Skolnick P

更新日期：1984-10-15 00:00:00

abstract：:The inhibition of electron transport by unnatural (-)-antimycin A3 was examined with rat liver mitochondria and compared with that of natural (+)-antimycin A3. (-)-Antimycin A3 inhibited respiration about 1/100th as strongly as natural (+)-antimycin A3. (-)-Antimycin A3 binding to the cytochrome bc1 complex did not se...

journal_title：FEBS letters

authors： Miyoshi H,Kondo H,Oritani T,Saitoh I,Iwamura H

更新日期：1991-11-04 00:00:00

abstract：:A human vesicular monoamine transporter cDNA has been identified by screening a human brainstem library using sequences from the rat brain synaptic vesicle monoamine transporter (SVMT) [(1992) Cell 70, 539-551; (1992) Proc. Natl. Acad. Sci. USA 89, 10993-10997]. The hSVMT shares 92% amino acid identity with the rat se...

journal_title：FEBS letters

authors： Surratt CK,Persico AM,Yang XD,Edgar SR,Bird GS,Hawkins AL,Griffin CA,Li X,Jabs EW,Uhl GR

更新日期：1993-03-08 00:00:00

abstract：:To evaluate ozone damage to hairless mouse skin, two parameters of oxidative damage, vitamin E depletion and malondialdehyde (MDA) production, were measured in vitamin E-enriched and in control skin from mice exposed to ozone (10 ppm). A 5% vitamin E solution (tocotrienol-rich fraction, TRF) in polyethylene glycol (PE...

journal_title：FEBS letters

authors： Thiele JJ,Traber MG,Podda M,Tsang K,Cross CE,Packer L

更新日期：1997-01-20 00:00:00

abstract：:DNA polymerase (pol) λ, one of the 15 cellular pols, belongs to the X family. It is a small 575 amino-acid protein containing a polymerase, a dRP-lyase, a proline/serine rich and a BRCT domain. Pol λ shows various enzymatic activities including DNA polymerization, terminal transferase and dRP-lyase. It has been implic...

journal_title：FEBS letters

authors： Markkanen E,van Loon B,Ferrari E,Hübscher U

更新日期：2011-09-16 00:00:00

abstract：:Light and heavy lysosomes of mouse forebrain were separated from each other by centrifugation on a Percoll gradient. Light lysosomes were then freed from mitochondria and membranes by sucrose density gradient centrifugation and further purified by floatation-centrifugation on a sucrose gradient. The final preparations...

journal_title：FEBS letters

authors： Fiorilli A,Siniscalco C,Chiarini A,Di Francesco L,Venerando B,Tettamanti G

更新日期：1991-05-06 00:00:00

abstract：:Although neurokinin A (NKA), a tachykinin peptide with sequence homology to substance P (SP), is a weak competitor of radiolabeled SP binding to the NK-1 receptor (NK-1R), more recent direct binding studies using radiolabeled NKA have demonstrated an unexpected high-affinity interaction with this receptor. To document...

journal_title：FEBS letters

authors： Bremer AA,Leeman SE,Boyd ND

更新日期：2000-12-01 00:00:00

abstract：:Strigolactones are a new class of phytohormones synthesized from carotenoids via carlactone. The complex structure of carlactone is not easily deducible from its precursor, a cis-configured β-carotene cleavage product, and is thus formed via a poorly understood series of reactions and molecular rearrangements, all cat...

journal_title：FEBS letters

authors： Bruno M,Vermathen M,Alder A,Wüst F,Schaub P,van der Steen R,Beyer P,Ghisla S,Al-Babili S

更新日期：2017-03-01 00:00:00

abstract：:The Surfeit locus is an unusual cluster of at least 6 housekeeping genes whose organisation is conserved between birds and mammals. We have previously shown that the divergently transcribed Surf-1 and Surf-2 genes are separated by a bi-directional, TATA-less promoter. In mouse, the Surf-1/Surf-2 promoter contains thre...

journal_title：FEBS letters

authors： Gaston K,Fried M

更新日期：1994-06-27 00:00:00

abstract：:Maintaining genomic integrity is vital for cell survival and homeostasis. Mutations in critical genes in germ-line and somatic cells are often implicated with the onset or progression of diseases. DNA repair enzymes thus take important roles as guardians of the genome in the cell. Besides the known function to repair ...

journal_title：FEBS letters

authors： Bunch H

更新日期：2016-04-01 00:00:00

abstract：:The promoter of the cholecystokinin (CCK) gene possesses evolutionary conserved juxtaposed E-box and cAMP/TPA responsive elements (CRE/TRE). We have examined the functional interaction of these two sites. As previously noted, c-Jun/c-Fos heterodimers greatly increase promoter activity through association with the CRE/...

journal_title：FEBS letters

authors： Rourke IJ,Hansen TV,Nerlov C,Rehfeld JF,Nielsen FC

更新日期：1999-04-01 00:00:00

abstract：:The sarcoplasmic reticulum Ca(2+)-ATPase loses hydrolytic activity and the ability to be phosphorylated by Pi following incubation with EDC [1-ethyl-3-(3-dimethylaminopropyl)carbodiimide]. 4 nmol of tempamine per mg SR protein can be coupled to either a glu or an asp side chain through the EDC reaction. Mg2+ protects ...

journal_title：FEBS letters

authors： Coan C,Jakobs P,Ji JY,Murphy AJ

更新日期：1993-11-29 00:00:00

abstract：:The uncoupler-induced inactivation of H+-ATPase in hepatoma 22a and mouse liver mitochondria has been studied. The dependence of this process on delta microH, and pH and ATP was established. The inactivated ATPase could be reactivated at alkaline pH values in the absence of ATP. These data indicate that the inactivati...

journal_title：FEBS letters

authors： Chernyak BV,Dukhovich VF,Khodjaev EYu

更新日期：1987-05-11 00:00:00

abstract：:Dendritic cells (DC) were enriched from murine spleen by exploring their intermediate density and transient weak adherence. The isolated population contained excellent antigen presenting cells with high surface expression of major histocompatibility complex (MHC) class II determinants thus exhibiting crucial immunofun...

journal_title：FEBS letters

authors： Fischer HG,Eder C

更新日期：1995-10-09 00:00:00

abstract：:Spermidine N1-acetyltransferase in an extract from phytohemagglutinin-stimulated bovine lymphocytes was inactivated by preincubation with alkaline phosphatase. Inactivation of the acetylase with the phosphatase was totally inhibited by addition of pyrophosphate. These results suggest that spermidine N1-acetyltransfera...

journal_title：FEBS letters

authors： Matsui I,Otani S,Kamei M,Morisawa S

更新日期：1982-12-13 00:00:00