Abstract:
:Many genes are regulated by the intracellular calcium, protein kinase C (PKC) and protein kinase A (PKA) pathways and it has been shown that these pathways synergize in some cell types, whereas they antagonize in others. Here we show that the calcium and PKC pathways suppress the effects mediated by the PKA pathway in a fibroblast cell line. The suppressing effect of elevated intracellular Ca2+ levels, but not of the PKC pathway, can be abrogated by the addition of cyclosporin A (CsA), indicating that the effect of Ca2+ is mediated by phosphatase-2B (PP-2B/calcineurin). Suppression by the PKC pathway is not mediated by the proto-oncogenes c-fos, c-jun and junB, as the co-transfection of these genes does not block the effects of the PKA stimulator 8-Br-cAMP. In addition, cotransfection with the catalytic subunit of PKA shows that the inhibitory effect of PKC occurs upstream of PKA activation.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Döbbeling U,Berchtold MWdoi
10.1016/0014-5793(96)00748-xsubject
Has Abstractpub_date
1996-08-05 00:00:00pages
131-3issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(96)00748-Xjournal_volume
391pub_type
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