Functional relevance of the internal hydrophobic cavity of urate oxidase.

Abstract:

:Urate oxidase from Aspergillus flavus is a 135 kDa homo-tetramer which has a hydrophobic cavity buried within each monomer and located close to its active site. Crystallographic studies under moderate gas pressure and high hydrostatic pressure have shown that both gas presence and high pressure would rigidify the cavity leading to an inhibition of the catalytic activity. Analysis of the cavity volume variations and functional modifications suggest that the flexibility of the cavity would be an essential parameter for the active site efficiency. This cavity would act as a connecting vessel to give flexibility to the neighboring active site, and its expansion under pure oxygen pressure reveals that it might serve as a transient reservoir on its pathway to the active site.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Colloc'h N,Prangé T

doi

10.1016/j.febslet.2014.03.017

subject

Has Abstract

pub_date

2014-05-02 00:00:00

pages

1715-9

issue

9

eissn

0014-5793

issn

1873-3468

pii

S0014-5793(14)00222-1

journal_volume

588

pub_type

杂志文章
  • Mechanism of o-aminophenol metabolism in human erythrocytes.

    abstract::o-Aminophenol was found to be rapidly metabolized to a brown compound in the presence of purified human oxy- and methemoglobin, coupled with the oxidation and reduction of these hemoglobins by o-aminophenol. The final product of o-aminophenol was identified as 2-aminophenoxazine-3-one, by using spectrophotometry and H...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(86)80211-3

    authors: Tomoda A,Yamaguchi J,Kojima H,Amemiya H,Yoneyama Y

    更新日期:1986-02-03 00:00:00

  • The molecular peculiarities of catalase-peroxidases.

    abstract::In developing ideas of how protein structure modifies haem reactivity, the activity of Class I of the plant peroxidase superfamily (including cytochrome c peroxidase, ascorbate peroxidase and catalase-peroxidases (KatGs)) is an exciting field of research. Despite striking sequence homologies, there are dramatic differ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(01)02237-2

    authors: Zámocký M,Regelsberger G,Jakopitsch C,Obinger C

    更新日期:2001-03-16 00:00:00

  • Sodium butyrate suppresses apoptosis in human Burkitt lymphomas and murine plasmacytomas bearing c-myc translocations.

    abstract::We report that sodium butyrate, a natural product of fiber degradation by colonic bacteria, markedly suppresses c-Myc-mediated apoptosis induced in murine plasmacytomas and human Burkitt lymphomas by growth factor deprivation, but not in cell lines devoid of c-myc translocations. Attenuation of cell death is associate...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(98)00926-0

    authors: Alexandrov I,Romanova L,Mushinski F,Nordan R

    更新日期:1998-08-28 00:00:00

  • Mis-annotations of a promising antibiotic target in high-priority gram-negative pathogens.

    abstract::The rise of antibiotic resistance combined with the lack of new products entering the market has led to bacterial infections becoming one of the biggest threats to global health. Therefore, there is an urgent need to identify novel antibiotic targets, such as dihydrodipicolinate synthase (DHDPS), an enzyme involved in...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1002/1873-3468.13733

    authors: Impey RE,Lee M,Hawkins DA,Sutton JM,Panjikar S,Perugini MA,Soares da Costa TP

    更新日期:2020-05-01 00:00:00

  • WWamide-1, -2 and -3: novel neuromodulatory peptides isolated from ganglia of the African giant snail, Achatina fulica.

    abstract::Three novel neuropeptides, isolated from ganglia of the African giant snail, Achatina fulica, were named WWamide-1, -2 and -3. These substances were biologically active heptapeptide amides with a Trp residue at both the N- and C-termini. WWamide-1, which displayed an inhibitory activity on a central neuron of the snai...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(93)81458-c

    authors: Minakata H,Ikeda T,Muneoka Y,Kobayashi M,Nomoto K

    更新日期:1993-05-24 00:00:00

  • Geometry of binding of the N alpha-tosylated piperidides of m-amidino-, p-amidino- and p-guanidino phenylalanine to thrombin and trypsin. X-ray crystal structures of their trypsin complexes and modeling of their thrombin complexes.

    abstract::The X-ray crystal structures of the complexes formed with bovine trypsin and the N alpha-tosylated piperidides of m-amidino-, p-amidino- and p-guanidino-D,L-phenylalanine (3-TAPAP, 4-TAPAP and 4-TGPAP) were determined with data to 1.8 A resolution. The L-stereoisomer of 3-TAPAP binds as a compact entity into the activ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(91)80033-y

    authors: Turk D,Stürzebecher J,Bode W

    更新日期:1991-08-05 00:00:00

  • Folding kinetics of the lipoic acid-bearing domain of human mitochondrial branched chain alpha-ketoacid dehydrogenase complex.

    abstract::A reversible two-step (native state<-->denatured state) folding mechanism based on equilibrium and stopped flow experiments is proposed for urea denaturation of the lipoyl-bearing domain (hbLBD) of human mitochondrial branched chain alpha-ketoacid dehydrogenase (BCKD) complex. The results from this circular dichroism ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(02)03444-0

    authors: Naik MT,Chang YC,Huang TH

    更新日期:2002-10-23 00:00:00

  • Phenylalanine ammonia-lyase immobilized in semipermeable microcapsules for enzyme replacement in phenylketonuria.

    abstract::Phenylalanine ammonia-lyase immobilized within semipermeable microcapsules has an assayed enzyme activity which is 20% +/- 4% of the enzyme in free solution. The Km for the immobilized enzyme remained the same as that of the free enzyme. The pH optimum also remained unchanged at pH 8.5 +/- 1.0. At the lower pH range, ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(85)80219-2

    authors: Bourget L,Chang TM

    更新日期:1985-01-21 00:00:00

  • Evidence for a role of C-terminus in Ca(2+) inactivation of skeletal muscle Ca(2+) release channel (ryanodine receptor).

    abstract::Six chimeras of the skeletal muscle (RyR1) and cardiac muscle (RyR2) Ca(2+) release channels (ryanodine receptors) previously used to identify RyR1 dihydropyridine receptor interactions [Nakai et al. (1998) J. Biol. Chem. 273, 13403] were expressed in HEK293 cells to assess their Ca(2+) dependence in [(3)H]ryanodine b...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(99)01232-6

    authors: Nakai J,Gao L,Xu L,Xin C,Pasek DA,Meissner G

    更新日期:1999-10-08 00:00:00

  • Utilization of alanine for glucose formation in isolated rabbit kidney-cortex tubules.

    abstract::In kidney cortex tubules isolated from fed rabbits L-alanine is not utilized as glucose precursor, when added as a sole substrate. However, this amino acid decreases gluconeogenesis from low (up to 1 mM) 2-oxoglutarate concentrations and stimulates this process at higher (2.5-10 mM) ketoacid contents in the suspension...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(89)81514-5

    authors: Zabłocki K,Bryła J

    更新日期:1989-12-18 00:00:00

  • A 28 kDa mitochondrial protein is radiolabelled by crosslinking with a 125I-labelled presequence.

    abstract::A 13-residue peptide containing the first 12 amino acids of the N-terminal part of the signal sequence of yeast cytochrome c oxidase subunit IV is shown by chemical crosslinking to interact with a mitochondrial protein. This result is obtained with mitochondria from four different origins. Submitochondrial localizatio...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(91)80262-2

    authors: Font B,Goldschmidt D,Chich JF,Thieffry M,Henry JP,Gautheron DC

    更新日期:1991-02-11 00:00:00

  • Identification of novel cellular proteins that bind to the LC8 dynein light chain using a pepscan technique.

    abstract::Dynein is a minus end-directed microtubule motor that serves multiple cellular functions. We have performed a fine mapping of the 8 kDa dynein light chain (LC8) binding sites throughout the development of a library of consecutive synthetic dodecapeptides covering the amino acid sequences of the various proteins known ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(01)02718-1

    authors: Rodríguez-Crespo I,Yélamos B,Roncal F,Albar JP,Ortiz de Montellano PR,Gavilanes F

    更新日期:2001-08-17 00:00:00

  • Modulation of gastric H+,K+-transporting ATPase function by sodium.

    abstract::Gastric H+,K+-ATPase activity is not affected by Na+ at pH 7.0 but is significantly stimulated by Na+ at pH 8.5. For the stimulation at the latter pH, the presence of both Na+ and K+ were essential. Contrary the H+,K+-ATPase, the associated K+-pNPPase was inhibited by Na+ at both pH values. Sodium competes with K+ for...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(85)80733-x

    authors: Ray TK,Nandi J

    更新日期:1985-06-03 00:00:00

  • Characterisation of cyclin D1 down-regulation in coronavirus infected cells.

    abstract::The positive strand RNA coronavirus, infectious bronchitis virus (IBV), induces a G2/M phase arrest and reduction in the G1 and G1/S phase transition regulator cyclin D1. Quantitative real-time RT-PCR and Western blot analysis demonstrated that cyclin D1 was reduced post-transcriptionally within infected cells indepen...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2007.02.039

    authors: Harrison SM,Dove BK,Rothwell L,Kaiser P,Tarpey I,Brooks G,Hiscox JA

    更新日期:2007-04-03 00:00:00

  • Constitutively active Cullin-RING-Ligases fail to rescue loss of NEDD8 conjugation in Schizosaccharomyces pombe.

    abstract::In fission yeast, the only known essential function of Ned8p is the modification of the cullin, Pcu1p, and subsequent Cullin-RING-Ligase (CRL) activation and substrate ubiquitination. We show here that a functional Pcu1p mutant, deleted for its C-terminal autoinhibitory domain, which negates the requirement of neddyla...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2012.04.011

    authors: Girdwood D,Robertson M,Gordon C

    更新日期:2012-05-21 00:00:00

  • Histological mapping of biochemical changes in solid tumors by FT-IR spectral imaging.

    abstract::Fourier-transform infrared (FT-IR) spectral imaging was used for analyzing biochemical changes in tumor cells. Metabolic parameters of human lung A549/8 adenocarcinoma and U87 glioma cells were compared under stress conditions in culture along with tumor progression after cell implantation onto the chick embryo chorio...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2007.10.052

    authors: Petibois C,Drogat B,Bikfalvi A,Déléris G,Moenner M

    更新日期:2007-11-27 00:00:00

  • Structure of DNA in metaphase chromosomes of mouse fibroblasts.

    abstract::We show that N-1 in adenine of chromosomal DNA is methylated by treatment of metaphase chromosomes with dimethylsulphate while this is not the case in chromatin. The data on methylation are consistent with those obtained from the experiments with S1-nuclease treatment of chromatin and chromosomes. This suggests a disa...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(83)80167-7

    authors: Belyaev ND,Budker VG,Dubrovskaya VA,Matveeva NM

    更新日期:1983-04-18 00:00:00

  • Functional characterization of a NapA Na(+)/H(+) antiporter from Thermus thermophilus.

    abstract::Na(+)/H(+) antiporters are ubiquitous membrane proteins and play an important role in cell homeostasis. We amplified a gene encoding a member of the monovalent cation:proton antiporter-2 (CPA2) family (TC 2.A.37) from the Thermus thermophilus genome and expressed it in Escherichia coli. The gene product was identified...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2006.12.059

    authors: Furrer EM,Ronchetti MF,Verrey F,Pos KM

    更新日期:2007-02-06 00:00:00

  • Lcb4p sphingoid base kinase localizes to the Golgi and late endosomes.

    abstract::Sphingoid long chain base phosphates (LCBPs) regulate cell proliferation, survival and motility in mammals. To learn more about LCBPs in Saccharomyces cerevisiae, we determined the cellular location of Lcb4p, the major enzyme catalyzing LCBP synthesis. By indirect immunofluorescence microscopy and subcellular fraction...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(02)03636-0

    authors: Hait NC,Fujita K,Lester RL,Dickson RC

    更新日期:2002-12-04 00:00:00

  • Change in charge of an unvaried heme contact residue does not cause a major change of conformation in cytochrome c.

    abstract::The structure of the Ala38 variant of yeast iso-1-cytochrome c, in which the previously unchanged Arg38 has been replaced, has been characterised by NMR. The NMR data indicate that the structure of the Ala38 variant is very similar to that of the wild type protein. In particular, the heme environment and interactions ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(91)80678-v

    authors: Thurgood AG,Pielak GJ,Cutler RL,Davies AM,Greenwood C,Mauk AG,Smith M,Williamson DJ,Moore GR

    更新日期:1991-06-24 00:00:00

  • Heme a induces assembly of rat liver cytochrome c oxidase subunits I--III in isolated mitochondria.

    abstract::The assembly of cytochrome c oxidase subunits I-III was studied in vitro in isolated rat liver mitochondria pre-labeled with [35S]methionine. Individual subunits were immunoabsorbed with monospecific antibodies. Isolated heme a from rat liver mitochondria, when added to radiolabeled mitochondria, induced assembly of s...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(84)81302-2

    authors: Wielburski A,Nelson BD

    更新日期:1984-11-19 00:00:00

  • Does the HIV Nef protein mimic the MHC?

    abstract::The sequence of the HIV Nef protein has no significant homology to other proteins in the SwissProt database, and experimental data concerning its function are sparse and contradictory. Using a novel protein sequence comparison method, we find similarities between different Nef sequences and the alpha chain of human MH...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(93)80655-e

    authors: Hobohm U,Sander C

    更新日期:1993-11-01 00:00:00

  • Immunological characterization of microtubule-associated proteins specific for the immature brain.

    abstract::Immunoblotting analysis was used to detect the microtubule-associated proteins present at different stages of rat brain development. Polyclonal antibodies were raised against the two main adult brain microtubule-associated proteins: MAP-2 (300 kDa) and TAU (60-70 kDa). Whatever the stage of development, anti-MAP-2 ser...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(85)80397-5

    authors: Couchie D,Nunez J

    更新日期:1985-09-02 00:00:00

  • Functional characterization of the rat multispecific organic anion transporter OAT1 mediating basolateral uptake of anionic drugs in the kidney.

    abstract::The functional characteristics of rat organic anion transporter OAT1 were investigated using Xenopus laevis oocytes. Uptake of p-aminohippurate (PAH) by the oocytes expressing OAT1 was markedly inhibited by glutarate, alpha-ketoglutarate and probenecid, moderately inhibited by folate and methotrexate, but not inhibite...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(98)01328-3

    authors: Uwai Y,Okuda M,Takami K,Hashimoto Y,Inui K

    更新日期:1998-11-06 00:00:00

  • IκBL, a novel member of the nuclear IκB family, inhibits inflammatory cytokine expression.

    abstract::We previously reported that IκBL prevents experimental autoimmune arthritis. The molecular mechanism, however, still remains unclear. In contrast to four splicing-isoforms of IκBL in human, two isoforms were identified in mouse. The major isoform IκBL-α(S) suppressed LPS-induced NF-κB activation and transcription of T...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2011.10.024

    authors: Chiba T,Miyashita K,Sugoh T,Warita T,Inoko H,Kimura M,Sato T

    更新日期:2011-11-16 00:00:00

  • Purified recombinant hARD1 does not catalyse acetylation of Lys532 of HIF-1alpha fragments in vitro.

    abstract::In humans, many responses to hypoxia including angiogenesis and erythropoiesis are mediated by the alpha/beta-heterodimeric transcription factor hypoxia inducible factor (HIF). The stability and/or activity of human HIF-1alpha are modulated by post-translational modifications including prolyl and asparaginyl hydroxyla...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2006.02.012

    authors: Murray-Rust TA,Oldham NJ,Hewitson KS,Schofield CJ

    更新日期:2006-04-03 00:00:00

  • Involvement of the NF-kappaB pathway in the transforming properties of the TEL-Jak2 leukemogenic fusion protein.

    abstract::Constitutively active tyrosine kinases are frequently expressed in various types of human leukemias as the result of chromosomal translocations. The TEL-Jak2 fusion oncoprotein possesses transforming properties in both animal and cellular models, that are tightly dependent on Stat5 activation. In the IL-3-independent ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(01)02452-8

    authors: Santos SC,Monni R,Bouchaert I,Bernard O,Gisselbrecht S,Gouilleux F,Penard-Lacronique V

    更新日期:2001-05-25 00:00:00

  • Primary structure of matrilin-3, a new member of a family of extracellular matrix proteins related to cartilage matrix protein (matrilin-1) and von Willebrand factor.

    abstract::A mouse cDNA encoding for matrilin-3, the third member of the novel matrilin family of extracellular matrix proteins, was cloned. The protein precursor of 481 amino acids consists of a putative signal peptide, a short positively charged sequence, a single vWFA-like domain followed by four epidermal growth factor-like ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(97)00895-8

    authors: Wagener R,Kobbe B,Paulsson M

    更新日期:1997-08-11 00:00:00

  • Identification of a functional site on the type I TGF-beta receptor by mutational analysis of its ectodomain.

    abstract::Six charged amino acid residues located in the ectodomain of the full-length type I transforming growth factor (TGF)-beta receptor were individually mutated to alanine. Mutation of residues D47, D98, K102 and E104 resulted in functionally impaired receptors as demonstrated by a marked decrease in ligand-dependent sign...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(01)03231-8

    authors: Guimond A,Sulea T,Zwaagstra JC,Ekiel I,O'Connor-McCourt MD

    更新日期:2002-02-27 00:00:00

  • Developmental regulation of expression of the alpha 1 and alpha 2 subunits mRNAs of the voltage-dependent calcium channel in a differentiating myogenic cell line.

    abstract::The voltage-dependent calcium channel (VDCC) in skeletal muscle probably plays a key role in transducing membrane charge movement to the calcium release channel. We report here that the expression of VDCC alpha 1 and alpha 2 mRNAs is developmentally regulated in differentiating C2C12 myogenic cells. The alpha 1 mRNA i...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(89)80787-2

    authors: Varadi G,Orlowski J,Schwartz A

    更新日期:1989-07-03 00:00:00