A novel heterodimeric antimicrobial peptide from the tree-frog Phyllomedusa distincta.

abstract：:We have identified and analyzed a 27-nucleotide sequence (U5 repressive element, designated as U5RE) at the U5 region of the human T-cell leukemia virus type I (HTLV-I) long terminal repeat (LTR) which is required for HTLV-I basal transcriptional repression. The basal promoter strength of constructs that contained del...

journal_title：FEBS letters

authors： Okumura K,Takagi S,Sakaguchi G,Naito K,Minoura-Tada N,Kobayashi H,Mimori T,Hinuma Y,Igarashi H

更新日期：1994-12-12 00:00:00

abstract：:Magainins are a family of antimicrobial peptides present in the skin extracts of Xenopus laevis. Both magainin-1 and -2 do not have any significant effect on the activity of protein kinase C (PKC). Magainin-2 was found to be readily phosphorylated by PKC to 0.5 mol 32P/mol of peptide. Neither magainin-1, which has a s...

journal_title：FEBS letters

authors： Nakabayashi H,Brown JH,Morell JL,Chen HC,Huang KP

更新日期：1990-07-02 00:00:00

abstract：:Mitogen activated protein kinase in extracts of U-937 macrophage-like cells was stimulated by LDL and oxLDL. A maximum value (161% of the basal phosphotransferase activity) was obtained after 6 min exposure to oxidized LDL (27 microgram/ml) using APRTPGGRR peptide substrate. The activatory effect was more pronounced (...

journal_title：FEBS letters

authors： Deigner HP,Claus R

更新日期：1996-05-06 00:00:00

abstract：:Bispecific miniantibodies were constructed by genetically fusing the C(H)1 domain of an IgG1 to the C-terminus of a single-chain Fv fragment (scFv-425), specific for the EGF receptor, and fusing the C(L) domain of a kappa light chain to the C-terminus of a scFv specific for CD2 (scFv-M1). An efficient dicistronic gene...

journal_title：FEBS letters

authors： Müller KM,Arndt KM,Strittmatter W,Plückthun A

更新日期：1998-01-30 00:00:00

abstract：:We have used 19F NMR and CD spectra to study interactions of TFP with CaM under various conditions where no aggregation of TFP occurs. It was found that TFP is bound to CaM in the absence of Ca2+ with Ka greater than 4 X 10(4) M in terms of 19F NMR of TFP and that KCl markedly influences the interaction of TFP with Ca...

journal_title：FEBS letters

authors： Shimizu T,Hatano M

更新日期：1983-08-22 00:00:00

abstract：:Cell-substratum adhesion of rat hepatocytes was inhibited by antisera raised against plasma membranes of liver (anti-liver antiserum) and Morris hepatoma 7777 (anti-hepatoma antiserum). Similar concentrations of both antisera inhibited adhesion on collagen. Anti-liver antiserum also inhibited the adhesion of hepatocyt...

journal_title：FEBS letters

authors： Neumeier R,Dethlefs U,Reutter W

更新日期：1984-03-26 00:00:00

abstract：:Currently described substrates of the bacterial Tat protein transport system are directed for export by signal peptides containing a pair of invariant arginine residues. The signal peptide of the TtrB subunit of Salmonella enterica tetrathionate reductase contains a single arginine residue but is nevertheless able to ...

journal_title：FEBS letters

authors： Hinsley AP,Stanley NR,Palmer T,Berks BC

更新日期：2001-05-18 00:00:00

abstract：:Antifungal activity has been associated with 2 immunochemically distinct proteins, protein R and S (Mr approximately 23 kDa; pI 9-10), which were isolated in pure form from barley grain. The proteins are homologous with thaumatin- and pathogenesis-related proteins of the PR5 family. The proteins inhibit growth of i.a....

journal_title：FEBS letters

authors： Hejgaard J,Jacobsen S,Svendsen I

更新日期：1991-10-07 00:00:00

abstract：:In bovine heart mitochondria a protein of M(r) 18 kDa, phosphorylated by mtPKA, is associated to the NADH-ubiquinone oxidoreductase in the inner membrane and is present in purified preparation of this complex. The 18 kDa phosphoprotein has now been isolated and sequenced. It is identified as the 18 kDa (IP) AQDQ subun...

journal_title：FEBS letters

authors： Papa S,Sardanelli AM,Cocco T,Speranza F,Scacco SC,Technikova-Dobrova Z

更新日期：1996-02-05 00:00:00

abstract：:Human pepsinogen A3 and A5 have been purified to chromatographic and electrophoretic homogeneity. At pH 2 pepsinogen A3 activates at a much faster rate than pepsinogen A5. Leu-23-Lys-24 is the first bond cleaved during activation of pepsinogen A3. This bond is also cleaved in pepsinogen A5, but together with the cleav...

journal_title：FEBS letters

authors： Foltmann B

更新日期：1988-12-05 00:00:00

abstract：:The anomolous protein synthesis, sensitive to cycloheximide, which has been observed in brain mitochondrial and synaptosomal preparations, has been studied. It is concluded that this protein synthesis is due to the presence, as a contaminant in both preparations, of a ribosome-containing particle which contains solubl...

journal_title：FEBS letters

authors： Morgan IG

更新日期：1970-10-16 00:00:00

abstract：:The expression of interphotoreceptor retinoid binding protein (IRBP) is limited to photoreceptor cells of the retina and pinealocytes of the pineal gland. We sought to define cis-elements of the mouse IRBP 5' flanking region that are required for this restricted activity. In vitro transient transfections of retinoblas...

journal_title：FEBS letters

authors： Boatright JH,Knox BE,Jones KM,Stodulkova E,Nguyen HT,Padove SA,Borst DE,Nickerson JM

更新日期：2001-08-24 00:00:00

abstract：:African horse sickness virus (AHSV), a member of the orbivirus genus of the family Reoviridae, is an insect-vectored pathogen of horses of concern to the equine industry. Studies on AHSV replication and pathogenesis have been hampered by the lack of reverse genetics allowing targeted mutation of viral genomes. We demo...

journal_title：FEBS letters

authors： Matsuo E,Celma CC,Roy P

更新日期：2010-08-04 00:00:00

abstract：:L-selectin guides lymphocytes into peripheral lymphoid tissues by recognizing glycoprotein ligands decorated with 6-sulfated sialyl Lewis x (sulfo sLex). Here we have used a rat peripheral lymph node high endothelial cell line (Ax) to study in detail the synthesis, expression and degradation of sLex epitope. We show h...

journal_title：FEBS letters

authors： Majuri ML,Räbinä J,Niittymäki J,Tiisala S,Mattila P,Aavik E,Miyasaka M,Renkonen O,Renkonen R

更新日期：1999-07-16 00:00:00

abstract：:Laminin 5, an anchoring filament protein previously known as nicein/kalinin/epiligrin, consists of three polypeptide chains, alpha 3, beta 3, and gamma 2, encoded by the genes LAMA3, LAMB3, and LAMC2, respectively. The expression of laminin 5 was detected by Northern hybridization with specific cDNA probes in various ...

journal_title：FEBS letters

authors： Korang K,Christiano AM,Uitto J,Mauviel A

更新日期：1995-07-24 00:00:00

abstract：:Glycogen synthase from human and bovine polymorphonuclear leukocytes was purified to homogeneity. Rabbit antisera were raised against the two glycogen synthases and used for immunochemical analysis. Western blotting analysis showed that the subunit of glycogen synthase in crude homogenates of human and bovine leukocyt...

journal_title：FEBS letters

authors： Rasmussen LH,Juhl H,Esmann V,Petersen HU

更新日期：1988-03-28 00:00:00

abstract：:Although neurokinin A (NKA), a tachykinin peptide with sequence homology to substance P (SP), is a weak competitor of radiolabeled SP binding to the NK-1 receptor (NK-1R), more recent direct binding studies using radiolabeled NKA have demonstrated an unexpected high-affinity interaction with this receptor. To document...

journal_title：FEBS letters

authors： Bremer AA,Leeman SE,Boyd ND

更新日期：2000-12-01 00:00:00

abstract：:Data are presented on the formation of a linoleic acid oxygenation product under basal conditions by guinea pig alveolar macrophages (AM). Under non-stimulated conditions 20 X 10(6) AM released about 1 nmol 9-hydroxylinoleic acid (9-HODE) during a 15 min incubation. Furthermore, AM released more than 2 nmol 12-hydroxy...

journal_title：FEBS letters

authors： Engels F,Willems H,Nijkamp FP

更新日期：1986-12-15 00:00:00

abstract：:There is strong evidence of a key role for mitochondrial dysfunction in both sporadic and all forms of familial Parkinson's disease (PD). However, none of the clinical trials carried out with putative mitochondrial rescue agents have been successful. Firm establishment of a wet biomarker or a reliable readout from ima...

journal_title：FEBS letters

authors： Mortiboys H,Macdonald R,Payne T,Sassani M,Jenkins T,Bandmann O

更新日期：2018-03-01 00:00:00

abstract：:Horseradish peroxidase in the presence of hydrogen peroxide (HRP/H2O2) oxidizes a carcinogenic non-aminoazo dye, 1-phenylazo-2-hydroxynaphthalene (Sudan I) to the ultimate carcinogen, which binds to calf thymus DNA. The principal product of Sudan I oxidation by the HRP/H2O2 system is the benzenediazonium ion. Minor pr...

journal_title：FEBS letters

authors： Stiborová M,Asfaw B,Anzenbacher P

更新日期：1988-05-23 00:00:00

abstract：:The Ca(2+)-activated photoprotein, aequorin, contains six tryptophan residues and has a bioluminescence emission maximum at 465 nm. On converting the six tryptophan residues to phenylalanine, the mutant aequorins exhibited varied luminescence activities and spectra, but one mutant, with tryptophan-86 replaced by pheny...

journal_title：FEBS letters

authors： Ohmiya Y,Ohashi M,Tsuji FI

更新日期：1992-04-20 00:00:00

abstract：:We have observed that the extracellular domain of T beta RI and protectin (CD59), an inhibitor of the membrane attack complex of complement, share structural features, a distinct spacing of ten cysteines and a C-terminal 'Cys-box'. Based on these common features and the recently determined NMR-structure of protectin, ...

journal_title：FEBS letters

authors： Jokiranta TS,Tissari J,Teleman O,Meri S

更新日期：1995-11-27 00:00:00

abstract：:Zona pellucida (ZP) binding proteins from boar spermatozoa were compared with antigens recognized by ACR.2 and ACR.3 monoclonal antibodies. The ZP binding proteins of 55, 53, 45 and 38 kDa are identical with various forms of boar acrosin immunologically detected by ACR.2 antibody. The ZP binding proteins of 17 and 18 ...

journal_title：FEBS letters

authors： Moos J,Pĕknicová J,Surneva-Nakova TN,Pavlík M

更新日期：1990-05-21 00:00:00

abstract：:A series of 9-beta-D-xylofuranosyladenine (xyloA or xyloadenosine) substituted analogs of 2-5A core trimer and tetramer were examined for their ability to be degraded by the 2',5'-phosphodiesterase activity of cytoplasmic extracts of mouse L cells. Two distinct groups of xyloA-substituted analogs could be readily disc...

journal_title：FEBS letters

authors： Torrence PF,Alster D,Huss S,Gosselin G,Imbach JL

更新日期：1987-02-23 00:00:00

abstract：:In this paper we introduce monobromobimane, a thiol reagent, as a selective blocker of the recently identified dithiol whose oxidation-reduction status modifies voltage sensing by the mitochondrial permeability transition pore, a cyclosporin A-sensitive channel. Monobromobimane does not inhibit the phosphate carrier, ...

journal_title：FEBS letters

authors： Costantini P,Chernyak BV,Petronilli V,Bernardi P

更新日期：1995-04-03 00:00:00

abstract：:Glucocorticoid-induced TNFR-related protein (GITR) is constitutively expressed in T regulatory (Treg) cells and regulates their suppressive function. We identified two methylated CpG islands in the Gitr locus. Using a ChIP assay, we demonstrate that both DNMT1 and methyl-CpG-binding domain Protein 4 (MBD4) bind to the...

journal_title：FEBS letters

authors： Wang S,Li Y,Zhu F,Lin F,Luo X,Zhao B,Zhang P,Li D,Gao Y,Liang R,Liu L,Tsun A,Yuan X,Wu K,Li B

更新日期：2017-07-01 00:00:00

abstract：:Several deletion mutants of Raf-1 were expressed with v-ras p21 or 14-3-3 protein in COS-7 cells and Sf9 cells and the interaction of Raf-1 with ras p21 or with 14-3-3 protein in intact cells was examined. Raf(1-135) (residues 1-135) and Raf(1-322) interacted with v-ras p21, but other deletion mutants such as Raf(136-...

journal_title：FEBS letters

authors： Koyama S,Williams LT,Kikuchi A

更新日期：1995-07-17 00:00:00

abstract：:The substrate peptide bond cleaved by cathepsins B and L is determined not by the amino acid contributing the carboxyl group to this bond as in the case of serine proteases but rather by the presence of a neighboring amino acid with a large hydrophobic side chain. From a study of the inhibitory potency in a series, Cb...

journal_title：FEBS letters

authors： Kirschke H,Wikstrom P,Shaw E

更新日期：1988-02-08 00:00:00

abstract：:E. coli GroEL chaperonin monomers, isolated after urea-induced dissociation of GroEL14, undergo cold denaturation below 5 degrees C. Above 5 degrees C, these monomers undergo MgATP-dependent self-assembly. We have demonstrated a conformational transition at 0 degree C induced by interaction of monomeric GroEL with ade...

journal_title：FEBS letters

authors： Lissin NM,Hemmingsen SM

更新日期：1993-06-07 00:00:00

abstract：:Chimeric antibodies composed of mouse-derived variable regions and human-derived constant regions have been developed for clinical use. However, construction of chimeric mouse/human genes in expression vectors is time-consuming work. In this study, we developed convenient vectors for construction of chimeric mouse/hum...

journal_title：FEBS letters

authors： Kameyama K,Imai K,Itoh T,Taniguchi M,Miura K,Kurosawa Y

更新日期：1989-02-27 00:00:00