Abstract:
:Glucocorticoid-induced TNFR-related protein (GITR) is constitutively expressed in T regulatory (Treg) cells and regulates their suppressive function. We identified two methylated CpG islands in the Gitr locus. Using a ChIP assay, we demonstrate that both DNMT1 and methyl-CpG-binding domain Protein 4 (MBD4) bind to the Gitr promoter. Moreover, knockdown of DNMT1 decreases the binding activity of MBD4. We observed much higher levels of both DNMT1 and MBD4 in human CD4+ CD25- conventional T (Tconv) cells. Moreover, co-overexpression of DNMT1 and MBD4 in Treg cells significantly inhibits GITR expression and impairs their suppressive activity. Our results reveal a novel molecular mechanism by which MBD4 inhibits GITR expression in a DNMT1-dependent manner.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Wang S,Li Y,Zhu F,Lin F,Luo X,Zhao B,Zhang P,Li D,Gao Y,Liang R,Liu L,Tsun A,Yuan X,Wu K,Li Bdoi
10.1002/1873-3468.12690subject
Has Abstractpub_date
2017-07-01 00:00:00pages
1929-1939issue
13eissn
0014-5793issn
1873-3468journal_volume
591pub_type
信件相关文献
FEBS LETTERS文献大全abstract::Human Tid-1 (hTid-1) is a DnaJ chaperone protein with homology to the Drosophila tumor suppressor Tid56. We report the first case of a tumor-associated mutation at the human TID1 locus, which was identified in the SF767 glioma cell line giving rise to aberrantly high levels of a hTid-1(L) mutant variant. In this study...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.11.034
更新日期:2004-12-17 00:00:00
abstract::This study describes the comparative analysis of two insect recombinant aminotransferases, Aedes aegypti 3-hydroxykynurenine (3-HK) transaminase/alanine glyoxylate aminotransferase (Ae-HKT/AGT) and Drosophila melanogaster serine pyruvate aminotransferase (Dm-Spat), which share 52% identity in their amino acid sequence...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03229-5
更新日期:2002-09-11 00:00:00
abstract::trans-3-Chloroacrylic acid dehalogenase (CaaD) catalyzes the hydrolytic dehalogenation of trans-3-haloacrylates to yield malonate semialdehyde by a mechanism utilizing βPro-1, αArg-8, αArg-11, and αGlu-52. These residues are implicated in a promiscuous hydratase activity where 2-oxo-3-pentynoate is processed to acetop...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.07.006
更新日期:2013-09-02 00:00:00
abstract::Several neurodegenerative disorders are characterized by the accumulation of proteinaceous inclusions in the central nervous system. These inclusions are frequently composed of a mixture of aggregation-prone proteins. Here, we used a bimolecular fluorescence complementation assay to study the initial steps of the co-a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.11.019
更新日期:2012-01-02 00:00:00
abstract::We report here the synthesis of novel 5'-linked oligodeoxynucleotides, both normal phosphodiester and phosphorothioate analogs, in which a covalently attached group at the 5'-terminus is an anthraquinone. These compounds represent a new class of antisense compounds in which the base sequence of the oligodeoxynucleotid...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80626-x
更新日期:1989-06-05 00:00:00
abstract::The central nervous system of the pond snail, Lymnaea stagnalis, contains many large, identified neurons which can be easily manipulated making it an advantageous model system to elucidate in vivo the architecture of neuronal signal transduction pathways. We have isolated three cDNA clones encoding G protein alpha sub...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81474-z
更新日期:1992-12-21 00:00:00
abstract::The anti-ICAM-1 monoclonal antibody F10.2 was conjugated to liposomes to target to cells expressing the cell adhesion molecule ICAM-1. We demonstrate that F10.2 immunoliposomes bind to human bronchial epithelial cells (BEAS-2B) and human umbilical vein endothelial cells (HUVEC) in a specific, dose- and time-dependent ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01350-a
更新日期:1995-01-03 00:00:00
abstract::PTPRO is often silenced by DNA hypermethylation in primary human tumors and cancer cell lines and functions as a tumor suppressor. Here we show that PTPRO is a target of E2F1. In addition, the microRNA cluster miR-17-92, another target of E2F1, participates in PTPRO regulation. PTPRO mRNA was up-regulated during S pha...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.07.017
更新日期:2008-08-20 00:00:00
abstract::The Rad51 gene of Saccharomyces cerevisiae is required for genetic recombination and recombinational repair of DNA strand breaks. In higher eukaryotes Rad51 is essential for embryonic development, and is involved in cell proliferation and DNA repair. Here we show that human Rad51 (HsRad51) is proteolytically cleaved d...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00433-5
更新日期:1998-05-08 00:00:00
abstract::Human hepatocellular carcinoma (HCC) is one of the most common tumors worldwide, in which the genetic mechanisms of oncogenesis are still unclear. To investigate whether the genomic DNA copy number alterations may contribute to primary HCC, the cDNA microarray-based comparative genomic hybridization (CGH) analysis was...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.05.032
更新日期:2006-06-26 00:00:00
abstract::In exocrine acinar cells a variety of neurotransmitters (e.g. acetylcholine) stimulate phosphatidylinositol 4,5-bisphosphate hydrolysis elevating intracellular calcium to activate calcium-dependent membrane currents (outward K+ and inward Cl-). This study shows that in lacrimal acinar cells extracellular application o...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80782-e
更新日期:1990-05-07 00:00:00
abstract::The conversion of the cellular prion protein into the beta-sheet-rich scrapie prion protein is thought to be the key step in the pathogenesis of prion diseases. To gain insight into this structural conversion, we analyzed the intrinsic structural propensity of the amino acid sequence of the murine prion C-terminal dom...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03353-7
更新日期:2002-10-09 00:00:00
abstract::Hst1At (accession number AB018695) was identified from the Arabidopsis thaliana sequencing project on BAC T3F12, and the corresponding cDNA was isolated by reverse transcription-PCR. Southern blot analysis reveals a single copy of this gene. The cDNA encodes a root specific sulfate transporter of 649 amino acids. Hete...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01615-x
更新日期:2000-06-09 00:00:00
abstract::The adipose-derived plasma protein, adiponectin (APN), has various protective effects on cardiovascular diseases. In this study, we show that endogenous APN is required for full cyclooxygenase-2 (COX-2) induction by ischemia-reperfusion injury in the heart in vivo. In rat neonatal cardiac myocytes, APN-induced COX-2 e...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.03.002
更新日期:2008-04-02 00:00:00
abstract::Plastoquinone-9 (PQ-9)-depleted PSII reaction center core complex, consisting of CP47/D1/D2/Cytb-559/I, was isolated from spinach PSII particles. PQ-9, lipids and several proteins were extracted from the original PSII particles and separated by several steps of chromatography to be reconstituted into the isolated comp...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01089-7
更新日期:1994-10-31 00:00:00
abstract::VP64 is widely used as a transcriptional activator to investigate the biological function of genes, but its potential for application in genetic improvement of crops has not been fully investigated. Here, we characterized an OsMYB1R1-VP64 fusion protein that enhanced the grain yield of rice cultivar 'Kita-ake' by 35%....
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12374
更新日期:2016-10-01 00:00:00
abstract::SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)1H-imi dazole) is widely used as a specific inhibitor of p38 mitogen-activated protein kinase (MAPK). Here, we report that SB203580 activates the serine/threonine kinase Raf-1 in quiescent smooth muscle cells in a dose-dependent fashion. The concentr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00034-4
更新日期:1999-02-05 00:00:00
abstract::We have studied functional properties of peptide transport in the pheochromocytoma neuroendocrine cell line from rat. The neutral peptide D-Phe-L-Ala (resistant to hydrolysis) is a good substrate for uptake into these cells. Transport is substantially inhibited by diethylpyrocarbonate pretreatment and is stimulated by...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)03081-2
更新日期:2001-11-23 00:00:00
abstract::A change in the conformational plasticity of α-Synuclein (α-Syn) is hypothesised to be a key step in the pathogenic mechanism of Parkinson's disease (PD). Here, we report the study of extracellular α-Syn interaction with whole cells and membranes isolated from the neuronal SH-SY5Y cells, exploiting NMR and CD spectros...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2015.02.012
更新日期:2015-03-24 00:00:00
abstract::A novel peptidyl-prolyl cis/trans isomerase was isolated from Escherichia coli cell extract and characterized partially. Determination of the molecular mass by electrospray mass spectrometry indicated a protein of 10102 +/- 2 Da, smaller than cyclophilins or FK 506 binding proteins currently known. The specificity con...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)80608-x
更新日期:1994-04-18 00:00:00
abstract::Previous studies using retinal analogs (Wada, A., Sakai, M., Kinumi, T., Tsujimoto, K., Yamauchi, M. and Ito, M. (1994) J. Org. Chem. 59, 6992-6997; Wada, A., Sakai, M., Imamoto, Y., Shichida., Y., Yoshizawa, T. and Ito, M. (1993) Chem. Pharm. Bull. 41, 793-795) revealed that both retinochrome and visual pigments shar...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01004-7
更新日期:1998-09-04 00:00:00
abstract::Anthrax is a severe bacterial infection that occurs when Bacillus anthracis spores gain access into the body and germinate in macrophages, causing septicemia and toxemia. Anthrax toxin is a binary A-B toxin composed of protective antigen (PA), lethal factor (LF), and edema factor (EF). PA mediates the entry of either ...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/s0014-5793(02)03609-8
更新日期:2002-11-20 00:00:00
abstract::Growth of E. coli in the presence of the protonophorous uncoupler pentachlorophenol is shown to strongly enhance levels of cytochrome d, a putative Na(+)-motive oxidase. This effect was found to be arrested by chloramphenicol and stimulated by high Na+ concentration in the growth medium. The induction of cytochrome d ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81612-4
更新日期:1993-12-20 00:00:00
abstract::Peptide T, an octapeptide of sequence ASTTTNYT that binds to human T cells, was studied as a zwitterion in DMSOd6 solution by means of proton NMR spectroscopy at 500 MHz. The unusual dispersion of the resonances of residues of the same type (T) makes it possible to assign all resonances to specific residues by means o...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80723-3
更新日期:1988-04-11 00:00:00
abstract::Hydrolysis of GTP, bound to members of the G-protein superfamily, terminates their downstream signaling activity. A conserved glutamine serves a critical role in this pivotal guanosine triphosphatase (GTPase) reaction. However, the role of the catalytic glutamine in GTP hydrolysis is still not well understood. We have...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00930-2
更新日期:1998-08-21 00:00:00
abstract::Mutations of the cysteines (positions 668 and 714) were generated in the IF2 C domain of Bacillus stearothermophilus translation initiation factor IF2. The corresponding proteins were characterized functionally and structurally. Most (yet not all) amino acid replacements at both positions resulted in severe reduction ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01280-6
更新日期:1999-10-15 00:00:00
abstract::The operation of the human red cell glucose transporter has been studied at normal and high hydrostatic pressure to identify the step(s) which involve a volume change. Pressure inhibited zero-trans and equilibrium exchange influx to similar extents, by decreasing the Vmax but not significantly changing the Km. The Bma...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80261-e
更新日期:1992-04-27 00:00:00
abstract::We report here the molecular cloning, nucleotide sequence, and predicted amino acid sequence of an alpha-subunit of the developmentally useful model, Artemia. The amino acid sequence shows divergence from that of mammals, birds, Torpedo, and Drosophila. However, regions in the putative ATP binding and transmembrane do...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81816-2
更新日期:1989-10-23 00:00:00
abstract::In our study, transcriptome microarrays are used to identify differentially expressed miRNAs and mRNAs in cervical cancer specimens. We find that microRNA-145 (miR-145) expression is significantly decreased in cervical cancer tissues and cell lines, and is associated with advanced cancer stages, large tumor size and m...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2015.01.037
更新日期:2015-03-12 00:00:00
abstract::The farnesoid X receptor (FXR) is a nuclear receptor activated by bile acids (BAs). In response to ligand-binding, FXR regulates many genes involved in BA, lipid, and lipoprotein metabolism. To identify new FXR target genes, microarray technology was used to profile total RNA extracted from HepG2 cells treated with th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.04.026
更新日期:2004-05-21 00:00:00