Abstract:
:Human Tid-1 (hTid-1) is a DnaJ chaperone protein with homology to the Drosophila tumor suppressor Tid56. We report the first case of a tumor-associated mutation at the human TID1 locus, which was identified in the SF767 glioma cell line giving rise to aberrantly high levels of a hTid-1(L) mutant variant. In this study, we set out to determine whether this change in hTid-1 status influences the response of glioma cells to adenoviral (Ad)-mediated delivery of the two major isoforms of TID1, hTid-1(L) and hTid-1(S). Ad-hTid-1(S) induced apoptosis in hTid-1 mutant SF767 cells, while causing growth arrest in wild-type hTid-1-expressing U373 and U87 cells. By contrast, Ad-hTid-1(L) infection had no apparent effect on glioma cell growth. The apoptosis induced by hTid-1(S) was accompanied by mitochondrial cytochrome C release and caspase activation and blocked by stable overexpression of Bcl-X(L). Our findings suggest that the status of hTid-1 in gliomas may contribute to their susceptibility to cell death triggers.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Trentin GA,He Y,Wu DC,Tang D,Rozakis-Adcock Mdoi
10.1016/j.febslet.2004.11.034keywords:
subject
Has Abstractpub_date
2004-12-17 00:00:00pages
323-30issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(04)01415-2journal_volume
578pub_type
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