Abstract:
:We have cloned and characterized four Itpk genes from soybean. All four recombinant Itpk proteins showed canonical Ins(1,3,4)P3 5/6-kinase activity, but a kinetic analysis raised questions about its biological significance. Instead, we provide evidence that one alternative biological role for soybean Itpks is to interconvert the Cl(-) channel inhibitor, Ins(3,4,5,6)P4, and its metabolic precursor, Ins(1,3,4,5,6)P5, within a substrate cycle. The soybean Itpks also phosphorylated Ins(3,4,6)P3 to Ins(1,3,4,6)P4 which was further phosphorylated to Ins(1,3,4,5,6)P5 by soybean Ipk2. Thus, soybean Itpks may participate in an inositol lipid-independent pathway of InsP6 synthesis.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Stiles AR,Qian X,Shears SB,Grabau EAdoi
10.1016/j.febslet.2008.04.054subject
Has Abstractpub_date
2008-06-11 00:00:00pages
1853-8issue
13eissn
0014-5793issn
1873-3468pii
S0014-5793(08)00387-6journal_volume
582pub_type
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