Abstract:
:The metallochromic indicator murexide has been used to monitor calcium concentration changes during the dextran-induced, phosphatidylserine-dependent degranulation of rat peritoneal mast cells. The dextran-induced Ca2+-uptake showed an absolute dependence on the presence of phosphatidylserine. The extent of Ca2+-uptake increased with phosphatidylserine in a concentration-dependent manner. At 25 degrees C the half-life of the uptake process equalled 35 +/- 5 s. Exposure of the mast cells to dextran in the presence of Ca2+, but in the absence of phosphatidylserine, desensitized the cells. The subsequent addition of phosphatidylserine failed to restore the Ca2+-uptake activity. However, the Ca2+-ionophore A23187 did promote Ca2+ uptake by the cells without PS.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Sagi-Eisenberg R,Geller-Bernstein C,Ben-Neriah Z,Pecht Idoi
10.1016/0014-5793(83)80725-xsubject
Has Abstractpub_date
1983-09-05 00:00:00pages
37-40issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(83)80725-Xjournal_volume
161pub_type
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