Ca2+-modulated ONE-GC odorant signal transduction.

abstract：:The 21-residue peptide KLLLLKLLLLKLLLLKLLLLK (KL4) has been synthesized and analyzed regarding its secondary structure and orientation in lipid environments. Fourier transform infrared and circular dichroism spectroscopy shows that the peptide exhibits approximately 80% alpha-helical content both in dodecylphosphochol...

journal_title：FEBS letters

authors： Gustafsson M,Vandenbussche G,Curstedt T,Ruysschaert JM,Johansson J

更新日期：1996-04-15 00:00:00

abstract：:Adrenal medullary homogenates and chromaffin granule membranes were separated by SDS-polyacrylamide gel electrophoresis and GTP-binding proteins detected using [alpha-32P]GTP binding to nitrocellulose blots. Four GTP-binding polypeptides of 24, 22, 20 and 18 kDa were routinely found in medullary homogenates and all we...

journal_title：FEBS letters

authors： Burgoyne RD,Morgan A

更新日期：1989-03-13 00:00:00

abstract：:For good function, membrane lipids have to be arranged appropriately and be in the correct physical state. In poikilotherms, exposure to cold stress or heat shock can alter membrane properties such that, unless they are corrected quickly, damage and, possibly, death can result. Low temperature stress is countered by m...

journal_title：FEBS letters

authors： Guschina IA,Harwood JL

更新日期：2006-10-09 00:00:00

abstract：:Those aerobic archaea whose genomes have been sequenced possess a single 4-gene operon that, by sequence comparisons with Bacteria and Eukarya, appears to encode the three component enzymes of a 2-oxoacid dehydrogenase multienzyme complex. However, no catalytic activity of any such complex has ever been detected in th...

journal_title：FEBS letters

authors： Heath C,Jeffries AC,Hough DW,Danson MJ

更新日期：2004-11-19 00:00:00

abstract：:Gustatory papillae and associated taste buds receive and process chemical information from the environment. In mammals, their development takes place during the late phase of embryogenesis. However, the cellular factors that regulate the differentiation of taste papillae remain largely unknown. Here, we show by quanti...

journal_title：FEBS letters

authors： Ortíz-Alvarado R,Guzmán-Quevedo O,Mercado-Camargo R,Haertle T,Vignes C,Bolaños-Jiménez F

更新日期：2006-10-02 00:00:00

abstract：:Extraction and identification of the non-covalently bound heme groups from crude membrane preparations of photoheterotrophically grown Synechocystis sp. PCC 6803 by reversed phase high performance liquid chromatography and optical spectrophotometry led to the detection of heme O in addition to hemes B and A which latt...

journal_title：FEBS letters

authors： Peschek GA,Wastyn M,Fromwald S,Mayer B

更新日期：1995-09-04 00:00:00

abstract：:VinJ is an amidohydrolase belonging to the serine peptidase family that catalyzes the hydrolysis of the terminal aminoacyl moiety of a polyketide intermediate during the biosynthesis of vicenistatin. Herein, we report the crystal structure of VinJ. VinJ possesses a unique hydrophobic tunnel for the recognition of the ...

journal_title：FEBS letters

authors： Shinohara Y,Miyanaga A,Kudo F,Eguchi T

更新日期：2014-03-18 00:00:00

abstract：:Two RNA silencing-related phenomena, quelling and meiotic silencing by unpaired DNA (MSUD) have been identified in the fungus Neurospora crassa. Similar to the case with the siRNA and miRNA pathways in Drosophila, different sets of protein components including RNA-dependent RNA polymerase, argonaute and dicer, are use...

journal_title：FEBS letters

authors： Nakayashiki H

更新日期：2005-10-31 00:00:00

abstract：:2-thiouracil (TU), an established antithyroid drug and melanoma-seeker, was found to selectively inhibit neuronal nitric oxide synthase (nNOS) in a competitive manner (K(i)=20 microM), being inactive on the other NOS isoforms. The drug apparently interfered with the substrate- and tetrahydrobiopterin (BH(4))-binding t...

journal_title：FEBS letters

authors： Palumbo A,d'Ischia M,Cioffi FA

更新日期：2000-11-24 00:00:00

abstract：:We investigated the effect of hydroxyl substituted chalcone (1a) and some chalcone analogues (1b-d) on isolated rat liver mitochondria to gain new insights into the cytotoxic mechanism of these compounds. We observed an inhibitory effect on phosphorylation and the partial uncoupling of compounds 1a and 1d. Increased r...

journal_title：FEBS letters

authors： Guzy J,Vasková-Kubálková J,Rozmer Z,Fodor K,Mareková M,Poskrobová M,Perjési P

更新日期：2010-02-05 00:00:00

abstract：:Members of the heat shock protein 70 (Hsp70) family are found in most of the compartments of eukaryotic cells where they play essential roles in protein metabolism. In yeast mitochondria, two Hsp70 proteins are known: Ssc1 and Ssq1. We identified Ecm10 as a third Hsp70 protein in the mitochondrial matrix. Ecm10 shares...

journal_title：FEBS letters

authors： Baumann F,Milisav I,Neupert W,Herrmann JM

更新日期：2000-12-29 00:00:00

abstract：:Stimulation of the bicarbonate dehydration reaction in thylakoid suspension under conditions of saturating light at pH 7.6-8.0 was discovered. This effect was inhibited by nigericin or the lipophilic carbonic anhydrase (CA) inhibitor ethoxyzolamide (EZ), but not by the hydrophilic CA inhibitor, acetazolamide. It was s...

journal_title：FEBS letters

authors： Moskvin OV,Ivanov BN,Ignatova LK,Kollmeier MA

更新日期：2000-03-31 00:00:00

abstract：:Lung surfactant secretion is a highly regulated process. Our previous studies have shown that VAMP-2 is essential for surfactant secretion. In the present study we investigated the role of miR-206 in surfactant secretion through VAMP-2. VAMP-2 was confirmed to be a target of miR-206 by 3'-untranslational region (3'-UT...

journal_title：FEBS letters

authors： Zhang H,Guo Y,Mishra A,Gou D,Chintagari NR,Liu L

更新日期：2015-01-02 00:00:00

abstract：:The photocycle of the light-adapted purple membrane was studied with a time-resolved fluorometry apparatus: fluorescence of the sample suspension (greater than 660 nm) was pumped with a 633-nm cw laser and the temporal change induced by a 532-nm pulsed laser was measured with a photon-counting-type transient recorder....

journal_title：FEBS letters

authors： Ohtani H,Itoh H,Shinmura T

更新日期：1992-06-22 00:00:00

abstract：:Plastidic type I signal peptidase 1 (Plsp1) is necessary for proper chloroplast development although its mode of action is not completely understood. Here, we demonstrate by immunoblotting and electron microscopy immunolocalization that Plsp1 is evenly distributed in the envelope and thylakoids of developing chloropla...

journal_title：FEBS letters

authors： Shipman RL,Inoue K

更新日期：2009-03-04 00:00:00

abstract：:The STARD1 subfamily of 'START' lipid trafficking proteins can reduce macrophage lipid content and inflammatory status (STARD1; StAR), and traffic cholesterol from endosomes (STARD3/MLN64). During macrophage differentiation, STARD1 mRNA and protein increase with sterol content, while the reverse is true for STARD3. St...

journal_title：FEBS letters

authors： Borthwick F,Taylor JM,Bartholomew C,Graham A

更新日期：2009-04-02 00:00:00

abstract：:The covalent conjugation of oligonucleotides to antibody Fab' fragments was optimized by using oligonucleotides modified with a hexaethylene linker arm bearing three amino groups. One oligonucleotide was coupled to antibody of one specificity and a complementary oligonucleotide to antibody of a second specificity. The...

journal_title：FEBS letters

authors： Chaudri ZN,Bartlet-Jones M,Panayotou G,Klonisch T,Roitt IM,Lund T,Delves PJ

更新日期：1999-04-30 00:00:00

abstract：:Calmodulin is shown to interact with human spectrin dimer. The binding was highly calcium-dependent and observed in two different kinds of experiments. Firstly, affinity chromatography of calmodulin on a Sepharose 4B column with immobilized spectrin, and secondly, partition in aqueous two-phase polymer systems. In the...

journal_title：FEBS letters

authors： Berglund A,Backman L,Shanbhag VP

更新日期：1984-06-25 00:00:00

abstract：:The fluorescent Ca2+ indicator Fura 2 was used to monitor Ca2+ release induced by the Ins(1,4,5)P3-mobilizing agonist angiotensin II (Ag II), caffeine and 2,5-di-(tert-butyl)-1,4-benzohydroquinone (tuBHQ), in intact bovine adrenal chromaffin cells. Under low external Ca2+ conditions, tuBHQ, Ag II and caffeine elicited...

journal_title：FEBS letters

authors： Robinson IM,Burgoyne RD

更新日期：1991-09-09 00:00:00

abstract：:To investigate the cis-acting DNA elements that are involved in the regulation of class I major histocompatibility complex genes by interferon, several promoter fragments of the H-2Kk gene were linked to the reporter chloramphenicol acetyl transferase (CAT) gene, and the CAT expression was analyzed in stable transfect...

journal_title：FEBS letters

authors： Pascucci A,Pannuti A,La Mantia G,Lania L

更新日期：1988-01-04 00:00:00

abstract：:A number of recent observations have suggested that the endocytic and biosynthetic pathways may share fundamentally similar transport mechanisms at the molecular level. Some of the more striking of these suggestions have come from a comparison of the effects of the macrocyclic lactone brefeldin A (BFA) on endosomes an...

journal_title：FEBS letters

authors： Hunziker W,Whitney JA,Mellman I

更新日期：1992-07-27 00:00:00

abstract：:The interferon inducible transmembrane (IFITM) proteins mediate several cellular processes such as homotypic cell adhesion functions of interferons (IFNs) and cellular anti-proliferative activities. We show that the BAF complex-mediated induction of IFITM3 is dependent on binding of the transcriptional enhancer factor...

journal_title：FEBS letters

authors： Cuddapah S,Cui K,Zhao K

更新日期：2008-01-23 00:00:00

abstract：:Msn2 and Msn4 transcription factors activate expression of stress-responsive element (STRE) controlled genes in response to various stresses triggering the environmental stress response in Saccharomyces cerevisiae. Although high hydrostatic pressure is known to induce gene expression modification in yeast, the transcr...

journal_title：FEBS letters

authors： Domitrovic T,Fernandes CM,Boy-Marcotte E,Kurtenbach E

更新日期：2006-11-13 00:00:00

abstract：:The cDNA of the human muscle type acylphosphatase was isolated and characterized. The mRNA presents a very long 5'-untranslated region, covering the first half of the molecule: 175 bases of this part were cloned and prediction of the possible secondary structure showed that a very stable stem-loop structure could be f...

journal_title：FEBS letters

authors： Fiaschi T,Marzocchini R,Raugei G,Veggi D,Chiarugi P,Ramponi G

更新日期：1997-11-03 00:00:00

abstract：:Interaction of the water oxidising manganese complex of photosystem II with the aqueous environment has been investigated using electron paramagnetic resonance spectroscopy and electron spin echo envelope modulation spectroscopy to detect interaction of [2H]methanol with the complex in the S2 state. The experiments sh...

journal_title：FEBS letters

authors： Evans MC,Gourovskaya K,Nugent JH

更新日期：1999-05-07 00:00:00

abstract：:ent-Kaurene is the key intermediate in biosynthesis of gibberellins (GAs), plant hormones. In higher plants, ent-kaurene is synthesized successively by copalyl diphosphate synthase (CPS) and ent-kaurene synthase (KS) from geranylgeranyl diphosphate (GGDP). On the other hand, fungal ent-kaurene synthases are bifunction...

journal_title：FEBS letters

authors： Hayashi K,Kawaide H,Notomi M,Sakigi Y,Matsuo A,Nozaki H

更新日期：2006-11-13 00:00:00

abstract：:Molybdenum is an essential micronutrient required by plants. The mechanism of molybdenum uptake in plants is poorly understood, however, evidence has suggested that sulfate transporters may be involved. The sulfate transporter from Stylosanthes hamata, SHST1, restored growth of the sulfate transport yeast mutant, YSD1...

journal_title：FEBS letters

authors： Fitzpatrick KL,Tyerman SD,Kaiser BN

更新日期：2008-04-30 00:00:00

abstract：:Although initially viewed as housekeeping enzymes, research over the last 15 years has revealed that the protein tyrosine phosphatases (PTPs) are critical regulators of tyrosine phosphorylation-dependent signaling events and may represent novel targets for therapeutic intervention in a variety of human diseases. In th...

journal_title：FEBS letters

authors： Tonks NK

更新日期：2003-07-03 00:00:00

abstract：:The effect of exogenous gangliosides on the occurrence of domes in MDCK cell cultures was investigated in view of the involvement of both dome formation and gangliosides in cell growth, differentiation and transepithelial transport. Dome formation was increased by gangliosides in medium free of fetal calf serum. Among...

journal_title：FEBS letters

authors： Rodrig N,Osanai T,Iwamori M,Nagai Y

更新日期：1987-09-14 00:00:00

abstract：:Caloric restriction (CR) is the most compelling example of lifespan extension by external manipulation. Although the molecular mechanisms remain unknown, the theory of hormesis has been invoked to explain the life promoting effects of CR. Hormesis is defined as the beneficial effects of low intensity stressor on a cel...

journal_title：FEBS letters

authors： Kharade SV,Mittal N,Das SP,Sinha P,Roy N

更新日期：2005-12-19 00:00:00