Abstract:
:The fluorescent Ca2+ indicator Fura 2 was used to monitor Ca2+ release induced by the Ins(1,4,5)P3-mobilizing agonist angiotensin II (Ag II), caffeine and 2,5-di-(tert-butyl)-1,4-benzohydroquinone (tuBHQ), in intact bovine adrenal chromaffin cells. Under low external Ca2+ conditions, tuBHQ, Ag II and caffeine elicited Ca2+ rises, indicating Ca2+ release from internal stores. Prior addition of Ag II had no noticeable effect on the extent of release of Ca2+ induced by tuBHQ. Stimulation of the cells with tuBHQ before either Ag II or caffeine, similarly had no effect on Ca2+ released by these two agonists. It was concluded, therefore, that there is a third intracellular Ca2+ store in bovine adrenal chromaffin cells, distinct and non-overlapping, from those sensitive to caffeine or Ins(1,4,5)P3-mobilizing agonists.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Robinson IM,Burgoyne RDdoi
10.1016/0014-5793(91)81057-fsubject
Has Abstractpub_date
1991-09-09 00:00:00pages
151-4issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(91)81057-Fjournal_volume
289pub_type
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