Abstract:
:The cDNA of the human muscle type acylphosphatase was isolated and characterized. The mRNA presents a very long 5'-untranslated region, covering the first half of the molecule: 175 bases of this part were cloned and prediction of the possible secondary structure showed that a very stable stem-loop structure could be formed in that region. Moreover, an additional AUG triplet was found upstream of the start codon of the protein, defining an open reading frame of 60 codons which overlapped that of acylphosphatase. The possible regulatory effect on translation of this part of the mRNA molecule was studied by means of transient transfection experiments: a 10-fold decrease in the expression of a reporter protein and a dramatic decrease in the corresponding mRNA was observed, due to the presence of the 5'-untranslated region of acylphosphatase mRNA. Mutagenesis of the upstream AUG triplet eliminated mRNA instability, leading to the hypothesis that the product of the upstream open reading frame could play a role in this mechanism.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Fiaschi T,Marzocchini R,Raugei G,Veggi D,Chiarugi P,Ramponi Gdoi
10.1016/s0014-5793(97)01270-2subject
Has Abstractpub_date
1997-11-03 00:00:00pages
130-4issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(97)01270-2journal_volume
417pub_type
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