Biochemical evidence for the interaction of regulatory subunit of cAMP-dependent protein kinase with IDA (Inter-DFG-APE) region of catalytic subunit.


:To explore the structural basis required for the holoenzyme formation of cAMP-dependent protein kinase, we have prepared rabbit anti-peptide antibodies that can block the holoenzyme formation without affecting the catalytic activity of the enzyme. The antibodies were raised against a specific site in the catalytic (C)-subunit, termed IDA (Inter-DFG-APE) region, which lies between the kinase subdomains VII and VIII. Although the C-subunit immunoprecipitated with anti-IDA antibodies could not form a stable complex with regulatory (R)-subunit, it was still susceptible to inhibition by the R-subunit or by PKI, a specific inhibitor peptide containing a pseudosubstrate site. These results indicate that there exists an IDA region-mediated interaction between the R- and C-subunits, which is distinct from that mediated through the substrate site and substrate binding site. In accordance with this idea, association of synthetic IDA peptides with the R-subunit was directly demonstrated by resonance mirror analysis. The calculated association constants of IDA peptides were high enough to suggest a possible involvement of the IDA region in the initial step of holoenzyme formation.




FEBS letters


Sahara S,Sato K,Kaise H,Mori K,Sato A,Aoto M,Tokmakov AA,Fukami Y




Has Abstract


1996-04-15 00:00:00














  • Placental alkaline phosphatase activity is inversely related to cell growth rate in HeLaS3 cervical cancer cells.

    abstract::Placental alkaline phosphatase is an inducible enzyme, expressed in HeLaS3 cells, which has been shown to possess protein phosphotyrosine phosphatase activity. Since phosphotyrosine levels are known to increase in actively dividing cells we sought an inverse correlation between PLAP activity and growth rate in HeLaS3 ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Telfer JF,Green CD

    更新日期:1993-08-30 00:00:00

  • Membrane interaction of TNF is not sufficient to trigger increase in membrane conductance in mammalian cells.

    abstract::Tumor necrosis factor TNF can trigger increases in membrane conductance of mammalian cells in a receptor-independent manner via its lectin-like domain. A lectin-deficient TNF mutant, lacking this activity, was able to bind to artificial liposomes in a pH-dependent manner, but not to insert into the bilayer, just like ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: van der Goot FG,Pugin J,Hribar M,Fransen L,Dunant Y,De Baetselier P,Bloc A,Lucas R

    更新日期:1999-10-22 00:00:00

  • Ser727-dependent transcriptional activation by association of p300 with STAT3 upon IL-6 stimulation.

    abstract::Activation of the signal transducer and activator of transcription 3 (STAT3) in response to interleukin-6 (IL-6) type cytokines involves both phosphorylation of Tyr705, which enables dimerization, nuclear translocation and DNA binding, as well as ser727 phosphorylation. Here, we describe that the 65 C-terminal amino a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Schuringa JJ,Schepers H,Vellenga E,Kruijer W

    更新日期:2001-04-20 00:00:00

  • Mutation of an evolutionarily conserved tyrosine residue in the active site of a human class Alpha glutathione transferase.

    abstract::Human class Alpha glutathione transferase (GST) A1-1 has been subjected to site-directed mutagenesis of a Tyr residue conserved in all classes of cytosolic GSTs. The change of Tyr8----Phe lowers the specific activities with three substrates to 2-8% of the values for the wild-type enzyme. The changes in the kinetic par...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Stenberg G,Board PG,Mannervik B

    更新日期:1991-11-18 00:00:00

  • Glutathione depletion induces glycogenolysis dependent ascorbate synthesis in isolated murine hepatocytes.

    abstract::The relationship between glutathione deficiency, glycogen metabolism and ascorbate synthesis was investigated in isolated murine hepatocytes. Glutathione deficiency caused by various agents increased ascorbate synthesis with a stimulation of glycogen breakdown. Increased ascorbate synthesis from UDP-glucose or gulonol...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Braun L,Csala M,Poussu A,Garzó T,Mandl J,Bánhegyi G

    更新日期:1996-06-17 00:00:00

  • Intracellular distribution of DNA methyltransferase during the cell cycle.

    abstract::The intracellular distribution of DNA methyltransferase has been analyzed in synchronously proliferating human cells. The localization of DNA methyltransferase was determined immunocytochemically using monoclonal antibodies directed against this enzyme. DNA methyltransferase was found to accumulate predominantly in nu...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Vogel MC,Papadopoulos T,Müller-Hermelink HK,Drahovsky D,Pfeifer GP

    更新日期:1988-08-15 00:00:00

  • ATP synthesis catalyzed by the mitochondrial F1-F0 ATP synthase is not a reversal of its ATPase activity.

    abstract::The ADP(Mg2+)-deactivated oligomycin-sensitive F1-F0 ATPase of coupled submitochondrial particles treated with the substoichiometric amount of oligomycin was studied to test whether ATP synthesis and hydrolysis proceed in either direction through the same intermediates. The initial rates of ATP hydrolysis, oxidative p...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Syroeshkin AV,Vasilyeva EA,Vinogradov AD

    更新日期:1995-06-05 00:00:00

  • Leptospiral TlyC is an extracellular matrix-binding protein and does not present hemolysin activity.

    abstract::The role of TlyA, TlyB and TlyC proteins in the biology of Leptospira is still uncertain. Although these proteins have been considered as putative hemolysins, we demonstrate that leptospiral recombinant TlyB and TlyC do not possess hemolytic activity. However, further experiments showed that TlyC is a surface-exposed ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Carvalho E,Barbosa AS,Gómez RM,Cianciarullo AM,Hauk P,Abreu PA,Fiorini LC,Oliveira ML,Romero EC,Gonçales AP,Morais ZM,Vasconcellos SA,Ho PL

    更新日期:2009-04-17 00:00:00

  • Both bulk and cancer stem cell subpopulations in triple-negative breast cancer are susceptible to Wnt, HDAC, and ERα coinhibition.

    abstract::Development of targeted therapies for triple-negative breast cancer (TNBC, a more aggressive subtype) is an unmet medical need. We analyzed data from 887 patients with invasive breast cancer and observed that increased Wnt and histone deacetylase (HDAC) activities are associated with estrogen receptor 1 (ESR1) and pro...

    journal_title:FEBS letters

    pub_type: 信件


    authors: Sulaiman A,Sulaiman B,Khouri L,McGarry S,Nessim C,Arnaout A,Li X,Addison C,Dimitroulakos J,Wang L

    更新日期:2016-12-01 00:00:00

  • Modification of serine 392 is a critical event in the regulation of p53 nuclear export and stability.

    abstract::Although it has been shown that phosphorylations of p53 serine its residues are critical events for the regulation of their function, the specific biological effects of each of these phosphorylations, especially at serine 392, remain to be elucidated. Serine 392 has been proposed to play a role in the tetramerization ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kim YY,Park BJ,Kim DJ,Kim WH,Kim S,Oh KS,Lim JY,Kim J,Park C,Park SI

    更新日期:2004-08-13 00:00:00

  • Bidirectional communication between sensory neurons and osteoblasts in an in vitro coculture system.

    abstract::Recent studies have revealed that the sensory nervous system is involved in bone metabolism. However, the mechanism of communication between neurons and osteoblasts is yet to be elucidated. In this study, we investigated the signaling pathways between sensory neurons of the dorsal root ganglion (DRG) and the osteoblas...

    journal_title:FEBS letters

    pub_type: 信件


    authors: Kodama D,Hirai T,Kondo H,Hamamura K,Togari A

    更新日期:2017-02-01 00:00:00

  • Localization and in vitro binding studies suggest that the cytoplasmic/nuclear tobacco lectin can interact in situ with high-mannose and complex N-glycans.

    abstract::The possible in vivo interaction of the Nicotiana tabacum agglutinin (Nictaba) with endogenous glycoproteins was corroborated using a combination of confocal/electron microscopy of an EGFP-Nictaba fusion protein expressed in tobacco Bright Yellow-2 (BY-2) cells and biochemical analyses. In vitro binding studies demons...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lannoo N,Peumans WJ,Pamel EV,Alvarez R,Xiong TC,Hause G,Mazars C,Van Damme EJ

    更新日期:2006-11-27 00:00:00

  • The TRAF6 RING finger domain mediates physical interaction with Ubc13.

    abstract::Tumor necrosis factor receptor associated factor 6 (TRAF6) is an important signaling molecule involved in a diverse array of physiological processes. It has been proposed that TRAF6, a RING finger-containing protein, acts as a ubiquitin ligase (E3) and a target for Lys-63 linked polyubiquitination mediated by Ubc13-Ue...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wooff J,Pastushok L,Hanna M,Fu Y,Xiao W

    更新日期:2004-05-21 00:00:00

  • Evidence for a role of C-terminus in Ca(2+) inactivation of skeletal muscle Ca(2+) release channel (ryanodine receptor).

    abstract::Six chimeras of the skeletal muscle (RyR1) and cardiac muscle (RyR2) Ca(2+) release channels (ryanodine receptors) previously used to identify RyR1 dihydropyridine receptor interactions [Nakai et al. (1998) J. Biol. Chem. 273, 13403] were expressed in HEK293 cells to assess their Ca(2+) dependence in [(3)H]ryanodine b...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nakai J,Gao L,Xu L,Xin C,Pasek DA,Meissner G

    更新日期:1999-10-08 00:00:00

  • Solubilisation partial characterisation of the alpha-MSH receptor on primary rat Schwann cells.

    abstract::The ACTH/MSH melanocortin core peptide sequence possesses neurotrophic properties in peripheral nerve. During functional neuroanatomical recovery after damage to peripheral nerves, Schwann cells play a significant role in facilitating regeneration. Here we employ a modified super-potent alpha-MSH analogue to solubilis...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Dyer JK,Ahmed AR,Oliver GW,Poulton CW,Haynes LW

    更新日期:1993-12-20 00:00:00

  • Transforming growth factor-beta inhibits nephronectin-induced osteoblast differentiation.

    abstract::We used cDNA microarray to identify transforming growth factor beta (TGF-beta) responsive target genes during osteoblast development and found that nephronectin (Npnt) is one such gene that is significantly down-regulated. Here we report the role of TGF-beta in regulating Npnt-mediated osteoblast differentiation. We f...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Fang L,Kahai S,Yang W,He C,Seth A,Peng C,Yang BB

    更新日期:2010-07-02 00:00:00

  • A simple method for determining kinetic constants of complexing inactivation at identical enzyme and inhibitor concentrations.

    abstract::The kinetics of complexing inactivation at identical enzyme and inhibitor concentrations were analyzed and the equations of product generation were derived when the free enzyme concentration is great, larger or smaller than the dissociation constant of inhibitor, K(I). The kinetic constants can be obtained by fitting ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wang MH,Zhao KY

    更新日期:1997-08-04 00:00:00

  • Using lipoate enantiomers and thioredoxin to study the mechanism of the 2-oxoacid-dependent dihydrolipoate production by the 2-oxoacid dehydrogenase complexes.

    abstract::The thioredoxin-catalyzed insulin reduction by dihydrolipoate was applied to study the 2-oxoacid: lipoate oxidoreductase activity of 2-oxoacid dehydrogenase complexes. The enzymatic and non-enzymatic mechanisms of the transfer of reducing equivalents from the complexes to free lipoic acid (alpha-lipoic acid, 6,8-thioo...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bunik V,Shoubnikova A,Loeffelhardt S,Bisswanger H,Borbe HO,Follmann H

    更新日期:1995-09-04 00:00:00

  • New polypeptide components purified from mamba venom.

    abstract::New polypeptide components have been isolated from Dendroaspis angusticeps venom using chromatography. Two polypeptides containing 59 and 57 amino acids, called 'DaE1' and 'DaE2' respectively, have been purified to homogeneity and fully sequenced. Spectrometric analysis yielded masses of 6631.5 and 6389.0 Da, respecti...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tytgat J,Vandenberghe I,Ulens C,Van Beeumen J

    更新日期:2001-03-02 00:00:00

  • Calnexin Delta 185-520 partially reverses the misprocessing of the Delta F508 cystic fibrosis transmembrane conductance regulator.

    abstract::Abnormal retention of Delta F508 CFTR (cystic fibrosis transmembrane conductance regulator) in the endoplasmic reticulum is a major cause of cystic fibrosis (CF). We show that calnexin Delta 185-520 but not calnexin can partially reverse the mislocalization of Delta F508 CFTR. This 256-amino acid protein has neither t...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Okiyoneda T,Wada I,Jono H,Shuto T,Yoshitake K,Nakano N,Nagayama S,Harada K,Isohama Y,Miyata T,Kai H

    更新日期:2002-08-28 00:00:00

  • Effects of Ca2+ and a phorbol ester on insulin secretion from islets of Langerhans permeabilised by high-voltage discharge.

    abstract::Isolated rat islets of Langerhans permeabilised by high-voltage discharge secreted insulin in response to elevations in Ca2+ over the range 100 nM to 10 microM Ca2+. The phorbol ester, 12-O-tetradecanoylphorbol 13-acetate (TPA), had no effects on insulin secretion in the absence of Ca2+. In the presence of Ca2+ concen...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Jones PM,Stutchfield J,Howell SL

    更新日期:1985-10-21 00:00:00

  • Identification of the nuclear receptor CAR:HSP90 complex in mouse liver and recruitment of protein phosphatase 2A in response to phenobarbital.

    abstract::The nuclear receptor CAR, a phenobarbital (PB)-responsive transcription factor, translocates into the nucleus of hepatocytes after PB induction. In non-induced mice, CAR forms a physical complex with heat shock protein 90 (HSP90) in the cytoplasm. In response to PB induction, protein phosphatase 2A is recruited to the...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Yoshinari K,Kobayashi K,Moore R,Kawamoto T,Negishi M

    更新日期:2003-07-31 00:00:00

  • Identification of plasma membrane associated mature beta-hexosaminidase A, active towards GM2 ganglioside, in human fibroblasts.

    abstract::Mature beta-hexosaminidase A has been found associated to the external leaflet of plasma membrane of cultured fibroblasts. The plasma membrane association of beta-hexosaminidase A has been directly determined by cell surface biotinylation followed by affinity chromatography purification of the biotinylated proteins, a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Mencarelli S,Cavalieri C,Magini A,Tancini B,Basso L,Lemansky P,Hasilik A,Li YT,Chigorno V,Orlacchio A,Emiliani C,Sonnino S

    更新日期:2005-10-24 00:00:00

  • Drosophila melanogaster as a model system to study long-chain fatty acid amide metabolism.

    abstract::Long-chain fatty acid amides are cell-signaling lipids identified in mammals and, recently, in invertebrates, as well. Many details regarding fatty acid amide metabolism remain unclear. Herein, we demonstrate that Drosophila melanogaster is an excellent model system for the study long-chain fatty acid amide metabolism...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Jeffries KA,Dempsey DR,Behari AL,Anderson RL,Merkler DJ

    更新日期:2014-05-02 00:00:00

  • Electrons generated by photosystem II are utilized by an oxidase in the absence of photosystem I in the cyanobacterium Synechocystis sp. PCC 6803.

    abstract::The reduction and reoxidation kinetics of the first quinone-type electron acceptor in photosystem II, QA-, were measured by fluorescence in a light-tolerant, photosystem I-less strain of the cyanobacterium Synechocystis sp. PCC 6803. In this strain, which shows excellent amplitudes of variable fluorescence, the rate o...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Vermaas WF,Shen G,Styring S

    更新日期:1994-01-03 00:00:00

  • Trichostatin A activates p18INK4c gene: differential activation and cooperation with p19INK4d gene.

    abstract::We have reported that histone deacetylase (HDAC) inhibitors activate a member of the INK4 family, the p19INK4d gene, causing G1 phase arrest. We report here that HDAC inhibitor, Trichostatin A, activates another member of the INK4 family, the p18INK4c gene, through its promoter in Jurkat cells. Interestingly, the acti...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Yokota T,Matsuzaki Y,Sakai T

    更新日期:2004-09-10 00:00:00

  • Cooperativity between platelet-activating factor and collagen in aggregation of bovine platelets III.

    abstract::In cooperative aggregation of bovine platelets induced by simultaneous addition of PAF and collagen at subthreshold concentrations the following observations were made. (i) Formation of phosphatidic acid and arachidonic acid metabolites, which characterize PAF and collagen alone-induced aggregation, respectively, was ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kojima S,Sekiya F,Inada Y,Sato F,Tsukada T,Saito Y

    更新日期:1990-07-16 00:00:00

  • Regulation of the unfolded protein response in yeast by oxidative stress.

    abstract::In the unfolded protein response (UPR), Ire1 activates Hac1 to coordinate the transcription of hundreds of genes to mitigate ER stress. Recent work in Caenorhabditis elegans suggests that oxidative stress inhibits this canonical Ire1 signalling pathway, activating instead an antioxidant stress response. We sought to d...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Guerra-Moreno A,Ang J,Welsch H,Jochem M,Hanna J

    更新日期:2019-05-01 00:00:00

  • Crystal structure of Escherichia coli SufC, an ABC-type ATPase component of the SUF iron-sulfur cluster assembly machinery.

    abstract::SufC is an ATPase component of the SUF machinery, which is involved in the biosynthesis of Fe-S clusters. To gain insight into the function of this protein, we have determined the crystal structure of Escherichia coli SufC at 2.5A resolution. Despite the similarity of the overall structure with ABC-ATPases (nucleotide...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kitaoka S,Wada K,Hasegawa Y,Minami Y,Fukuyama K,Takahashi Y

    更新日期:2006-01-09 00:00:00

  • New and unexpected routes for ultrafast electron transfer in photosynthetic reaction centers.

    abstract::In photosynthetic reaction centers, the excitation with light leads to the formation of a charge separated state across the photosynthetic membrane. For the reaction center of purple non-sulphur bacteria, it was previously generally assumed that this primary charge separation could only start with the excitation of th...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: van Brederode ME,van Grondelle R

    更新日期:1999-07-16 00:00:00