Biochemical evidence for the interaction of regulatory subunit of cAMP-dependent protein kinase with IDA (Inter-DFG-APE) region of catalytic subunit.


:To explore the structural basis required for the holoenzyme formation of cAMP-dependent protein kinase, we have prepared rabbit anti-peptide antibodies that can block the holoenzyme formation without affecting the catalytic activity of the enzyme. The antibodies were raised against a specific site in the catalytic (C)-subunit, termed IDA (Inter-DFG-APE) region, which lies between the kinase subdomains VII and VIII. Although the C-subunit immunoprecipitated with anti-IDA antibodies could not form a stable complex with regulatory (R)-subunit, it was still susceptible to inhibition by the R-subunit or by PKI, a specific inhibitor peptide containing a pseudosubstrate site. These results indicate that there exists an IDA region-mediated interaction between the R- and C-subunits, which is distinct from that mediated through the substrate site and substrate binding site. In accordance with this idea, association of synthetic IDA peptides with the R-subunit was directly demonstrated by resonance mirror analysis. The calculated association constants of IDA peptides were high enough to suggest a possible involvement of the IDA region in the initial step of holoenzyme formation.




FEBS letters


Sahara S,Sato K,Kaise H,Mori K,Sato A,Aoto M,Tokmakov AA,Fukami Y




Has Abstract


1996-04-15 00:00:00














  • Phosphatidylinositol transfer proteins: requirements in phospholipase C signaling and in regulated exocytosis.

    abstract::Phosphatidylinositol transfer proteins (PITP) are abundant cytosolic proteins originally identified because of their ability to act in vitro as specific transporters of phosphatidylinositol or phosphatidylcholine between membranes. However, the cellular function of mammalian PITP has remained enigmatic till recently. ...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Cockcroft S

    更新日期:1997-06-23 00:00:00

  • Osteopontin involvement in integrin-mediated cell signaling and regulation of expression of alkaline phosphatase during early differentiation of UMR cells.

    abstract::To clarify the function of osteopontin in osteoblast differentiation, we have examined the signal transduction pathway in an osteoblastic cell line (UMR106-6) bound to osteopontin, fibronectin, vitronectin and collagen type I surfaces. This was done by investigating the production and autophosphorylation of focal adhe...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Liu YK,Uemura T,Nemoto A,Yabe T,Fujii N,Ushida T,Tateishi T

    更新日期:1997-12-22 00:00:00

  • Deletion analysis of yeast plasma membrane H+-ATPase and identification of a regulatory domain at the carboxyl-terminus.

    abstract::The function of the amino- and carboxyl-terminal domains of the yeast plasma membrane H+-ATPase have been investigated by constructing deletions in vitro and selectively expressing the mutant enzymes in vivo. The first 27 amino acids are dispensable but deletion of a further 33 amino acids greatly decreases the appear...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Portillo F,de Larrinoa IF,Serrano R

    更新日期:1989-04-24 00:00:00

  • Screening of protein kinase inhibitors and knockdown experiments identified four kinases that affect mitochondrial ATP synthesis activity.

    abstract::Mitochondrial ATP synthase, a major ATP supplier in respiring cells, should be regulated in amount and in activity to respond to the varying demands of cells for ATP. We screened 80 protein kinase inhibitors and found that HeLa cells treated with four inhibitors exhibited reduced mitochondrial ATP synthesis activity. ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sugawara K,Fujikawa M,Yoshida M

    更新日期:2013-11-29 00:00:00

  • Chemotactic and osmotic signals share a cGMP transduction pathway in Dictyostelium discoideum.

    abstract::In the ameboid eukaryote Dictyostelium discoideum, chemotactic stimulation by cAMP induces an increase of intracellular cGMP and subsequently the phosphorylation of myosin heavy chain II. Resistance to high osmotic stress also requires transient increases of intracellular cGMP and phosphorylation of myosin heavy chain...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kuwayama H,Van Haastert PJ

    更新日期:1998-03-13 00:00:00

  • The kinetics of the reaction of superoxide radical with Fe(III) complexes of EDTA, DETAPAC and HEDTA.

    abstract::To gain an understanding of the mechanism by which the hydroxyl free radical can arise in superoxide generating systems and learn how different chelaters of iron can inhibit this reaction, a pulse radiolysis kinetic study of the reaction of O;- with Fe(III)EDTA, Fe(III)HEDTA and Fe(III)DETAPAC (or DTPA)was undertaken....

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Buettner GR,Doherty TP,Patterson LK

    更新日期:1983-07-11 00:00:00

  • alpha-Allenyl putrescine, an enzyme-activated irreversible inhibitor of bacterial and mammalian ornithine decarboxylases.

    abstract::alpha-Allenyl putrescine (5,6-heptadiene-1,4-diamine) was designed as a new potential enzyme-activated irreversible inhibitor of ornithine decarboxylase (ODC). This compound, and more specifically its (R)-enantiomer, produced time-dependent inhibitions of E. coli and rat liver ODC. The inhibitions exhibit saturation k...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Danzin C,Casara P

    更新日期:1984-09-03 00:00:00

  • Permissive role of protein kinase C alpha but not protein kinase C delta in sphingosine 1-phosphate-induced Rho A activation in C2C12 myoblasts.

    abstract::Rho GTPases participate in various important signaling pathways and have been implicated in myogenic differentiation. Here the first evidence is provided that in C2C12 myoblasts sphingosine 1-phosphate (SPP) rapidly and transiently induced membrane association of Rho A in a pertussis toxin-insensitive manner. The bioa...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Meacci E,Donati C,Cencetti F,Romiti E,Bruni P

    更新日期:2000-09-29 00:00:00

  • B cell terminal differentiation factor XBP-1 induces reactivation of Kaposi's sarcoma-associated herpesvirus.

    abstract::The herpesvirus life cycle has two distinct phases: latency and lytic replication. The viral immediate early protein replication and transcription activator (RTA) plays a central role in mediating the balance between these two phases. Here, we demonstrate that a B cell terminal differentiation factor X-box binding pro...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Yu F,Feng J,Harada JN,Chanda SK,Kenney SC,Sun R

    更新日期:2007-07-24 00:00:00

  • Regulation of oxygen consumption and microcirculation by alpha-sympathetic nerves in isolated perfused rat liver.

    abstract::In isolated rat liver perfused at constant flow with erythrocyte-free Krebs-Henseleit bicarbonate buffer containing 5 mM glucose and 2 mM lactate, perivascular stimulation of the hepatic nerves caused a rapid decrease of oxygen uptake, a decrease of the periportal and, after a transient rise, of the perivenous tissue ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ji S,Beckh K,Jungermann K

    更新日期:1984-02-13 00:00:00

  • Bicarbonate requirement for the donor side of photosystem II.

    abstract::Suppression of electron flow (and its subsequent restoration with 3-10 mM NaHCO3) on the donor side of photosystem II is shown upon either a partial depletion of pea subchloroplast membranes in bicarbonate or the addition of 5-20 microM formate. At higher concentrations (5 mM) formate induces the known 'bicarbonate ef...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Klimov VV,Allakhverdiev SI,Feyziev YaM,Baranov SV

    更新日期:1995-04-24 00:00:00

  • GDNF-Ret signaling in midbrain dopaminergic neurons and its implication for Parkinson disease.

    abstract::Glial cell line-derived neurotrophic factor (GDNF) and its canonical receptor Ret can signal together or independently to fulfill many important functions in the midbrain dopaminergic (DA) system. While Ret signaling clearly impacts on the development, maintenance and regeneration of the mesostriatal DA system, the ph...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Kramer ER,Liss B

    更新日期:2015-12-21 00:00:00

  • Activity, tissue distribution and site-directed mutagenesis of a human peptide methionine sulfoxide reductase of type B: hCBS1.

    abstract::Human CBS1 is a methionine sulfoxide reductase of type B (MSRB) as it specifically reduced Met-R-SO in peptides with dithiothreitol or the thioredoxin system as reductants. Mutation C169S in the active site completely abolished enzymatic activity, while mutation W110A only reduced activity and C105S had no effect. Lik...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Jung S,Hansel A,Kasperczyk H,Hoshi T,Heinemann SH

    更新日期:2002-09-11 00:00:00

  • The human homologue of the yeast mitochondrial AAA metalloprotease Yme1p complements a yeast yme1 disruptant.

    abstract::In yeast, three AAA superfamily metalloproteases (Yme1p, Afg3p and Rca1p) are localized to the mitochondrial inner membrane where they perform roles in the assembly and turnover of the respiratory chain complexes. We have investigated the function of the proposed human orthologue of yeast Yme1p, encoded by the YME1L g...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Shah ZH,Hakkaart GA,Arku B,de Jong L,van der Spek H,Grivell LA,Jacobs HT

    更新日期:2000-08-04 00:00:00

  • Making decisions through Myc.

    abstract::c-Myc is a transcriptional regulator involved in carcinogenesis through its role in growth control and cell cycle progression. Here we attempt to relate its role in stimulating the G1-S transition to the ability to affect functioning of key cell cycle regulators, and we focus on how its property of modulating transcri...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Nasi S,Ciarapica R,Jucker R,Rosati J,Soucek L

    更新日期:2001-02-16 00:00:00

  • Voltage sensor mutations differentially target misfolded K+ channel subunits to proteasomal and non-proteasomal disposal pathways.

    abstract::In Shaker K(+) channels, formation of an electrostatic interaction between two charged residues, D316 and K374 in transmembrane segments S3 and S4, respectively, is a key step in voltage sensor biogenesis. Mutations D316K and K374E disrupt formation of the voltage sensor and lead to endoplasmic reticulum retention. We...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Myers MP,Khanna R,Lee EJ,Papazian DM

    更新日期:2004-06-18 00:00:00

  • Form and function of the Golgi apparatus: scaffolds, cytoskeleton and signalling.

    abstract::In addition to the classical functions of the Golgi in membrane transport and glycosylation, the Golgi apparatus of mammalian cells is now recognised to contribute to the regulation of a range of cellular processes, including mitosis, DNA repair, stress responses, autophagy, apoptosis and inflammation. These processes...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Kulkarni-Gosavi P,Makhoul C,Gleeson PA

    更新日期:2019-09-01 00:00:00

  • A photo-CIDNP study of the active sites of Megasphaera elsdenii and Clostridium MP flavodoxins.

    abstract::Megasphaera elsdenii and Clostridium MP flavodoxins have been investigated by photo-CIDNP techniques. Using time-resolved spectroscopy and external dyes carrying different charges it was possible to assign unambiguously the resonance lines in the NMR-spectra to tyrosine, tryptophan and methionine residues in the two p...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Moonen CT,Hore PJ,Müller F,Kaptein R,Mayhew SG

    更新日期:1982-11-22 00:00:00

  • Targeted cleavage of HIV-1 envelope gene by a DNA enzyme and inhibition of HIV-1 envelope-CD4 mediated cell fusion.

    abstract::With the ultimate aim of developing an effective antiviral strategy against HIV-1, a mono-DNA enzyme possessing the 10-23 catalytic motif [Santoro and Joyce (1997) Proc. Natl. Acad. Sci. USA 94, 4264-4266] was synthesized against the HIV-1 envelope gene. We tested the in vitro cleavage efficiency of the 178 bp long tr...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Dash BC,Harikrishnan TA,Goila R,Shahi S,Unwalla H,Husain S,Banerjea AC

    更新日期:1998-07-24 00:00:00

  • Cell type-specific processing of human Tau proteins in Drosophila.

    abstract::Accumulation of hyperphosphorylated Tau is associated with a number of neurodegenerative diseases collectively known as tauopathies. Differences in clinical and cognitive profiles among them suggest differential sensitivity of neuronal populations to Tau levels, phosphorylation and mutations. We used tissue specific e...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Grammenoudi S,Kosmidis S,Skoulakis EM

    更新日期:2006-08-21 00:00:00

  • Tumor necrosis factor is markedly synergistic with interleukin 1 and interferon-gamma in stimulating the production of nerve growth factor in fibroblasts.

    abstract::A possible interaction between tumor necrosis factor-alpha (TNF) and other cytokines/growth factors in stimulating the production of nerve growth factor (NGF) in Swiss 3T3 cells was studied. TNF's stimulatory activity on fibroblast NGF production was synergized by interleukin-1 alpha (IL-1 alpha), IL-1 beta and interf...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hattori A,Iwasaki S,Murase K,Tsujimoto M,Sato M,Hayashi K,Kohno M

    更新日期:1994-03-07 00:00:00

  • Direction of aminoacylated transfer RNAs into antibiotic synthesis and peptidoglycan-mediated antibiotic resistance.

    abstract::Prokaryotic aminoacylated-transfer RNAs often need to be efficiently segregated between translation and other cellular biosynthetic pathways. Many clinically relevant bacteria, including Streptococcus pneumoniae, Staphylococcus aureus, Enterococcus faecalis and Pseudomonas aeruginosa direct some aminoacylated-tRNA spe...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Shepherd J,Ibba M

    更新日期:2013-09-17 00:00:00

  • Escherichia coli PII protein: purification, crystallization and oligomeric structure.

    abstract::The Escherichia coli signal transduction protein PII, product of the glnB gene, was overproduced and purified. The predicted molecular weight of the protein based on the correct nucleotide sequence is 12,427 and is very close to the value 12,435 obtained by matrix-assisted laser desorption mass spectrometry. Hexagonal...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Vasudevan SG,Gedye C,Dixon NE,Cheah E,Carr PD,Suffolk PM,Jeffrey PD,Ollis DL

    更新日期:1994-01-17 00:00:00

  • In vitro endosome-lysosome transfer of dephosphorylated EGF receptor and Shc in rat liver.

    abstract::We have studied the endosome-lysosome transfer of internalized epidermal growth factor receptor (EGFR) complexes in a cell-free system from rat liver. Analytical subfractionation of a postmitochondrial supernatant fraction showed that a pulse of internalized [(125)I]EGF was largely associated with a light endosomal fr...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Authier F,Chauvet G

    更新日期:1999-11-12 00:00:00

  • In the rat, tumor necrosis factor alpha administration results in an increase in both UCP2 and UCP3 mRNAs in skeletal muscle: a possible mechanism for cytokine-induced thermogenesis?

    abstract::Since the discovery of the new members of the UCP (uncoupling protein) family, UCP2 and UCP3, very few studies have dealt with the regulation of their expression. Bearing this in mind, administration of a single intravenous injection of TNF-alpha (100 microg/kg body weight) to rats resulted in a significant increase i...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Busquets S,Sanchís D,Alvarez B,Ricquier D,López-Soriano FJ,Argilés JM

    更新日期:1998-12-04 00:00:00

  • Investigation of H2AX methylation by the SUV39H2 protein lysine methyltransferase.

    abstract::The H3K9 protein lysine methyltransferase SUV39H2 was reported to methylate K134 of H2AX and stimulate H2AX phosphorylation during DNA damage response [Sone K et al. (2014) Nat Commun 5, 5691]. However, the sequence context of H2AX-K134 differs from the specificity of SUV39H2. We performed in vitro methylation reactio...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Schuhmacher MK,Kudithipudi S,Jeltsch A

    更新日期:2016-06-01 00:00:00

  • Tomosyn guides SNARE complex formation in coordination with Munc18 and Munc13.

    abstract::As a SNARE binding protein, tomosyn has been reported to negatively regulate synaptic exocytosis via arresting syntaxin-1 and SNAP-25 into a nonfusogenic product that precludes synaptobrevin-2 entry, raising the question how the assembly of the SNARE complex is achieved. Here, we have investigated new functions of tom...

    journal_title:FEBS letters

    pub_type: 信件


    authors: Li Y,Wang S,Li T,Zhu L,Ma C

    更新日期:2018-04-01 00:00:00

  • Improved resolution in 1H-detected 1H-15N correlation experiments.

    abstract::The determination of protein structure by NMR is restricted at molecular masses above 10 kDa by overlapping resonances. One way of overcoming this problem is to label the protein with 15N. The conventional way to record 15N spectra is to use heteronuclear multiple-quantum coherence. We present here an alternative appr...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Norwood TJ,Boyd J,Campbell ID

    更新日期:1989-09-25 00:00:00

  • Precise mapping of the tms1 binding site on p53.

    abstract::Originally identified as multicopy suppressor of a lethal growth arrest caused by expression of a tumour mutant cDNA of p53 in fission yeast the tms1 gene product was found to form stable complexes with p53 in yeast. By using purified recombinant proteins multimeric complexes of tms1 and p53 could be demonstrated and ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wagner P,Fuchs A,Prowald A,Montenarh M,Nastainczyk W

    更新日期:1995-12-18 00:00:00

  • Suppression of a phosphatidylinositol 3-kinase signal by a specific spliced variant of Drosophila PTEN.

    abstract::Drosophila PTEN (dPTEN) plays indispensable roles in the development of Drosophila melanogaster by controlling cell size and number. Although three potential spliced forms of dPTEN have been isolated, functional distinction among these forms remains elusive. In this study, we demonstrate that all spliced forms of dPTE...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Maehama T,Kosaka N,Okahara F,Takeuchi K,Umeda M,Dixon JE,Kanaho Y

    更新日期:2004-05-07 00:00:00