Abstract:
:The effects of progesterone, dehydroepiandrosterone sulfate and 17beta-estradiol on the synthesis and degradation of hyaluronate were investigated using human uterine cervix fibroblasts. The cells were incubated with [3H]glucosamine in the presence of the hormones and then [3H]hyaluronate was isolated from the medium. The changes in the radioactivity of [3H]hyaluronate showed that progesterone suppressed hyaluronate synthesis by 22% of the control levels, while dehydroepiandrosterone sulfate and 17beta-estradiol enhanced it by 22% and 12% of the control levels, respectively. Furthermore, progesterone induced degradation of high-molecular-weight [14C]hyaluronate into low-molecular-weight hyaluronate (Mr approximately 40000). These results suggest that in cultured fibroblasts from the human uterine cervix progesterone converts hyaluronate metabolism from the synthesis phase to the degradation phase.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Tanaka K,Nakamura T,Takagaki K,Funahashi M,Saito Y,Endo Mdoi
10.1016/s0014-5793(96)01538-4subject
Has Abstractpub_date
1997-02-03 00:00:00pages
223-6issue
2-3eissn
0014-5793issn
1873-3468pii
S0014-5793(96)01538-4journal_volume
402pub_type
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