Abstract:
:Fourier-transform infrared (FT-IR) spectral imaging was used for analyzing biochemical changes in tumor cells. Metabolic parameters of human lung A549/8 adenocarcinoma and U87 glioma cells were compared under stress conditions in culture along with tumor progression after cell implantation onto the chick embryo chorio-allantoic membrane. In cell culture, glucose consumption and lactic acid release were higher in U87 cells. A549/8 cells were less sensitive to oxidative stress as observed through changes in fatty acyl chains. In vivo biochemical mapping of highly (U87) vs. poorly (A549/8) angiogenic tumors provided results comparable to culture models. Therefore, FT-IR imaging allows detecting subtle chemical changes in tumors, which might be useful for diagnosis.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Petibois C,Drogat B,Bikfalvi A,Déléris G,Moenner Mdoi
10.1016/j.febslet.2007.10.052subject
Has Abstractpub_date
2007-11-27 00:00:00pages
5469-74issue
28eissn
0014-5793issn
1873-3468pii
S0014-5793(07)01120-9journal_volume
581pub_type
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