Abstract:
:alpha-Dystroglycan was quantitatively enriched from mammalian brain based on its uniform reactivity with Vicia villosa agglutinin and resolved into sub-populations possessing or lacking the sulfated glucuronic acid epitope recognized by monoclonal antibody HNK-1. We generated a new monoclonal antibody specific for a glycoepitope on brain alpha-dystroglycan but absent from alpha-dystroglycan expressed in all other tissues examined. Finally, we found that laminin-10/11 preferentially bound to brain alpha-dystroglycan compared to skeletal muscle alpha-dystroglycan. Our results suggest that tissue-specific glycosylation modifies the laminin binding specificity of alpha-dystroglycan.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
McDearmon EL,Combs AC,Sekiguchi K,Fujiwara H,Ervasti JMdoi
10.1016/j.febslet.2006.05.010subject
Has Abstractpub_date
2006-06-12 00:00:00pages
3381-5issue
14eissn
0014-5793issn
1873-3468pii
S0014-5793(06)00595-3journal_volume
580pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::One type of covalent linkages connecting lignin and hemicellulose in plant cell walls is the ester linkage between 4-O-methyl-D-glucuronic acid of glucuronoxylan and lignin alcohols. An enzyme that could hydrolyze such linkages, named glucuronoyl esterase, occurs in the cellulolytic system of the wood-rotting fungus S...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.07.041
更新日期:2007-08-21 00:00:00
abstract::Nitrate compartmentalization in intracellular organelles has been long recognized as critical for plant physiology but the molecular identity of the proteins involved remained unclear for a long time. In Arabidopsis thaliana, AtClC-a has been recently shown to be a NO(3)(-)/H(+) antiporter critical for nitrate transpo...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2009.12.042
更新日期:2010-05-17 00:00:00
abstract::Truncated cyclin A and cyclin B lacking the N-terminal domain comprising the 'destruction box' escape from proteolysis and arrest cells at metaphase. Mutation of a conserved arginine residue of the destruction domain makes cyclin B resistant to proteolysis. Here we show that mutation of the same residue also makes cyc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80844-7
更新日期:1992-07-13 00:00:00
abstract::The Arabidopsis thalina genome database was searched for homologues of the Candida cloacae fao1 gene which encodes a membrane bound, flavin-containing, hydrogen peroxide generating, long chain alcohol oxidase. This gene has not been isolated from plants or animals. Four putative candidates were found in the database b...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.07.086
更新日期:2004-09-10 00:00:00
abstract::Glutamate uptake by rat hepatocytes in primary monolayer culture was found to be mediated by a Na+-independent and by two Na+-dependent transport systems of high and low affinity. Inhibition studies with cysteate and other model amino acids rules out the participation of the neutral amino acid transport systems A, ASC...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)81024-2
更新日期:1983-09-19 00:00:00
abstract::The neopterin derivatives, neopterin and 7,8-dihydroneopterin, modulate the cellular oxidant-antioxidant balance as well as the expression of the inducible nitric oxide synthase (iNOS) gene. Since apoptosis can be induced by reactive oxygen intermediates and nitric oxide (NO) we investigated whether these neopterin de...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(96)01194-5
更新日期:1996-11-18 00:00:00
abstract::Myometrial membranes, obtained from estrogen-dominated (day 0) rat uteri, were immunoblotted with antiserum (SG1), which recognizes the alpha subunits of both Gi1 and Gi2, with antiserum (LE2) specific for Gi2 alpha, and with I3B antiserum, specific for Gi3 alpha. The data revealed the absence of detectable levels of ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80070-j
更新日期:1991-01-14 00:00:00
abstract::We studied the effect of high-fat diet on the expression and activation of the three caveolins in rat skeletal muscle and their association with the insulin signalling cascade. Initial response was characterized by increased signalling through Cav-1 and Cav-3 phosphorylation, suggesting that both participate in an ini...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.09.016
更新日期:2009-10-06 00:00:00
abstract::Endothelial monocyte-activating polypeptide II (EMAP II) is a proinflammatory cytokine and a chemoattractant for leukocytes. The mature cytokine is formed in apoptotic cells by cleavage of the precursor proEMAP II. Here we show that caspase-7 is capable of cleaving proEMAP II in vitro. A proEMAP II mutant, in which th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01777-9
更新日期:2000-01-21 00:00:00
abstract::The malaria parasite has for long been thought to escape host complement attack as a survival strategy. However, it was only recently that complement evasion mechanisms of the parasite were described. Simultaneously, the role of complement in antibody-mediated naturally acquired and vaccine-induced protection against ...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1002/1873-3468.13772
更新日期:2020-08-01 00:00:00
abstract::A method was established for measuring molecule numbers of three different myosin heavy chain (MHC) mRNA isoforms in total RNA preparations. The quantification was based on a combination of primer-directed reverse transcriptase and polymerase chain reactions with 5'-digoxigenin-labeled oligonucleotides, using external...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80523-w
更新日期:1993-03-08 00:00:00
abstract::The gene trap methodology is a powerful tool to characterize novel genes and analyze their importance in biological phenomena. It is based on the use of mouse embryonic stem cells and reporter vectors designed to randomly integrate into the genome, tagging an insertion site and generating a mutation. Theoretically, al...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/s0014-5793(00)01779-8
更新日期:2000-08-25 00:00:00
abstract::The prohormone convertase PC2 is one of the major subtilisin/kexin-like enzymes responsible for the formation of small bioactive peptides in neural and endocrine cells. This convertase is unique among the members of the subtilisin/kexin-like mammalian serine proteinase family in that it undergoes zymogen processing of...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00480-3
更新日期:1998-05-22 00:00:00
abstract::A rapid and highly efficient method of purifying the aa3-type cytochrome c oxidase from Rhodobacter sphaeroides has been developed. This method relies upon a six-histidine affinity tag fused to the C-terminus of subunit I, which confers to the oxidase a high affinity for Ni(2+)-nitrilotriacetic acid (NTA) agarose. The...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00626-k
更新日期:1995-07-10 00:00:00
abstract::In several reactions catalyzed by highly purified peptide elongation factor 1 from rabbit reticulocytes, GTP may be fully replaced by CTP but not by ATP or UTP. This holds true for the factor-dependent binding of aminoacyl-tRNA to ribosomes, GTPase activity, GTP-dependent autophosphorylation of the factor protein and ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80992-8
更新日期:1984-11-05 00:00:00
abstract::In eukaryotic cells, peroxiredoxins are both antioxidants and regulators of H(2)O(2)-mediated signaling. We previously found that peroxiredoxin-I (Prx-I) was overexpressed in non-small cell lung cancer (NSCLC) tissue. Since overexpressed protein can induce a humoral immune response, we examined whether serum from NSCL...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.04.028
更新日期:2005-05-23 00:00:00
abstract::Oxaloacetate decarboxylase of Klebsiella pneumoniae was shown to contain between 0.6 and 1.0 mol zinc per mol enzyme in different preparations. The decarboxylase activity was completely abolished after 15 min incubation with 1 mM Hg(NO3)2 in phosphate buffer, while the activity decreased only 20% if the incubation was...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80165-d
更新日期:1992-03-23 00:00:00
abstract::As a result of an experimental confluence between carcinogenesis and oxidative phosphorylation, we published in 1971 'A Unitary Hypothesis for Carcinogenesis' [(1971) J. Antibiot. Tokyo 24, 405-417]. According to this hypothesis, damaged mitochondria release mitochondrial genetic material which like that from an oncog...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81754-5
更新日期:1989-10-09 00:00:00
abstract::The TSH-induced cyclic AMP response was studied using a 3-year-old ovine thyroid cell line TSH-independent for growth: OVNIS 5H. The kinetics of cyclic AMP production was followed both in cell layers and in cell culture media, with or without phosphodiesterase inhibitor. It is noteworthy that following the first wave ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80102-8
更新日期:1986-01-06 00:00:00
abstract::The class III phosphatidylinositol 3-kinase (PI3K-III) complex and its phosphorylated lipid product phosphatidylinositol 3-phosphate (PtdIns3P) control the three topologically related membrane-involution processes autophagy, endocytosis, and cytokinesis. The activity of the catalytic unit of PI3K-III complex, the Vacu...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2012.04.046
更新日期:2012-06-04 00:00:00
abstract::Mitochondria of amoeba Acanthamoeba castellanii were used to determine the role of two energy-dissipating systems, i.e., a free fatty acid (FFA)-activated, purine nucleotide-inhibited uncoupling protein (AcUCP) and a FFA-insensitive, purine nucleotide-activated ubiquinol alternative oxidase (AcAOX), in decreasing reac...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.04.081
更新日期:2005-06-06 00:00:00
abstract::Several apolipoproteins including apoE and apoA-I are known to be associated with amyloid beta-peptide, a major component of senile plaques in Alzheimer's disease. In the present study the interaction between three human amphipathic apolipoproteins apoE3, apoA-I and apoA-II and immobilized amyloid beta-peptide (1-40) ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00206-2
更新日期:1996-03-25 00:00:00
abstract::Pleckstrin homology (PH) domains are protein modules found in proteins involved in many cellular processes. The majority of PH domain-containing proteins require membrane association for their function. It has been shown that most PH domains interact directly with the cell membrane by binding to phosphoinositides with...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/s0014-5793(01)02909-x
更新日期:2001-10-12 00:00:00
abstract::Phlorizin, a potent inhibitor of the Na+/D-glucose cotransporter, was derivatised to 3-aminophlorizin and subsequently coupled to Affi-Gel 15. Affinity chromatography of pig kidney brush border membranes solubilised in Triton X-100 allowed the purification of a 60 kDa protein on this resin. We consider this protein to...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)81315-2
更新日期:1988-07-04 00:00:00
abstract::A cDNA clone encoding cytochrome P-450(11)beta of rat adrenal has been cloned and sequenced using a bovine P-450(11)beta cDNA insert (pcP-450(11 beta)-2; (1987) J. Biochem. 102, 559-568) as a probe. The nucleotide sequence contains an open reading frame sufficient to encode the entire amino acid sequence of a P-450(11...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81053-1
更新日期:1989-09-11 00:00:00
abstract::This study was undertaken to demonstrate the unique specificity of the chemokine receptor CXCR4 antagonist AMD3100. Calcium flux assays with selected chemokine/cell combinations, affording distinct chemokine receptor specificities, revealed no interaction of AMD3100 with any of the chemokine receptors CXCR1 through CX...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03143-5
更新日期:2002-09-11 00:00:00
abstract::The promoter of the cholecystokinin (CCK) gene possesses evolutionary conserved juxtaposed E-box and cAMP/TPA responsive elements (CRE/TRE). We have examined the functional interaction of these two sites. As previously noted, c-Jun/c-Fos heterodimers greatly increase promoter activity through association with the CRE/...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00320-8
更新日期:1999-04-01 00:00:00
abstract::Deproteinized globin poly(A) + mRNAs reassociate readily in vitro with soluble RNA-binding proteins of the cytosol; reconstituted messenger ribonucleoprotein complexes are obtained which are very similar to native globin polyribosomal-mRNP as far as bouyant density in Cs2SO4 and the composition of proteins which can b...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80518-4
更新日期:1985-01-07 00:00:00
abstract::Olfactory cilia contain cyclic nucleotide-gated and Ca2+-dependent Cl- conductances that underlie excitatory chemotransduction, and a Ca2+-dependent K+ (KCa) conductance, apparently involved in inhibitory transduction. Previous single-channel patch-clamp studies on olfactory cilia revealed four different KCas, with di...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.01.079
更新日期:2005-03-14 00:00:00
abstract::HslVU is an ATP-dependent protease consisting of two multimeric components, the HslU ATPase and the HslV peptidase. To gain an insight into the role of HslVU in regulation of cell division, the reconstituted enzyme was incubated with SulA, an inhibitor of cell division in Escherichia coli, or its fusion protein with m...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00935-7
更新日期:1999-07-30 00:00:00